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Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Yıl 2017, Cilt: 3 Sayı: 1, 25 - 31, 24.04.2017

Yusuf Bozkurt , İlker Yavaş

https://doi.org/10.17216/limnofish.277835

Öz

Cryopreservation of sperm cells
is an essential process applied for long-term conservation of aquatic genetic
resources. The goal of this research was to determine effect different of straws volumes and thawing rates on the post-thaw
quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen
was cryopreserved according to conventional slow freezing protocol. For this
aim, the cryosolution
contained 75 mM NaCl,
70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8)
supplemented with 10% MeOH. Following equilibration at +4°C for 10 min, semen was
packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour   (for
10 min at -120ºC) and finally stored in liquid nitrogen (-196ºC) tank. Thawing of cryopreserved semen process
was performed at 30ºC for 10, 20 and 30 seconds in a water bath. Fertilization
was performed using ratio of 1x105 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved
sperm packed in 1.5 mL straws that thawed at 30ºC for 30 s. According to the
results of this research, sperm cryopreserved with ionic extender containing
10% methanol and packed in 1.5 mL straws are suitable to achieve high
fertilization of common carp eggs.

Anahtar Kelimeler

Cyprinus carpio cryopreservation spermatozoa motility straw volume thawing rate

Kaynakça

  • Akçay E, Bozkurt Y, Seçer S, Tekin N. 2004. Cryopreservation of mirror carp semen. Turk J Vet Anim Sci. 28(5):837-843.
  • Alvarez B, Fuentes R, Pimentel R, Abad Z, Cabrera E, Pimentel E, Arenal A. 2003. High fry production rates using post-thaw silver carp (Hypophthalmichtys molitrix) spermatozoa under farming conditions. Aquaculture. 220(1):195-201. doi: 10.1016/S0044-8486(02)00356-3
  • Ashwood-Smith MJ. 1980. Low-temperature preservation of cells, tissues and organs. In: Ashwood-Smith, M.J., Farrant, J. (Eds.). Low Temperature Preservation in Medicine and Biology. Pitman Medical, Turnbridge Wells, pp. 19-45.
  • Basavaraja N, Hegde SN. 2004. Cryopreservation of the endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio and storage period on post-thaw viability. Cryobiology. 49(2):149-156. doi: 10.1016/j.cryobiol.2004.05.007
  • Billard R. 1992. Reproduction in rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametes. Aquaculture. 100(1-3):263-298. doi:10.1016/0044-8486(92)90385-x
  • Bozkurt Y, Akcay E, Tekin N, Secer S. 2005. Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm. The Israeli Journal of Aquaculture-Bamidgeh. 57(2):125-130. doi: 10524/19138
  • Cabrita E, Anel L, Herráez MP. 2001. Effect of external cryoprotectants as membrane stabilizers on cryopreserved rainbow trout sperm. Theriogenology. 56(4):623–635. doi: 10.1016/S0093-691X(01)00594-5
  • Cruz-Casallas PE, Pardo-Carrasco SC, Arias-Castellanos JA, Lombo-Castellanos PE, Lombo-Rodríguez DA, Pardo-Mariño JE. 2004. Cryopreservation of Yamú Brycon siebenthalae milt. J World Aquacult Soc. 35(4):529–535. doi:10.1111/j.1749-7345.2004.tb00120.x
