Acid soluble collagens (ASC) from bone (ASC-B) and skin (ASC-S) of mangar (Luciobarbus esocinus (Heckel, 1843) were extracted, characterized, and their amino acid profiles were determined. To best of our knowledge, the current study is the first research that used this species as a source of collagen. Both ASC-S and ASC-B from mangar skin and bone contained glycine as the major amino acid and high amounts of proline, hydroxyproline, alanine, and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were 9.38 and 3.71%, respectively. Furthermore, fourier transform infrared spectroscopy proved that both collagens were integrated and native. Additionally, the results of XRD demonstrated that both of the collagens reserved their helical structures. The screened collagens had prominent absorptions at 230 nm by UV-Vis spectra. Additionally, the SEM studies have shown that both ACS-S and ASC-B have porous and fibrous nature. According to the UV–Vis and FTIR results, extracted collagens were characterized as type I collagen based on their amino acid composition. According to the obtained results, the collagen isolated from mangar can potentially be an alternative source of vertebrate collagens for use in the diet and other industries such as medical and pharmaceutical industries.