Araştırma Makalesi
Determination of Oxidative Stress, Vitamin A, E, C and β-Carotene Levels in the Tissues of Luciobarbus esocinus (Heckel, 1843)
Öz
In the study was
investigated the levels
of lipid peroxidation (malondialdehyde;
MDA) as oxidative stress, vitamin E (VE), A (VA), C (VC) and β-carotene (βC) in
liver, spleen, muscle and kidney tissues of Luciobarbus esocinus. The fish samples used in this study was catched from
Keban Dam Lake (Elazıg-Turkey) at August. Analyzes were
made in High Performance Liquid Chromotograph (HPLC). The
data were analyzed with an Independent-Sample T Test in SPSS 21. It
was determined that the
levels of parameters analysed was generally higher in liver and spleen compared to
kidney and muscle of female and male. The VE, VC and βC levels in liver and kidney were lower in
female than male. The levels of VC in spleen were higher in male according to
female. The VA level in muscle was lower in male than female, but the level of βC in this tissues was higher in male according to
female. Moreover, the MDA level wasn’t different according to sex.
Anahtar Kelimeler
Kaynakça
- Barim, O., “The effects of dietary vitamin E on the oxidative stress and antioxidant enzyme activities in their tissues and ovarian egg numbers of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823)”, Journal of Animal and Veterinary Advances, 8(6),1190-1197, 2009.
- Ayala, A., Munoz, M.F. ve Argüelles, S., “Lipid Peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-Hydroxy-0-Nonenal”, Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity, Article ID 360438, http://dx.doi.org/10.1155/2014/360438., 2014.
- Regoli F. ve Giuliani ME., “Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organism”, Marine Environmental Research, 93, 106-117, 2014.
- Winston, G. W. ve Giulio, R. T. D., “Prooxidant and antioxidant mechanisms in aquatic organisms”, Aquatic Toxicology, 19, 137-161, 1991.
- Barim-Oz, O. ve Yılmaz, S., “Effects of dietary antioxidants on oxidative stress, antioxidant defence and growth of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823) during the reproductive period in females”, Aquaculture Research, 48, 2516-2527, 2017
- Beytut, E., Barim, Ö. Ve Kamiloglu, N.N., “Different levels of dietary DL-α tocopheryl acetate modulate the antioxidant defence system in the hepatopancreas, gills and muscles of the freshwater crayfish, Astacus leptodactylus (Eschscholtz, 1823)”, Journal of Animal and Veterinary Advances, 8(6), 1177-1184, 2009.
- Blahova, J., Plhalova, L., Hostovsky, M., Divisova, L., Dobsikova, R., Mikulikova, I., Stepanova, S. Ve Svobodova, Z.,“Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine”, Food and Chemical Toxicology, 61, 82-85, 2013.
- Palace, V. ve Werner, J., “Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review”, Scientia Marina, 70S2, 41-57, 2006.
- Barım Oz, O. Ve Sahin, H., “Oxidative stress and some biochemical parameters during starvation and refeeding in Astacus leptodactylus (Esch., 1823)”, Cellular and Molecular Biology, 62(13), 35-43, 2016.
- Furne M., Garcia-Galloge, M., Hidalgo, M.C., Morales, M.C., Do¬mezain, A., Domezain, J., Sanz, A.. “Oxidative stress parameters during starvation and refeeding periods in Adriatic Sturgeon (Acipenser naccarii) and rainbow trout (Oncorhynchusmykiss)”, Aquaculture Nutrition, 15, 587-595, 2009.
- Powell ,M.L., Watts, S.A., “Response to long-term nutrient depriva¬tion and recovery in the crayfish Procambarus clarkii and Procam¬barus zonangulus: oxygen consumption and metabolic enzymes”, Journal of the World Aquaculture Society, 41(1), 81-91, 2010.
- Cicek, D. ve Kumlu, M., “Effects of starvation periods on total lipit and fatty acid composition of green tiger shrimp (Penaeus semisul¬catus)”, Cukurova University Journal of Science and Engineering, 29(4), 95-104, 2013.