  • De Graaf JD, Berlinsky DL. 2004. Cryogenic and refrigerated storage of rainbow smelt (Osmerus mordax) spermatozoa. J World Aquacult Soc. 35(2):209-216. doi: 10.1111/j.1749-7345.2004.tb01076.x
  • Glogowski J, Ciereszko A, Dabrowski K. 1999. Cryopreservation of muskellunge and yellow perch semen. North Am J Aquacult. 61(3):258–262. doi: 10.1577/1548-8454(1999)0612.0.CO;2
  • Glogowski J, Kolman R, Szczeptowscki M, Horvath A, Urbanyi B, Scieczynski P, Rzemieniecki A, Domagala J, Demianowicz W, Kowalski R, Ciereszko A. 2002. Fertilizing rate of Siberian sturgeon (Acipenser baerii, Brandt) semen cryopreserved with methanol. Aquaculture.211(1):367-373. doi: 10.1016/S0044-8486(02)00003-0
  • Harvey B, Kelley RN, Ashwood-Smith MJ. 1982. Cryopreservation of zebra fish spermatozoa using methanol. Can J Zool. 60(8): 1867-1870. doi: 10.1139/z82-242
  • Horvath A, Urbanyi B. 2000. Cryopreservation of sterlet (Acipenser ruthenus) sperm. In: Norberg B, Kjesbu OS, Taranger GL, Andersson E, Stefansson SO. (Eds.), Reproductive Physiology of Fish. University of Bergen, Bergen, Norway, pp. 441 p.
  • Horvath A, Miskolczi E, Urbanyi B. 2003. Cryopreservation of common carp sperm. Aquat. Living Resour. 16(5):457-460. doi:10.1016/S0990-7440(03)00084-6
  • Lahnsteiner F, Weismann T, Patzner RA. 1996. Cryopreservation of semen of the grayling (Thymallus thymallus) and the Danube salmon (Hucho hucho). Aquaculture. 144(1-3):265–274. doi:10.1016/S0044-8486(96)01308-7
  • Lahnsteiner F, Weismann T, Patzner RA. 1997. Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of semen from salmonid fishes. Aquacult Res. 28(6):471–479. doi: 10.1046/j.1365-2109.1997.00886.x
  • Lahnsteiner F, Weismann T, Patzner RA. 1998. An efficient method for cryopreservation of testicular sperm of the Northern pike, Esox lucius Linnaeus. Aquacult Res. 29(5):341-347.
  • Lahnsteiner F, Berger B, Weismann T. 1999. Sperm metabolism of the teleost fishes Chalcalburnus chalcoides and Oncorhynchus mykiss and its relation to motility and viability. J Exp Zool. 284(4):454–465. doi: 10.1002/(SICI)1097-010X(19990901)284:4<454::AID-JEZ12>3.0.CO;2-O
  • Lahnsteiner F. 2000. Semen cryopreservation in the salmonidae and in the northern pike. Aquacult Res. 31(3):245–258. doi: 10.1046/j.1365-2109.2000.00452.x
  • Linhart O, Rodina M, Cosson J. 2000. Cryopreservation of sperm in common carp: sperm motility and hatching success of embryos. Cryobiology. 41(3):241-250. doi: 10.1006/cryo.2000.2284
  • Liu QH, Chen YK, Xiao ZZ, Li J, Xu SH, Shi XH. 2010. Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843). Aquacult Res. 41(9):89-95. doi: 10.1111/j.1365-2109.2009.02466.x
  • Muchlisin ZA, Hashim R, Chong ASC. 2004. Preliminary study on the cryopreservation of tropical bagrid catfish (Mystus nemurus) spermatozoa; the effect of extender and cryoprotectant on the motility after short-term storage. Theriogenology. 62(1-2):25-34. doi: 10.1016/j.theriogenology.2003.05.006
