Bazı Aromatik Bitki Esansiyel Yağlarının Patojenik Balık Bakterilerine Karşı Antibakteriyel Aktivitesi
Öz
24 adet bitki türünün uçucu yağları hidrodistilasyon yoluyla elde edildi
ve 7 çeşit balık patojenine (Aeromonas
hydrophila, Aeromonas salmonicida,
Vibrio anguillarum, Yersinia ruckeri, Enterococcus faecalis, Lactococcus
garvieae ve Streptococcus agalactiae)
karşı antibakteriyel etkileri araştırıldı. Uçucu yağların, disk difüzyon
yöntemiyle elde edilen antibakteriyel aktivite sonuçları, tüm patojenlere karşı
kuvvetli aktiviteleri olduğunu göstermektedir. Genel olarak, Artemisia absinthium dışında tüm
uçucu yağlar, balık patojenlerinin çoğunluğuna karşı güçlü antibakteriyel
etkiler göstermiştir. Bununla birlikte, A. absinthium uçucu yağı, sadece
A. hydrophila’ ya karşı zayıf antibakteriyel etki göstermiştir. Yirmi
dört uçucu yağ arasından çoğunlukla yedi uçucu yağ (T. spicata, T.
vulgaris, L. nobilis, C. verum, H. plicatum and A.
citriodora Paláu) tüm balık patojenlerine karşı iyi
antibakteriyel aktivite sergilemişlerdir. Test edilen antibiyotikler (furazolidon,
oksitetrasiklin, sefalotin ve trimetoprim/sulfametoksazol) ile karşılaştırıldığında, uçucu yağların
antibakteriyal etkileri çoğunlukla eşit veya güçlü olarak bulunmuştur. Uçucu
yağların antibakteriyel aktivite sonuçları göz önünde bulundurarak, su ürünleri
yetiştiriciliğinde bakteriyel balık hastalıklarına karşı antimikrobiyal
ajanların yerine alternatif olarak kullanımları uygun olabilir.
Anahtar Kelimeler
disk difüzyon Antibakteriyel aktivite balık patojenleri esansiyel yağlar
Kaynakça
- Adel M, Safari R, Ghitanchi A, Zorriehzahra M. 2016. Chemical composition and in vitro antimicrobial activity of some Iranian medical herbs against Yersinia ruckeri. Iran J Fish Sci. 15(3): 1108-1123.
- Andrews J. 2009. BSAC standardized disc susceptibility testing method (version 8). J Antimicrob Chemoth. 64(3): 454-489. doi: 10.1093/jac/dkp244
- Baydar H, Sağdiç O, Özkan G, Karadoğan T. 2004. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 15(3): 169-172. doi: 10.1016/S0956-7135(03)00028-8
- Cermelli C, Fabio A, Fabio G, Quaglio P. 2008. Effect of eucalyptus essential oil on respiratory bacteria and viruses. Curr Microbiol. 56(1): 89-92. doi: 10.1007/s00284-007-9045-0
- Conover W. 1999. Practical nonparametric statics. John Wiley & Sons, Inc., New York. 130-133.
- Görmez Ö, Diler Ö. 2017. Balık Patojenlerine Karşı Bazı Bitkisel Uçucu Yağların Antibakteriyel Aktivitesi. Yalvaç Akademi Dergisi. 2(1): 112-122.
- Gurib-Fakim A, Subratty H, Narod F, Govinden-Soulange J, Mahomoodally F. 2005. Biological activity from indigenous medicinal plants of Mauritius. Pure Appl Chem. 77(1): 41-51. doi: 10.1351/pac200577010041
- Harikrishnan R, Balasundaram C, Heo M-S. 2011. Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture. 317(1): 1-15. doi: 10.1016/j.aquaculture.2011.03.039
- Kaya I, Yigit N, Benli M. 2008. Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr J Tradit Complement Altern Med. 5(4): 363-369. doi: 10.4314/ajtcam.v5i4.31291
- Metin S, Didinen BI, Mercimek EB, Ersoy AT. 2017. Bazı Bakteriyel Balık Patojenlerine Karşı Bazı Bitkisel Uçucu Yağlarının Antibakteriyel Aktivitesi. Aquaculture Studies (Eski Yunus Araştırma Bülteni). 17(1): 59-69.