- Erişir, M., Barım, Ö., Özçelik, M. ve Harlıoğlu, M., “The effect of dietary vitamin E on the arginase activity in the females of freshwater crayfish (Asatacus leptodactylus, Esch. 1823)”, Turk J. Vet. Anim. Sci., 30, 195-199, 2006.
- Barim, O. ve Erisir, M., “The effect of dietary antioxidants an the argi¬nase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus Esch., 1823)”, Kafkas Univ J of the Fac of Vet Medic, 15, 745-750, 2009.
- Gjedrem, T., Robinson, N. ve Rye, M., “The importance of selective breeding in aquaculture to meet future demands for animal protein: A review”, Aquaculture, 350-353, 117-129, 2012.
- Anonim, “Keban Baraj Gölü’nün Limnolojik Raporu”, DSİ IX. Bölge Müdürlüğü, 137s, 1994.
- Geldiay, R. ve Balık, S., “Türkiye tatlı su balıkları (Ders Kitabı)”, V. Baskı, Ege Üniversitesi Su Ürünleri Fakültesi Yayınları, 46(16), 644s, Bornova-İzmir, 2007.
- Alpbaz, A., “Su ürünleri yetiştiriciliği”, Alp Yayınları, İzmir, 548s, 2005.
- Barım Oz, O. Ve Sahin, H., “The influence of dietary antioxidant on ovarian eggs and levels of vitamin E, C, A, astaxanthin, β-carotene and oxidatif stres in tissues of Astacus leptodactylus (Eschscholtz) during reproduction”, Cellular and Molecular Biology, 62(14), 1-10, 2016.
- Paital, B. ve Chainy, G.B.N., “Seasonal variability of antioxidant biomarkes in mud crabs (Scylla serrata)”, Ecotoxicology and Environmental Safety, 87, 33-41. Verlecar, X.N., Jena K.B. ve Chainy, G.B.N., “Seasonal variation of oxidative biomarkers in gills and digestive gland of green-lipped mussel Perna viridis from Arabian Sea. Estuarine”, Coastal and Shelf Science, 76, 745-752, 2008.
- Barim, Ö., Benzer, F., Erisir, M. ve Dörücü, M., “Oxidant and antioxidant status of tissues of freshwater crayfish (Astacus leptodactylus Esch., 1823) from different stations in the Keban Dam Lake”, Fresenius Environmental Bulletin, 18, 6s, 2009.
- Barim, Ö. ve Karatepe, M., “The effects of pollution on the vitamins A, E, C, β-carotene contens and oxidative stress of the freshwater crayfish, Astacus leptodactylus”, Ecotoxicology and Environmental Safety, 73, 138-142, 2010.
- Borkovic, S.S., Pavloviae, S.Z., Kovacevic, T.B., Stajn, A.S., Petroviae, V.M. ve Saieie, Z.S., “Antioxidant defence enzymre activities in hepatopancreas, gills and muscle of spiny cheek crayfish (Orconectes limosus) from the river danube”, Comparative Biochem. Physiol. Part C., 147(1), 122-128, 2007.
- Bayir, A., Sirkecioglu A.N., Haliloglu, H.I., Aksakal, E., Gunes, M. ve Aras, N.M., “Influence of season on antioxidant defense systems of Silurus glanis Linnaeus (Siluridae) and Barbus capito capito Güldenstädt (Cyprinidae)”, Fresen. Environ. Bull., 20(1), 3-11, 2011.
- Pisoschi, A.M. ve Pop, A., “The role of antioxidants in the chemistry of oxidative stres: A review”, Europan Journal of Medicinal Chemistry, 97, 55-74, 2015.
- Fang, T.-Z., Yang, S. ve Wu, G., “Free radicals, antioxidants, and nutrition”, Nutrition, 18, 872-879, 2002.
- Kiokias, S. ve Varzakaz, T., “Activity of flavonoids and β-carotene during the auto-oxidative deterioration of model food oil-in water emulsions”, Food Chemistry, 150, 280-286, 2014.
