Araştırma Makalesi
Yıl 2017,
Cilt: 1 Sayı: 1, 20 - 23, 01.12.2017
Öz
Kaynakça
- Akinwande KL, Badejo MA, Ogbogu SS. Incidence of the Korean haplotype of Varroa destructor in southwest Nigeria. J Apic Res 2012; 51:369-70.
- Amdam GV, Hartfelder K, Norberg K, Hagen A, Omholt SW. Altered physiology in worker honey bees (Hymenoptera: Apidae) infested with the mite Varroa destructor (Acari: Varroidae): a factor in colony loss during overwintering? J Econ Entomol 2004; 97:741-747.
- Anderson DL, Fuchs S. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. J Apic Res 1998; 37: 69-78.
- Anderson DL, Trueman JWH. Varroa jacobsoni (Acari: Varroidae) is more than one species. Exp Appl Acarol 2000; 24:165-189.
- Duay P, De Jong D, Engels W. Weight loss in drone pupae (Apis mellifera) multiply infested by Varroa destructor mites. Apidologie 2003; 34:61-65.
- Fazier M, Muli E, Conklin T, et al. A scientific note on Varroa destructor found in East Africa; threat or opportunity? Apidologie 2010; 41:463-465
- Gajic B, Radulovic Z, Stevanovic J, et al. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Exp Appl Acarol 2013; 61:97-105.
- Garedew A, Schmolz E, Lamprecht I.The energy and nutritional demand of the parasitic life of the mite Varroa destructor. Apidologie 2004; 35:419-430.
- Guerra JCV Jr, Iss MRC, Carneiro FE, Strapazzon R, Moretto G. Genetics and molecular research RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas. Genet Mol Res 2010; 9:303-308.
- Marcangeli JA, Eguaras MJ, Fernandez NA. Reproduction of Varroa jacobsoni (Acari: Mesostigmata: Varroidae) in temperate climates of Argentina. Apidologie 1992; 23:57-60.
- Navajas M, Anderson DL, De Guzman LI, et al. New Asian types of Varroa destructor: a potential new threat for world apiculture. Apidologie 2010; 41:181-193.
- Rasolofoarivao H, Clemencet J, Ravaomanarivo LH, Razafindrazaka D, Reynaud B, Delatte H. Spread and strain determination of Varroa destructor (Acari: Varroidae) in Madagascar since its first report in 2010. Exp Appl Acarol 2013; 60:521-530
- Rosenkranz P, Aumeier P, Ziegelmann B. Biology and control of Varroa destructor. J Invertebr Pathol 2010; 103:96-119.
- Solignac M, Cornuet JM, Vautrin D, et al. The invasive Korea and Japan types of Varroa destructor, ectoparasitic mites of the Western honeybee (Apis mellifera), are two partly isolated clones. Proc R Soc Lond B Biol Sci 2005; 272:411-419.
- Strapazzon R, Carneiro FE, Guerra Jr JCV, Moretto G. Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the state of Santa Catarina, Brazil. Genet Mol Res 2009; 8:990-997.
- Tentcheva D, Gauthier L, Bagny L, et al. Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera and Varroa destructor. Apidologie 2006; 37:41-50.
- Warrit N, Hagen TAR, Smith DR, Cakmak IA. Survey of Varroa derstructor strains on Apis mellifera in Turkey. J Apic Res 2004; 3:190-191.
Analysis of COI gene region of Varroa destructor in honey bees (Apis mellifera) in province of Siirt
Öz
ABSTRACT
Objectives: Varroa destructor is the most damaging ectoparasite to the beekeeping economy. The mite has
different haplotypes. It is aimed to determine which haplotype is present by examining the cytochrome c oxidase
subunit 1 (COI) gene region of V. destructor found in honey bees in Siirt region.
Materials and Methods: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism
(RFLP) methods were applied in the analysis of the COI gene region of V. destructor in Siirt region. To do this,
V. destructor samples were collected from 387 enterprises in the Siirt region. DNA extraction followed the PZR.
Subsequently, 1.5% agarose gel images were obtained by electrophoresis. The PCR products were then subjected
to XhoI and SacI restriction enzymes and 2% agarose gel images were obtained. 38 of the samples (10%) were
sent to a private enterprise for sequencing. The obtained sequences were blasted and compared with the
corresponding reference sequences in GenBank.
