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Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis

Yıl 2023, Cilt: 13 Sayı: 01, 38 - 46, 15.03.2023

Melih Enes Altınok Mustafa Ture Ayse Cebeci Ilhan Altınok

https://doi.org/10.5799/jmid.1265400

Öz

Objectives: Salmonella Enteritidis is a pathogen that causes gastroenteritis and septicemia in humans and animals, causing worldwide health implications. This pathogen often causes fatal foodborne diseases in humans and animals. In recent years, new treatment strategies for bacterial infections have been studied. Bacteriophages might be used as an alternative strategy for battling against bacterial diseases.
Methods: A novel SP-T1 phage was isolated, and its lytic activity against Salmonella Enteritidis strains and other enteric bacterial species was evaluated. In addition to its kinetic and genetic characteristics, it is in vivo cytotoxicity and ability to inhibit the development of biofilms were investigated.
Results: The gene sequence of the major capsid protein confirmed that the SP-T1 phage belonged to the family Siphoviridae. There was no evidence that phage had a lytic effect on bacteria other than the type of Salmonella used in this investigation. The phage exhibited a short latent period of 15 to 20 min and a burst size of 10 plaque forming unit per infected cell. The phage was resistant to temperatures between 15 and 37 °C and pH levels between 5 and 11. The SP-T1 phage exhibited no adverse effects on EPC cell culture and reduced biofilm formation.
Conclusions: Because of its short latent period, appropriate burst size, ability to inhibit biofilm formation, and sensitivity to various temperatures and pH levels, the phage found in this study would be an excellent candidate for phage therapy. These findings are beneficial for improving the microbiological safety and quality of food and creating efficient bacteriophage-based regulation in the food chain. J Microbiol Infect Dis 2023; 12(4):38-46.

Anahtar Kelimeler

Biofilm formation Lytic bacteriophages Salmonella enterica serovar Enteritidis

Yıl 2023, Cilt: 13 Sayı: 01, 38 - 46, 15.03.2023

Melih Enes Altınok Mustafa Ture Ayse Cebeci Ilhan Altınok

https://doi.org/10.5799/jmid.1265400

Öz

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Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Research Article
Yazarlar

Melih Enes Altınok Bu kişi benim

Mustafa Ture

Ayse Cebeci Bu kişi benim

Ilhan Altınok Bu kişi benim

Yayımlanma Tarihi 15 Mart 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 13 Sayı: 01

Kaynak Göster

APA Altınok, M. E., Ture, M., Cebeci, A., Altınok, I. (2023). Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis. Journal of Microbiology and Infectious Diseases, 13(01), 38-46. https://doi.org/10.5799/jmid.1265400
AMA Altınok ME, Ture M, Cebeci A, Altınok I. Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis. J Microbil Infect Dis. Mart 2023;13(01):38-46. doi:10.5799/jmid.1265400
Chicago Altınok, Melih Enes, Mustafa Ture, Ayse Cebeci, ve Ilhan Altınok. “Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis”. Journal of Microbiology and Infectious Diseases 13, sy. 01 (Mart 2023): 38-46. https://doi.org/10.5799/jmid.1265400.
EndNote Altınok ME, Ture M, Cebeci A, Altınok I (01 Mart 2023) Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis. Journal of Microbiology and Infectious Diseases 13 01 38–46.
IEEE M. E. Altınok, M. Ture, A. Cebeci, ve I. Altınok, “Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis”, J Microbil Infect Dis, c. 13, sy. 01, ss. 38–46, 2023, doi: 10.5799/jmid.1265400.
ISNAD Altınok, Melih Enes vd. “Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis”. Journal of Microbiology and Infectious Diseases 13/01 (Mart 2023), 38-46. https://doi.org/10.5799/jmid.1265400.
JAMA Altınok ME, Ture M, Cebeci A, Altınok I. Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis. J Microbil Infect Dis. 2023;13:38–46.
MLA Altınok, Melih Enes vd. “Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis”. Journal of Microbiology and Infectious Diseases, c. 13, sy. 01, 2023, ss. 38-46, doi:10.5799/jmid.1265400.
Vancouver Altınok ME, Ture M, Cebeci A, Altınok I. Characterization of SP-T1 Phage for Potential Biological Control of Salmonella Enteritidis. J Microbil Infect Dis. 2023;13(01):38-46.