Araştırma Makalesi
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Tatlısu Üzerindeki Kafeslerde Üretilen Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Citrobacter freundii İzolasyonu

Yıl 2018, Cilt: 4 Sayı: 2, 85 - 89, 17.08.2018

Mustafa Türe İlyas Kutlu

https://doi.org/10.17216/limnofish.396496
Cited By: 3

Öz



Bu çalışmada, Türkiye’nin Karadeniz Bölgesi'nde kafeslerde yetiştirilen
gökkuşağı alabalıkları (Oncorhynchus
mykiss) hastalık şüphesi sonrası bakteriyolojik olarak araştırılmıştır.
Balıklardan izole edilen bakteriyel etken, hızlı teşhis kiti ile Citrobacter braakii olarak
isimlendirilmiştir (API 20E, Profil:
1704553). Bakterinin kesin tanımlanması ise 16S rRNA gen bölgesinin sekansı ile
gerçekleştirilmiştir ve bakteri Citrobacter freundii olarak
tanımlanmıştır. Ayrıca bakterinin altı farklı antimikrobiyale karşı
antibakteriyel hassasiyeti belirlenmiştir. Bakteri florfenikol, enrofloksasin,
oksitetrasiklin ve trimethoprim-sulfamethaksazol antibiyotiklerine karşı
duyarlı, eritromisin ve amoksisilin antibiyotiklerine karşı ise dirençli olarak
bulunmuştur. En etklili antibiyotik florfenikoldür. Hastalık florfenikol ile
tedavi edilmiştir.

Anahtar Kelimeler

Balık kafes citrobakteriosiz antibiyotik

Kaynakça

  • Altun S, Duman M, Büyükekiz AG, Özyiğit MO, Karataş S, Turgay E. 2013. Isolation of Citrobacter braakii from Rainbow Trout (Oncorhynchus mykiss). ISR J AQUACULT-BAMID. 1(9).
  • Austin B, Austin DA. 2007. Bacterial fish pathogens, diseases of farmed and wild fish. 4th ed. Godalming, England: Springer-Praxis 552 p. Capkin E, Terzi E, Altinok I. 2015. Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment. Dis Aquat Org. 114:127-137. doi: 10.3354/dao02852
  • CLSI 2014. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement, M100-S24.
  • Duman M, Saticioglu IB, Buyukekiz A, Altun S. 2017. Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp. isolated from diseased rainbow trout (Oncorhynchus mykiss). J GLOB ANTIMICROB RE. 10(9): 136-142. doi: 10.1016/j.jgar.2017.05.014
  • Karunasagar I, Karunasagar I, Pai R. 1992. Systemic Citrobacter freundii infection in Common carp, Cyprinus carpio L., fingerlings. J FISH DIS.15(1):95-98. doi: 10.1111/j.1365-2761.1992.tb00642.x
  • Kayis S, Capkin E, Fikri B, Altinok I. 2009. Bacteria in rainbow trout (Oncorhynchus mykiss) in the Southern Black Sea Region of Turkey - a survey. ISR J AQUACULT-BAMID. 61(4):339-344.
  • Olivier G. 2012. Disease interactions between wild and cultured fish- Perspectives from the American Northeast (Atlantic Provinces). Bull. Eur. Ass. Fish Pathol. 22(2):103-109.
  • Sanz F. 1991. Rainbow trout mortalities associated with a mixed infection with Citrobacter freundii and IPN virus. Bull. Eur. Ass. Fish Pathol. 11:222-4.
  • Sato N, Yamane N, Kawamura T. 1982. Systemic Citrobacter freundii infection among Sunfish mola mola in matsushima aquarium. Bulletin of the Japanese Society of Fisheries. 48(11):1551-1557. doi: 10.2331/suisan.48.1551
  • Tamura K, Peterson D, Peterson N, Stecher G,Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. MOL BIOL EVOL. 28(10): 2731–2739.doi: 10.1093/molbev/msr121
  • Toranzo AE, Magarinos B, Romalde JL. 2005. A review of the main bacterial fish diseases in mariculture systems. Aquaculture. 246(1-4):37-61.doi: 10.1016/j.aquaculture.2005.01.002
  • TurkStat 2016. Turkey Statistical Institute. Available from http://www.turkstat. gov.tr/Start.do, Access date: 05.02.2018.
  • Ture M, Alp H. 2016. Identification of Bacterial Pathogens and Determination of Their Antibacterial Resistance Profiles in Some Cultured Fish in Turkey. J VET RES. 60(2): 141-146. doi:10.1515/jvetres-2016-0020
  • Ture M, Altinok I, Alp H. 2018b. Effects of Cage Farming on Antimicrobial and Heavy Metal Resistance of Escherichia coli, Enterococcus faecium, and Lactococcus garvieae. Microbial Drug Resistance doi: 10.1089/mdr.2018.0040
  • Ture M, Mısır DS, Altuntaş C, Kutlu İ. 2018a. A Survey of Some Bacterial Fish Pathogens on Whiting (Merlangius merlangus euxinus) in Eastern Black Sea Coast, Turkey. Turk J Fish & Aquat Sci. 18(12):42-46. doi: 10.4194/1303-2712-v18_11_09
  • Weisburg WG, Barns SM, Pelletier DA, Lane, DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 173(2):697-703. doi:10.1128/jb.173.2.697-703.1991
  • Zhang XX, Zhang T, Fang HHP. 2009. Antibiotic resistance genes in water environment. Appl Microbiol Biotechnol. 82(3):397−414. doi: 10.1007/s00253-008-1829-z

