Rheum Ribes L. Ekstratını İçeren Sert Jelatin Kapsüllerin Kalite Kontrolünün Yapılması ve Oksidatif Stres Üzerine Etkilerinin İncelenmesi
Abstract
ÖZ
Rheum türleri tıbbi öneme sahiptir. Rheum
ribes L. yerel olarak ışkın, uçkun ve uşgun olarak bilinmektedir. Genellikle
Lübnan’da, İran’da ve Türkiye’nin doğusunda yetişmektedir. Rheum ribes L.
bitkisinin çeşitli bölümlerinden elde edilmiş ekstraktlar birçok hastalığın
tedavisinde kullanılmaktadır. Bu çalışmada, Rheum ribes L. ekstraktının
hazırlanması, elde edilen toz ekstraktlarının Avrupa Farmakopesi’ne uygun
olarak toz kontrollerinin yapılması, hazırlanan ekstraktın sert jelatin kapsül
formülasyonunun elde edilmesi ve Rheum ribes L. ektstraktını içeren sert
jelatin kapsüllerin kapsül kalite kontrol çalışmalarının yapılması, bitkisel
ekstraktın in vitro olarak antioksidan özellik gösterip göstermediği
incelenmiştir. Sert jelatin kapsüllere dolumu gerçekleştirilen Rheum ribes
L. ekstraktlarında yığın dansitesi, sıkıştırılmış dansite, sıkıştırılabilirlik
indeksi, hausner oranı, akış süresi, yığın açısı tayini, elek analizi; dolumu
yapılan sert jelatin kapsüllere ise dağılma testi, ağırlık sapması testi yapılmıştır.
Bitki ekstraktının antioksidan aktivitesi DPPH metodu ile ölçülmüştür. Elde
edilen sonuçlara göre yığın açısı 26,66
± 0.33 ̊ yığın dansitesi 0,37±0,06 g/ml, sıkıştırılmış dansite 0,46±0,09
g/ml, elek analizinin sonucuna göre d50: 214,734±12,36 µm, d90:
43,99±2,92 µm bulunmuş olup, yapılan dağılma testinde ise suni mide vasatında 1
dakika 12 saniyede, suni barsak vasatında 2 dakika 10 saniyede kapsüller
dağılma göstermiştir, dolumları yapılan kapsüllerde ağırlık sapması 250,16±1.85 mg’dır. Rheum ribes
L. ekstraktının IC50’si 74,1±4,8
µg /mL olarak bulunmuştur. Elde edilen verilere göre, Avrupa Farmakopesi
baz alındığında kapsüllere dolum yapılan tozun akışkanlığı mükemmel, elek
analizi sonucuna göre tozun inceliği çok kaba toz olarak bulunmuştur. Bu
sonuçlara göre; Rheum ribes L. ekstraktı içeren sert jelatin kapsüllerin
formülasyon açısından bir sorun teşkil etmediği ve piyasada gıda takviyesi
olarak kullanılabileceği düşünülmektedir.
Anahtar Kelimeler: Rheum ribes L., sert jelatin kapsül, oksidatif stres,
kalite kontrol, Işkın
Supporting Institution
BAP
Project Number
2016-2-TP2-1937
Thanks
Mersin Üniversitesi Bilimsel Araştırmalar Birimine Teşekkür ederiz.
References
- 1. Alaadin, A.M., Al-Khateeb, E.H., Jager, A.K., Antibacterial activity of the Iraqi Rheum ribes root, Pharmaceutical biology, 2007, 45, 688-690.
- 2. Naqishbandi, A.M., Josefsen, K., Pedersen, M.E., Jager, A.K., Hypoglycemic activity of Iraqi Rheum ribes root extract, Pharmaceutical biology, 2009, 47, 380-383.
- 3. Ebrahimi, F., Fatahi, Z., Jabbari, F., Aboali, L., Antibacterial Effect of Rheum ribes on EscherichiaColi, Iran J PublicHealth, 2014, 43, 282-282.
- 4. Ozbek, H., Ceylan, E., Kara, M., Ozgokce, F., Koyuncu, M., Hypoglycemic effect of Rheum ribes roots in alloxan induced diabetic and normal mice, Scand J Lab Anim Sci, 2004, 31, 113-115.
