Araştırma Makalesi
BibTex RIS Kaynak Göster

ELAJİK ASİDİN İNSAN KOLON KANSERİ HÜCRELERİNDE İNCELENMESİ

Yıl 2023, Cilt: 7 Sayı: 2, 72 - 81, 29.12.2023
https://doi.org/10.47137/usufedbid.1323084

Öz

Kanser çağımızın en önemli ve en ölümcül hastalıklardan biri olarak görülmektedir. Kanser türlerinden biri olan ve kansere bağlı ölüm vakalarında ikinci sırada yer alan kolon kanseri erkekler bireylerde en yaygın ikinci, kadınlarda ise en yaygın dördüncü kanser tipidir. Elajik asit çilek, ahududu, fındık gibi meyve ve sebzelerden elde edilebilen ve insan sağlığı açısından oldukça önemli bir bitkisel fitokimyasaldır. Elajik asidin insan kolon kanseri üzerindeki etkilerinin anlaşılması için, farklı dozlarda elajik asit uygulanmıştır. Yapılan proliferasyon çalışmasında CCL-233 hücrelerine 5 µM, 10 µM, 25 µM, 50 µM ve 100 µM konsantrasyonlarında elajik asit uygulanmıştır. Uygulanan elajik asit konsantrasyonun artması ile beraber hücrelerin ölümü gözlemlenmiştir. Yarı öldürücü doz (LD50) 40 µM olarak tayin edilmiştir. PARP ve VEGF çalışmaları için insan kolon hücrelerine farklı dozlarda elajik asit uygulanmış ve sonuç olarak iki değerde de azalma görülmüştür.

Kaynakça

  • Parkin D, Bray F, Ferlay J, & Pisani P. CA Cancer Journal for Clinicals, Global Cancer Statistics, 2005; 74-108.
  • Lai S, Zhang K, Uhler R, Harrison J, Clutter G, Williams M. Geographic variation in the incidence of colorectal cancer in The United States, 1998-2001. Cancer, 2005;117(5):1172-1180.
  • Haggar F, Boushey R. Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clinics in Colon and Rectal Surgery, 2009;22(4):191-197.
  • Block G, Patterson B, Subar A. Fruit, vegetables, and cancer prevention: A review of epidemiological evidence. Nutrition and Cancer, 1992;18(1):1-29.
  • Serdula M, Byers T, Simoes E, Mendlein J, Coates R. The association between fruit and vegetable intake and chronic disease risk factors. Epidemiology, 1996;7(2):161-165.
  • Akamura Y, Okada M, Tsuji S, Tonogai Y. Determination of phenolic acids in fruit juices by isocratic column liquid chromatography. Journal of Chromatography A, 2000;891(1):183–188.
  • Marwan A, Nagel C. Characterization of cranberry benzoates and their antimicrobial properties. Journal of Food Science, 1986;51(4):1069–1070.
  • Chen H, Zuo Y, Deng Y. Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. Journal of Chromatography A, 2001; 913(1-2):387–395.
  • Zhang Z, Hamilton S, Stewart C, Strother A, Teel R. Inhibition of liver microsomal cytochrome P450 activity and metabolism of the tobacco-specific nitrosamine NNK by capsaicin and ellagic acid. Anticancer Research, 1993;13(6A):2341–2346.
  • Labianca R, Beretta G, Kildani B, Milesi L, Merlin F, Mosconi S, Wils J. Colon cancer. Critical Reviews in Oncology/Hematology, 2010;74(2):106-133.
  • Fang Y, Zhou H, Xia JF, Lin JJ, Li RZ, Yang DQ, Li XY. Ellagic acid regulates Wnt/β-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells. Bangladesh Journal of Pharmacology, 2015;10(1):47-56.
  • Zhao M, Tang SN, Marsh J, Shankar S, Srivastava R. Ellagic acid inhibits human pancreatic cancer growth in Balb c nude mice. Cancer Letters, 2013;337(2):210-217.
  • Pitchakarn P, Chewonarin T, Ogawa K, Suzuki S, Asamoto M, Takahashi S, Limtrakul P. Ellagic Acid Inhibits Migration and Invasion by Prostate Cancer Cell Lines. Asian Pacific Journal of Cancer Prevention, 2013;14(5):2859-2863.
  • de Murcia G, Menissier-de Murcia J. Poly (ADP-ribose) polymerase: a molecular nick-sensor. Trends in Biochemical Sciences, 1994;19(4):172-176.
  • Zhao Z, Xia G, Li N, Su R, Chen X, Zhong L. Autophagy Inhibition Promotes Bevacizumab-induced Apoptosis and Proliferation Inhibition in Colorectal Cancer Cells. Journal of Cancer, 2018;9(18):3407-3416.
  • Jaman S, Sayeed A. Ellagic acid, sulforaphane, and ursolic acid in the prevention and therapy of breast cancer: current evidence and future perspectives. Breast Cancer, 2018;25(5):517-528.
  • Ceci C, Tentori L, Atzori MG, Lacal PM, Bonanno E, Scimeca M, Cicconi R, Mattei M, de Martino MG, Vespasiani G, Miano R, Graziani G. Ellagic Acid Inhibits Bladder Cancer Invasiveness and In Vivo Tumor Growth. Nutrients, 2016;8(11):744.
  • Cheshomi H, Bahrami AR, Rafatpanah H, Matin MM. The effects of ellagic acid and other pomegranate (Punica granatum L.) derivatives on human gastric cancer AGS cells. Human and Experimental Toxicology, 2022;41:1-16.
  • Xiong Ni, Fu-Sheng Shang, Ting-Feng Wang, De-Jun Wu, Da-Gui Chen, Biao Zhuang. Ellagic acid induces apoptosis and autophagy in colon cancer through the AMPK/mTOR pathway. Tissue and Cell, 2023; 81:102032.
  • Vanella L, Di Giacomo C, Acquaviva R, Barbagallo I, Li Volti G, Cardile V, Abraham NG, Sorrenti V. Effects of ellagic acid on angiogenic factors in prostate cancer cells. Cancers, 2013;5(2):726-738.
  • Wang N, Wang ZY, Mo SL, Loo TY, Wang DM, Luo HB, Yang DP, Chen YL, Shen JG, Chen JP. Ellagic acid, a phenolic compound, exerts anti-angiogenesis effects via VEGFR-2 signaling pathway in breast cancer. Breast Cancer Research and Treatment, 2012;134(3):943-955.

INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS

Yıl 2023, Cilt: 7 Sayı: 2, 72 - 81, 29.12.2023
https://doi.org/10.47137/usufedbid.1323084

Öz

Cancer is seen as one of the most important and deadly diseases of our time. Colon cancer, which is one of the cancer types and ranks second in cancer-related deaths, is the second most common cancer type in men and the fourth most common cancer type in women. Ellagic acid is a plant phytochemical that can be obtained from fruits and vegetables such as strawberries, raspberries, and hazelnuts and is very important for human health. In order to understand the effects of ellagic acid on human colon cancer, different doses of ellagic acid were applied. In the proliferation study, ellagic acid was applied to CCL-233 cells at concentrations of 5 µM, 10 µM, 25 µM, 50 µM and 100 µM. Cell death was observed with an increase in applied ellagic acid concentration. The semi-lethal dose (LD50) was determined to be 40 µM. For PARP and VEGF studies, different doses of ellagic acid were applied to human colon cancer cells, which consequently showed a decrease in both values.

Kaynakça

  • Parkin D, Bray F, Ferlay J, & Pisani P. CA Cancer Journal for Clinicals, Global Cancer Statistics, 2005; 74-108.
  • Lai S, Zhang K, Uhler R, Harrison J, Clutter G, Williams M. Geographic variation in the incidence of colorectal cancer in The United States, 1998-2001. Cancer, 2005;117(5):1172-1180.
  • Haggar F, Boushey R. Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clinics in Colon and Rectal Surgery, 2009;22(4):191-197.
  • Block G, Patterson B, Subar A. Fruit, vegetables, and cancer prevention: A review of epidemiological evidence. Nutrition and Cancer, 1992;18(1):1-29.
  • Serdula M, Byers T, Simoes E, Mendlein J, Coates R. The association between fruit and vegetable intake and chronic disease risk factors. Epidemiology, 1996;7(2):161-165.
  • Akamura Y, Okada M, Tsuji S, Tonogai Y. Determination of phenolic acids in fruit juices by isocratic column liquid chromatography. Journal of Chromatography A, 2000;891(1):183–188.
  • Marwan A, Nagel C. Characterization of cranberry benzoates and their antimicrobial properties. Journal of Food Science, 1986;51(4):1069–1070.
  • Chen H, Zuo Y, Deng Y. Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. Journal of Chromatography A, 2001; 913(1-2):387–395.
  • Zhang Z, Hamilton S, Stewart C, Strother A, Teel R. Inhibition of liver microsomal cytochrome P450 activity and metabolism of the tobacco-specific nitrosamine NNK by capsaicin and ellagic acid. Anticancer Research, 1993;13(6A):2341–2346.
  • Labianca R, Beretta G, Kildani B, Milesi L, Merlin F, Mosconi S, Wils J. Colon cancer. Critical Reviews in Oncology/Hematology, 2010;74(2):106-133.
  • Fang Y, Zhou H, Xia JF, Lin JJ, Li RZ, Yang DQ, Li XY. Ellagic acid regulates Wnt/β-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells. Bangladesh Journal of Pharmacology, 2015;10(1):47-56.
  • Zhao M, Tang SN, Marsh J, Shankar S, Srivastava R. Ellagic acid inhibits human pancreatic cancer growth in Balb c nude mice. Cancer Letters, 2013;337(2):210-217.
  • Pitchakarn P, Chewonarin T, Ogawa K, Suzuki S, Asamoto M, Takahashi S, Limtrakul P. Ellagic Acid Inhibits Migration and Invasion by Prostate Cancer Cell Lines. Asian Pacific Journal of Cancer Prevention, 2013;14(5):2859-2863.
  • de Murcia G, Menissier-de Murcia J. Poly (ADP-ribose) polymerase: a molecular nick-sensor. Trends in Biochemical Sciences, 1994;19(4):172-176.
