Araştırma Makalesi
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Assessment of Chromogenic Media in Bacterial Fish Pathogens

Yıl 2021, Cilt 7, Sayı 2, 150 - 156, 26.08.2021
https://doi.org/10.17216/limnofish.810225

Öz

Accurate and quick identification of bacterial fish disease has great importance for effective disease control and prevent economic loss in aquaculture facilities. Generally used agar media allow the growth of wide-range bacteria but new chromogenic media results in colored colonies that lead to presumptive identification without requiring biochemical or serological tests. In this study, the recently commercialized chromogenic media; chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart and chromID® Staphylococcus aureus Elite (BioMérieux, France) were tested with growth performance and colony coloration of bacterial fish pathogens. chromID® CPS® Elite and chromID® CPS® Elite / Columbia CNA +5% sheep blood agar were detected the most suitable culture media to grow for all five isolated fish pathogens (i.e., Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri and Vibrio anguillarum). Of which, L. garvieae was the only one observed with green colonies. chromID® Vibrio designed for V. parahaemolyticus and V cholerae was lead dark pink/purple colonies by V. anguillarum. The chromID® MRSA Smart and chromID® S. aureus Elite were detected not effective for incubation of bacterial fish pathogens.

Kaynakça

  • Algöet M, Bayley AE, Roberts EG, Feist SW, Wheeler RW, Verner‐Jeffreys DW. 2009. Susceptibility of selected freshwater fish species to a UK Lactococcus garvieae isolate. J Fish Dis. 32(10):825-834. doi: 10.1111/j.1365-2761.2009.01058.x
  • Alrabadi NI. 2012. The effect of several antibiotics on Lactococcus garvieae isolated from Jordanian dairy products. Am J Agric Biol Sci. 7(4):468-472. doi: 10.3844/ajabssp.2012.468.472
  • Austin B, Austin DA. 2007. Bacterial Fish Pathogens 4th ed. the UK: Springer 552 p.
  • Balta F, Dengiz Balta Z. 2019. The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia. J Anatolian Env and Anim Sciences. 4(1):27-33. doi: 10.35229/jaes.527258
  • Blanco-Abad V, Ansede-Bermejo J, Rodrıguez-Castro A, Martinez-Urtaza J. 2009. Evaluation of different procedures for the optimized detection of Vibrio parahaemolyticus in mussels and environmental samples. Int J Food Microbiol. 129(3):229-236. doi: 10.1016/j.ijfoodmicro.2008.11.028
  • Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A, Buschmann AH. 2013. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol. 15(7):1917-1942. doi: 10.1111/1462-2920.12134
  • Eddabra R, Piemont Y, Scheftel JM. 2011. Evaluation of a new chromogenic medium, chromID™ Vibrio, for the isolation and presumptive identification of Vibrio cholerae and Vibrio parahaemolyticus from human clinical specimens. Eur J Clin Microbiol. 30(6):733-737. doi: 10.1007/s10096-010-1145-2
  • Farmer JJ, Hickman-Brenner FW, Kelly MT. 1985. Vibrio. In: Lennette EH, Balows A, Hausler Jr WJ, Shadomy HJ, editors. Manual of Clinical Microbiology, 4th ed. Washington DC: American Society for Microbiology Press. p. 282-301.
  • Ghittino C, Prearo M. 1992. Report of Streptococcosis in rainbow trout (Oncorhynchus mykiss) in Italy: preliminary note. Boll Soc Ital Patol. 8:4-9.
  • Gil P, Vivas J, Gallardo CS, Rodriguez LA. 2000. First isolation of Staphylococcus warneri, from diseased rainbow trout, Oncorhynchus mykiss (Walbaum), in Northwest Spain. J Fish Dis. 23(4):295-298. doi: 10.1046/j.1365-2761.2000.00244.x
  • Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: a review. Estuaries. 14(4):345-360. doi: 10.2307/1352260
  • Kayış S, Çapkın E, Balta F, Altınok I. 2009. Bacteria in rainbow trout (Oncorhynchus mykiss) in the Southern Black Sea Region of Turkey - A survey. Isr J Aquacult-Bamid.61(4):339-344. doi: 10.46989/001c.20564
  • Muziasari WI, Managaki S, Pärnänen K, Karkman A, Lyra C, Tamminen M, Suzuki S, Virta M. 2014. Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment. PlosOne.9(3):e92702. doi:10.1371/journal.pone.0092702
  • Perry JD, Freydière AM. 2007. The application of chromogenic media in clinical microbiology. J Appl Microbiol. 103(6):2046-2055. doi: 10.1111/j.1365-2672.2007.03442.x
  • Perry JD. 2017. A decade of development of chromogenic culture media for clinical microbiology in an era of molecular diagnostics. Clin Microbiol Rev. 30(2):449-479. doi: 10.1128/CMR.00097-16
  • Pinto A, Terio V, Novello L, Tantillo G. 2011. Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus. Food Control. 22(1):124-127. doi: 10.1016/j.foodcont.2010.06.013
  • Shotts EB, Teska JD. 1989. Bacterial pathogens of aquatic vertebrates. In: Austin B, Austin DA, editors. Methods for the Microbiological Examinationof Fish and Shellfish. Oxford: Ellis Horwood Ltd. p. 164-181.
  • Tanrıkul TT. 2012. Efficacy of a whole cell Lactococcus garvieae vaccine in rainbow trout (Oncorhynchus mykiss). J Anim Vet Adv. 11(7):886-889. doi: 10.3923/javaa.2012.886.889
  • Ture M, Boran H. 2015. Phenotypic and genotypic antimicrobial resistance of Lactococcus sp. strains isolated from rainbow trout (Oncorhynchus mykiss). Bull Vet Inst Pulawy. 59(1):37-42. doi: 10.1515/bvip-2015-0006
  • Yarbrough ML, Wallace MA, Marshall C, Mathias E, Burnham CAD. 2016. Culture of urine specimens using chromID CPS elite medium can expedite Escherichia coli identification and reduce hands-on time in the clinical laboratory. J Clin Microbiol. 54(11):2767-2773. doi: 10.1128/JCM.01376-16