Results: According to the results of PZR and RFLP obtained from the 387 V. destructor samples in the study
towards the COI gen region, all of the samples were found to be Korean haplotypes and Japanese haplotypes
were not found in any of 387 samples. At the same time, it was also confirmed that the 38 sequenced samples
were Korean haplotypes.
Discussion: The results obtained from this study are significant in terms of forming a groundwork for future
studies.
Anahtar Kelimeler
Kaynakça
- Akinwande KL, Badejo MA, Ogbogu SS. Incidence of the Korean haplotype of Varroa destructor in southwest Nigeria. J Apic Res 2012; 51:369-70.
- Amdam GV, Hartfelder K, Norberg K, Hagen A, Omholt SW. Altered physiology in worker honey bees (Hymenoptera: Apidae) infested with the mite Varroa destructor (Acari: Varroidae): a factor in colony loss during overwintering? J Econ Entomol 2004; 97:741-747.
- Anderson DL, Fuchs S. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. J Apic Res 1998; 37: 69-78.
- Anderson DL, Trueman JWH. Varroa jacobsoni (Acari: Varroidae) is more than one species. Exp Appl Acarol 2000; 24:165-189.
- Duay P, De Jong D, Engels W. Weight loss in drone pupae (Apis mellifera) multiply infested by Varroa destructor mites. Apidologie 2003; 34:61-65.
- Fazier M, Muli E, Conklin T, et al. A scientific note on Varroa destructor found in East Africa; threat or opportunity? Apidologie 2010; 41:463-465
- Gajic B, Radulovic Z, Stevanovic J, et al. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Exp Appl Acarol 2013; 61:97-105.
- Garedew A, Schmolz E, Lamprecht I.The energy and nutritional demand of the parasitic life of the mite Varroa destructor. Apidologie 2004; 35:419-430.
- Guerra JCV Jr, Iss MRC, Carneiro FE, Strapazzon R, Moretto G. Genetics and molecular research RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas. Genet Mol Res 2010; 9:303-308.
- Marcangeli JA, Eguaras MJ, Fernandez NA. Reproduction of Varroa jacobsoni (Acari: Mesostigmata: Varroidae) in temperate climates of Argentina. Apidologie 1992; 23:57-60.
- Navajas M, Anderson DL, De Guzman LI, et al. New Asian types of Varroa destructor: a potential new threat for world apiculture. Apidologie 2010; 41:181-193.
- Rasolofoarivao H, Clemencet J, Ravaomanarivo LH, Razafindrazaka D, Reynaud B, Delatte H. Spread and strain determination of Varroa destructor (Acari: Varroidae) in Madagascar since its first report in 2010. Exp Appl Acarol 2013; 60:521-530
- Rosenkranz P, Aumeier P, Ziegelmann B. Biology and control of Varroa destructor. J Invertebr Pathol 2010; 103:96-119.
- Solignac M, Cornuet JM, Vautrin D, et al. The invasive Korea and Japan types of Varroa destructor, ectoparasitic mites of the Western honeybee (Apis mellifera), are two partly isolated clones. Proc R Soc Lond B Biol Sci 2005; 272:411-419.
- Strapazzon R, Carneiro FE, Guerra Jr JCV, Moretto G. Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the state of Santa Catarina, Brazil. Genet Mol Res 2009; 8:990-997.
- Tentcheva D, Gauthier L, Bagny L, et al. Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera and Varroa destructor. Apidologie 2006; 37:41-50.
- Warrit N, Hagen TAR, Smith DR, Cakmak IA. Survey of Varroa derstructor strains on Apis mellifera in Turkey. J Apic Res 2004; 3:190-191.
Toplam 17 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | 2017 Volume 1 Number 1 |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 2017 |
| Gönderilme Tarihi | 30 Kasım 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 1 Sayı: 1 |
Kaynak Göster
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