- 5. The european pharmacopoeia and common european standards for drugs and medicaments, Journal of the Association of Official Analytical Chemists, 1968, 51, 89-96.
- 6. Kedare, S.B., Singh, R.P., Genesis and development of DPPH method of antioxidant assay, J Food Sci Tech Mys, 2011, 48, 412-422.
- 7. Weigert, G.G., Ineu, A.P., Gomes, P., Evaluation of Hard Gelatin Capsules and Hydroxypropyl Methylcellulose Containing Ampicillin, Quim Nova,2012, 35, 286-290.
- 8. The United States Pharmacopeia. The National Formulary. Rockville, Md. United States Pharmacopeial Convention, 1979.
- 9. Couto, R.O., Martins, F.S., Chaul, L.T., Conceicao, E.C., Freitas, L.A.P., Bara, M.T.F., Paula, J.R., Spraydrying of Eugeniadysenterica extract: effects of in-process parameters on product quality, RevBras Farmacogn,2013, 23, 115-123.
- 10. Gallo, L., Ramirez-Rigo, M.V., Pina, J., Bucala, V., A comparative study of spray-dried medicinal plant aqueous extracts. Drying performance and product quality, ChemEng Res Des, 2015, 104, 681-694.
- 11. Almukainzi, M., Salehi, M., Bou-Chacra, N.A., Lobenberg, R., Investigation of thePerformance of theDisintegration Test forDietarySupplements, Aaps J, 2010, 12, 602-607.
- 12. Sato-Masumoto, N., Masada, S., Takahashi, S., Terasaki, S., Yokota, Y., Hakamatsuka, T., Goda, Y., Disintegration Test of Health Food Products Containing Ginkgo Biloba L. Or VitexAgnus-Castus L. in theJapaneseMarket, Medicines, 2015, 2, 47-54.
- 13. Öztürk, M., Aydoğmuş-Öztürk, F., Duru, M. E., Topçu, G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food chemistry, 2007, 103(2), 623-630.
- 14. Hasan, S.M.R., Hossain, M.M., Akter, R., Jamila, M., Mazumder, M.E.H., Rahman, S., DPPH free radical scavenging activity of some Bangladeshi medicinal plants, JMedPlantsRes,2009, 3, 875-879.
Quality Control of Hard Gelatin Capsules Containing Rheum Ribes L. Extract and Investigation of Its Effects on Oxidative Stress
Abstract
ABSTRACT
Rheum species are important plants in terms of medicine. Rheum ribes L. is locally known as ışkın, uçkun and uşgun. It is usually grown in Lebanon, Iran and the east of Turkey. Extracts from various parts of the plant Rheum ribes L. are used in the treatment of many diseases .In this study, the preparation of Rheum ribes L. extract, powder controls of the obtained extracts in accordance with the European Pharmacopoeia, obtaining hard gelatin capsule formulation of prepared extract and capsule quality control studies of hard gelatin capsules including Rheum ribes L. extract, it was investigated whether the herbal extract demonstrated antioxidant properties in vitro. Hard gelatin capsules filled with Rheum ribes L. extracts were subjected to angle of repose determination, bulk density, tapped density, sieve analysis, compressibility index, hausner ratio, flow time and filled hard gelatin capsules were subjected to disintegration test and a weight deviation test. Antioxidant activity of plant extract was calculated by DPPH method. According to the results obtained, the angle of repose was 26,66 ± 0.33 ̊, the bulk density was 0,37±0,06 g/ml, the tapped density was 0,46±0,09 g/ml, the particle size as d50: 214,734±12,36 µm, d90: 43,99±2,92 μm was exhibited by sieve analysis and the disintegration test presented disintegration in artificial gastric medium at the end of 1 minute 12 seconds , in artificial instestinal medium at the end of 2 minutes and 10 seconds and the weight deviation in filled capsules was 250,16±1.85 mg. The IC50 of Rheum ribes L. extract was found to be 74.1 ± 4.8 µg / mL. According to the data obtained, based on the European Pharmacopoeia, the flowability of the powder filled into capsules was ‘excellent’ and the particle size of the powder was found to be ‘very coarse’ powder according to sieve analysis. Hereunder to these results; it is believed that hard gelatin capsules containing Rheum ribes L. extract are not a problem in formulation and can be used as a food supplement in the market.
