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Farklı Öldürme Tekniklerinin Kahverengi Alabalık (Salmo trutta fario) Kan Biyokimyası Üzerine Etkileri

Year 2020, Volume: 6 Issue: 1, 25 - 29, 25.04.2020

Muhammed Atamanalp Murat Genç Gonca Alak , Nilüfer Sabuncuoğlu Esat Mahmut Kocaman Ömer Çoban Asuman Özkan Ziya Gökalp Ceylan

https://doi.org/10.17216/limnofish.522776

Abstract

Bu çalışmada, kahverengi alabalıklara (Salmo trutta fario) farklı öldürme teknikleri uygulanarak balıkların fizyolojik yanıtları bazı kan biyokimya enzimleri ile araştırılmıştır. Buzlu su, elektroşok, CO2, hipoksi ve kafasına vurma gibi farklı öldürme teknikleri, 75 balık kullanılarak denenmiş ve balıkların kan biyokimya parametrelerindeki değişim gözlemlenmiştir. Bu değişimlerde alkalin fosfataz (ALP) ve amilaz (AMS) istatistiki olarak önemsiz (p<0,05), kreatin kitinaz (CK) ve CK izoenzimlerinden olan CK-MB enzimi p<0,05 seviyesinde, alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), gama glutamil transferaz (GGT) ve laktat dehidrogenaz (LDH) ise p<0,05’ de önemli bulunmuştur. Sonuçlar, öldürme tekniklerinin kahverengi alabalıklarda stres kaynağı olabileceğini ve fizyolojik durumlarında etkili olduğunu göstermektedir. Çalışma bulguları, daha kısa sürede sonuçlanan öldürme tekniklerinin hayvan refahı açısından daha uygun olduğunu göstermiştir.

