Effects of Different Protein Sources on Proteolotic Activity in Juveniles of Sterlet, Acipenser ruthenus Linnaeus 1758
Abstract
In this trial, Sterlet
sturgeon, Acipenser ruthenus, larvae
were fed by a standart feeding protocol from hatcing until 29th day,
and the proteolytic activity and the inhibition effect of different portein
sources on protease activity were also measured. The data obtained from study
showed that using soybean protein consantrate (38.57±10.44%) and corn gluten
(43.07±1.72%) as protein source in mixed feeds of dulcite juvenile strains
compared to other feed raw materials, was found to have a more positive effect
on proteolytic enzyme activity in terms of statistics (P<0.05). When the
binary combinations of feedstuffs were examined, he lowest inhibition rate was obtained using
1: 1 (47.93 ± 1.72%) fish meal: soy protein concentrate, and this value was
similar to that of soybean protein
consantrate and corn gluten as a single protien sources in the diets
(P>0.05). The highest inhibitory effect was obtained by using 3: 1 ratio of
wheat and brass bran (82.39±7.23%).
References
- Alarcon FJ, Moyano FJ, Diaz M, Fernandez-Diaz C, Yufera M. 1999. Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquac Nutr. 5(2):107-113. doi: 10.1046/j.1365-2095.1999.00093.x
- Alptekin C, Gökçek K. 2016. Balık yemlerinde kullanılan farklı protein kaynaklarının Sibirya mersini, Acipenser baerii Brandt 1869, juvenillerinin proteaz aktivitesi üzerine etkileri. Yunus Araştırma Bülteni. 16(3):201-207. [in Turkish] doi: 10.17683/yunus.32866
- Babaei SS, Kenari AA, Nazari R, Gisbert E. 2011. Developmental changes of digestive enzymes in Persian Sturgeon (Acipenser percius) during larval ontogeny. Aquaculture. 318(1-2):138-144. doi: 10.1016/j.aquaculture.2011.04.032
- Bradford MM. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dry binding. Anal Biochem. 72:248-254.
- Furne M, Hidalgo MC, Lopez A, Garcia-Gallego M, Morales AE, Domezain A, Domezaine J, Sanz A. 2005. Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss. A comperative study. Aquaculture. 250(1-2):391-398. doi: 10.1016/j.aquaculture.2005.05.017
- Garcia-Carreno FL. 1996. Proteinase inhibitors. Trends Food Sci Technol. 7(6):197-204. doi: 10.1016/0924-2244(96)10023-6
- Gawlicka A, Herold MA, Barrows FT, de le Noue J, Hung SSO. 2002. Effect of dieatary lipids on growth, fatty acid composition, intestinal absorbtion and hepatic storage in white sturgeon (Acipenser transmontanus) larvae. J Appl Icthyol. 18(4-6):673-681. doi: 10.1046/j.1439-0426.2002.00371.x
- Gökçek K, Szabo T, Alptekin C, Kurt R, Töre Y, Urbanyi B. 2016. The effect of feeding regime and inhibitory of different feed ingredients on proteases of Russian sturgeon, Acipenser gueldenstaedtii Brandt&Ratzenburg, 1833, at early life stages. Turkish J of Fisheries and Aquatic Sciences. 16(4): 1025-1029. doi: 10.4194/1303-2712-v16_4_29
- Kotzamanis YP, Vatsos IN, Cremer M, Illia V, Pyrenis G, Zonaras V, Buentello A, Van Eys J, 2012. Effects of high dietary inclusion of soybean meal and Navita 3010 soy supplemented with taurine on growth performance and muscle composition of European seabass (Dicentrarchus labrax). HCMR, Greece. (Technical Report)
- Napora-Rutkowski L, Kamaszewski M, Bielawski W, Ostaszewska T, Wegner A. 2009. Effects of starter diets on pancreatic enzyme activity in juvenile starlet (Acipenser ruthenus). The Israeli J Aquaculture-Bamidgeh, 61 (2): 143-150.