Project Number
2016-2-TP2-1937
References
- 1. Alaadin, A.M., Al-Khateeb, E.H., Jager, A.K., Antibacterial activity of the Iraqi Rheum ribes root, Pharmaceutical biology, 2007, 45, 688-690.
- 2. Naqishbandi, A.M., Josefsen, K., Pedersen, M.E., Jager, A.K., Hypoglycemic activity of Iraqi Rheum ribes root extract, Pharmaceutical biology, 2009, 47, 380-383.
- 3. Ebrahimi, F., Fatahi, Z., Jabbari, F., Aboali, L., Antibacterial Effect of Rheum ribes on EscherichiaColi, Iran J PublicHealth, 2014, 43, 282-282.
- 4. Ozbek, H., Ceylan, E., Kara, M., Ozgokce, F., Koyuncu, M., Hypoglycemic effect of Rheum ribes roots in alloxan induced diabetic and normal mice, Scand J Lab Anim Sci, 2004, 31, 113-115.
- 5. The european pharmacopoeia and common european standards for drugs and medicaments, Journal of the Association of Official Analytical Chemists, 1968, 51, 89-96.
- 6. Kedare, S.B., Singh, R.P., Genesis and development of DPPH method of antioxidant assay, J Food Sci Tech Mys, 2011, 48, 412-422.
- 7. Weigert, G.G., Ineu, A.P., Gomes, P., Evaluation of Hard Gelatin Capsules and Hydroxypropyl Methylcellulose Containing Ampicillin, Quim Nova,2012, 35, 286-290.
- 8. The United States Pharmacopeia. The National Formulary. Rockville, Md. United States Pharmacopeial Convention, 1979.
- 9. Couto, R.O., Martins, F.S., Chaul, L.T., Conceicao, E.C., Freitas, L.A.P., Bara, M.T.F., Paula, J.R., Spraydrying of Eugeniadysenterica extract: effects of in-process parameters on product quality, RevBras Farmacogn,2013, 23, 115-123.
- 10. Gallo, L., Ramirez-Rigo, M.V., Pina, J., Bucala, V., A comparative study of spray-dried medicinal plant aqueous extracts. Drying performance and product quality, ChemEng Res Des, 2015, 104, 681-694.
- 11. Almukainzi, M., Salehi, M., Bou-Chacra, N.A., Lobenberg, R., Investigation of thePerformance of theDisintegration Test forDietarySupplements, Aaps J, 2010, 12, 602-607.
- 12. Sato-Masumoto, N., Masada, S., Takahashi, S., Terasaki, S., Yokota, Y., Hakamatsuka, T., Goda, Y., Disintegration Test of Health Food Products Containing Ginkgo Biloba L. Or VitexAgnus-Castus L. in theJapaneseMarket, Medicines, 2015, 2, 47-54.
- 13. Öztürk, M., Aydoğmuş-Öztürk, F., Duru, M. E., Topçu, G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food chemistry, 2007, 103(2), 623-630.
- 14. Hasan, S.M.R., Hossain, M.M., Akter, R., Jamila, M., Mazumder, M.E.H., Rahman, S., DPPH free radical scavenging activity of some Bangladeshi medicinal plants, JMedPlantsRes,2009, 3, 875-879.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Article |
| Authors | |
| Project Number | 2016-2-TP2-1937 |
| Publication Date | January 31, 2020 |
| Submission Date | September 27, 2019 |
| Published in Issue | Year 2020 Volume: 10 Issue: 1 |
Cite
Cited By
Işgın (Rheum ribes L.) Bitkisinin Önemi, Kullanım Alanları ve Sorunları
Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi
https://doi.org/10.31020/mutftd.1280588
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Periodical scientific publication of Mersin University School of Medicine. Can not be cited without reference. Responsibility of the articles belong to the authors
Ayşegül Tuğuz
from composition of İlter Uzel named “Dioscorides and his Student
Address
Mersin Üniversitesi Tıp Fakültesi Tıp Tarihi ve Etik Anabilim Dalı Çiftlikköy Kampüsü
Yenişehir / Mersin