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Koi (Cyprinus carpio) ve Japon Balıklarında (Carassius auratus) Betanodavirus Varlığının Araştırılması

Year 2023, , 37 - 42, 28.04.2023

Ufuk Oğuz , Murat Kaplan , Gülnur Kalaycı

https://doi.org/10.17216/limnofish.1141342
Cited By: 1

Abstract

Nodaviridae familyasında yer alan betanodavirus’un neden olduğu Viral nervöz nekrozis (VNN) ya da bir diğer adıyla Viral ensefalopati-retinopati (VER) dünyanın pek çok yerinde hem deniz balıklarında hem de tatlı su balıklarında görülür. Larval ve yavru boylarda, nadiren de yetişkin balıklarda hastalığa neden olmaktadır. Özellikle yetiştiriciliği yapılan balık türlerinde önemli ekonomik kayıplara neden olduğu için son yıllarda enfeksiyonla ilgili araştırmalara odaklanılmıştır. Bu çalışmada, Antalya bölgesinde yetiştiricilik faaliyeti gösteren akvaryum işletmelerinde Koi ve Japon balığı türlerinde betanodavirus varlığı araştırılmıştır. Üç farklı akvaryum işletmesinden her bir türü temsilen en az 30’ar adet olmak üzere toplam 180 balık örneklendi ve Real Time RT-PCR (RT-qPRC) metodu ile test edildi. Çalışma sonunda hiçbir örnekte betanodavirus RNA’sına rastlanmadı. Bu çalışma ile Türkiye’de koi ve japon balığı türlerinde betanodavirus varlığı moleküler tekniklerle ilk defa araştırılmıştır.

Keywords

Betanodavirus Japon balığı Koi Real Time RT-PCR (RT-qPCR) Türkiye

Supporting Institution

Akdeniz Su Ürünleri Araştırma Üretme ve Eğitim Enstitüsü

Thanks

Araştırma çalışması kapsamında numune temin edilecek işletmelerin seçilmesi ve numune alma döneminde katkılarından dolayı enstitümüz çalışanları Su Ürünleri Yüksek Mühendisi Sn. Soner Sezen'e, numunelerin hazırlanması ve laboratuvar uygulamaları esnasında yardımını esirgemeyen Su Ürünleri Yüksek Mühendisi Sn. Meltem BUĞDAYCI KANLI arkadaşlarıma teşekkür ederim.