Keywords

Kahverengi alabalık biyokimya kan öldürme teknikleri

References

  • Alak G, Erdogan O, Ciltas A. 2010a. Hsp-70 gene expression analyses in the different ages of rainbow trout. Kafkas Univ Vet Fak Derg. 16(Suppl-B):183-187. doi:10.9775/kvfd.2009.757
  • Alak G, Hisar SA, Hisar O, Kaban G, Kaya M. 2010b. Microbiological and chemical properties of bonito fish (Sarda sarda) fillets packaged with chitosan film, modified atmosphere and vacuum. Kafkas Univ Vet Fak Derg. 16 (Suppl-A):S73-S80. doi:10.9775/kvfd.2009.1475
  • Atamanalp M, Kocaman EM, Ucar A, Alak G. 2012. Kadmiyum toksisitesine karşı humik asitin koruyucu etkisinin kahverengi alabalıklarda (Salmo trutta fario) araştırılması. Fen Edebiyat Dergisi. 14(1):405-415.
  • Boissy A. 1995. Fear and fearfulness in animals. Q Rev of Biol. 70(2):165–191. doi:10.1086/418981
  • Bórnez R, Linares MB, Vergara H. 2009. Systems stunning with CO2 gas on Manchego light lambs: Physiologic responses and stunning effectiveness. Meat Sci. 82(1):133–138. doi: 10.1016/j.meatsci.2009.01.003
  • Botsoglou E, Govaris A, Ambrosiadis L, Fletouris D, Botsoglou N. 2014. Effect of leaf (Olea europen L.) extracts on protein and lipid oxidation of long-term frozen n-3 fatty acids-enriched pork patties. Meat Sci. 98: 150-157. doi:10.1016/j.meatsci.2014.05.015
  • Bricknell IR, Bowden TJ, Bruno DW, MacLachlan P, Johnstone R, Ellis AE. 1999. Susceptibility of atlantic halibut, Hippoglossus hippoglossus (L) to infection with typical and atypical Aeromonas salmonicida. Aquaculture. 175(1):1–13. doi: 10.1016/S0044-8486(99)00025-3
  • Chen L, Li C, Ullah N, Guo Y, Sun X, Wang X, Feng X. 2016. Different physicochemical, structural and digestibility characteristics of myofibrillar protein from PSE and normal pork before and after oxidation. Meat Sci. 121: 228–237. doi: 10.1016/j.meatsci.2016.06.010
  • Conte, F.S. 2004. Stress and the welfare of cultured fish. Appl Anim Behav. Sci. 86 (3-4): 205–223. doi: 10.1016/j.applanim.2004.02.003
  • Cui X, Xiong YL, Kong B, Zhao X, Liu N. 2012. Hydroxyl radical-stressed whey protein isolate: Chemical and structural properties. Food and Bioprocess Techn. 5(6): 2454–2461. doi: 10.1007/s11947-011-0515-9
  • El-Khaldi ATF. 2010. Effect of different stress factors on some physiological parameters of Nile tilapia (Oreochromis niloticus). Saudi J Biol Sci. 17(3):241–246. doi: 10.1016/j.sjbs.2010.04.009
  • Fırat O, Inandı AŞ. 2016. Biochemical toxicity of mercury and protective effect of zeolite on this toxicity in Oreochromis niloticus. TrJFAS. 31(2):86–95. doi: 10.18864/TJAS201610
  • Gencer YG, Cinar A, Comba B. 2015. Stresin ratlarda bazı karaciğer enzimleri (AST, ALT, ALP) üzerine etkilerinin araştırılması. Atatürk Üniversitesi Vet Bil Derg. 10(1):21-26.
  • Hilmy AM, Shabana MB, Daabes AY. 1985. Effects of cadmium toxicity upon the in vivo and in vitro activity of proteins and five enzymes in blood serum and tissue homogenates of Mugil cephalus. Comp Biochem Physiol Part C. 81(1): 145-153. doi: 10.1016/0742-8413(85)90106-9
  • Kayhan FE, Muslu MN, Koc ND. 2009. Stress and biological responses seen in aquatic organisms due to some trace elements. J Fisheries Sci. 3(2):153-162. doi: 10.3153/jfscom.2009019
  • Kıran ÖE, Comlekcioglu U, Dostbil N. 2006. Some microbial enzymes and usage fields in the industry. KSU J Sci Engin. 9(1):12-19.
  • Mert N. 1996. Veteriner Klinik Biyokimya. Bursa: Uludağ Üniversitesi Veteriner Fakültesi Yayını Ders Kitabı 288s.