Bakteriyel Balık Patojenleri için Kromojenik Besiyerlerinin Değerlendirilmesi

Yıl 2021, Cilt 7, Sayı 2, 150 - 156, 26.08.2021
https://doi.org/10.17216/limnofish.810225

Öz

Akuakültür işletmelerinde etkin hastalık kontrolü ve ekonomik kayıpların önlenmesi için bakteriyel balık hastalıklarının doğru ve hızlı tanımlanması büyük önem taşımaktadır. Genel olarak kullanılan agar besiyerlerinde çok çeşitli bakteri türünün üremesi gözlenirken, yeni kromojenik besiyerleri, biyokimyasal ve serolojik test gerektirmeden renkli koloni görünümüyle tahmini ön tanımlama imkânı sunmaktadır. Bu çalışmada yakın zamanda ticarileşen kromojenik besiyerlerinde (chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart ve chromID® Staphylococcus aureus Elite (BioMérieux, France)) bakteriyel balık patojenlerinin üremesi ve koloni renklenmeleri tespit edilmiştir. ChromID® CPS® Elite ve chromID® CPS® Elite / Columbia CNA +5% sheep blood agarın test edilen 5 farklı bakteriyel balık patojeni (Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri ve Vibrio anguillarum) için en uygun besiyeri olduğu, ancak sadece L. garvieae’nin yeşil koloni görünümü ile ürediği belirlenmiştir. Vibrio parahaemolyticus ve Vibrio cholerae’nın hızlı teşhisi için geliştirilen chromID® Vibrio besiyerinde V. anguillarum kolonileri koyu pembe/mor renkli olarak gözlenmiş, chromID® MRSA Smart ve chromID® S. aureus Elite besiyerlerinin bakteriyel balık patojenlerinin üremesi için uygun olmadığı belirlenmiştir.

