Araştırma Makalesi
Alzheimer Hastalığının Tedavisinde Kullanılan Memantin ve Donepezil Etken Maddelerinin Spektrofotometrik Verilerin Değerlendirilmesi ile Kemometrik Tayini
Öz
Alzheimer tedavisinde kullanılan memantin ve donepezil içeren ilaç numunesini analiz etmek için yapılmış bu çalışmada, UV Görünür Bölge Spektroskopisi, kemometri tayini yöntemi ile birlikte kullanılmıştır. Memantin ve donepezil etken maddelerinin ultraviyole spektrumları ölçülerek kaydedilmiş, çalışılabilecek saflık değerinde olduğu görülmüş ve analiz çalışması yapılmıştır. Uyguladığımız yöntemde, memantin ve donepezil için yüzde (%) geri kazanımı ortalaması sentetik karışım için ve ilaç numunesi için hesaplanmıştır. Hesaplanan değerler, oldukça yüksektir. Kullanılan PLS ve PCR kalibrasyon tekniklerinden ulaşılan sonuçlara ANOVA testi yapılarak yöntemlerin kontrolü sağlanmıştır. Geliştirilmiş yöntemler, tekrar edilebilir, duyarlılığı hassas ve doğru sonuç veren yöntemlerden biri olup memantin ve donepezil içeren ilaç numunelerinin analizinde önerilebilir.
Destekleyen Kurum
Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Proje Numarası
FYL-2019-7352
Teşekkür
Bu çalışma, Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından “FYL-2019-7352” kodlu proje ile desteklenmiştir.
Kaynakça
- Aktaş, A.H., Sarıdağ A.M. (2017). Liquid chromatografic-chemometric tecnhniques for the simultaneous HPLC determination of lansoprazole, amoksisilin and clarithromisin in commercial preparation. Journal of Chromatografic Science, 1-7.
- Armbruster, D.A., Pty, T. (2008). Limit of blank, limit of detection and limit of quantitation , Clin Biochem Rev, 29, 49-52.
- Bajpai, V., Kumar, S., Singh, A., Singh, J., Negi, M.P.S., Bag, S.K., Kumar, N., Konwar, R., Kumar, B. (2017). Chemometric based identification and validation of specific chemical markers for goegraphical, seasonal and gender variations in tinospora cordifolia stem using HPLC-ESI-QTOF-MS Analysis, Photochemical Analysis, 28 (4), 277-288.
- Bilgili, A.V., Çullu, M.A., Aydemir, S. (2014). Tuzdan etkilenmiş toprakların yakın kızılötesi yansıma spektroradyometre ve elektromanyetik indüksiyon tekniği yardımıyla karakterize edilebilme potansiyelinin araştırılması, Harran Tarım ve Gıda Bilimleri Dergisi, 18 (1), 32-45.
- Dinç, E. (2009). Kemometrik İşlem ve Yöntemlerin Analitik Kimyadaki Tipik Uygulamaları, Uygulamalı Kemometri Yaz Okulu Notları, 13-17.
- Dinç, E., Baleanu, D. (2002). Spectrophotometric quantitative determination of cilazapril and hydrochlorothiazide in tablets by chemometric methods, Journal of Pharmaceutical and Biomedical Analysis, 30, 715-723.
- Dinç, E., Özdemir, A., Baleanu, D. (2005). Comparative study of the continuous wavelent transform, derivative and partial least squares methods applied to the overlapping spectra for the simultaneous quantitative resolution of ascorbic acid and asetilsalicylic acid in effervescent tablets, Journal of Pharmaceutical and Biomedical Analysis, 37, 569-575.
- Miao, J., Forget, B., Smith, K. (2018). Predicting Correlation Coefficients for Monte Carlo Eigenvalue Simulations With Multitype Branching Process, Annals of Nuclear Energy, 112, 307-321.
- Porfire A., Muntean, D., Achim, M., Vlase, L., Tomuta, I. (2015). Simultaneous Quantification of Simvastatin and Excipients in Liposomes Using Near Spectroscopy and Chemometry, Journal of Pharmaceutical and Biomedical Analysis, 107, 40-49.
- Ravikumar, R., Ganesh, M., Jang, H.T. (2016). Validated stability indicating simultaneous estimation of memantine and donepezil in pharmaceutical formulation by RP-HPLC-DAD, DerPharmacia Lettre, 8 (15), 79-87.
- Saganowska, P., Wesolowski, M. (2017). Principal Component and Cluster Analyses as Supporting Tools for Co-Crystals Detection, J.Therm.Anal.Calorim., 130, 45-55.
- Sharma, D., Singh, R., Garg, R. (2017). Development and validation of stability indicating UV spectro-photometric method for the estimation of benzydamine hydrochloride in bulk and in pharmaceutical dosage form: a novel analytical technique for conducting in-vitro quality control tests, International Journal Of Pharmaceutical Sciences And Research, 9(2), 678-686.
- Shrivastava, A., Gupta, V.B. (2011). Methods for the determination of limit of detection and limit of quantitation of the analytical methods, Chronicles of Young Scientist, 2 (1), 21-25.
- Tarhan, I., Kara, A.A.I.H. (2017). Quantitative Determination of Free Fatty Acids in Extra Virgin Olive Oils by Multivariate Methods and Fourier Transform Infrared Spectroscopy Considering Different Absorption Modes, International Journal of Food Properties, 1-8.
- Uyanık, A. (2012). Analitik Kimyacılar İçin İstatistik ve Kemometri, Pegem Akademi Yayıncılık, 6. Baskı, 254-259.