  • Munkittrick KR, Moccia RD. 1984. Advances in the cryopreservation of salmonid semen and suitability for a production-scale artificial fertilization program. Theriogenology. 21(4):645-659. doi:10.1016/0093-691X(84)90449-7
  • Omitogun OG, Olaniyan OF, Oyeleye OO, Ojiokpota C, Aladele SE, Odofin WT. 2010. Potentials of short term and long term cryopreserved sperm of African giant catfish (Clarias gariepinus Burchell 1822) for aquaculture. African J Biotech. 9(41): 6973-6982. doi: 10.5897/AJB09.1680
  • Rana KJ, McAndrew BJ. 1989. The viability of cryopreserved tilapia spermatozoa. Aquaculture. 76(3-4):335-345. doi: 10.1016/0044-8486(89)90085-9
  • Richardson GF, McNiven MA, Mansour N. 2011. Effect of methanol concentration and thaw rate on the viability and fertility of cryopreserved Arctic char, Salvelinus alpinus (L.), spermatozoa. Aquacult Res. 42(8):1096-1100. doi: 10.1111/j.1365-2109.2010.02695.x
  • Rodina M, Gela D, Kocour M, Hadi SMH, Hulak M, Linhart O. 2007. Cryopreservation of tench, Tinca tinca, sperm: sperm motility and hatching success of embryos. Theriogenology. 67(5):931-940. doi: 10.1016/j.theriogenology.2006.11.007
  • Steyn GJ, Van Vuren JHJ. 1987. The fertilizing capacity of cryopreserved sharptooth catfish (Clarias gariepinus) sperm. Aquaculture. 63(1-4):187-193. doi: 10.1016/0044-8486(87)90070-6
  • Stoss J, Holtz W. 1983. Cryopreservation of rainbow trout sperm: IV. The effect of DMSO concentration and equilibration time on sperm survival, sucrose and KCl as extender components and the osmolality of the thawing solution. Aquaculture. 32(3-4):321-330. doi:10.1016/0044-8486(83)90229-6
  • Suquet M, Dreanno C, Fauvel C, Cosson I, Billard R. 2000. Cryopreservation of sperm in marine fish. Aquacult Res. 31(3):231-243. doi: 10.1046/j.1365-2109.2000.00445.x
  • Tekin N, Seçer S, Akçay E, Bozkurt Y, Kayam S. 2007. Effects of glycerol additions on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectant. J Appl Ichthyol. 23(1):60-63. doi:10.1111/j.1439-0426.2006.00792.x
  • Tiersch, T. R., C. A. Goudie, and G. J. Carmichael. 1994. Cryopreservation of channel catfish sperm: storage in cryoprotectants, fertilization trials, and growth of channel catfish produced with cryopreserved sperm. T Am Fish Soc. 123(4):580-586. doi:10.1577/1548-8659(1994)123<0580:COCCSS>2.3.CO;2
  • Velasco-Santamaria YM, Medina-Robles VM, Cruz-Casallas PE. 2006. Cryopreservation of yamu (Brycon amazonicus) sperm for large scale fertilization. Aquaculture. 256(1-4): 264-271. doi: 10.1016/j.aquaculture.2006.02.039
  • Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K. 2009. A sperm cryopreservation protocol for the loach (Misgurnus anguillicaudatus) and its applicability for other related species. Theriogenology. 116:3-4. doi: 10.1016/j.anireprosci.2009.02.021
  • Yavaş İ, Bozkurt Y. 2011. Effect of different thawing rates on motility and fertilizing capacity of cryopreserved grass carp (Ctenopharyngodon idella) sperm. Biotechnol Biotec Eq. 25(1): 2254-2257. doi:10.5504/BBEQ.2011.0018