Kaynakça

  • Algöet M, Bayley AE, Roberts EG, Feist SW, Wheeler RW, Verner‐Jeffreys DW. 2009. Susceptibility of selected freshwater fish species to a UK Lactococcus garvieae isolate. J Fish Dis. 32(10):825-834. doi: 10.1111/j.1365-2761.2009.01058.x
  • Alrabadi NI. 2012. The effect of several antibiotics on Lactococcus garvieae isolated from Jordanian dairy products. Am J Agric Biol Sci. 7(4):468-472. doi: 10.3844/ajabssp.2012.468.472
  • Austin B, Austin DA. 2007. Bacterial Fish Pathogens 4th ed. the UK: Springer 552 p.
  • Balta F, Dengiz Balta Z. 2019. The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia. J Anatolian Env and Anim Sciences. 4(1):27-33. doi: 10.35229/jaes.527258
  • Blanco-Abad V, Ansede-Bermejo J, Rodrıguez-Castro A, Martinez-Urtaza J. 2009. Evaluation of different procedures for the optimized detection of Vibrio parahaemolyticus in mussels and environmental samples. Int J Food Microbiol. 129(3):229-236. doi: 10.1016/j.ijfoodmicro.2008.11.028
  • Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A, Buschmann AH. 2013. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol. 15(7):1917-1942. doi: 10.1111/1462-2920.12134
  • Eddabra R, Piemont Y, Scheftel JM. 2011. Evaluation of a new chromogenic medium, chromID™ Vibrio, for the isolation and presumptive identification of Vibrio cholerae and Vibrio parahaemolyticus from human clinical specimens. Eur J Clin Microbiol. 30(6):733-737. doi: 10.1007/s10096-010-1145-2
  • Farmer JJ, Hickman-Brenner FW, Kelly MT. 1985. Vibrio. In: Lennette EH, Balows A, Hausler Jr WJ, Shadomy HJ, editors. Manual of Clinical Microbiology, 4th ed. Washington DC: American Society for Microbiology Press. p. 282-301.
  • Ghittino C, Prearo M. 1992. Report of Streptococcosis in rainbow trout (Oncorhynchus mykiss) in Italy: preliminary note. Boll Soc Ital Patol. 8:4-9.
  • Gil P, Vivas J, Gallardo CS, Rodriguez LA. 2000. First isolation of Staphylococcus warneri, from diseased rainbow trout, Oncorhynchus mykiss (Walbaum), in Northwest Spain. J Fish Dis. 23(4):295-298. doi: 10.1046/j.1365-2761.2000.00244.x
  • Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: a review. Estuaries. 14(4):345-360. doi: 10.2307/1352260
  • Kayış S, Çapkın E, Balta F, Altınok I. 2009. Bacteria in rainbow trout (Oncorhynchus mykiss) in the Southern Black Sea Region of Turkey - A survey. Isr J Aquacult-Bamid.61(4):339-344. doi: 10.46989/001c.20564
  • Muziasari WI, Managaki S, Pärnänen K, Karkman A, Lyra C, Tamminen M, Suzuki S, Virta M. 2014. Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment. PlosOne.9(3):e92702. doi:10.1371/journal.pone.0092702
  • Perry JD, Freydière AM. 2007. The application of chromogenic media in clinical microbiology. J Appl Microbiol. 103(6):2046-2055. doi: 10.1111/j.1365-2672.2007.03442.x
  • Perry JD. 2017. A decade of development of chromogenic culture media for clinical microbiology in an era of molecular diagnostics. Clin Microbiol Rev. 30(2):449-479. doi: 10.1128/CMR.00097-16
  • Pinto A, Terio V, Novello L, Tantillo G. 2011. Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus. Food Control. 22(1):124-127. doi: 10.1016/j.foodcont.2010.06.013
  • Shotts EB, Teska JD. 1989. Bacterial pathogens of aquatic vertebrates. In: Austin B, Austin DA, editors. Methods for the Microbiological Examinationof Fish and Shellfish. Oxford: Ellis Horwood Ltd. p. 164-181.
  • Tanrıkul TT. 2012. Efficacy of a whole cell Lactococcus garvieae vaccine in rainbow trout (Oncorhynchus mykiss). J Anim Vet Adv. 11(7):886-889. doi: 10.3923/javaa.2012.886.889
  • Ture M, Boran H. 2015. Phenotypic and genotypic antimicrobial resistance of Lactococcus sp. strains isolated from rainbow trout (Oncorhynchus mykiss). Bull Vet Inst Pulawy. 59(1):37-42. doi: 10.1515/bvip-2015-0006
  • Yarbrough ML, Wallace MA, Marshall C, Mathias E, Burnham CAD. 2016. Culture of urine specimens using chromID CPS elite medium can expedite Escherichia coli identification and reduce hands-on time in the clinical laboratory. J Clin Microbiol. 54(11):2767-2773. doi: 10.1128/JCM.01376-16

Ayrıntılar

Birincil Dil İngilizce
Konular Fen
Yayınlanma Tarihi August 2021
Bölüm Araştırma Makalesi
Yazarlar