- Bell, J.G., McEvoy, J., Tocher D.R., ve Sargent, J.R., “Depletion of α-tocopherol and astaxanthin and Atlantic Salmon (Salmo salar) affects autoxidative defense and fatty acid metabolism”, The Journal of Nutrition, 130, 1800-1808, 2000.
- Cavalli, R.O., Batista, F.M.M., Lavens, P., Sorgeloos, P., Nelis, H.J. ve Leenheer, A.P.D., “Effects of Dietary Supplementation of vitamins C and E maternal performance and larval quality of the prawn Macrobrachium rosenbergii, Aquaculture, 227, 131-146, 2003.
- Arslan, A., Nazıroğlu, M., Gönülalan, Z., Sarıgöl, C. ve Aksakal, M., “Effects of various storage temperature and storage time on vitamin E levels of fish muscle”, Tr. J. of Veterinary and Animal Sciences, 21, 211-214, 1997.
- Köprücü, K. ve Özdemir, Y., “Gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum, 1972) etindeki A2, C ve E vitaminleri miktarları”, F.Ü. Fen ve Mühendislik Bilimleri Dergisi, 14(2), 227-233, 2002.
- Harlioğlu, M.M., Köprücü, K. ve Ozdemir, Y., “The effect of dietary vitamin E on the pleopodal egg number of Astacus leptodactylus (Eschscholtz, 1823)”, Aquaculture International, 10, 391-397, 2002.
- Nettleton J.A. ve Exler, J., “Nutrients in wild and farmed fish and shelfish”, Journal of Food Science, 57(2), 257-260, 1992.
Luciobarbus esocinus (Heckel, 1843)’un Dokularındaki Oksidatif Stress, Vitamin A, E, C ve β-Karoten SeviyelerininTespiti
Öz
Bu çalışmada Luciobarbus
esocinus’un karaciğer,
dalak, kas ve böbrek dokularında oksidatif stres olarak lipid peroksidasyon
(malondialdehit-MDA), vitamin
E (VE), A (VA), C (VC) ve β-karoten (βC) seviyeleri araştırıldı. Çalışmada
kullanılan balık örnekleri Ağustos ayında Keban Baraj Gölü’nden yakalandı.
Analizler yüksek performanslı likit kromotografisinde yapıldı (HPLC). Veriler
SPSS 21’deIndependent-Sample T and Duncan Testi’nde analiz
edildi. Genel olarak analiz yapılan parametrelerin düzeyleri erkek ve dişilerin
böbrek ve kasına göre karaciğer ve dalağında daha yüksekti. Karaciğer ve
böbrekteki VE, VC ve βC
seviyeleri erkeklere göre dişilerde daha düşüktü. Dalaktaki VC seviyesi dişiye
göre erkekte daha yüksekti. Kastaki VA seviyesi dişiye göre erkeklerde daha
düşüktü. Fakat bu dokudaki βC’nin seviyesi dişiye göre erkekte daha yüksekti.
Bunlara ilave olarak bütün dokulardaki MDA seviyesi cinsiyete göre farklılık
göstermedi.
Anahtar Kelimeler
Kaynakça
- Barim, O., “The effects of dietary vitamin E on the oxidative stress and antioxidant enzyme activities in their tissues and ovarian egg numbers of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823)”, Journal of Animal and Veterinary Advances, 8(6),1190-1197, 2009.
- Ayala, A., Munoz, M.F. ve Argüelles, S., “Lipid Peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-Hydroxy-0-Nonenal”, Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity, Article ID 360438, http://dx.doi.org/10.1155/2014/360438., 2014.
- Regoli F. ve Giuliani ME., “Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organism”, Marine Environmental Research, 93, 106-117, 2014.
- Winston, G. W. ve Giulio, R. T. D., “Prooxidant and antioxidant mechanisms in aquatic organisms”, Aquatic Toxicology, 19, 137-161, 1991.
