Journal of Limnology and Freshwater Fisheries Research
Araştırma Makalesi
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Doğu Karadeniz Bölgesinde Amfibi Patojeni Batrachochytrium dendrobatidis’in Potansiyel Dağılımı

Yıl 2019, Cilt 5, Sayı 1, 27 - 33, 25.04.2019

Uğur Cengiz ERİŞMİŞ

https://doi.org/10.17216/limnofish.467527

Öz

Amfibi patojeni Batrachochytrium dendrobatidis Anadolu’da rapor edilmesine rağmen, hem coğrafik dağılımı hem de Türkiye amfibileri üzerindeki etkisi hala belirsizdir. Bu çalışmada, Doğu Karadeniz Bölgesindeki beş sulak alandan on farklı amfibi türlerine (Pelodytes caucasicus, Rana dalmatina, Rana macrocnemis, Bufo bufo, Bufo verrucosissimus, Bufotes variabilis, Hyla savignyi Pelophylax ridibundus, Ommatotriton ophryticus, Mertensiella caucasicus) ait 62 ergin birey toplandı. Kantitatif Real-time PCR tekniği ile tüm bireylerde B. dendrobatidis enfeksiyonlarının prevalansı ve yoğunluğu ile araştırıldı ve Doğu Karadeniz Bölgesi’ndeki 10 amfibi türünden toplanan 62 bireyin 13’ ünde B. dendrobatidis enfeksiyonu varlığı ilk kez rapor edildi. Enfeksiyon yoğunluğu 403,520-534,340 genomik eşdeğerler arasında değiştiği belirlendi. Amfibi türler arasında en yüksek genomik eşdeğer Uzungöl’ deki P. caucasicus  (534,280) ve Karagöl'de B. bufo (504,00) saptandı.

