Journal of Limnology and Freshwater Fisheries Research
Research Article
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Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey

Year 2021, Volume 7, Issue 2, 101 - 107, 26.08.2021

Şevki KAYIŞ Gökhan SOYKÖSE Zeynep Zehra İPEK Akif ER

https://doi.org/10.17216/limnofish.827718
Cited By: 1

Abstract

This study was carried out to reveal bacterial contamination and antibiotic resistance profiles of isolated bacteria in the hatchery systems of trout farms located in the Eastern Black Sea region of Turkey. Inlet water, egg, alevin and fry samples of 11 different trout farms were examined in terms of bacterial contamination in four different basins. After sampling, isolated bacteria were identified by making phenotypic and genotypic analyses. The majority of a total of 43 strains belongs to the genera Aeromonas and Pseudomonas, but also Lelliottia sp., Bacillus sp. and Lactococcus lactis were isolated from hatchery systems. Considering all basins, except for the 2nd basin, the highest antibiotic resistance of bacteria was against Ampicillin. The lowest antibiotic resistance percentages were determined against gentamicin and enrofloxacin. As a result of the research, the detection of different bacteria in the samples taken from the inlet water of fish farms showed presence of different bacteria contaminating the hatchery water. It thereby stressed the need for improved hygiene measures in these farms.