Isolation of Citrobacter freundii from Rainbow Trout (Oncorhynchus mykiss) in Freshwater Cage

Yıl 2018, Cilt: 4 Sayı: 2, 85 - 89, 17.08.2018

Mustafa Türe İlyas Kutlu

https://doi.org/10.17216/limnofish.396496
Cited By: 3

Öz



In this study, the
bacteriological examination was done in case of disease suspect of rainbow
trout (Oncorhynchus mykiss) which kept in cages in the Black Sea Region of
Turkey. The bacterial agent was identified as Citrobacter braakii by rapid test
kit (API 20E, Profile: 1704553), further identification was performed by 16S
rRNA gene sequencing. Bacteria were identified as Citrobacter freundii by
further molecular analysis. The antibacterial susceptibility of bacteria was
also determined for 6 different antibiotics. The bacteria was sensitive to
florfenicol, enrofloxacin, oxytetracycline and trimethoprim+sulfamethoxazole
and resistant to erythromycin and amoxicillin-clavulanate. The most effective
antibiotic was florfenicol. The disease was treated with florfenicol.

Anahtar Kelimeler

Fish cage citrobacteriosis antibiotic

Kaynakça

  • Altun S, Duman M, Büyükekiz AG, Özyiğit MO, Karataş S, Turgay E. 2013. Isolation of Citrobacter braakii from Rainbow Trout (Oncorhynchus mykiss). ISR J AQUACULT-BAMID. 1(9).
  • Austin B, Austin DA. 2007. Bacterial fish pathogens, diseases of farmed and wild fish. 4th ed. Godalming, England: Springer-Praxis 552 p. Capkin E, Terzi E, Altinok I. 2015. Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment. Dis Aquat Org. 114:127-137. doi: 10.3354/dao02852
  • CLSI 2014. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement, M100-S24.
  • Duman M, Saticioglu IB, Buyukekiz A, Altun S. 2017. Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp. isolated from diseased rainbow trout (Oncorhynchus mykiss). J GLOB ANTIMICROB RE. 10(9): 136-142. doi: 10.1016/j.jgar.2017.05.014
  • Karunasagar I, Karunasagar I, Pai R. 1992. Systemic Citrobacter freundii infection in Common carp, Cyprinus carpio L., fingerlings. J FISH DIS.15(1):95-98. doi: 10.1111/j.1365-2761.1992.tb00642.x
  • Kayis S, Capkin E, Fikri B, Altinok I. 2009. Bacteria in rainbow trout (Oncorhynchus mykiss) in the Southern Black Sea Region of Turkey - a survey. ISR J AQUACULT-BAMID. 61(4):339-344.
  • Olivier G. 2012. Disease interactions between wild and cultured fish- Perspectives from the American Northeast (Atlantic Provinces). Bull. Eur. Ass. Fish Pathol. 22(2):103-109.
  • Sanz F. 1991. Rainbow trout mortalities associated with a mixed infection with Citrobacter freundii and IPN virus. Bull. Eur. Ass. Fish Pathol. 11:222-4.