References

  • Anonim, 2019. Viral encephalopathy and retinopathy. OIE - Manual of Diagnostic Tests for Aquatic Animals Chapter 2.3.12.
  • Athanassopoulou F, Billinis C, Prapas T. 2004. Important disease conditions of newly cultured species in intensive freshwater farms in Greece: first incidence of nodavirus infection in Acipenser sp. Dis. Aquat. Org. 60(3), 247-252. doi:10.3354/dao060247 Bandín I, Souto S, 2020. Betanodavirus and VER Disease: A 30-year Research Review. Pathogens.9(2):106. doi:10.3390/pathogens9020106 Bigarré L, Cabon J, Baud M, Heimann M, Body A, Lieffrig F, Castric J. 2009. Outbreak of betanodavirus infection in tilapia, Oreochromis niloticus (L.), in fresh water. J Fish Dis. 32(8), 667-673. doi: 10.1111/j.1365-2761.2009.01037.x.
  • Bovo G, Gustinelli A, Quaglio F, Gobbo F, Panzarin V, Fusaro A, Mutinelli F, Caffara M, Fioravanti ML. 2011. Viral encephalopathy and retinopathy outbreak in freshwater fish farmed in İtaly. Dis. Aquat. Org., 96,45-54. https://doi.org/10.3354/dao02367
  • Chi SC, Shieh JR, Lin SJ. 2003. Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Dis. Aquat. Org. 55(3), 221-228. doi:10.3354/dao055221
  • Dalla Valle L, Zanella L, Patarnello P, Paolucci L, Belvedere P, Colombo L. 2000. Development of a sensitive diagnostic assay for fish nervous necrosis virus based on RT-PCR plus nested PCR. J Fish Dis. 23, 321-327. doi: 10.1046/j.1365-2761.2000.00255.x.
  • Furusawa R, Okinaka Y, Nakai T. 2006. Betanodavirus infection in the freshwater model fish medaka (Oryzias latipes). J Gen Virol. 87(8), 2333-2339. doi: 10.1099/vir.0.81761-0.
  • Furusawa R, Okinaka Y, Uematsu K, Nakai T. 2007. Screening of freshwater fish species for their susceptibility to a betanodavirus. Dis. Aquat. Org. 77,119-125. doi: 10.3354/dao01841.
  • Gomez DK, Lim DJ, Baeck GW, Youn HJ, Shin NS, Youn HY, Hwang CY, Park JH, Park SC. 2006. Detection of betanodaviruses in apparently healthy aquarium fishes and invertabrates. J Vet Sci. 7(4), 369-374. doi: 10.4142/jvs.2006.7.4.369.
  • Hegde A, The HC, Lam TJ, Sin YM. 2003. Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata – comparative characterization and pathogenicity studies. Arch Virol. 148: 575-586. Iwamoto T, Nakai T, Mori K, Arimoto M, Furusawa I. 2000. Cloning of the fish cell line SSN-1 for piscine nodaviruses. Dis. Aquat. Org. 43, 81–89. doi:10.3354/dao043081.
  • Jithendran KP, Shekhar MS, Kannappan S, Azad IS. 2011. Nodavirus infection in freshwater ornamental fishes in India: diagnostic histopathology and nested RT-PCR. Asian Fisheries Science (24) :12-19.
  • Kalaycı G, Özkan B, Pekmez K, Kaplan M. 2016. Levrek ve Çipura kuluçkahanelerinde Viral Nervöz Nekrozis Hastalığının Durumu. XII. Veteriner Hekimleri Mikrobiyoloji Kongresi Poster Sunumu. Kapadokya/Nevşehir, 30 Ağustos - 02 Eylül 2016. (in Turkish)
  • Kaplan M ve Karaoğlu MT. 2021. Investigation of betanodavirus in sea bass (Dicentrarchus labrax) at all production stages in all hatcheries and on selected farms in Turkey. Arch. Virol. 166(12), 3343-3356.
  • Kaplan M, Pekmez K, Özkan B, Çağırgan A. A, Kalaycı G, 2021. Detection of RGNNV genotype betanodavirus in the Black Sea and monitoring studies. Dis. Aquat. Org. 144, 117-121.
  • Kaplan M, Pekmez K, Cagirgan AA, Arslan F, Özkan B, Kalaycı G. 2022. The first detection of betanodavirus reassortant genotype (RGNNV/SJNNV) isolated from gilthead sea bream (Sparus aurata) in the Turkish coastlines: The importance of screening and monitoring studies for identifying the source of the infection. J Fish Dis.