Anahtar Kelimeler

Chytridiomycosis, Batrachochytrium dendrobatidis, Anadolu, Amphibia

Kaynakça

  • Anna ES, Grismer LL, Anuar S, Onn CK, Grismer JL, Quah E, Muin MA, Ahmad N, Lenker M, Zamudio KR. 2011. First record of Batrachochytrium dendrobatidis infecting four frog families from Peninsular Malaysia. Ecohealth. 8(1):121-128. doi: 10.1007/s10393-011-0685-y
  • Ansell SW, Stenøien HK, Grundmann M, Russell SJ, Koch MA, Schneider H, Vogel JC. 2011. The importance of Anatolian mountains as the cradle of global diversity in Arabis alpina, a key arctic–alpine species. Annals of Botany. 108(2):241-252. doi: 10.1093/aob/mcr134
  • Bai C, Garner TWJ, Garner, LiY. 2010. First evidence of Batrachochytrium dendrobatidis in China: discovery of chytridiomycosis in introduced American bullfrogs and native amphibians in the Yunnan Province, China. EcoHealth. 7(1):127-134. doi: 10.1007/s10393-010-0307-0
  • Başkale E, Yildirim E,Çevik IE, Kaya U. 2013. Population size and age structure of metamorphic and pedomorphic forms of Ommatotriton ophryticus (Berthold, 1846) in the Northwestern Black Sea Region of Turkey. J Herpetol. 47(2):270-276. doi: 10.1670/11-116
  • Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD. 2004. Rapid quantitative detection of chytridiomycosis Batrachochytrium dendrobatidis in amphibian samples using real-time taqman PCR assay. Dis Aquat Organ. 60(2):141-148. doi: 10.3354/dao060141
  • Bülbül U, Matsui M, Kutrup B, Eto K. 2011. Taxonomic relationships among Turkish water frogs as revealed by phylo-genetic analyses using mtDNA gene sequences. Zool Sci. 28(12):930-936. doi: 10.2108/zsj.28.930
  • Carver S, Bell BD, Waldman B. 2010. Does chytridiomycosis disrupt amphibian skin function? Copeia. 2010(3):487-495. doi: 10.1643/CH-09-128
  • Daszak P, Berger L, Cunningham AA, Hyatt AD, Green DE, Speare R. 1999. Emerging infectious diseases and amphibian population declines. Emerg Infect Dis. 5(6):735-748. doi : 10.3201/eid0506.990601
  • Di Rosa I, Simoncelli F, Fagotti A, Pascolini R. 2007. Ecology: the proximate cause of frog declines? Nature. 447(7144):4-5. doi: 10.1038/nature05941
  • Erismis UC, Konuk M, Yoldas T, Agyar P, Yumuk D, Korcan SE. 2014. Survey of Turkey’s endemic frogs for amphibian chytrid fungus Batrachochytrium dendrobatidis. Dis Aquat Organ. 111(2):153-157. doi: 10.3354/dao02742
  • Farrer RA, Weinert LA, Bielby J, Garner TW, Balloux F, Clare F, Bosch J, Cunningham AA, Weldon C, du Preez LH, Anderson L, Pond SL, Shahar-Golan R, Henk DA, Fisher MC. 2011. Multiple emergences of genetically diverse amphibian-infecting chytrids include a globalized hypervirulent recombinant lineage. P Natl Acad Scı Usa. 108(46):18732-18736. doi: 10.1073/pnas.1111915108
  • Fisher MC, Garner TW, Walker SF. 2009. Global emergence of Batrachochytrium dendrobatidis and amphibian chytridiomycosis in space, time, and host. Annu Rev Microbiol. 63:291-310. doi: 10.1146/annurev.micro.091208.073435
  • Franzen M, Bussmann M, Kordges T, Thiesmeier B. 2008. Die Amphibien und Reptilien der Südwest-Türkei. Bielefeld: Laurenti Verlag 329 p.
  • Göçmen B, Veith M, Iğci N, Akman B, Godmann O, Wagner N. 2013. No detection of the amphibian pathogen Batrachochytrium dendrobatidis in terrestrial Turkish salamanders (Lyciasalamandra) despite its occurrence in syntopic frogs (Pelophylax bedriagae). Salamandra. 49(1):51-55.
  • Hyatt AD, Boyle DG, Olsen V, Boyle DB, Berger L, Obendorf D. 2007. Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Dis Aquat Organ. 73(3):175-192. doi: 10.3354/dao073175
  • Kriger KM, Hines HB, Hyatt AD, Boyle DG, Hero JM. 2006. Techniques for detecting chytridiomycosis in wild frogs: comparing histology with real-time taqman PCR. Dis Aquat Organ. 71(2):141-148. doi: 10.3354/dao071141
  • Kriger KM, Pereoglou F, Hero JM. 2007. Latitudinal variation in the prevalence and intensity of chytrid Batrachochytrium dendrobatidis infection in eastern Australia. Conserv Biol. 21(5):1280-1290. doi: 10.1111/j.1523-1739.2007.00777.x
  • Marantelli G, Berger L, Speare R, Keegan L 2004. Distribution of the amphibian chytrid Batrachochytrium dendrobatidis and keratin during tadpole development. Pacific Conservation Biology. 10(2):173-179. doi: 10.1071/PC040173
  • Mazzoni R, Cunningham AA, Daszak P, Apolo A, Perdomo E, Speranza G. 2003. Emerging pathogen of wild amphibians in frogs.Rana catesbeiana farmed for international trade. Emerg Infect Dis. 9(8):995-998. doi: 10.3201/eid0908.030030
  • Morehouse EA, James TY, GanleyA RD, Vilgalys R, Berger L, Murphy PJ, Longcore J E. 2003. Multilocus sequence typing suggests that the chytrid pathogen of amphibians is a recently emerged clone. Mol Ecol. 12(2):395-403. doi: 10.1046/j.1365-294X.2003.01732.x
  • Richmond JQ, Savage AE, Zamudio KR, Rosenblum EB. 2009. Toward immunogenic studies of amphibian chytridiomycosis: linking innate and acquired immunity. Bioscience 59(4):311-32. doi: 10.1525/bio.2009.59.4.9
  • Ron SR. 2005. Predicting the distribution of the amphibian pathogen Batrachochytrium dendrobatidis in the new world. Biotropica. 37(2):209-221. doi: 10.1111/j.1744-7429.2005.00028.x
  • Sensoy S, Demircan M, Ulupınar Y, Balta İ. 2008. Türkiye İklimi; [cited 18 Feb 2018]. Available from https://www.mgm.gov.tr/FILES/genel/makale/13_turkiye_iklimi.pdf
  • Şekercioğlu CH, Anderson S, Akçay E, Bilgin R, Emre Can Ö, Semiz G, Tavşanoğlu C, Baki Yokeş M, Soyumert A, İpekdal K, Sağlam İK, Yücel M, Dalfes HN. 2011. Turkey’s globally important biodiversity in crisis. Biol Conserv. 144(12):2752-2769. doi:10.1016/j.biocon.2011.06.025 Veith M, Schmidtler F, Kosuch J, Baran I, Seitz A. 2003. Paleoclimatic changes explain Anatolian mountain frog evolution: a test for alternating vicariance and dispersal event. Mol Ecol. 12(1):185-189. doi: 10.1046/j.1365-294X.2003.01714.x
  • Weldon C, du Preez LH, Hyatt AD, Muller R, Speare R. 2004. Origin of the amphibian chytrid fungus. Emerg Infect Dis. 10(12):2100-2105. doi: 10.3201/eid1012.030804
  • Yildiz MZ, Göcmen B. 2012. Population dynamics, reproduction, and life history traits of Taurus Frog, Rana holtzi Werner, 1898 (Anura: Ranidae) in Karagöl (Ulukışla, Niğde), Turkey. Herpetologica Romanica.6:1-40.

Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey

Yıl 2019, Cilt 5, Sayı 1, 27 - 33, 25.04.2019

Uğur Cengiz ERİŞMİŞ

https://doi.org/10.17216/limnofish.467527

Öz

Although Batrachochytrium dendrobatidis, pathogen for amphibians, has been reported in Anatolia, its geographical distribution, as well as its impact on the amphibians in Turkey, remained obscure. In this study, 62 adult individuals belonging to ten different species (Pelodytes caucasicus, Rana dalmatina, Rana macrocnemis, Bufo bufo, Bufo verrucosissimus, Bufotes variabilis, Hyla savignyi, Pelophylax ridibundus, Ommatotriton ophryticus, and Mertensiella caucasicus) were collected from five wetland habitats in Eastern Black Sea Region of Turkey. The prevalence and the intensity of B. dendrobatidis infections in all the individuals were investigated by using quantitative Real-time-PCR technique and the presence of B. dendrobatidis infection was reported for the first time in 13 of the 62 individuals collected from 10 amphibian species from Eastern Black Sea Region of Turkey. The intensity of B. dendrobatidis infection ranged from 403.520 to 534.280 genomic equivalents (GE) was detected. The highest GE between amphibian species were determined in P. caucasicus (534.280 GE) in Uzungöl (Çaykara-Trabzon) and B. bufo (504.00 GE) in Lake Karagöl (Şavşat-Artvin).