  • Mishima T, Nonaka T, Okamoto A, Tsuchimoto M, Ishiya T, Tachibana K, Tsuchimoto M. 2005. Influence of storage temperatures and killing procedures on postmortem changes in the muscle of horse mackerel caught near Nagasaki Prefecture, Japan. Fisheries Sci. 71(1):187-194. doi:10.1111/j.1444-2906.2005.00947.x
  • Nakyinsige K, Man YB, Aghwan ZA, Zulkifli I, Goh YM, Abu Bakar F, Al-Kahtani HA, Sazili AQ. 2013.Stunning and animal welfare from Islamic and scientific perspectives. Meat Sci. 95(2): 352-61. doi: 10.1016/j.meatsci.2013.04.006
  • Özbek H, Cengiz N, Him A, Uğraş S, Özgökçe F, Erdoğan E. 2006.Yüksek kolesterollü diyetle beslenen sıçanlarda Thymus fallax F. (kekik) yapraklarının kan kolesterol seviyesi üzerine etkisi. Van Tıp Derg. 13 (3): 71-77.
  • Roth B, Moeller D, Veland JO, Imsland A, Slınde A. 2002. The effect of stunning methods on rigor mortis and texture properties of atlantic salmon (Salmo salar). J Food Sci. 67(4): 1462-1466. doi: 10.1111/j.1365-2621.2002.tb10306.x
  • Sabow AB, Gohb YM, Zulkifli I, Sazili AQ, Kaka U, Ab Kadi MZA, Ebrahimi M. 2016. Blood parameters and electroencephalographic responses of goats to slaughter without stunning. Meat Sci. 121: 148–155. doi: 10.1016/j.meatsci.2016.05.009
  • Svete AN, Čebulj-Kadunc N, Frangez R, Kruljcteve P. 2012. Serum cortisol and haematological, biochemical and antioxidant enzyme variables in horse blood sampled in a slaughterhouse lairage, immediately before stunning and during exsanguination. Animal. 6(8):1300–1306. doi:10.1017/S1751731112000079
  • Tekeli SK, Ormen A, Mengi A. 1996.Safkan arap ve ingiliz taylarında serum AST, ALT ve ALP aktiviteleri üzerinde çalışmalar. İstanbul Üni Vet Fak Derg. 22(1):127-133. Urbieta C, Gines R. 2000. Optimisation of slaughtering method in gilthead sea bream (Sparus aurata) industrial application in fish farms. Global quality assessment in Mediteranean aquaculture, Zaragosa. CHIEAM-IAAZ, Spain.
  • Utrera M, Estevez M. 2012. Oxidation of myofibrillar proteins and impaired functionality: Underlying mechanisms of the carbonylation pathway. J Agr Food Chem. 60(32): 8002–8011. doi: 10.1021/jf302111j
  • Venturini FP, Vargas Baldi SC, Paris G, Costa TD, Rucin Que DS, Melo MP, Macedo Viegas EM. 2018. Effects of different stunning methods on blood markers and enzymatic activity of stress responses of tilapia (Oreochromis niloticus). Ital J Anim Sci. 17(4): 1094-1098. doi: 10.1080/1828051X.2018.1426396
  • Vijayan MM, Pereira C, Grau EG, Iwama GK. 1997. Metabolic responses associated with confinement stress in Tilapia: the role of cortisol. Comp Biochem Phys C. 116 (1): 89-85. doi: 10.1016/S0742-8413(96)00124-7
  • Werner M, Gallo C. 2008. Effects of transport, lairage and stunning on the concentrations of some blood constituents in horses destined for slaughter. Livest Sci. 115: 94–98. doi: 10.1016/j.livsci.2007.12.023
  • Winther Christensen J, Kelling LJ, Lindestrøm Nielsen B. 2005. Responses of horses to novel visual, olfactory and auditory stimuli. Appl Anim Behav Sci. 93(1-2):53–65. doi: 10.1016/j.applanim.2005.06.017
  • Witeska M, Baka I. 2002. The effect of long-term cadmium exposure on common carp blood. Fresen Environ Bull. 11(12A): 1059-1065.
  • Zampacavallo G, Parisi G, Mecatti M, Lupi P, Giorgi G, Poli BM. 2015. Evaluation of different methods of stunning/killing sea bass (Dicentrarchus labrax) by tissue stress/quality indicators. J Food Sci Technol. 52(5): 2585–2597. doi: 10.1007/s13197-014-1324-8
  • Zhang L, Li Q, Lyu J, Kong C, Song S, Luo Y. 2017. The impact of stunning methods on stress conditions and quality of silver carp (Hypophthalmichthys molitrix) fillets stored at 4 degrees C during 72 h postmortem. Food Chem. 216:130–137. doi: 10.1016/j.foodchem.2016.08.004

