Öz
In the present study, the antibacterial activity of the crude extracts obtained from flowers, leaves and stems of HypericumcalycinumL.against Streptococcus mitis CNCTC 4/77, Streptococcus salivarius CNCTC 64/59, Streptococcus mutans CNCTC 8/77 andStaphylococcus epidermidis ATCC 12228 was determined in vitro. Dried powder of flower, leave and stem parts of this plant were kept in acetone and methanol for 3 days in dark place. Disc diffusion method was used to determine the antibacterial activity of Hypericumcalycinum L. Methanol-impregnated discs and acetone-impregnated discs were used as negative control. Also commercial antibiotic discs (Clindamycin (10 μg), Tetracycline (10 μg) and Amoxicillin-Clavulanic acid (30 μg)) were utilized as positive control.According to the results of the present study, the crude extracts ofHypericumcalycinum L. prepared in acetone and methanol have been found to have antibacterial activity against the test microorganisms. Extracts of Hypericumcalycinum L.flowershowed the largest inhibition zone (13 mm in diameter) againstS. mutans.The antibacterial activities of methanol and acetone extracts ofHypericumcalycinum L.(at the doses of 3200 μg/10μl, 1600 μg/10μl, 800 μg/10μl and 400 μg/10μl)against Streptococcus salivarius, Streptococcus mutans, Staphylococcus epidermidis andStreptococcus mitis have been reported in this study for the first time.
Anahtar Kelimeler
Antibacterial activity Disc diffusion method Hypericumcalycinum L.
Kaynakça
- Crockett S.L., Robson N.K.B. 2011. Taxonomy and chemotaxonomy of the genus Hypericum.Med Aromat Plant SciBiotechnol. 5(1):1–13.
- Çırak C., Radusiene J., Aksoy H.M., Mackinaite R., Stanius Z., Camas N., Odabas M.S. 2014. Differential phenolic accumulation in two Hypericumspecies in response to inoculation with Diplocerashypericinumand Pseudomonas putida.Plant Protect. Sci. 50(3):119–128.
- Darwish R.M., Aburjai T.A. 2010. Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli.BMC Complementary and Alternative Medicine.10:9.
- Dülger B., Gönüz A., Hacıoğlu N. 2007. HypericumcalycinumL.’unantimikrobiyalaktivitesi, Publications of ÇanakkaleOnsekiz Mart University, ISBN: 978–975–8100–65–1, 105-108, Bayramiç Symposium, Çanakkale, Turkey.
- Fenner R., Sortino M., Rates S.M.K., Dall’Agnol R., Ferraz A., Bernardi A.P., Albring D., Nör C., Poser G.V., Schapoval E., Zacchino S. 2005. Antifungal activity of some Brazilian Hypericum species.Phytomedicine. 12:236–240.
- Ferretti G., Maggi F., Tirillini B. 2005. Essential oil composition of HypericumricheriVill.from Italy. FlavourFragr. J.20:295–298.
- Galati E.M., Contartese G., Miceli N., Taviano M.F., Sdrafkakis V., Couladis M., Tzakou O., Lanuzza F. 2008. Antiinflammatory and antioxidant activity of HypericumrumeliacumBoiss.subsp. apollinis(Boiss. &Heldr.) Robson &Strid methanol extract. Phytother. Res. 22:766-771.
- Gibbons S., Ohlendorf B., Johnsen I. 2002. The genus Hypericum a valuable resource of anti-Staphylococcal leads.Fitoterapia. 73:300-304. Gottshall R.Y., Lucas E. H., Lickfeldt A., Roberts J. M. 1949. The occurrence of antibacterial substances active against Mycobacterium tuberculosis in seed plants, Second National Symposium on Recent Advances in Antibiotics Research held in Washington, D. C., April 11-12, under the auspices of the Antibiotics Study Section, National Institutes of Health, Public Health Service, Federal Security Agency. Maggi F., Cecchini C., Cresci A., Coman M.M., Tirillini B., Sagratini G., Papa F., Vittori S. 2010. Chemical composition and antimicrobial activity of the essential oils from several Hypericum taxa (Guttiferae) growing in central Italy (AppenninoUmbro-Marchigiano). Chemistry & Biodiversity. 7:447-466.
- Nogueira T., Medeiros M.A., Marcelo-Curto M.J., García-Pérez B.E., Luna-Herrera J., Costa M.C. 2013.Profile of antimicrobial potential of fifteen Hypericumspecies from Portugal.Industrial Crops and Products. 47:126–131.
- Özkan A.M.G., Demirci B., Başer K.H.C. 2009. Essential oil composition of HypericumthymopsisBoiss.Journal of Essential Oil Research. 21:149-153.
- Özkan E.E., Mat A. 2013. An overview on Hypericumspecies of Turkey.Journal of Pharmacognosy and Phytotherapy. 5(3):38-46.
- Pavlović M., TzakouO., Petrakis P.V., Couladis M. 2006. The essential oil of HypericumperforatumL., Hypericumtetrapterumfries and HypericumolympicumL. growing in Greece. FlavourFragr. J.21:84–87.
- Petrelli D., Buonanno F., Vitali L.A., Ortenzi C. 2012. Antimicrobial activity of the protozoan toxin climacostol and its derivatives.Biologia. 67(3):525-529.
- Sakar M.K., Tamer A.Ü. 1990. Antimicrobial activity of different extracts from some Hypericumspecies. Fitoterapia.vol.LXI(5):464-466.
- Sarkisian S.A., Janssen M.J., Matta H., Henry G.E., LaPlante K.L., Rowley D.C. 2012. Inhibition of bacterial growth and biofilm production by constituents from Hypericum spp. Phytother. Res. 26:1012–1016.
- Schiavone B.I.P., Rosato A., Marilena M., Gibbons S., Bombardelli E., Verotta L., Franchini C., and Corbo F. 2013. Biological evaluation of hyperforin and its hydrogenated analogue on bacterial growth and biofilm production.Journal of natural products. 76:1819-1823.
- Şerbetçi T., Özsoy N., Demirci B., Can A., Kültür Ş., Başer K.H.C. 2012. Chemical composition of the essential oil and antioxidant activity of methanolic extracts from fruits and flowers of HypericumlydiumBoiss.Industrial Crops and Products.36:599–606.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Ziraat Mühendisliği |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 31 Aralık 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 10 Sayı: 2 |