  • Keawcharoen J, Techangamsuwan S, Ponpornpisit A, Lombardini ED, Patchimasiri T, Pirarat N. 2015. Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm‐raised tilapia Oreochromis niloticus (L.) in Thailand. J Fish Dis. 38(1), 49-54. doi: 10.1111/jfd.12200.
  • Kılıçerkan M ve Çek Ş. 2011. Hatay ilçelerindeki akvaryum işletmelerinin genel profili’nin çıkarılması üzerine bir araştırma. Journal of the Institute of Science and Technology, 1(4), 77-82. (in Turkish)
  • Maltese C ve Bovo G. 2007. Viral encephelopathy and retinopathy. İttiopatologia. 4: 93-147.
  • Mushiake K, Nishizawa T, Nkai T, Furusawa I, Muroga K. 1994. Control of VNN in Striped Jack: Selection of Spawners Based on the Detection of SJNNV Gene by Polymerase Chain Reaction (PCR). Fish Pathology, 29 (3) 177-182. doi: 10.3147/jsfp.29.177.
  • Nagai T, Nishizawa T. 1999. Sequence of the non-structural protein gene encoded by RNA1 of striped jack nervous necrosis virus. Journal of General Virology, 80(11), 3019-3022.
  • Nishizawa T, Furuhashi M, Nagai T, Nakai T, Muroga K. 1997. Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Applied and environmental microbiology, 63(4), 1633-1636.doi: 10.1128/aem.63.4.1633-1636.1997.
  • OIE 2019. Manual of Diagnostic Tests for Aquatic Animals. Section 2.3. Diseases of Fish. Chapter 2.3.12 Viral encephalopathy and retinopathy.
  • Özkan Özyer B, Kalaycı G, İnçoğlu Ș, Pekmez K, Küçükali Y. 2014. The first isolation of betanodavirus from cultured seabass in Turkey. Bornova Veteriner Bilimleri Dergisi, 36(50), 13-17.
  • Pala S ve Yılmaz E. 2020. Ordu İlinde Akvaryum Sektörünün Mevcut Durumu, Sorunları ve Çözüm Önerileri. Acta Aquatica Turcica, 16(3), 387-395.
  • Panzarin V, Patarnello P, Mori A, Rampazzo E, Cappellozza E, Bovo G, Cattoli G. 2010. Development and validation of a real-time Taqman PCR assay for the detection of betanodavirus in clinical specimens. Arch Virol. 155:1197-1203. doi: 10.1007/s00705-010-0701-5.
  • Panzarin V, Fusaro A, Monne I, Cappellozza E, Patarnello P, Bovo G, Cattoli G. 2012. Molecular epidemiology and evolutionary dynamics of betanodavirus in southern Europe. Infect Genet Evol. 12(1), 63-70. doi: 10.1016/j.meegid.2011.10.007.
  • Panzarin V, Cappellozza E, Mancin M, Milani A, Toffan A, Terregino C, Cattoli G. 2014. In vitro study of the replication capacity of the RGNNV and the SJNNV betanodavirus genotypes and their natural reassortants in response to temperature. Veterinary research, 45(1), 1-11.
  • Toffan A, Pascoli F, Pretto T, Panzarin V, Abbadi M, Buratin A, Padrós F. 2017. Viral nervous necrosis in gilthead sea bream (Sparus aurata) caused by reassortant betanodavirus RGNNV/SJNNV: An emerging threat for Mediterranean aquaculture. Scientific Reports, 7(1), 1-12. doi: 10.10387srep46755
  • Vázquez-Salgado L, Olveira JG, Dopazo CP, Bandín I. 2020. Role of rotifer (Brachionus plicatilis) and Artemia (Artemia salina) nauplii in the horizontal transmission of a natural nervous necrosis virus (NNV) reassortant strain to Senegalese sole (Solea senegalensis) larvae. Veterinary Quarterly, 40(1), 205-214. doi: 10.1080/01652176.2020.1810357.
  • Vendramin N, Patarnello P, Toffan A, Panzarin V, Cappellozza E, Tedesco P, Terlizzi A, Terregino C, Cattoli G. 2013. Viral retinopathy in groupers (Epinephelus spp.) in southern İtaly: a threat for wild endangered species? BMC Vet. Res. 9,20