Keywords

antibiotic, bacteria, trout, hatchery

References

  • Altınok İ, Kayış Ş, Çapkın E. 2006. Pseudomonas putida infection in rainbow trout. Aquaculture. 261(3):850-855. doi: 10.1016/j.aquaculture.2006.09.009
  • Altınok İ, Balta F, Çapkın E, Kayış Ş. 2007. Disease of rainbow trout caused by Pseudomonas luteola. Aquaculture. 273(4):393-397. doi: 10.1016/j.aquaculture.2007.10.025
  • Austin B, Austin DA. 2007 Bacterial fish pathogens: diseases of farmed and wild fish, 4. Edition Springer Publishing, New York, 545p.
  • Balta F, Kayış Ş, Altınok İ. 2008. External protozoan parasites in three trout species in the Eastern Black Sea region of the Turkey: intensity, seasonality, and their treatments. B Eur Assoc Fish Pat. 28(4):157-162.
  • Barrie D, Hoffman PN, Wilson JA, Kramer JM. 1994. Contamination of hospital linen by Bacillus cereus. Epidemiol Infect. 113(2):297-306. doi: 10.1017/s0950268800051724
  • Brown LL, Cox WT, Levine RP. 1997. Evidence that the causal agent of bacterial cold-water disease Flavobacterium psychrophilum is transmitted within salmonid eggs. Dis Aquat Organ. 29:213-218. doi: 10.3354/dao029213
  • Cappuccino JG, Sherman N. 1992. Microbiology, A Laboratory Manual, 3rd ed. New York, the USA: Rockland Community College, Suffern 76 p.
  • CLSI 2018. Clinical and Laboratory Standart Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. Wayne, the USA: Clinical and Laboratory Standart Institute 112 p.
  • Evelyn TPT, Prosperi-Porta L, Ketcheson JE. 1986. Experimental intra-ovum infection of salmonid eggs with Renibacterium salmoninarum and vertical transmission of the pathogen with such eggs despite their treatment with erythromycin. Dis Aquat Organ. 1(3):197-202. doi: 10.3354/DAO001197
  • Fiorentini C, Barbieri E, Falzano L, Matarrese P, Baffone W, Pianetti A, Katouli M, Kühn I, Möllby R, Bruscolini F, Casiere A, Doneli G. 1998. Occurrence, diversity and pathogenicity of mesophilic Aeromonas in estuarine waters of the Italian coast of the Adriatic Sea. J Appl Microbiol. 85(3):501-511. doi: 10.1046/j.1365-2672.1998.853517.x
  • Gavriel AA, Landre JP, Lamb AJ. 1998. Incidence of mesophilic Aeromonas within a public drinking water supply in North-East Scotland. J Appl Microbiol. 84(3):383-392. doi: 10.1046/j.1365-2672.1998.00354.x
  • Gedik K, Verep B, Terzi E, Fevzioğlu S. 2010. Fırtına Deresi (Rize)'nin Fiziko-Kimyasal Açıdan Su Kalitesinin Belirlenmesi. Ekoloji 76:25-35. doi: 10.5053/ekoloji.2010.764
  • Gün A, Kızak V. 2019. Dünyada ve Türkiye’de Su Ürünleri Üretiminde İstatistiki Durum. Menba Journal of Fisheries Faculty. 5(2):25-36.
  • Gür D. 1996. Aminoglikozit antibiyotiklere direnç mekanizmaları ve Türkiye’deki durum. Mikrobiyol Bült. 30:197-204.
  • Hanninen ML, Siitonen A. 1995. Distribution of Aeromonas phenospecies and genospecies among strains isolated from water, foods or from human clinical samples. Epidemiol Infect. 115(1):39-50. doi: 10.1017/S0950268800058106
  • Işıdan H. 2006. Önemli Bazı Viral Balık Hastalıkları-II. SÜMAE Yunus Araştırma Bülteni. 6(2):10-13.
  • Kayış S. 2019. Analysis of Fish Health Status in Terms of Sustainability of Aquaculture in Turkey-A SWOT Analysis. Aquaculture Studies. 19(1):69-76. doi: 10.4194/2618-6381-v19_1_07
  • Kayış Ş, Mihai PS, Kurtoğlu İ. 2014. Experimental infections of Pseudomonas putida and Pseudomonas fluorescens in rainbow trout (Oncorhynchus mykiss) alevins. El-Cezerî Journal of Science and Engineering. 1(1):7-11.
  • Kayış Ş, Er A, Yılmaz C, Düzgün A, Köse Ö, Kurtoğlu IZ. 2015. Aeromonas hydrophila as a causative agent of blue sac fry syndrome in different trout species. J Fish Dis. 38(12):1069-1071. doi: 10.1111/jfd.12326
  • Kayış Ş, Er A, Kangel P, Kurtoğlu IZ. 2017. Bacterial pathogens and health problems of Acipenser gueldenstaedtii and Acipenser baerii sturgeons reared in the eastern Black Sea region of Turkey. Iran J Vet Res. 18(1):18-24.
  • Lasee BA. 1995. Introduction to fish health management, 2nd ed. Wisconsin: U.S Fish and Wildlife Service 139 p.
  • Mena KD, Gerba CP. 2009. Risk assessment of Pseudomonas aeruginosa in water. Rev Environ Contam T. 201:71-115. doi: 10.1007/978-1-4419-0032-6_3
  • Onuk EE, Tanrıverdi Çaycı Y, Çoban AY, Çiftçi A, Balta F, Didinen BI, Altun S. 2017. Balık ve yetiştirme suyu kökenli Aeromonas izolatlarının antimikrobiyal duyarlılıklarının saptanması. Ankara Üniv Vet Fak Der. 64:69-73. doi: 10.1501/Vetfak_0000002777
  • Ural MŞ, Çalta M, Celayir Y, Aydın R. 2011. Gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum 1972) yumurtalarının dezenfeksiyonunda kullanılan bazı kimyasal maddelerin kuluçka parametrelerine etkisi. Biyoloji Bilimleri Araştırma Dergisi. 4(1):37-41.
  • Yılmaz E, Yılmaz A, Bilgin B. 2011. Alabalık Kuluçkahanelerinde Görülen Önemli Hastalıklar ve Tedavi Yöntemleri. Türk Bilimsel Derlemeler Dergisi. 4(2):37-39.