  • Zhao Z, Xia G, Li N, Su R, Chen X, Zhong L. Autophagy Inhibition Promotes Bevacizumab-induced Apoptosis and Proliferation Inhibition in Colorectal Cancer Cells. Journal of Cancer, 2018;9(18):3407-3416.
  • Jaman S, Sayeed A. Ellagic acid, sulforaphane, and ursolic acid in the prevention and therapy of breast cancer: current evidence and future perspectives. Breast Cancer, 2018;25(5):517-528.
  • Ceci C, Tentori L, Atzori MG, Lacal PM, Bonanno E, Scimeca M, Cicconi R, Mattei M, de Martino MG, Vespasiani G, Miano R, Graziani G. Ellagic Acid Inhibits Bladder Cancer Invasiveness and In Vivo Tumor Growth. Nutrients, 2016;8(11):744.
  • Cheshomi H, Bahrami AR, Rafatpanah H, Matin MM. The effects of ellagic acid and other pomegranate (Punica granatum L.) derivatives on human gastric cancer AGS cells. Human and Experimental Toxicology, 2022;41:1-16.
  • Xiong Ni, Fu-Sheng Shang, Ting-Feng Wang, De-Jun Wu, Da-Gui Chen, Biao Zhuang. Ellagic acid induces apoptosis and autophagy in colon cancer through the AMPK/mTOR pathway. Tissue and Cell, 2023; 81:102032.
  • Vanella L, Di Giacomo C, Acquaviva R, Barbagallo I, Li Volti G, Cardile V, Abraham NG, Sorrenti V. Effects of ellagic acid on angiogenic factors in prostate cancer cells. Cancers, 2013;5(2):726-738.
  • Wang N, Wang ZY, Mo SL, Loo TY, Wang DM, Luo HB, Yang DP, Chen YL, Shen JG, Chen JP. Ellagic acid, a phenolic compound, exerts anti-angiogenesis effects via VEGFR-2 signaling pathway in breast cancer. Breast Cancer Research and Treatment, 2012;134(3):943-955.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyokimya ve Hücre Biyolojisi (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Muhammed Ali Çınar 0000-0001-5401-5900

Doç. Dr. Funda Karabağ 0000-0002-1565-3210

Yayımlanma Tarihi 29 Aralık 2023
Gönderilme Tarihi 5 Temmuz 2023
Kabul Tarihi 25 Temmuz 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 7 Sayı: 2

Kaynak Göster

APA Çınar, M. A., & Karabağ, D. D. F. (2023). INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS. Uşak Üniversitesi Fen Ve Doğa Bilimleri Dergisi, 7(2), 72-81. https://doi.org/10.47137/usufedbid.1323084
AMA Çınar MA, Karabağ DDF. INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi. Aralık 2023;7(2):72-81. doi:10.47137/usufedbid.1323084
Chicago Çınar, Muhammed Ali, ve Doç. Dr. Funda Karabağ. “INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS”. Uşak Üniversitesi Fen Ve Doğa Bilimleri Dergisi 7, sy. 2 (Aralık 2023): 72-81. https://doi.org/10.47137/usufedbid.1323084.
EndNote Çınar MA, Karabağ DDF (01 Aralık 2023) INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi 7 2 72–81.
IEEE M. A. Çınar ve D. D. F. Karabağ, “INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS”, Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi, c. 7, sy. 2, ss. 72–81, 2023, doi: 10.47137/usufedbid.1323084.
ISNAD Çınar, Muhammed Ali - Karabağ, Doç. Dr. Funda. “INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS”. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi 7/2 (Aralık 2023), 72-81. https://doi.org/10.47137/usufedbid.1323084.
JAMA Çınar MA, Karabağ DDF. INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi. 2023;7:72–81.
MLA Çınar, Muhammed Ali ve Doç. Dr. Funda Karabağ. “INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS”. Uşak Üniversitesi Fen Ve Doğa Bilimleri Dergisi, c. 7, sy. 2, 2023, ss. 72-81, doi:10.47137/usufedbid.1323084.
Vancouver Çınar MA, Karabağ DDF. INVESTIGATION OF ELLAGIC ACID IN HUMAN COLON CANCER CELLS. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi. 2023;7(2):72-81.