The Effects of Different Stunning Techniques on Blood Biochemistry of Brown Trout (Salmo trutta fario)

Year 2020, Volume: 6 Issue: 1, 25 - 29, 25.04.2020

Muhammed Atamanalp Murat Genç Gonca Alak , Nilüfer Sabuncuoğlu Esat Mahmut Kocaman Ömer Çoban Asuman Özkan Ziya Gökalp Ceylan

https://doi.org/10.17216/limnofish.522776

Abstract

In this study, physiological responses of brook trout (Salmo trutta fario) were researched after the different stunning methods with biochemical enzymes. Icy water, electroshock, CO2, hypoxia, and head hitting were tried as stunning techniques to 75 fish and the alterations in the blood biochemistry parameters were analyzed. Alkaline phosphates (ALP), and amylase (AMS) were found as not statistically important, but alanine transaminases (ALT), aspartate transaminases (AST), creatin chitinase (CK), CK-MB (one of the CK isoenzymes), gamma glutamil transferase (GGT) and lactate dehydrogenase (LDH) levels were important (p<0.05). According to these results, the different stunning techniques affected the stress levels and physiological situations of brook trout. The study findings showed that killing techniques resulting in a shorter period were more suitable for animal welfare.

Keywords

Brook trout biochemistry blood stunning methods

References

  • Alak G, Erdogan O, Ciltas A. 2010a. Hsp-70 gene expression analyses in the different ages of rainbow trout. Kafkas Univ Vet Fak Derg. 16(Suppl-B):183-187. doi:10.9775/kvfd.2009.757
  • Alak G, Hisar SA, Hisar O, Kaban G, Kaya M. 2010b. Microbiological and chemical properties of bonito fish (Sarda sarda) fillets packaged with chitosan film, modified atmosphere and vacuum. Kafkas Univ Vet Fak Derg. 16 (Suppl-A):S73-S80. doi:10.9775/kvfd.2009.1475
  • Atamanalp M, Kocaman EM, Ucar A, Alak G. 2012. Kadmiyum toksisitesine karşı humik asitin koruyucu etkisinin kahverengi alabalıklarda (Salmo trutta fario) araştırılması. Fen Edebiyat Dergisi. 14(1):405-415.
  • Boissy A. 1995. Fear and fearfulness in animals. Q Rev of Biol. 70(2):165–191. doi:10.1086/418981
  • Bórnez R, Linares MB, Vergara H. 2009. Systems stunning with CO2 gas on Manchego light lambs: Physiologic responses and stunning effectiveness. Meat Sci. 82(1):133–138. doi: 10.1016/j.meatsci.2009.01.003
  • Botsoglou E, Govaris A, Ambrosiadis L, Fletouris D, Botsoglou N. 2014. Effect of leaf (Olea europen L.) extracts on protein and lipid oxidation of long-term frozen n-3 fatty acids-enriched pork patties. Meat Sci. 98: 150-157. doi:10.1016/j.meatsci.2014.05.015
  • Bricknell IR, Bowden TJ, Bruno DW, MacLachlan P, Johnstone R, Ellis AE. 1999. Susceptibility of atlantic halibut, Hippoglossus hippoglossus (L) to infection with typical and atypical Aeromonas salmonicida. Aquaculture. 175(1):1–13. doi: 10.1016/S0044-8486(99)00025-3
  • Chen L, Li C, Ullah N, Guo Y, Sun X, Wang X, Feng X. 2016. Different physicochemical, structural and digestibility characteristics of myofibrillar protein from PSE and normal pork before and after oxidation. Meat Sci. 121: 228–237. doi: 10.1016/j.meatsci.2016.06.010
  • Conte, F.S. 2004. Stress and the welfare of cultured fish. Appl Anim Behav. Sci. 86 (3-4): 205–223. doi: 10.1016/j.applanim.2004.02.003
  • Cui X, Xiong YL, Kong B, Zhao X, Liu N. 2012. Hydroxyl radical-stressed whey protein isolate: Chemical and structural properties. Food and Bioprocess Techn. 5(6): 2454–2461. doi: 10.1007/s11947-011-0515-9
  • El-Khaldi ATF. 2010. Effect of different stress factors on some physiological parameters of Nile tilapia (Oreochromis niloticus). Saudi J Biol Sci. 17(3):241–246. doi: 10.1016/j.sjbs.2010.04.009
  • Fırat O, Inandı AŞ. 2016. Biochemical toxicity of mercury and protective effect of zeolite on this toxicity in Oreochromis niloticus. TrJFAS. 31(2):86–95. doi: 10.18864/TJAS201610
  • Gencer YG, Cinar A, Comba B. 2015. Stresin ratlarda bazı karaciğer enzimleri (AST, ALT, ALP) üzerine etkilerinin araştırılması. Atatürk Üniversitesi Vet Bil Derg. 10(1):21-26.
  • Hilmy AM, Shabana MB, Daabes AY. 1985. Effects of cadmium toxicity upon the in vivo and in vitro activity of proteins and five enzymes in blood serum and tissue homogenates of Mugil cephalus. Comp Biochem Physiol Part C. 81(1): 145-153. doi: 10.1016/0742-8413(85)90106-9
  • Kayhan FE, Muslu MN, Koc ND. 2009. Stress and biological responses seen in aquatic organisms due to some trace elements. J Fisheries Sci. 3(2):153-162. doi: 10.3153/jfscom.2009019
  • Kıran ÖE, Comlekcioglu U, Dostbil N. 2006. Some microbial enzymes and usage fields in the industry. KSU J Sci Engin. 9(1):12-19.