- Noori F, Gilbert VS, Sorgeloos P. 2012. Preliminary study on the activity of protease enzymes in Persian sturgeon (Acipenser persicus Borodin, 1897) larvae in response to different diets: effects on growth and survival. Aquac Res. 43(2):198-207. doi: 10.1111/j.1365-2109.2011.02816.x
- Sanz A, Llorente JL, Furne M, Ostos-Garrido V, Carmona R, Domezain A, Hidalgo MC. 2011. Digestive enzymes during ontogeny of the sturgeon Acipenser naccarii: intestine and pancreas development. J Appl Ichthyol. 27(5):1139-1146. doi: 10.1111/j.1439-0426.2011.01864.x
- Töre Y, Gökçek K, Alptekin C, Szabo T, Urbanyi B. 2014. Turna, Esox lucius Linneaus 1758, balığı larvalarının proteaz aktivitesi üzerine ticari yem hammaddelerinin inhibisyon etkilerinin tespiti. Doğu Anadolu 5. Su Ürünleri Sempozyumu; Elazığ, Türkiye.
- Walter HE. 1984. Proteinases: methods with hemoglobin, casein and azocoll as sub-strates, In: Methods of Enzymatic Analysis, Vol. 5. Weinheim: Verlag Chemie Press. s. 270-277.
- Willot P, Sabeau L, Gessner J, Arlati G, Bronzi P, Gulyas T, Berni P. 2001. Sturgeon farming in Western Europe: recent developments and perspectives. Aquat Living Resourc. 14(6):367-374. doi: 10.1016/S0990-7440(01)01136-6
Farklı Protein Kaynaklarının Çuka Mersini, Acipenser ruthenus Linnaeus 1758, Juvenillerinde Proteaz Aktivitesi Üzerine Etkileri
Abstract
Bu çalışmada, çuka balığı, Acipenser
ruthenus, larvaları yumurtadan çıkış
anından itibaren 29 gün boyunca standart besleme protokolü ile beslenmiştir.
Karma yeme geçiş yapılan 29. gündeki proteolitik aktivite ve farklı protein
kaynaklarının proteaz aktivite üzerine inhibisyon etkileri ölçülmüştür. Çalışmadan
elde edilen bilgiler ışığında, çuka balığı juvenillerinin karma yemlerinde
protein kaynağı olarak soya protein konsantresi (%38,57±10,44) ve mısır gluten (%43,07±1,72) kullanmanın, diğer yem hammaddeleri ile
karşılaştırıldığında, proteolitik enzim aktivitesini istatistiki açıdan daha
olumlu etki yaptığı görülmüştür (P<0,05). Yem hammaddelerinin ikili
kombinasyonları incelendiğinde ise, en düşük inhibisyon oranı balık unu: soya
protein konsantresinin 1:1 oranda kullanılması (%47,93±1,72) ile elde edilmiş
ve bu değerin soya protein konsantresi ve mısır gluteninin tek başlarına
kullanılması ile elde edilen etki ile aynı seviyede olduğu tespit edilmiştir
(P>0,05). En yüksek inhibe edici etki ise, buğday ile pirinç kepeğinin 3:1
oranda kullanılması ile oluşmuştur (%82,39±7,23).
References
- Alarcon FJ, Moyano FJ, Diaz M, Fernandez-Diaz C, Yufera M. 1999. Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquac Nutr. 5(2):107-113. doi: 10.1046/j.1365-2095.1999.00093.x
- Alptekin C, Gökçek K. 2016. Balık yemlerinde kullanılan farklı protein kaynaklarının Sibirya mersini, Acipenser baerii Brandt 1869, juvenillerinin proteaz aktivitesi üzerine etkileri. Yunus Araştırma Bülteni. 16(3):201-207. [in Turkish] doi: 10.17683/yunus.32866
- Babaei SS, Kenari AA, Nazari R, Gisbert E. 2011. Developmental changes of digestive enzymes in Persian Sturgeon (Acipenser percius) during larval ontogeny. Aquaculture. 318(1-2):138-144. doi: 10.1016/j.aquaculture.2011.04.032
- Bradford MM. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dry binding. Anal Biochem. 72:248-254.