Anahtar Kelimeler

Chytridiomycosis, Batrachochytrium dendrobatidis, Anatolia, Amphibia

Kaynakça

  • Anna ES, Grismer LL, Anuar S, Onn CK, Grismer JL, Quah E, Muin MA, Ahmad N, Lenker M, Zamudio KR. 2011. First record of Batrachochytrium dendrobatidis infecting four frog families from Peninsular Malaysia. Ecohealth. 8(1):121-128. doi: 10.1007/s10393-011-0685-y
  • Ansell SW, Stenøien HK, Grundmann M, Russell SJ, Koch MA, Schneider H, Vogel JC. 2011. The importance of Anatolian mountains as the cradle of global diversity in Arabis alpina, a key arctic–alpine species. Annals of Botany. 108(2):241-252. doi: 10.1093/aob/mcr134
  • Bai C, Garner TWJ, Garner, LiY. 2010. First evidence of Batrachochytrium dendrobatidis in China: discovery of chytridiomycosis in introduced American bullfrogs and native amphibians in the Yunnan Province, China. EcoHealth. 7(1):127-134. doi: 10.1007/s10393-010-0307-0
  • Başkale E, Yildirim E,Çevik IE, Kaya U. 2013. Population size and age structure of metamorphic and pedomorphic forms of Ommatotriton ophryticus (Berthold, 1846) in the Northwestern Black Sea Region of Turkey. J Herpetol. 47(2):270-276. doi: 10.1670/11-116
  • Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD. 2004. Rapid quantitative detection of chytridiomycosis Batrachochytrium dendrobatidis in amphibian samples using real-time taqman PCR assay. Dis Aquat Organ. 60(2):141-148. doi: 10.3354/dao060141
  • Bülbül U, Matsui M, Kutrup B, Eto K. 2011. Taxonomic relationships among Turkish water frogs as revealed by phylo-genetic analyses using mtDNA gene sequences. Zool Sci. 28(12):930-936. doi: 10.2108/zsj.28.930
  • Carver S, Bell BD, Waldman B. 2010. Does chytridiomycosis disrupt amphibian skin function? Copeia. 2010(3):487-495. doi: 10.1643/CH-09-128
  • Daszak P, Berger L, Cunningham AA, Hyatt AD, Green DE, Speare R. 1999. Emerging infectious diseases and amphibian population declines. Emerg Infect Dis. 5(6):735-748. doi : 10.3201/eid0506.990601
  • Di Rosa I, Simoncelli F, Fagotti A, Pascolini R. 2007. Ecology: the proximate cause of frog declines? Nature. 447(7144):4-5. doi: 10.1038/nature05941
  • Erismis UC, Konuk M, Yoldas T, Agyar P, Yumuk D, Korcan SE. 2014. Survey of Turkey’s endemic frogs for amphibian chytrid fungus Batrachochytrium dendrobatidis. Dis Aquat Organ. 111(2):153-157. doi: 10.3354/dao02742
  • Farrer RA, Weinert LA, Bielby J, Garner TW, Balloux F, Clare F, Bosch J, Cunningham AA, Weldon C, du Preez LH, Anderson L, Pond SL, Shahar-Golan R, Henk DA, Fisher MC. 2011. Multiple emergences of genetically diverse amphibian-infecting chytrids include a globalized hypervirulent recombinant lineage. P Natl Acad Scı Usa. 108(46):18732-18736. doi: 10.1073/pnas.1111915108
  • Fisher MC, Garner TW, Walker SF. 2009. Global emergence of Batrachochytrium dendrobatidis and amphibian chytridiomycosis in space, time, and host. Annu Rev Microbiol. 63:291-310. doi: 10.1146/annurev.micro.091208.073435
  • Franzen M, Bussmann M, Kordges T, Thiesmeier B. 2008. Die Amphibien und Reptilien der Südwest-Türkei. Bielefeld: Laurenti Verlag 329 p.
  • Göçmen B, Veith M, Iğci N, Akman B, Godmann O, Wagner N. 2013. No detection of the amphibian pathogen Batrachochytrium dendrobatidis in terrestrial Turkish salamanders (Lyciasalamandra) despite its occurrence in syntopic frogs (Pelophylax bedriagae). Salamandra. 49(1):51-55.
  • Hyatt AD, Boyle DG, Olsen V, Boyle DB, Berger L, Obendorf D. 2007. Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Dis Aquat Organ. 73(3):175-192. doi: 10.3354/dao073175
  • Kriger KM, Hines HB, Hyatt AD, Boyle DG, Hero JM. 2006. Techniques for detecting chytridiomycosis in wild frogs: comparing histology with real-time taqman PCR. Dis Aquat Organ. 71(2):141-148. doi: 10.3354/dao071141
  • Kriger KM, Pereoglou F, Hero JM. 2007. Latitudinal variation in the prevalence and intensity of chytrid Batrachochytrium dendrobatidis infection in eastern Australia. Conserv Biol. 21(5):1280-1290. doi: 10.1111/j.1523-1739.2007.00777.x
  • Marantelli G, Berger L, Speare R, Keegan L 2004. Distribution of the amphibian chytrid Batrachochytrium dendrobatidis and keratin during tadpole development. Pacific Conservation Biology. 10(2):173-179. doi: 10.1071/PC040173
  • Mazzoni R, Cunningham AA, Daszak P, Apolo A, Perdomo E, Speranza G. 2003. Emerging pathogen of wild amphibians in frogs.Rana catesbeiana farmed for international trade. Emerg Infect Dis. 9(8):995-998. doi: 10.3201/eid0908.030030
  • Morehouse EA, James TY, GanleyA RD, Vilgalys R, Berger L, Murphy PJ, Longcore J E. 2003. Multilocus sequence typing suggests that the chytrid pathogen of amphibians is a recently emerged clone. Mol Ecol. 12(2):395-403. doi: 10.1046/j.1365-294X.2003.01732.x
  • Richmond JQ, Savage AE, Zamudio KR, Rosenblum EB. 2009. Toward immunogenic studies of amphibian chytridiomycosis: linking innate and acquired immunity. Bioscience 59(4):311-32. doi: 10.1525/bio.2009.59.4.9
  • Ron SR. 2005. Predicting the distribution of the amphibian pathogen Batrachochytrium dendrobatidis in the new world. Biotropica. 37(2):209-221. doi: 10.1111/j.1744-7429.2005.00028.x
  • Sensoy S, Demircan M, Ulupınar Y, Balta İ. 2008. Türkiye İklimi; [cited 18 Feb 2018]. Available from https://www.mgm.gov.tr/FILES/genel/makale/13_turkiye_iklimi.pdf
  • Şekercioğlu CH, Anderson S, Akçay E, Bilgin R, Emre Can Ö, Semiz G, Tavşanoğlu C, Baki Yokeş M, Soyumert A, İpekdal K, Sağlam İK, Yücel M, Dalfes HN. 2011. Turkey’s globally important biodiversity in crisis. Biol Conserv. 144(12):2752-2769. doi:10.1016/j.biocon.2011.06.025 Veith M, Schmidtler F, Kosuch J, Baran I, Seitz A. 2003. Paleoclimatic changes explain Anatolian mountain frog evolution: a test for alternating vicariance and dispersal event. Mol Ecol. 12(1):185-189. doi: 10.1046/j.1365-294X.2003.01714.x
  • Weldon C, du Preez LH, Hyatt AD, Muller R, Speare R. 2004. Origin of the amphibian chytrid fungus. Emerg Infect Dis. 10(12):2100-2105. doi: 10.3201/eid1012.030804
  • Yildiz MZ, Göcmen B. 2012. Population dynamics, reproduction, and life history traits of Taurus Frog, Rana holtzi Werner, 1898 (Anura: Ranidae) in Karagöl (Ulukışla, Niğde), Turkey. Herpetologica Romanica.6:1-40.