Investigation of Betanodavirus Presence in Koi (Cyprinus carpio) and Goldfish (Carassius auratus)

Year 2023, , 37 - 42, 28.04.2023

Ufuk Oğuz , Murat Kaplan , Gülnur Kalaycı

https://doi.org/10.17216/limnofish.1141342
Cited By: 1

Abstract

Viral nervous necrosis (VNN), also known as Viral encephalopathy-retinopathy (VER), which is caused by betanodavirus in the nodaviridae family, is seen in both marine and freshwater fish in many parts of the world. It causes disease in larvae and juveniles, rarely in adult fish. Since it causes significant economic losses, in the recent years the research has been especially focused on the infections related to it. In this study, the presence of betanodavirus was investigated in Koi and goldfish species in aquarium facilities operating in the Antalya region. A total of 180 fish, at least 30 representing each species, were sampled from three different aquarium facilities and tested by the Real Time RT-PCR (RT-qPCR) method. At the end of the study, betanodavirus RNA was not found in any of the samples. With this study, the presence of betanodavirus in koi and goldfish species in Türkiye was investigated for the first time by the molecular techniques.

Keywords

Betanodavirus Goldfish Koi Real Time RT-PCR (RT-qPCR) Türkiye

References

  • Anonim, 2019. Viral encephalopathy and retinopathy. OIE - Manual of Diagnostic Tests for Aquatic Animals Chapter 2.3.12.
  • Athanassopoulou F, Billinis C, Prapas T. 2004. Important disease conditions of newly cultured species in intensive freshwater farms in Greece: first incidence of nodavirus infection in Acipenser sp. Dis. Aquat. Org. 60(3), 247-252. doi:10.3354/dao060247 Bandín I, Souto S, 2020. Betanodavirus and VER Disease: A 30-year Research Review. Pathogens.9(2):106. doi:10.3390/pathogens9020106 Bigarré L, Cabon J, Baud M, Heimann M, Body A, Lieffrig F, Castric J. 2009. Outbreak of betanodavirus infection in tilapia, Oreochromis niloticus (L.), in fresh water. J Fish Dis. 32(8), 667-673. doi: 10.1111/j.1365-2761.2009.01037.x.
  • Bovo G, Gustinelli A, Quaglio F, Gobbo F, Panzarin V, Fusaro A, Mutinelli F, Caffara M, Fioravanti ML. 2011. Viral encephalopathy and retinopathy outbreak in freshwater fish farmed in İtaly. Dis. Aquat. Org., 96,45-54. https://doi.org/10.3354/dao02367
  • Chi SC, Shieh JR, Lin SJ. 2003. Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Dis. Aquat. Org. 55(3), 221-228. doi:10.3354/dao055221
  • Dalla Valle L, Zanella L, Patarnello P, Paolucci L, Belvedere P, Colombo L. 2000. Development of a sensitive diagnostic assay for fish nervous necrosis virus based on RT-PCR plus nested PCR. J Fish Dis. 23, 321-327. doi: 10.1046/j.1365-2761.2000.00255.x.
  • Furusawa R, Okinaka Y, Nakai T. 2006. Betanodavirus infection in the freshwater model fish medaka (Oryzias latipes). J Gen Virol. 87(8), 2333-2339. doi: 10.1099/vir.0.81761-0.
  • Furusawa R, Okinaka Y, Uematsu K, Nakai T. 2007. Screening of freshwater fish species for their susceptibility to a betanodavirus. Dis. Aquat. Org. 77,119-125. doi: 10.3354/dao01841.
  • Gomez DK, Lim DJ, Baeck GW, Youn HJ, Shin NS, Youn HY, Hwang CY, Park JH, Park SC. 2006. Detection of betanodaviruses in apparently healthy aquarium fishes and invertabrates. J Vet Sci. 7(4), 369-374. doi: 10.4142/jvs.2006.7.4.369.
  • Hegde A, The HC, Lam TJ, Sin YM. 2003. Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata – comparative characterization and pathogenicity studies. Arch Virol. 148: 575-586. Iwamoto T, Nakai T, Mori K, Arimoto M, Furusawa I. 2000. Cloning of the fish cell line SSN-1 for piscine nodaviruses. Dis. Aquat. Org. 43, 81–89. doi:10.3354/dao043081.
  • Jithendran KP, Shekhar MS, Kannappan S, Azad IS. 2011. Nodavirus infection in freshwater ornamental fishes in India: diagnostic histopathology and nested RT-PCR. Asian Fisheries Science (24) :12-19.
  • Kalaycı G, Özkan B, Pekmez K, Kaplan M. 2016. Levrek ve Çipura kuluçkahanelerinde Viral Nervöz Nekrozis Hastalığının Durumu. XII. Veteriner Hekimleri Mikrobiyoloji Kongresi Poster Sunumu. Kapadokya/Nevşehir, 30 Ağustos - 02 Eylül 2016. (in Turkish)
  • Kaplan M ve Karaoğlu MT. 2021. Investigation of betanodavirus in sea bass (Dicentrarchus labrax) at all production stages in all hatcheries and on selected farms in Turkey. Arch. Virol. 166(12), 3343-3356.
  • Kaplan M, Pekmez K, Özkan B, Çağırgan A. A, Kalaycı G, 2021. Detection of RGNNV genotype betanodavirus in the Black Sea and monitoring studies. Dis. Aquat. Org. 144, 117-121.
