Cryopreservation of sperm cells
is an essential process applied for long-term conservation of aquatic genetic
resources. The goal of this research was to determine effect different of straws volumes and thawing rates on the post-thaw
quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen
was cryopreserved according to conventional slow freezing protocol. For this
aim, the cryosolution
contained 75 mM NaCl,
70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8)
supplemented with 10% MeOH. Following equilibration at +4°C for 10 min, semen was
packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for
10 min at -120ºC) and finally stored in liquid nitrogen (-196ºC) tank. Thawing of cryopreserved semen process
was performed at 30ºC for 10, 20 and 30 seconds in a water bath. Fertilization
was performed using ratio of 1x105 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved
sperm packed in 1.5 mL straws that thawed at 30ºC for 30 s. According to the
results of this research, sperm cryopreserved with ionic extender containing
10% methanol and packed in 1.5 mL straws are suitable to achieve high
fertilization of common carp eggs.
Journal Section | Research Article |
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Authors | |
Publication Date | April 24, 2017 |
Published in Issue | Year 2017Volume: 3 Issue: 1 |