Ayrıntılar

Birincil Dil İngilizce
Konular Fen
Yayınlanma Tarihi April 2019
Bölüm Araştırma Makalesi
Yazarlar

Uğur Cengiz ERİŞMİŞ (Sorumlu Yazar)
Afyon Kocatepe University, Faculty of Sciences and Literatures, Moleculer Biology & Genetics Department
0000-0002-1288-3088
Türkiye

Yayımlanma Tarihi 25 Nisan 2019
Yayınlandığı Sayı Yıl 2019, Cilt 5, Sayı 1

Kaynak Göster

Bibtex @araştırma makalesi { limnofish467527, journal = {Journal of Limnology and Freshwater Fisheries Research}, eissn = {2149-4428}, address = {}, publisher = {Eğirdir Su Ürünleri Araştırma Enstitü Müdürlüğü}, year = {2019}, volume = {5}, number = {1}, pages = {27 - 33}, doi = {10.17216/limnofish.467527}, title = {Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey}, key = {cite}, author = {Erişmiş, Uğur Cengiz} }
APA Erişmiş, U. C. (2019). Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey . Journal of Limnology and Freshwater Fisheries Research , 5 (1) , 27-33 . DOI: 10.17216/limnofish.467527
MLA Erişmiş, U. C. "Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey" . Journal of Limnology and Freshwater Fisheries Research 5 (2019 ): 27-33 <http://www.limnofish.org/tr/pub/issue/44372/467527>
Chicago Erişmiş, U. C. "Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey". Journal of Limnology and Freshwater Fisheries Research 5 (2019 ): 27-33
RIS TY - JOUR T1 - Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey AU - Uğur Cengiz Erişmiş Y1 - 2019 PY - 2019 N1 - doi: 10.17216/limnofish.467527 DO - 10.17216/limnofish.467527 T2 - Journal of Limnology and Freshwater Fisheries Research JF - Journal JO - JOR SP - 27 EP - 33 VL - 5 IS - 1 SN - -2149-4428 M3 - doi: 10.17216/limnofish.467527 UR - https://doi.org/10.17216/limnofish.467527 Y2 - 2019 ER -
EndNote %0 Journal of Limnology and Freshwater Fisheries Research Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey %A Uğur Cengiz Erişmiş %T Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey %D 2019 %J Journal of Limnology and Freshwater Fisheries Research %P -2149-4428 %V 5 %N 1 %R doi: 10.17216/limnofish.467527 %U 10.17216/limnofish.467527
ISNAD Erişmiş, Uğur Cengiz . "Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey". Journal of Limnology and Freshwater Fisheries Research 5 / 1 (Nisan 2019): 27-33 . https://doi.org/10.17216/limnofish.467527
AMA Erişmiş U. C. Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey. Journal of Limnology and Freshwater Fisheries Research. 2019; 5(1): 27-33.
Vancouver Erişmiş U. C. Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey. Journal of Limnology and Freshwater Fisheries Research. 2019; 5(1): 27-33.
IEEE U. C. Erişmiş , "Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey", Journal of Limnology and Freshwater Fisheries Research, c. 5, sayı. 1, ss. 27-33, Nis. 2019, doi:10.17216/limnofish.467527