  • Kaplan M, Pekmez K, Cagirgan AA, Arslan F, Özkan B, Kalaycı G. 2022. The first detection of betanodavirus reassortant genotype (RGNNV/SJNNV) isolated from gilthead sea bream (Sparus aurata) in the Turkish coastlines: The importance of screening and monitoring studies for identifying the source of the infection. J Fish Dis.
  • Keawcharoen J, Techangamsuwan S, Ponpornpisit A, Lombardini ED, Patchimasiri T, Pirarat N. 2015. Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm‐raised tilapia Oreochromis niloticus (L.) in Thailand. J Fish Dis. 38(1), 49-54. doi: 10.1111/jfd.12200.
  • Kılıçerkan M ve Çek Ş. 2011. Hatay ilçelerindeki akvaryum işletmelerinin genel profili’nin çıkarılması üzerine bir araştırma. Journal of the Institute of Science and Technology, 1(4), 77-82. (in Turkish)
  • Maltese C ve Bovo G. 2007. Viral encephelopathy and retinopathy. İttiopatologia. 4: 93-147.
  • Mushiake K, Nishizawa T, Nkai T, Furusawa I, Muroga K. 1994. Control of VNN in Striped Jack: Selection of Spawners Based on the Detection of SJNNV Gene by Polymerase Chain Reaction (PCR). Fish Pathology, 29 (3) 177-182. doi: 10.3147/jsfp.29.177.
  • Nagai T, Nishizawa T. 1999. Sequence of the non-structural protein gene encoded by RNA1 of striped jack nervous necrosis virus. Journal of General Virology, 80(11), 3019-3022.
  • Nishizawa T, Furuhashi M, Nagai T, Nakai T, Muroga K. 1997. Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Applied and environmental microbiology, 63(4), 1633-1636.doi: 10.1128/aem.63.4.1633-1636.1997.
  • OIE 2019. Manual of Diagnostic Tests for Aquatic Animals. Section 2.3. Diseases of Fish. Chapter 2.3.12 Viral encephalopathy and retinopathy.
  • Özkan Özyer B, Kalaycı G, İnçoğlu Ș, Pekmez K, Küçükali Y. 2014. The first isolation of betanodavirus from cultured seabass in Turkey. Bornova Veteriner Bilimleri Dergisi, 36(50), 13-17.
  • Pala S ve Yılmaz E. 2020. Ordu İlinde Akvaryum Sektörünün Mevcut Durumu, Sorunları ve Çözüm Önerileri. Acta Aquatica Turcica, 16(3), 387-395.
  • Panzarin V, Patarnello P, Mori A, Rampazzo E, Cappellozza E, Bovo G, Cattoli G. 2010. Development and validation of a real-time Taqman PCR assay for the detection of betanodavirus in clinical specimens. Arch Virol. 155:1197-1203. doi: 10.1007/s00705-010-0701-5.
  • Panzarin V, Fusaro A, Monne I, Cappellozza E, Patarnello P, Bovo G, Cattoli G. 2012. Molecular epidemiology and evolutionary dynamics of betanodavirus in southern Europe. Infect Genet Evol. 12(1), 63-70. doi: 10.1016/j.meegid.2011.10.007.
  • Panzarin V, Cappellozza E, Mancin M, Milani A, Toffan A, Terregino C, Cattoli G. 2014. In vitro study of the replication capacity of the RGNNV and the SJNNV betanodavirus genotypes and their natural reassortants in response to temperature. Veterinary research, 45(1), 1-11.
  • Toffan A, Pascoli F, Pretto T, Panzarin V, Abbadi M, Buratin A, Padrós F. 2017. Viral nervous necrosis in gilthead sea bream (Sparus aurata) caused by reassortant betanodavirus RGNNV/SJNNV: An emerging threat for Mediterranean aquaculture. Scientific Reports, 7(1), 1-12. doi: 10.10387srep46755
  • Vázquez-Salgado L, Olveira JG, Dopazo CP, Bandín I. 2020. Role of rotifer (Brachionus plicatilis) and Artemia (Artemia salina) nauplii in the horizontal transmission of a natural nervous necrosis virus (NNV) reassortant strain to Senegalese sole (Solea senegalensis) larvae. Veterinary Quarterly, 40(1), 205-214. doi: 10.1080/01652176.2020.1810357.
  • Vendramin N, Patarnello P, Toffan A, Panzarin V, Cappellozza E, Tedesco P, Terlizzi A, Terregino C, Cattoli G. 2013. Viral retinopathy in groupers (Epinephelus spp.) in southern İtaly: a threat for wild endangered species? BMC Vet. Res. 9,20
There are 29 citations in total.

Details

Primary Language Turkish
Subjects Limnology
Journal Section Research Article
Authors

Ufuk Oğuz 0000-0003-0918-9113

Murat Kaplan 0000-0002-2634-6478

Gülnur Kalaycı 0000-0002-2024-303X

Early Pub Date April 27, 2023
Publication Date April 28, 2023
Published in Issue Year 2023

Cite

APA Oğuz, U., Kaplan, M., & Kalaycı, G. (2023). Koi (Cyprinus carpio) ve Japon Balıklarında (Carassius auratus) Betanodavirus Varlığının Araştırılması. Journal of Limnology and Freshwater Fisheries Research, 9(1), 37-42. https://doi.org/10.17216/limnofish.1141342

Cited By

The Current Status of Viral Nervous Necrosis Disease in Türkiye
Journal of Limnology and Freshwater Fisheries Research
https://doi.org/10.17216/limnofish.1449488