- Barim-Oz, O. ve Yılmaz, S., “Effects of dietary antioxidants on oxidative stress, antioxidant defence and growth of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823) during the reproductive period in females”, Aquaculture Research, 48, 2516-2527, 2017
- Beytut, E., Barim, Ö. Ve Kamiloglu, N.N., “Different levels of dietary DL-α tocopheryl acetate modulate the antioxidant defence system in the hepatopancreas, gills and muscles of the freshwater crayfish, Astacus leptodactylus (Eschscholtz, 1823)”, Journal of Animal and Veterinary Advances, 8(6), 1177-1184, 2009.
- Blahova, J., Plhalova, L., Hostovsky, M., Divisova, L., Dobsikova, R., Mikulikova, I., Stepanova, S. Ve Svobodova, Z.,“Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine”, Food and Chemical Toxicology, 61, 82-85, 2013.
- Palace, V. ve Werner, J., “Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review”, Scientia Marina, 70S2, 41-57, 2006.
- Barım Oz, O. Ve Sahin, H., “Oxidative stress and some biochemical parameters during starvation and refeeding in Astacus leptodactylus (Esch., 1823)”, Cellular and Molecular Biology, 62(13), 35-43, 2016.
- Furne M., Garcia-Galloge, M., Hidalgo, M.C., Morales, M.C., Do¬mezain, A., Domezain, J., Sanz, A.. “Oxidative stress parameters during starvation and refeeding periods in Adriatic Sturgeon (Acipenser naccarii) and rainbow trout (Oncorhynchusmykiss)”, Aquaculture Nutrition, 15, 587-595, 2009.
- Powell ,M.L., Watts, S.A., “Response to long-term nutrient depriva¬tion and recovery in the crayfish Procambarus clarkii and Procam¬barus zonangulus: oxygen consumption and metabolic enzymes”, Journal of the World Aquaculture Society, 41(1), 81-91, 2010.
- Cicek, D. ve Kumlu, M., “Effects of starvation periods on total lipit and fatty acid composition of green tiger shrimp (Penaeus semisul¬catus)”, Cukurova University Journal of Science and Engineering, 29(4), 95-104, 2013.
- Erişir, M., Barım, Ö., Özçelik, M. ve Harlıoğlu, M., “The effect of dietary vitamin E on the arginase activity in the females of freshwater crayfish (Asatacus leptodactylus, Esch. 1823)”, Turk J. Vet. Anim. Sci., 30, 195-199, 2006.
- Barim, O. ve Erisir, M., “The effect of dietary antioxidants an the argi¬nase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus Esch., 1823)”, Kafkas Univ J of the Fac of Vet Medic, 15, 745-750, 2009.
- Gjedrem, T., Robinson, N. ve Rye, M., “The importance of selective breeding in aquaculture to meet future demands for animal protein: A review”, Aquaculture, 350-353, 117-129, 2012.
- Anonim, “Keban Baraj Gölü’nün Limnolojik Raporu”, DSİ IX. Bölge Müdürlüğü, 137s, 1994.
- Geldiay, R. ve Balık, S., “Türkiye tatlı su balıkları (Ders Kitabı)”, V. Baskı, Ege Üniversitesi Su Ürünleri Fakültesi Yayınları, 46(16), 644s, Bornova-İzmir, 2007.
- Alpbaz, A., “Su ürünleri yetiştiriciliği”, Alp Yayınları, İzmir, 548s, 2005.
- Barım Oz, O. Ve Sahin, H., “The influence of dietary antioxidant on ovarian eggs and levels of vitamin E, C, A, astaxanthin, β-carotene and oxidatif stres in tissues of Astacus leptodactylus (Eschscholtz) during reproduction”, Cellular and Molecular Biology, 62(14), 1-10, 2016.
- Paital, B. ve Chainy, G.B.N., “Seasonal variability of antioxidant biomarkes in mud crabs (Scylla serrata)”, Ecotoxicology and Environmental Safety, 87, 33-41. Verlecar, X.N., Jena K.B. ve Chainy, G.B.N., “Seasonal variation of oxidative biomarkers in gills and digestive gland of green-lipped mussel Perna viridis from Arabian Sea. Estuarine”, Coastal and Shelf Science, 76, 745-752, 2008.