- Furne M, Hidalgo MC, Lopez A, Garcia-Gallego M, Morales AE, Domezain A, Domezaine J, Sanz A. 2005. Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss. A comperative study. Aquaculture. 250(1-2):391-398. doi: 10.1016/j.aquaculture.2005.05.017
- Garcia-Carreno FL. 1996. Proteinase inhibitors. Trends Food Sci Technol. 7(6):197-204. doi: 10.1016/0924-2244(96)10023-6
- Gawlicka A, Herold MA, Barrows FT, de le Noue J, Hung SSO. 2002. Effect of dieatary lipids on growth, fatty acid composition, intestinal absorbtion and hepatic storage in white sturgeon (Acipenser transmontanus) larvae. J Appl Icthyol. 18(4-6):673-681. doi: 10.1046/j.1439-0426.2002.00371.x
- Gökçek K, Szabo T, Alptekin C, Kurt R, Töre Y, Urbanyi B. 2016. The effect of feeding regime and inhibitory of different feed ingredients on proteases of Russian sturgeon, Acipenser gueldenstaedtii Brandt&Ratzenburg, 1833, at early life stages. Turkish J of Fisheries and Aquatic Sciences. 16(4): 1025-1029. doi: 10.4194/1303-2712-v16_4_29
- Kotzamanis YP, Vatsos IN, Cremer M, Illia V, Pyrenis G, Zonaras V, Buentello A, Van Eys J, 2012. Effects of high dietary inclusion of soybean meal and Navita 3010 soy supplemented with taurine on growth performance and muscle composition of European seabass (Dicentrarchus labrax). HCMR, Greece. (Technical Report)
- Napora-Rutkowski L, Kamaszewski M, Bielawski W, Ostaszewska T, Wegner A. 2009. Effects of starter diets on pancreatic enzyme activity in juvenile starlet (Acipenser ruthenus). The Israeli J Aquaculture-Bamidgeh, 61 (2): 143-150.
- Noori F, Gilbert VS, Sorgeloos P. 2012. Preliminary study on the activity of protease enzymes in Persian sturgeon (Acipenser persicus Borodin, 1897) larvae in response to different diets: effects on growth and survival. Aquac Res. 43(2):198-207. doi: 10.1111/j.1365-2109.2011.02816.x
- Sanz A, Llorente JL, Furne M, Ostos-Garrido V, Carmona R, Domezain A, Hidalgo MC. 2011. Digestive enzymes during ontogeny of the sturgeon Acipenser naccarii: intestine and pancreas development. J Appl Ichthyol. 27(5):1139-1146. doi: 10.1111/j.1439-0426.2011.01864.x
- Töre Y, Gökçek K, Alptekin C, Szabo T, Urbanyi B. 2014. Turna, Esox lucius Linneaus 1758, balığı larvalarının proteaz aktivitesi üzerine ticari yem hammaddelerinin inhibisyon etkilerinin tespiti. Doğu Anadolu 5. Su Ürünleri Sempozyumu; Elazığ, Türkiye.
- Walter HE. 1984. Proteinases: methods with hemoglobin, casein and azocoll as sub-strates, In: Methods of Enzymatic Analysis, Vol. 5. Weinheim: Verlag Chemie Press. s. 270-277.
- Willot P, Sabeau L, Gessner J, Arlati G, Bronzi P, Gulyas T, Berni P. 2001. Sturgeon farming in Western Europe: recent developments and perspectives. Aquat Living Resourc. 14(6):367-374. doi: 10.1016/S0990-7440(01)01136-6
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | April 27, 2018 |
| Published in Issue | Year 2018Volume: 4 Issue: 1 |