- Mousavi SM, Wilson G, Raftos D, Mirzargar SS, Omidbaigi R. 2011. Antibacterial activities of a new combination of essential oils against marine bacteria. Aquacult Int. 19(1): 205-214. doi: 10.1007/s10499-010-9354-3
- Okmen G, Ugur A, Sarac N, Arslan T. 2012. In vivo and in vitro antibacterial activities of some essential oils of Lamiaceae species on Aeromonas salmonicida isolates from cultured rainbow trout, Oncorhynchus mykiss. J Anim Feed Sci. 11(15): 2762-2768. doi: 10.3923/javaa.2012.2762.2768
- Olusola S, Emikpe B, Olaifa F. 2013. The potentials of medicinal plant extracts as bio-antimicrobials in aquaculture. Int J Med Aromat Plants. 3(3): 404-412.
- Ontas C, Esin B, Kaplaner E. 2016. Antibacterial Activity of Citrus limon Peel Essential Oil and Argania spinosa Oil Against Fish Pathogenic Bacteria. Kafkas Üniv Vet Fak. 22(5): 741-749. doi: 10.9775/kvfd.2016.15311
- Park H-M, Kim J, Chang K-S, Kim B-S, Yang Y-J, Kim G-H, Shin S-C, Park I-K. 2011. Larvicidal activity of Myrtaceae essential oils and their components against Aedes aegypti, acute toxicity on Daphnia magna, and aqueous residue. J Med Entomol. 48(2): 405-410. doi: 10.1603/ME10108
- Randrianarivelo R, Danthu P, Benoit C, Ruez P, Raherimandimby M, Sarter S. 2010. Novel alternative to antibiotics in shrimp hatchery: effects of the essential oil of Cinnamosma fragrans on survival and bacterial concentration of Penaeus monodon larvae. J Appl Microbiol. 109(2): 642-650. doi: 10.1111/j.1365-2672.2010.04694.x
- Rattanachaikunsopon P, Phumkhachorn P. 2007. Bacteriostatic effect of flavonoids isolated from leaves of Psidium guajava on fish pathogens. Fitoterapia. 78(6): 434-436. doi: 10.1016/j.fitote.2007.03.015
- Reverter M, Bontemps N, Lecchini D, Banaigs B, Sasal P. 2014. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture. 433: 50-61. doi: 10.1016/j.aquaculture.2014.05.048
- Romano L, Battaglia F, Masucci L, Sanguinetti M, Posteraro B, Plotti G, Zanetti S, Fadda G. 2005. In vitro activity of bergamot natural essence and furocoumarin-free and distilled extracts, and their associations with boric acid, against clinical yeast isolates. J Antimicrob Chemoth. 55(1): 110-114. doi: 10.1093/jac/dkh503
- Romero J, Feijoó CG, Navarrete P (2012). Antibiotics in aquaculture-use, abuse and alternatives. Health and Environment in Aquaculture. In: Carvalho ED DG, Silva RJ, editors. Rijeka, Croatia, INTECH: pp. 159-198. p.
- Roomiani L, Soltani M, Akhondzadeh BA, Mahmoodi A, Taheri MA, Yadollahi F. 2013. Evaluation of the chemical composition and in vitro antimicrobial activity of Rosmarinus officinalis, Zataria multiflora, Anethum graveolens and Eucalyptus globulus against Streptococcus iniae; the cause of zoonotic disease in farmed fish. Iran J Fish Sci. 12(3): 702-716.
- Royston P. 1995. Remark AS R94: A remark on algorithm AS 181: The W-test for normality. Journal of the Royal Statistical Society. Series C (Applied Statistics). 44(4): 547-551. doi: 10.2307/2986146
- Shapiro SS, Wilk MB. 1965. An analysis of variance test for normality (complete samples). Biometrika. 52(3/4): 591-611. doi: 10.2307/2333709
- Soltani M, Sheikhzadeh N, Ebrahimzadeh-Mousavi H, Zargar A. 2010. Effects of Zataria multiflora essential oil on innate immune responses of common carp (Cyprinus carpio). J Fish Aquat Sci. 5: 191-199. doi: 10.3923/jfas.2010.191.199
- Türker H, Yıldırım AB. 2015. Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. Biotechnol Biotec Eq. 29(2): 281-288. doi: 10.1080/13102818.2015.1006445
- Van Hai N. 2015. The use of medicinal plants as immunostimulants in aquaculture: A review. Aquaculture. 446: 88-96. doi: 10.1016/j.aquaculture.2015.03.014
- Wang JL, Meng XL, Lu RH, Wu C, Luo YT, Yan X, Li XJ, Kong XH, Nie GX. 2015. Effects of Rehmannia glutinosa on growth performance, immunological parameters and disease resistance to Aeromonas hydrophila in common carp (Cyprinus carpio L.). Aquaculture. 435: 293-300. doi: 10.1016/j.aquaculture.2014.10.004
- Zaouali Y, Bouzaine T, Boussaid M. 2010. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food Chem Toxicol. 48(11): 3144-3152. doi: 10.1016/j.fct.2010.08.010
Öz
Essential oils of twenty-four plant species were obtained by
hydrodistillation and investigated for their antibacterial effects against
seven fish pathogenic bacteria (Aeromonas hydrophila, Aeromonas
salmonicida, Vibrio anguillarum, Yersinia ruckeri, Enterococcus
faecalis, Lactococcus garvieae and Streptococcus agalactiae).