Yıl 2017, Cilt: 3 Sayı: 1, 25 - 31, 24.04.2017

Yusuf Bozkurt , İlker Yavaş

https://doi.org/10.17216/limnofish.277835

Öz

Kaynakça

  • Akçay E, Bozkurt Y, Seçer S, Tekin N. 2004. Cryopreservation of mirror carp semen. Turk J Vet Anim Sci. 28(5):837-843.
  • Alvarez B, Fuentes R, Pimentel R, Abad Z, Cabrera E, Pimentel E, Arenal A. 2003. High fry production rates using post-thaw silver carp (Hypophthalmichtys molitrix) spermatozoa under farming conditions. Aquaculture. 220(1):195-201. doi: 10.1016/S0044-8486(02)00356-3
  • Ashwood-Smith MJ. 1980. Low-temperature preservation of cells, tissues and organs. In: Ashwood-Smith, M.J., Farrant, J. (Eds.). Low Temperature Preservation in Medicine and Biology. Pitman Medical, Turnbridge Wells, pp. 19-45.
  • Basavaraja N, Hegde SN. 2004. Cryopreservation of the endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio and storage period on post-thaw viability. Cryobiology. 49(2):149-156. doi: 10.1016/j.cryobiol.2004.05.007
  • Billard R. 1992. Reproduction in rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametes. Aquaculture. 100(1-3):263-298. doi:10.1016/0044-8486(92)90385-x
  • Bozkurt Y, Akcay E, Tekin N, Secer S. 2005. Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm. The Israeli Journal of Aquaculture-Bamidgeh. 57(2):125-130. doi: 10524/19138
  • Cabrita E, Anel L, Herráez MP. 2001. Effect of external cryoprotectants as membrane stabilizers on cryopreserved rainbow trout sperm. Theriogenology. 56(4):623–635. doi: 10.1016/S0093-691X(01)00594-5
  • Cruz-Casallas PE, Pardo-Carrasco SC, Arias-Castellanos JA, Lombo-Castellanos PE, Lombo-Rodríguez DA, Pardo-Mariño JE. 2004. Cryopreservation of Yamú Brycon siebenthalae milt. J World Aquacult Soc. 35(4):529–535. doi:10.1111/j.1749-7345.2004.tb00120.x
  • De Graaf JD, Berlinsky DL. 2004. Cryogenic and refrigerated storage of rainbow smelt (Osmerus mordax) spermatozoa. J World Aquacult Soc. 35(2):209-216. doi: 10.1111/j.1749-7345.2004.tb01076.x
  • Glogowski J, Ciereszko A, Dabrowski K. 1999. Cryopreservation of muskellunge and yellow perch semen. North Am J Aquacult. 61(3):258–262. doi: 10.1577/1548-8454(1999)0612.0.CO;2
  • Glogowski J, Kolman R, Szczeptowscki M, Horvath A, Urbanyi B, Scieczynski P, Rzemieniecki A, Domagala J, Demianowicz W, Kowalski R, Ciereszko A. 2002. Fertilizing rate of Siberian sturgeon (Acipenser baerii, Brandt) semen cryopreserved with methanol. Aquaculture.211(1):367-373. doi: 10.1016/S0044-8486(02)00003-0
  • Harvey B, Kelley RN, Ashwood-Smith MJ. 1982. Cryopreservation of zebra fish spermatozoa using methanol. Can J Zool. 60(8): 1867-1870. doi: 10.1139/z82-242
  • Horvath A, Urbanyi B. 2000. Cryopreservation of sterlet (Acipenser ruthenus) sperm. In: Norberg B, Kjesbu OS, Taranger GL, Andersson E, Stefansson SO. (Eds.), Reproductive Physiology of Fish. University of Bergen, Bergen, Norway, pp. 441 p.
  • Horvath A, Miskolczi E, Urbanyi B. 2003. Cryopreservation of common carp sperm. Aquat. Living Resour. 16(5):457-460. doi:10.1016/S0990-7440(03)00084-6
  • Lahnsteiner F, Weismann T, Patzner RA. 1996. Cryopreservation of semen of the grayling (Thymallus thymallus) and the Danube salmon (Hucho hucho). Aquaculture. 144(1-3):265–274. doi:10.1016/S0044-8486(96)01308-7
  • Lahnsteiner F, Weismann T, Patzner RA. 1997. Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of semen from salmonid fishes. Aquacult Res. 28(6):471–479. doi: 10.1046/j.1365-2109.1997.00886.x
  • Lahnsteiner F, Weismann T, Patzner RA. 1998. An efficient method for cryopreservation of testicular sperm of the Northern pike, Esox lucius Linnaeus. Aquacult Res. 29(5):341-347.
  • Lahnsteiner F, Berger B, Weismann T. 1999. Sperm metabolism of the teleost fishes Chalcalburnus chalcoides and Oncorhynchus mykiss and its relation to motility and viability. J Exp Zool. 284(4):454–465. doi: 10.1002/(SICI)1097-010X(19990901)284:4<454::AID-JEZ12>3.0.CO;2-O
  • Lahnsteiner F. 2000. Semen cryopreservation in the salmonidae and in the northern pike. Aquacult Res. 31(3):245–258. doi: 10.1046/j.1365-2109.2000.00452.x