  • Sato N, Yamane N, Kawamura T. 1982. Systemic Citrobacter freundii infection among Sunfish mola mola in matsushima aquarium. Bulletin of the Japanese Society of Fisheries. 48(11):1551-1557. doi: 10.2331/suisan.48.1551
  • Tamura K, Peterson D, Peterson N, Stecher G,Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. MOL BIOL EVOL. 28(10): 2731–2739.doi: 10.1093/molbev/msr121
  • Toranzo AE, Magarinos B, Romalde JL. 2005. A review of the main bacterial fish diseases in mariculture systems. Aquaculture. 246(1-4):37-61.doi: 10.1016/j.aquaculture.2005.01.002
  • TurkStat 2016. Turkey Statistical Institute. Available from http://www.turkstat. gov.tr/Start.do, Access date: 05.02.2018.
  • Ture M, Alp H. 2016. Identification of Bacterial Pathogens and Determination of Their Antibacterial Resistance Profiles in Some Cultured Fish in Turkey. J VET RES. 60(2): 141-146. doi:10.1515/jvetres-2016-0020
  • Ture M, Altinok I, Alp H. 2018b. Effects of Cage Farming on Antimicrobial and Heavy Metal Resistance of Escherichia coli, Enterococcus faecium, and Lactococcus garvieae. Microbial Drug Resistance doi: 10.1089/mdr.2018.0040
  • Ture M, Mısır DS, Altuntaş C, Kutlu İ. 2018a. A Survey of Some Bacterial Fish Pathogens on Whiting (Merlangius merlangus euxinus) in Eastern Black Sea Coast, Turkey. Turk J Fish & Aquat Sci. 18(12):42-46. doi: 10.4194/1303-2712-v18_11_09
  • Weisburg WG, Barns SM, Pelletier DA, Lane, DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 173(2):697-703. doi:10.1128/jb.173.2.697-703.1991
  • Zhang XX, Zhang T, Fang HHP. 2009. Antibiotic resistance genes in water environment. Appl Microbiol Biotechnol. 82(3):397−414. doi: 10.1007/s00253-008-1829-z
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Mustafa Türe 0000-0001-9878-826X

İlyas Kutlu 0000-0003-1744-8883

Yayımlanma Tarihi 17 Ağustos 2018
Yayımlandığı Sayı Yıl 2018Cilt: 4 Sayı: 2

Kaynak Göster

APA Türe, M., & Kutlu, İ. (2018). Isolation of Citrobacter freundii from Rainbow Trout (Oncorhynchus mykiss) in Freshwater Cage. Journal of Limnology and Freshwater Fisheries Research, 4(2), 85-89. https://doi.org/10.17216/limnofish.396496

Cited By

Identification and analysis of pathogenic bacteria causing outbreaks in Indian major carp aquaculture of Tripura
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A Novel Disease (Water Bubble Disease) of the Giant Freshwater Prawn Macrobrachium rosenbergii Caused by Citrobacter freundii: Antibiotic Treatment and Effects on the Antioxidant Enzyme Activity and Immune Responses
Antioxidants
https://doi.org/10.3390/antiox11081491
First Isolation of Acinetobacter sp. from Golden grey mullet (Liza aurata) in Turkey
Journal of Anatolian Environmental and Animal Sciences
Mustafa TÜRE
https://doi.org/10.35229/jaes.544799