  • Mert N. 1996. Veteriner Klinik Biyokimya. Bursa: Uludağ Üniversitesi Veteriner Fakültesi Yayını Ders Kitabı 288s.
  • Mishima T, Nonaka T, Okamoto A, Tsuchimoto M, Ishiya T, Tachibana K, Tsuchimoto M. 2005. Influence of storage temperatures and killing procedures on postmortem changes in the muscle of horse mackerel caught near Nagasaki Prefecture, Japan. Fisheries Sci. 71(1):187-194. doi:10.1111/j.1444-2906.2005.00947.x
  • Nakyinsige K, Man YB, Aghwan ZA, Zulkifli I, Goh YM, Abu Bakar F, Al-Kahtani HA, Sazili AQ. 2013.Stunning and animal welfare from Islamic and scientific perspectives. Meat Sci. 95(2): 352-61. doi: 10.1016/j.meatsci.2013.04.006
  • Özbek H, Cengiz N, Him A, Uğraş S, Özgökçe F, Erdoğan E. 2006.Yüksek kolesterollü diyetle beslenen sıçanlarda Thymus fallax F. (kekik) yapraklarının kan kolesterol seviyesi üzerine etkisi. Van Tıp Derg. 13 (3): 71-77.
  • Roth B, Moeller D, Veland JO, Imsland A, Slınde A. 2002. The effect of stunning methods on rigor mortis and texture properties of atlantic salmon (Salmo salar). J Food Sci. 67(4): 1462-1466. doi: 10.1111/j.1365-2621.2002.tb10306.x
  • Sabow AB, Gohb YM, Zulkifli I, Sazili AQ, Kaka U, Ab Kadi MZA, Ebrahimi M. 2016. Blood parameters and electroencephalographic responses of goats to slaughter without stunning. Meat Sci. 121: 148–155. doi: 10.1016/j.meatsci.2016.05.009
  • Svete AN, Čebulj-Kadunc N, Frangez R, Kruljcteve P. 2012. Serum cortisol and haematological, biochemical and antioxidant enzyme variables in horse blood sampled in a slaughterhouse lairage, immediately before stunning and during exsanguination. Animal. 6(8):1300–1306. doi:10.1017/S1751731112000079
  • Tekeli SK, Ormen A, Mengi A. 1996.Safkan arap ve ingiliz taylarında serum AST, ALT ve ALP aktiviteleri üzerinde çalışmalar. İstanbul Üni Vet Fak Derg. 22(1):127-133. Urbieta C, Gines R. 2000. Optimisation of slaughtering method in gilthead sea bream (Sparus aurata) industrial application in fish farms. Global quality assessment in Mediteranean aquaculture, Zaragosa. CHIEAM-IAAZ, Spain.
  • Utrera M, Estevez M. 2012. Oxidation of myofibrillar proteins and impaired functionality: Underlying mechanisms of the carbonylation pathway. J Agr Food Chem. 60(32): 8002–8011. doi: 10.1021/jf302111j
  • Venturini FP, Vargas Baldi SC, Paris G, Costa TD, Rucin Que DS, Melo MP, Macedo Viegas EM. 2018. Effects of different stunning methods on blood markers and enzymatic activity of stress responses of tilapia (Oreochromis niloticus). Ital J Anim Sci. 17(4): 1094-1098. doi: 10.1080/1828051X.2018.1426396
  • Vijayan MM, Pereira C, Grau EG, Iwama GK. 1997. Metabolic responses associated with confinement stress in Tilapia: the role of cortisol. Comp Biochem Phys C. 116 (1): 89-85. doi: 10.1016/S0742-8413(96)00124-7
  • Werner M, Gallo C. 2008. Effects of transport, lairage and stunning on the concentrations of some blood constituents in horses destined for slaughter. Livest Sci. 115: 94–98. doi: 10.1016/j.livsci.2007.12.023
  • Winther Christensen J, Kelling LJ, Lindestrøm Nielsen B. 2005. Responses of horses to novel visual, olfactory and auditory stimuli. Appl Anim Behav Sci. 93(1-2):53–65. doi: 10.1016/j.applanim.2005.06.017
  • Witeska M, Baka I. 2002. The effect of long-term cadmium exposure on common carp blood. Fresen Environ Bull. 11(12A): 1059-1065.
  • Zampacavallo G, Parisi G, Mecatti M, Lupi P, Giorgi G, Poli BM. 2015. Evaluation of different methods of stunning/killing sea bass (Dicentrarchus labrax) by tissue stress/quality indicators. J Food Sci Technol. 52(5): 2585–2597. doi: 10.1007/s13197-014-1324-8
  • Zhang L, Li Q, Lyu J, Kong C, Song S, Luo Y. 2017. The impact of stunning methods on stress conditions and quality of silver carp (Hypophthalmichthys molitrix) fillets stored at 4 degrees C during 72 h postmortem. Food Chem. 216:130–137. doi: 10.1016/j.foodchem.2016.08.004
There are 32 citations in total.

Details

Primary Language English
Subjects Limnology
Journal Section Research Article
Authors

Muhammed Atamanalp 0000-0002-2038-3921

Murat Genç 0000-0002-9565-0887

Gonca Alak 0000-0002-7539-1152

Nilüfer Sabuncuoğlu 0000-0002-9350-814X

Esat Mahmut Kocaman 0000-0002-6426-7260

Ömer Çoban 0000-0003-2368-6247

Asuman Özkan 0000-0001-8857-3409

Ziya Gökalp Ceylan 0000-0001-8644-5905

Publication Date April 25, 2020
Published in Issue Year 2020 Volume: 6 Issue: 1

Cite

APA Atamanalp, M., Genç, M., Alak, G., … Sabuncuoğlu, N. (2020). The Effects of Different Stunning Techniques on Blood Biochemistry of Brown Trout (Salmo trutta fario). Journal of Limnology and Freshwater Fisheries Research, 6(1), 25-29. https://doi.org/10.17216/limnofish.522776