  • Linhart O, Rodina M, Cosson J. 2000. Cryopreservation of sperm in common carp: sperm motility and hatching success of embryos. Cryobiology. 41(3):241-250. doi: 10.1006/cryo.2000.2284
  • Liu QH, Chen YK, Xiao ZZ, Li J, Xu SH, Shi XH. 2010. Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843). Aquacult Res. 41(9):89-95. doi: 10.1111/j.1365-2109.2009.02466.x
  • Muchlisin ZA, Hashim R, Chong ASC. 2004. Preliminary study on the cryopreservation of tropical bagrid catfish (Mystus nemurus) spermatozoa; the effect of extender and cryoprotectant on the motility after short-term storage. Theriogenology. 62(1-2):25-34. doi: 10.1016/j.theriogenology.2003.05.006
  • Munkittrick KR, Moccia RD. 1984. Advances in the cryopreservation of salmonid semen and suitability for a production-scale artificial fertilization program. Theriogenology. 21(4):645-659. doi:10.1016/0093-691X(84)90449-7
  • Omitogun OG, Olaniyan OF, Oyeleye OO, Ojiokpota C, Aladele SE, Odofin WT. 2010. Potentials of short term and long term cryopreserved sperm of African giant catfish (Clarias gariepinus Burchell 1822) for aquaculture. African J Biotech. 9(41): 6973-6982. doi: 10.5897/AJB09.1680
  • Rana KJ, McAndrew BJ. 1989. The viability of cryopreserved tilapia spermatozoa. Aquaculture. 76(3-4):335-345. doi: 10.1016/0044-8486(89)90085-9
  • Richardson GF, McNiven MA, Mansour N. 2011. Effect of methanol concentration and thaw rate on the viability and fertility of cryopreserved Arctic char, Salvelinus alpinus (L.), spermatozoa. Aquacult Res. 42(8):1096-1100. doi: 10.1111/j.1365-2109.2010.02695.x
  • Rodina M, Gela D, Kocour M, Hadi SMH, Hulak M, Linhart O. 2007. Cryopreservation of tench, Tinca tinca, sperm: sperm motility and hatching success of embryos. Theriogenology. 67(5):931-940. doi: 10.1016/j.theriogenology.2006.11.007
  • Steyn GJ, Van Vuren JHJ. 1987. The fertilizing capacity of cryopreserved sharptooth catfish (Clarias gariepinus) sperm. Aquaculture. 63(1-4):187-193. doi: 10.1016/0044-8486(87)90070-6
  • Stoss J, Holtz W. 1983. Cryopreservation of rainbow trout sperm: IV. The effect of DMSO concentration and equilibration time on sperm survival, sucrose and KCl as extender components and the osmolality of the thawing solution. Aquaculture. 32(3-4):321-330. doi:10.1016/0044-8486(83)90229-6
  • Suquet M, Dreanno C, Fauvel C, Cosson I, Billard R. 2000. Cryopreservation of sperm in marine fish. Aquacult Res. 31(3):231-243. doi: 10.1046/j.1365-2109.2000.00445.x
  • Tekin N, Seçer S, Akçay E, Bozkurt Y, Kayam S. 2007. Effects of glycerol additions on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectant. J Appl Ichthyol. 23(1):60-63. doi:10.1111/j.1439-0426.2006.00792.x
  • Tiersch, T. R., C. A. Goudie, and G. J. Carmichael. 1994. Cryopreservation of channel catfish sperm: storage in cryoprotectants, fertilization trials, and growth of channel catfish produced with cryopreserved sperm. T Am Fish Soc. 123(4):580-586. doi:10.1577/1548-8659(1994)123<0580:COCCSS>2.3.CO;2
  • Velasco-Santamaria YM, Medina-Robles VM, Cruz-Casallas PE. 2006. Cryopreservation of yamu (Brycon amazonicus) sperm for large scale fertilization. Aquaculture. 256(1-4): 264-271. doi: 10.1016/j.aquaculture.2006.02.039
  • Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K. 2009. A sperm cryopreservation protocol for the loach (Misgurnus anguillicaudatus) and its applicability for other related species. Theriogenology. 116:3-4. doi: 10.1016/j.anireprosci.2009.02.021
  • Yavaş İ, Bozkurt Y. 2011. Effect of different thawing rates on motility and fertilizing capacity of cryopreserved grass carp (Ctenopharyngodon idella) sperm. Biotechnol Biotec Eq. 25(1): 2254-2257. doi:10.5504/BBEQ.2011.0018
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Bölüm Araştırma Makalesi
Yazarlar

Yusuf Bozkurt

İlker Yavaş

Yayımlanma Tarihi 24 Nisan 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 3 Sayı: 1

Kaynak Göster

APA Bozkurt, Y., & Yavaş, İ. (2017). Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm. Journal of Limnology and Freshwater Fisheries Research, 3(1), 25-31. https://doi.org/10.17216/limnofish.277835