- Yongnian, N., Gong X. (1997). Simultaneous Spectrophotometric Determination of Mixtures of Food Colorants. Analytica Chimica Acta, 354, 163-171.
- Yüksel, F. V. (2006, November 15). Demans Nedir?. http://antalyapsikoloji.com
Chemometric Determination of Memantine and Donepezil Active Ingredients Used in the Treatment of Alzheimer's Disease by Evaluation of Spectrophotometric Data
Öz
In this study, which was conducted to analyze the drug sample used in Alzheimer's treatment containing memantine and donepezil, UV Visible Spectroscopy was used together with the chemometry determination method. The ultraviolet spectra of the active ingredients of memantine and donepezil were measured and recorded, they were found to be of workable purity and analysis was carried out. In the method we applied, the mean percent (%) recovery for memantine and donepezil was calculated for the synthetic mixture and for the drug sample. The calculated values are quite high. The results obtained from the PLS and PCR calibration techniques used were controlled by the ANOVA test. The developed methods are one of the reproducible, sensitive and accurate methods and can be recommended for the analysis of drug samples containing memantine and donepezil.
Anahtar Kelimeler
Proje Numarası
FYL-2019-7352
Kaynakça
- Aktaş, A.H., Sarıdağ A.M. (2017). Liquid chromatografic-chemometric tecnhniques for the simultaneous HPLC determination of lansoprazole, amoksisilin and clarithromisin in commercial preparation. Journal of Chromatografic Science, 1-7.
- Armbruster, D.A., Pty, T. (2008). Limit of blank, limit of detection and limit of quantitation , Clin Biochem Rev, 29, 49-52.
- Bajpai, V., Kumar, S., Singh, A., Singh, J., Negi, M.P.S., Bag, S.K., Kumar, N., Konwar, R., Kumar, B. (2017). Chemometric based identification and validation of specific chemical markers for goegraphical, seasonal and gender variations in tinospora cordifolia stem using HPLC-ESI-QTOF-MS Analysis, Photochemical Analysis, 28 (4), 277-288.
- Bilgili, A.V., Çullu, M.A., Aydemir, S. (2014). Tuzdan etkilenmiş toprakların yakın kızılötesi yansıma spektroradyometre ve elektromanyetik indüksiyon tekniği yardımıyla karakterize edilebilme potansiyelinin araştırılması, Harran Tarım ve Gıda Bilimleri Dergisi, 18 (1), 32-45.
- Dinç, E. (2009). Kemometrik İşlem ve Yöntemlerin Analitik Kimyadaki Tipik Uygulamaları, Uygulamalı Kemometri Yaz Okulu Notları, 13-17.
- Dinç, E., Baleanu, D. (2002). Spectrophotometric quantitative determination of cilazapril and hydrochlorothiazide in tablets by chemometric methods, Journal of Pharmaceutical and Biomedical Analysis, 30, 715-723.
- Dinç, E., Özdemir, A., Baleanu, D. (2005). Comparative study of the continuous wavelent transform, derivative and partial least squares methods applied to the overlapping spectra for the simultaneous quantitative resolution of ascorbic acid and asetilsalicylic acid in effervescent tablets, Journal of Pharmaceutical and Biomedical Analysis, 37, 569-575.
- Miao, J., Forget, B., Smith, K. (2018). Predicting Correlation Coefficients for Monte Carlo Eigenvalue Simulations With Multitype Branching Process, Annals of Nuclear Energy, 112, 307-321.
- Porfire A., Muntean, D., Achim, M., Vlase, L., Tomuta, I. (2015). Simultaneous Quantification of Simvastatin and Excipients in Liposomes Using Near Spectroscopy and Chemometry, Journal of Pharmaceutical and Biomedical Analysis, 107, 40-49.
- Ravikumar, R., Ganesh, M., Jang, H.T. (2016). Validated stability indicating simultaneous estimation of memantine and donepezil in pharmaceutical formulation by RP-HPLC-DAD, DerPharmacia Lettre, 8 (15), 79-87.
- Saganowska, P., Wesolowski, M. (2017). Principal Component and Cluster Analyses as Supporting Tools for Co-Crystals Detection, J.Therm.Anal.Calorim., 130, 45-55.
- Sharma, D., Singh, R., Garg, R. (2017). Development and validation of stability indicating UV spectro-photometric method for the estimation of benzydamine hydrochloride in bulk and in pharmaceutical dosage form: a novel analytical technique for conducting in-vitro quality control tests, International Journal Of Pharmaceutical Sciences And Research, 9(2), 678-686.
- Shrivastava, A., Gupta, V.B. (2011). Methods for the determination of limit of detection and limit of quantitation of the analytical methods, Chronicles of Young Scientist, 2 (1), 21-25.
- Tarhan, I., Kara, A.A.I.H. (2017). Quantitative Determination of Free Fatty Acids in Extra Virgin Olive Oils by Multivariate Methods and Fourier Transform Infrared Spectroscopy Considering Different Absorption Modes, International Journal of Food Properties, 1-8.
- Uyanık, A. (2012). Analitik Kimyacılar İçin İstatistik ve Kemometri, Pegem Akademi Yayıncılık, 6. Baskı, 254-259.
- Yongnian, N., Gong X. (1997). Simultaneous Spectrophotometric Determination of Mixtures of Food Colorants. Analytica Chimica Acta, 354, 163-171.
- Yüksel, F. V. (2006, November 15). Demans Nedir?. http://antalyapsikoloji.com
Toplam 17 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Mühendislik |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Proje Numarası | FYL-2019-7352 |
| Yayımlanma Tarihi | 31 Aralık 2021 |
| Gönderilme Tarihi | 10 Eylül 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 1 Sayı: 2 |