Türkiye'nin Doğu Karadeniz Bölgesinde Bulunan Bazı Alabalık Çiftliklerinin Kuluçkahanelerinde Bakteri Kontaminasyonu ve Bakterilerin Antibiyotik Direncinin Belirlenmesi

Year 2021, Volume 7, Issue 2, 101 - 107, 26.08.2021

Şevki KAYIŞ Gökhan SOYKÖSE Zeynep Zehra İPEK Akif ER

https://doi.org/10.17216/limnofish.827718
Cited By: 1

Abstract

Bu çalışma, Türkiye'nin Doğu Karadeniz bölgesinde bulunan alabalık çiftliklerinin kuluçkahane sistemlerinden izole edilen bakterilerin kontaminasyonu ve antibiyotik direnç profillerini ortaya çıkarmak için yapılmıştır. 11 farklı alabalık çiftliğinin giriş suyu, yumurta, alevin ve yavru örnekleri, dört farklı havzada bakteriyel kontaminasyon açısından incelenmiştir. Örneklemeden sonra izole edilen bakteriler fenotipik ve genotipik analizler yapılarak teşhis edilmiştir. Toplam 43 suşun çoğunluğu Aeromonas ve Pseudomonas cinsinlerine aittir, fakat aynı zamanda Lelliottia sp., Bacillus sp. ve Lactococcus lactis, kuluçka sistemlerinden izole edilmiştir. 2. havza hariç tüm havzalara bakıldığında bakterilerin en yüksek antibiyotik direnci Ampisiline karşı olmuştur. En düşük antibiyotik direnç yüzdeleri gentamisin ve enrofloksasine karşı belirlenmiştir. Araştırma sonucunda, balık çiftliklerinin giriş sularından alınan örneklerde farklı bakterilerin tespiti, kuluçkahane suyunu farklı bakterilerin kontamine ettiğini göstermiştir. Böylelikle bu çiftliklerde hijyen önlemlerinin iyileştirilmesi ihtiyacı belirlenmiştir.