The antibacterial activity results of the essential oils obtained by disc
diffussion method showed strong activities against all pathogens. In general,
whole essential oils except Artemisia absinthium exhibited strong
antibacterial effects against the most of the fish pathogens. However, the
essential oil of A. absinthium showed weak antibacterial effect against
only A. hydrophila. Mostly seven essential oils of the plants (T.
spicata, T. vulgaris, L. nobilis, C. verum, H. plicatum
and A. citriodora Paláu) among twenty-four essential oils
exhibited good antibacterial activity against all fish pathogens. When compared
to the tested antibiotics (furazolidon, oxytetracycline, cephalothin, and
trimethoprim/sulfamethoxazole), the antibacterial effects of essential oils
were mostly obtained equivalent or stronger. Considering the antibacterial
activity results of the essential oils, their alternative use in lieu of
antimicrobial agents against bacterial fish diseases might be convenient in the
aquaculture.
Anahtar Kelimeler
disc diffusion Antibacterial activity fish pathogens essential oils
Kaynakça
- Adel M, Safari R, Ghitanchi A, Zorriehzahra M. 2016. Chemical composition and in vitro antimicrobial activity of some Iranian medical herbs against Yersinia ruckeri. Iran J Fish Sci. 15(3): 1108-1123.
- Andrews J. 2009. BSAC standardized disc susceptibility testing method (version 8). J Antimicrob Chemoth. 64(3): 454-489. doi: 10.1093/jac/dkp244
- Baydar H, Sağdiç O, Özkan G, Karadoğan T. 2004. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 15(3): 169-172. doi: 10.1016/S0956-7135(03)00028-8
- Cermelli C, Fabio A, Fabio G, Quaglio P. 2008. Effect of eucalyptus essential oil on respiratory bacteria and viruses. Curr Microbiol. 56(1): 89-92. doi: 10.1007/s00284-007-9045-0
- Conover W. 1999. Practical nonparametric statics. John Wiley & Sons, Inc., New York. 130-133.
- Görmez Ö, Diler Ö. 2017. Balık Patojenlerine Karşı Bazı Bitkisel Uçucu Yağların Antibakteriyel Aktivitesi. Yalvaç Akademi Dergisi. 2(1): 112-122.
- Gurib-Fakim A, Subratty H, Narod F, Govinden-Soulange J, Mahomoodally F. 2005. Biological activity from indigenous medicinal plants of Mauritius. Pure Appl Chem. 77(1): 41-51. doi: 10.1351/pac200577010041
- Harikrishnan R, Balasundaram C, Heo M-S. 2011. Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture. 317(1): 1-15. doi: 10.1016/j.aquaculture.2011.03.039
- Kaya I, Yigit N, Benli M. 2008. Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr J Tradit Complement Altern Med. 5(4): 363-369. doi: 10.4314/ajtcam.v5i4.31291
- Metin S, Didinen BI, Mercimek EB, Ersoy AT. 2017. Bazı Bakteriyel Balık Patojenlerine Karşı Bazı Bitkisel Uçucu Yağlarının Antibakteriyel Aktivitesi. Aquaculture Studies (Eski Yunus Araştırma Bülteni). 17(1): 59-69.
- Mousavi SM, Wilson G, Raftos D, Mirzargar SS, Omidbaigi R. 2011. Antibacterial activities of a new combination of essential oils against marine bacteria. Aquacult Int. 19(1): 205-214. doi: 10.1007/s10499-010-9354-3
- Okmen G, Ugur A, Sarac N, Arslan T. 2012. In vivo and in vitro antibacterial activities of some essential oils of Lamiaceae species on Aeromonas salmonicida isolates from cultured rainbow trout, Oncorhynchus mykiss. J Anim Feed Sci. 11(15): 2762-2768. doi: 10.3923/javaa.2012.2762.2768
- Olusola S, Emikpe B, Olaifa F. 2013. The potentials of medicinal plant extracts as bio-antimicrobials in aquaculture. Int J Med Aromat Plants. 3(3): 404-412.