- Barim, Ö., Benzer, F., Erisir, M. ve Dörücü, M., “Oxidant and antioxidant status of tissues of freshwater crayfish (Astacus leptodactylus Esch., 1823) from different stations in the Keban Dam Lake”, Fresenius Environmental Bulletin, 18, 6s, 2009.
- Barim, Ö. ve Karatepe, M., “The effects of pollution on the vitamins A, E, C, β-carotene contens and oxidative stress of the freshwater crayfish, Astacus leptodactylus”, Ecotoxicology and Environmental Safety, 73, 138-142, 2010.
- Borkovic, S.S., Pavloviae, S.Z., Kovacevic, T.B., Stajn, A.S., Petroviae, V.M. ve Saieie, Z.S., “Antioxidant defence enzymre activities in hepatopancreas, gills and muscle of spiny cheek crayfish (Orconectes limosus) from the river danube”, Comparative Biochem. Physiol. Part C., 147(1), 122-128, 2007.
- Bayir, A., Sirkecioglu A.N., Haliloglu, H.I., Aksakal, E., Gunes, M. ve Aras, N.M., “Influence of season on antioxidant defense systems of Silurus glanis Linnaeus (Siluridae) and Barbus capito capito Güldenstädt (Cyprinidae)”, Fresen. Environ. Bull., 20(1), 3-11, 2011.
- Pisoschi, A.M. ve Pop, A., “The role of antioxidants in the chemistry of oxidative stres: A review”, Europan Journal of Medicinal Chemistry, 97, 55-74, 2015.
- Fang, T.-Z., Yang, S. ve Wu, G., “Free radicals, antioxidants, and nutrition”, Nutrition, 18, 872-879, 2002.
- Kiokias, S. ve Varzakaz, T., “Activity of flavonoids and β-carotene during the auto-oxidative deterioration of model food oil-in water emulsions”, Food Chemistry, 150, 280-286, 2014.
- Bell, J.G., McEvoy, J., Tocher D.R., ve Sargent, J.R., “Depletion of α-tocopherol and astaxanthin and Atlantic Salmon (Salmo salar) affects autoxidative defense and fatty acid metabolism”, The Journal of Nutrition, 130, 1800-1808, 2000.
- Cavalli, R.O., Batista, F.M.M., Lavens, P., Sorgeloos, P., Nelis, H.J. ve Leenheer, A.P.D., “Effects of Dietary Supplementation of vitamins C and E maternal performance and larval quality of the prawn Macrobrachium rosenbergii, Aquaculture, 227, 131-146, 2003.
- Arslan, A., Nazıroğlu, M., Gönülalan, Z., Sarıgöl, C. ve Aksakal, M., “Effects of various storage temperature and storage time on vitamin E levels of fish muscle”, Tr. J. of Veterinary and Animal Sciences, 21, 211-214, 1997.
- Köprücü, K. ve Özdemir, Y., “Gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum, 1972) etindeki A2, C ve E vitaminleri miktarları”, F.Ü. Fen ve Mühendislik Bilimleri Dergisi, 14(2), 227-233, 2002.
- Harlioğlu, M.M., Köprücü, K. ve Ozdemir, Y., “The effect of dietary vitamin E on the pleopodal egg number of Astacus leptodactylus (Eschscholtz, 1823)”, Aquaculture International, 10, 391-397, 2002.
- Nettleton J.A. ve Exler, J., “Nutrients in wild and farmed fish and shelfish”, Journal of Food Science, 57(2), 257-260, 1992.
Toplam 33 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Mühendislik |
| Bölüm | Biyoloji |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Aralık 2017 |
| Kabul Tarihi | 7 Aralık 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 6 Sayı: 2 |
Kaynak Göster
Dergimizin tarandığı indeksler