Keywords

Antibiyotik, bakteri, alabalık, kuluçkahane

References

  • Altınok İ, Kayış Ş, Çapkın E. 2006. Pseudomonas putida infection in rainbow trout. Aquaculture. 261(3):850-855. doi: 10.1016/j.aquaculture.2006.09.009
  • Altınok İ, Balta F, Çapkın E, Kayış Ş. 2007. Disease of rainbow trout caused by Pseudomonas luteola. Aquaculture. 273(4):393-397. doi: 10.1016/j.aquaculture.2007.10.025
  • Austin B, Austin DA. 2007 Bacterial fish pathogens: diseases of farmed and wild fish, 4. Edition Springer Publishing, New York, 545p.
  • Balta F, Kayış Ş, Altınok İ. 2008. External protozoan parasites in three trout species in the Eastern Black Sea region of the Turkey: intensity, seasonality, and their treatments. B Eur Assoc Fish Pat. 28(4):157-162.
  • Barrie D, Hoffman PN, Wilson JA, Kramer JM. 1994. Contamination of hospital linen by Bacillus cereus. Epidemiol Infect. 113(2):297-306. doi: 10.1017/s0950268800051724
  • Brown LL, Cox WT, Levine RP. 1997. Evidence that the causal agent of bacterial cold-water disease Flavobacterium psychrophilum is transmitted within salmonid eggs. Dis Aquat Organ. 29:213-218. doi: 10.3354/dao029213
  • Cappuccino JG, Sherman N. 1992. Microbiology, A Laboratory Manual, 3rd ed. New York, the USA: Rockland Community College, Suffern 76 p.
  • CLSI 2018. Clinical and Laboratory Standart Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. Wayne, the USA: Clinical and Laboratory Standart Institute 112 p.
  • Evelyn TPT, Prosperi-Porta L, Ketcheson JE. 1986. Experimental intra-ovum infection of salmonid eggs with Renibacterium salmoninarum and vertical transmission of the pathogen with such eggs despite their treatment with erythromycin. Dis Aquat Organ. 1(3):197-202. doi: 10.3354/DAO001197
  • Fiorentini C, Barbieri E, Falzano L, Matarrese P, Baffone W, Pianetti A, Katouli M, Kühn I, Möllby R, Bruscolini F, Casiere A, Doneli G. 1998. Occurrence, diversity and pathogenicity of mesophilic Aeromonas in estuarine waters of the Italian coast of the Adriatic Sea. J Appl Microbiol. 85(3):501-511. doi: 10.1046/j.1365-2672.1998.853517.x
  • Gavriel AA, Landre JP, Lamb AJ. 1998. Incidence of mesophilic Aeromonas within a public drinking water supply in North-East Scotland. J Appl Microbiol. 84(3):383-392. doi: 10.1046/j.1365-2672.1998.00354.x
  • Gedik K, Verep B, Terzi E, Fevzioğlu S. 2010. Fırtına Deresi (Rize)'nin Fiziko-Kimyasal Açıdan Su Kalitesinin Belirlenmesi. Ekoloji 76:25-35. doi: 10.5053/ekoloji.2010.764
  • Gün A, Kızak V. 2019. Dünyada ve Türkiye’de Su Ürünleri Üretiminde İstatistiki Durum. Menba Journal of Fisheries Faculty. 5(2):25-36.
  • Gür D. 1996. Aminoglikozit antibiyotiklere direnç mekanizmaları ve Türkiye’deki durum. Mikrobiyol Bült. 30:197-204.
  • Hanninen ML, Siitonen A. 1995. Distribution of Aeromonas phenospecies and genospecies among strains isolated from water, foods or from human clinical samples. Epidemiol Infect. 115(1):39-50. doi: 10.1017/S0950268800058106
  • Işıdan H. 2006. Önemli Bazı Viral Balık Hastalıkları-II. SÜMAE Yunus Araştırma Bülteni. 6(2):10-13.
  • Kayış S. 2019. Analysis of Fish Health Status in Terms of Sustainability of Aquaculture in Turkey-A SWOT Analysis. Aquaculture Studies. 19(1):69-76. doi: 10.4194/2618-6381-v19_1_07
  • Kayış Ş, Mihai PS, Kurtoğlu İ. 2014. Experimental infections of Pseudomonas putida and Pseudomonas fluorescens in rainbow trout (Oncorhynchus mykiss) alevins. El-Cezerî Journal of Science and Engineering. 1(1):7-11.
  • Kayış Ş, Er A, Yılmaz C, Düzgün A, Köse Ö, Kurtoğlu IZ. 2015. Aeromonas hydrophila as a causative agent of blue sac fry syndrome in different trout species. J Fish Dis. 38(12):1069-1071. doi: 10.1111/jfd.12326
  • Kayış Ş, Er A, Kangel P, Kurtoğlu IZ. 2017. Bacterial pathogens and health problems of Acipenser gueldenstaedtii and Acipenser baerii sturgeons reared in the eastern Black Sea region of Turkey. Iran J Vet Res. 18(1):18-24.
  • Lasee BA. 1995. Introduction to fish health management, 2nd ed. Wisconsin: U.S Fish and Wildlife Service 139 p.
  • Mena KD, Gerba CP. 2009. Risk assessment of Pseudomonas aeruginosa in water. Rev Environ Contam T. 201:71-115. doi: 10.1007/978-1-4419-0032-6_3
  • Onuk EE, Tanrıverdi Çaycı Y, Çoban AY, Çiftçi A, Balta F, Didinen BI, Altun S. 2017. Balık ve yetiştirme suyu kökenli Aeromonas izolatlarının antimikrobiyal duyarlılıklarının saptanması. Ankara Üniv Vet Fak Der. 64:69-73. doi: 10.1501/Vetfak_0000002777
  • Ural MŞ, Çalta M, Celayir Y, Aydın R. 2011. Gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum 1972) yumurtalarının dezenfeksiyonunda kullanılan bazı kimyasal maddelerin kuluçka parametrelerine etkisi. Biyoloji Bilimleri Araştırma Dergisi. 4(1):37-41.
  • Yılmaz E, Yılmaz A, Bilgin B. 2011. Alabalık Kuluçkahanelerinde Görülen Önemli Hastalıklar ve Tedavi Yöntemleri. Türk Bilimsel Derlemeler Dergisi. 4(2):37-39.