- Ontas C, Esin B, Kaplaner E. 2016. Antibacterial Activity of Citrus limon Peel Essential Oil and Argania spinosa Oil Against Fish Pathogenic Bacteria. Kafkas Üniv Vet Fak. 22(5): 741-749. doi: 10.9775/kvfd.2016.15311
- Park H-M, Kim J, Chang K-S, Kim B-S, Yang Y-J, Kim G-H, Shin S-C, Park I-K. 2011. Larvicidal activity of Myrtaceae essential oils and their components against Aedes aegypti, acute toxicity on Daphnia magna, and aqueous residue. J Med Entomol. 48(2): 405-410. doi: 10.1603/ME10108
- Randrianarivelo R, Danthu P, Benoit C, Ruez P, Raherimandimby M, Sarter S. 2010. Novel alternative to antibiotics in shrimp hatchery: effects of the essential oil of Cinnamosma fragrans on survival and bacterial concentration of Penaeus monodon larvae. J Appl Microbiol. 109(2): 642-650. doi: 10.1111/j.1365-2672.2010.04694.x
- Rattanachaikunsopon P, Phumkhachorn P. 2007. Bacteriostatic effect of flavonoids isolated from leaves of Psidium guajava on fish pathogens. Fitoterapia. 78(6): 434-436. doi: 10.1016/j.fitote.2007.03.015
- Reverter M, Bontemps N, Lecchini D, Banaigs B, Sasal P. 2014. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture. 433: 50-61. doi: 10.1016/j.aquaculture.2014.05.048
- Romano L, Battaglia F, Masucci L, Sanguinetti M, Posteraro B, Plotti G, Zanetti S, Fadda G. 2005. In vitro activity of bergamot natural essence and furocoumarin-free and distilled extracts, and their associations with boric acid, against clinical yeast isolates. J Antimicrob Chemoth. 55(1): 110-114. doi: 10.1093/jac/dkh503
- Romero J, Feijoó CG, Navarrete P (2012). Antibiotics in aquaculture-use, abuse and alternatives. Health and Environment in Aquaculture. In: Carvalho ED DG, Silva RJ, editors. Rijeka, Croatia, INTECH: pp. 159-198. p.
- Roomiani L, Soltani M, Akhondzadeh BA, Mahmoodi A, Taheri MA, Yadollahi F. 2013. Evaluation of the chemical composition and in vitro antimicrobial activity of Rosmarinus officinalis, Zataria multiflora, Anethum graveolens and Eucalyptus globulus against Streptococcus iniae; the cause of zoonotic disease in farmed fish. Iran J Fish Sci. 12(3): 702-716.
- Royston P. 1995. Remark AS R94: A remark on algorithm AS 181: The W-test for normality. Journal of the Royal Statistical Society. Series C (Applied Statistics). 44(4): 547-551. doi: 10.2307/2986146
- Shapiro SS, Wilk MB. 1965. An analysis of variance test for normality (complete samples). Biometrika. 52(3/4): 591-611. doi: 10.2307/2333709
- Soltani M, Sheikhzadeh N, Ebrahimzadeh-Mousavi H, Zargar A. 2010. Effects of Zataria multiflora essential oil on innate immune responses of common carp (Cyprinus carpio). J Fish Aquat Sci. 5: 191-199. doi: 10.3923/jfas.2010.191.199
- Türker H, Yıldırım AB. 2015. Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. Biotechnol Biotec Eq. 29(2): 281-288. doi: 10.1080/13102818.2015.1006445
- Van Hai N. 2015. The use of medicinal plants as immunostimulants in aquaculture: A review. Aquaculture. 446: 88-96. doi: 10.1016/j.aquaculture.2015.03.014
- Wang JL, Meng XL, Lu RH, Wu C, Luo YT, Yan X, Li XJ, Kong XH, Nie GX. 2015. Effects of Rehmannia glutinosa on growth performance, immunological parameters and disease resistance to Aeromonas hydrophila in common carp (Cyprinus carpio L.). Aquaculture. 435: 293-300. doi: 10.1016/j.aquaculture.2014.10.004
- Zaouali Y, Bouzaine T, Boussaid M. 2010. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food Chem Toxicol. 48(11): 3144-3152. doi: 10.1016/j.fct.2010.08.010
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 17 Ağustos 2018 |
| Yayımlandığı Sayı | Yıl 2018Cilt: 4 Sayı: 2 |