Prof.dr. T. Tansel TANRIKUL
İZMİR KATİP ÇELEBİ ÜNİVERSİTESİ
0000-0002-1684-7965
Türkiye


Ezgi DİNÇTÜRK (Sorumlu Yazar)
IZMIR KATIP CELEBI UNIVERSITY
0000-0002-5826-2383
Türkiye

Destekleyen Kurum Izmir Katip Celebi University Scientific Research Projects Coordinatorship
Proje Numarası 2017-GAP-SUÜF-0005
Yayımlanma Tarihi 26 Ağustos 2021
Yayınlandığı Sayı Yıl 2021, Cilt 7, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { limnofish810225, journal = {Journal of Limnology and Freshwater Fisheries Research}, issn = {}, eissn = {2149-4428}, address = {}, publisher = {Eğirdir Su Ürünleri Araştırma Enstitü Müdürlüğü}, year = {2021}, volume = {7}, pages = {150 - 156}, doi = {10.17216/limnofish.810225}, title = {Assessment of Chromogenic Media in Bacterial Fish Pathogens}, key = {cite}, author = {Tanrıkul, Prof.dr. T. Tansel and Dinçtürk, Ezgi} }
APA Tanrıkul, P. T. T. & Dinçtürk, E. (2021). Assessment of Chromogenic Media in Bacterial Fish Pathogens . Journal of Limnology and Freshwater Fisheries Research , 7 (2) , 150-156 . DOI: 10.17216/limnofish.810225
MLA Tanrıkul, P. T. T. , Dinçtürk, E. "Assessment of Chromogenic Media in Bacterial Fish Pathogens" . Journal of Limnology and Freshwater Fisheries Research 7 (2021 ): 150-156 <http://www.limnofish.org/tr/pub/issue/64322/810225>
Chicago Tanrıkul, P. T. T. , Dinçtürk, E. "Assessment of Chromogenic Media in Bacterial Fish Pathogens". Journal of Limnology and Freshwater Fisheries Research 7 (2021 ): 150-156
RIS TY - JOUR T1 - Assessment of Chromogenic Media in Bacterial Fish Pathogens AU - Prof.dr. T. Tansel Tanrıkul , Ezgi Dinçtürk Y1 - 2021 PY - 2021 N1 - doi: 10.17216/limnofish.810225 DO - 10.17216/limnofish.810225 T2 - Journal of Limnology and Freshwater Fisheries Research JF - Journal JO - JOR SP - 150 EP - 156 VL - 7 IS - 2 SN - -2149-4428 M3 - doi: 10.17216/limnofish.810225 UR - https://doi.org/10.17216/limnofish.810225 Y2 - 2020 ER -
EndNote %0 Journal of Limnology and Freshwater Fisheries Research Assessment of Chromogenic Media in Bacterial Fish Pathogens %A Prof.dr. T. Tansel Tanrıkul , Ezgi Dinçtürk %T Assessment of Chromogenic Media in Bacterial Fish Pathogens %D 2021 %J Journal of Limnology and Freshwater Fisheries Research %P -2149-4428 %V 7 %N 2 %R doi: 10.17216/limnofish.810225 %U 10.17216/limnofish.810225
ISNAD Tanrıkul, Prof.dr. T. Tansel , Dinçtürk, Ezgi . "Assessment of Chromogenic Media in Bacterial Fish Pathogens". Journal of Limnology and Freshwater Fisheries Research 7 / 2 (Ağustos 2021): 150-156 . https://doi.org/10.17216/limnofish.810225
AMA Tanrıkul P. T. T. , Dinçtürk E. Assessment of Chromogenic Media in Bacterial Fish Pathogens. Journal of Limnology and Freshwater Fisheries Research. 2021; 7(2): 150-156.
Vancouver Tanrıkul P. T. T. , Dinçtürk E. Assessment of Chromogenic Media in Bacterial Fish Pathogens. Journal of Limnology and Freshwater Fisheries Research. 2021; 7(2): 150-156.
IEEE P. T. T. Tanrıkul ve E. Dinçtürk , "Assessment of Chromogenic Media in Bacterial Fish Pathogens", Journal of Limnology and Freshwater Fisheries Research, c. 7, sayı. 2, ss. 150-156, Ağu. 2021, doi:10.17216/limnofish.810225