Details

Primary Language English
Subjects Fisheries
Published Date August 2021
Journal Section Research Article
Authors

Şevki KAYIŞ (Primary Author)
Recep Tayyip Erdoğan Üniversitesi Su ürünleri Fakültesi
0000-0002-9391-7613
Türkiye

Gökhan SOYKÖSE This is me
RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ
0000-0003-4249-4304
Türkiye

Zeynep Zehra İPEK
RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ
0000-0002-5916-1891
Türkiye

Akif ER
RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ
0000-0002-0052-5590
Türkiye

Publication Date August 26, 2021
Published in Issue Year 2021, Volume 7, Issue 2

Cite

Bibtex @research article { limnofish827718, journal = {Journal of Limnology and Freshwater Fisheries Research}, issn = {}, eissn = {2149-4428}, address = {}, publisher = {Egirdir Fisheries Research Institute}, year = {2021}, volume = {7}, pages = {101 - 107}, doi = {10.17216/limnofish.827718}, title = {Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey}, key = {cite}, author = {Kayış, Şevki and Soyköse, Gökhan and İpek, Zeynep Zehra and Er, Akif} }
APA Kayış, Ş. , Soyköse, G. , İpek, Z. Z. & Er, A. (2021). Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey . Journal of Limnology and Freshwater Fisheries Research , 7 (2) , 101-107 . DOI: 10.17216/limnofish.827718
MLA Kayış, Ş. , Soyköse, G. , İpek, Z. Z. , Er, A. "Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey" . Journal of Limnology and Freshwater Fisheries Research 7 (2021 ): 101-107 <http://www.limnofish.org/en/pub/issue/64322/827718>
Chicago Kayış, Ş. , Soyköse, G. , İpek, Z. Z. , Er, A. "Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey". Journal of Limnology and Freshwater Fisheries Research 7 (2021 ): 101-107
RIS TY - JOUR T1 - Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey AU - Şevki Kayış , Gökhan Soyköse , Zeynep Zehra İpek , Akif Er Y1 - 2021 PY - 2021 N1 - doi: 10.17216/limnofish.827718 DO - 10.17216/limnofish.827718 T2 - Journal of Limnology and Freshwater Fisheries Research JF - Journal JO - JOR SP - 101 EP - 107 VL - 7 IS - 2 SN - -2149-4428 M3 - doi: 10.17216/limnofish.827718 UR - https://doi.org/10.17216/limnofish.827718 Y2 - 2020 ER -
EndNote %0 Journal of Limnology and Freshwater Fisheries Research Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey %A Şevki Kayış , Gökhan Soyköse , Zeynep Zehra İpek , Akif Er %T Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey %D 2021 %J Journal of Limnology and Freshwater Fisheries Research %P -2149-4428 %V 7 %N 2 %R doi: 10.17216/limnofish.827718 %U 10.17216/limnofish.827718
ISNAD Kayış, Şevki , Soyköse, Gökhan , İpek, Zeynep Zehra , Er, Akif . "Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey". Journal of Limnology and Freshwater Fisheries Research 7 / 2 (August 2021): 101-107 . https://doi.org/10.17216/limnofish.827718
AMA Kayış Ş. , Soyköse G. , İpek Z. Z. , Er A. Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey. Journal of Limnology and Freshwater Fisheries Research. 2021; 7(2): 101-107.
Vancouver Kayış Ş. , Soyköse G. , İpek Z. Z. , Er A. Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey. Journal of Limnology and Freshwater Fisheries Research. 2021; 7(2): 101-107.
IEEE Ş. Kayış , G. Soyköse , Z. Z. İpek and A. Er , "Determination of Bacterial Contamination and Antibiotic Resistance of the Bacteria in the Some Trout Farm Hatcheries in the Eastern Black Sea Region of Turkey", Journal of Limnology and Freshwater Fisheries Research, vol. 7, no. 2, pp. 101-107, Aug. 2021, doi:10.17216/limnofish.827718

Cited By

Antibiotic Resistance in Black Sea Microbial Communities
Frontiers in Environmental Science
https://doi.org/10.3389/fenvs.2022.823172