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The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes

Yıl 2020, Cilt: 10 Sayı: 3, 1811 - 1819, 01.09.2020
https://doi.org/10.21597/jist.688416

Öz

Carbonic anhydrases are vital class of enzymes that participate in so many essential physiological events in the organism and associated with many diseases. Inhibitors of carbonic anhydrase enzymes are used in pharmacological applications in many areas such as antiobesity, antiglaucoma, anticancer agents and anticonvulsants. So, this study focuses on the characterization of CA-I and CA-II isoenzymes purified from sheep stomach and investigating the inhibition effects of antibiotics on these enzymes. The findings show that the antibiotics studied strongly inhibit CA-I and CA-II isozymes. In our presented study, using Sepharose 4B-L-Tyrosine sulfanilamide affinity gel chromatography, carbonic anhydrase I and carbonic anhydrase II isoenzymes were purified from sheep stomach with a yield of 51.9%, 78%, respectively and specific activity of CA I and CA II are 4864.8 EU/mg and 5652.02 EU/mg, respectively. The overall purifications from sheep stomach CA I and CA II were approximately 529.4 and 615.2 fold respectively. To check the purify of the enzymes, it was conducted an electrophoretic method so-called Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and single bands were detected for both enzymes. Additionally, the effects of antibiotics on sheep stomach CA I and CA II isozymes activities, using both hydratase and esterase activity methods, were researched. IC50 values of enrofloxacin, tylosin and ampicillin antibiotics that inhibit CA I enzyme with hydratase activity were found as 1.41, 0.033 and 1.56 mM, respectively. IC50 values for CA II enzyme with hydratase activity of the same antibiotics were found as 2.47, 0.039 and 1.63 mM, respectively. Kanamycin and amikacin antibiotics inhibited both CA I and CA II enzymes with esterase activity. IC50 values for CA I and CA II enzymes with esterase activity of kanamycin antibiotic were determined as 0.0488 and 0.118 mM, respectively. IC50 values of amikacin for CA I and CA II enzymes with esterase activity were determined as 0.0163 and 0.036 mM, respectively.

Kaynakça

  • Alım Z, 2019. Inhibition Effect of Eosin Y on Carbonic Anhydrase (CA) I and II Isoenzymes Purified from Human Erythrocytes. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(1): 338-344.
  • Alım Z, Kılınç N, İşgör MM, Şengül B, Beydemir Ş, 2015. Some Anti‐Inflammatory Agents Inhibit Esterase Activities of Human Carbonic Anhydrase Isoforms I and II: An In Vitro Study. Chemical Biology & Drug Design, 86(4): 857-863.
  • Armstrong JM, Myers DV, Verpoorte JA, Edsall JT, 1966. Purification and Properties of Human Erythrocyte Carbonic Anhydrases. Journal of biological chemistry, 241(21): 5137-5149.
  • Baguer AJ, Jensen J, Krogh PH, 2000. Effects of the Antibiotics Oxytetracycline and Tylosin on Soil Fauna. Chemosphere, 40(7): 751-757.
  • Beydemir Ş, Çiftçi M, Küfrevioğlu Öİ, 2002. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase from Sheep Erythrocytes and Inhibitory Effects of Some Antibiotics on Enzyme Activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 17(4): 271-277.
  • Bradford MM, 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72(1-2): 248-254.
  • Caglayan C, Demir Y, Kucukler S, Taslimi P, Kandemir FM, Gulçin İ, 2019. The effects of hesperidin on sodium arsenite‐induced different organ toxicity in rats on metabolic enzymes as antidiabetic and anticholinergics potentials: A biochemical approach. Journal of food biochemistry, 43(2), e12720
  • Chegwidden WR, Spencer IM, 1996. Carbonic Anhydrase Provides Bicarbonate for De Novo Lipogenesis in the Locust. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 115(2): 247-254.
  • Chiche J, Ilc K, Laferrière J, Trottier E, Dayan F, Mazure NM, Brahimi-Horn MC, Pouysségur J, 2009. Hypoxia-Inducible Carbonic Anhydrase IX and XII Promote Tumor Cell Growth by Counteracting Acidosis Through the Regulation of the Intracellular pH. Cancer Research, 69(1): 358-368.
  • Dimitrova DJ, Lashev LD, Yanev SG, Pandova B, 2007. Pharmacokinetics of Enrofloxacin in Turkeys. Research in Veterinary Science, 82(3): 392-397.
  • Dodgson SJ, 1987. Inhibition of Mitochondrial Carbonic Anhydrase and Ureagenesis: A Discrepancy Examined. Journal of Applied Physiology, 63(5): 2134-2141.
  • Dodgson SJ, Cherian K, 1989. Mitochondrial Carbonic Anhydrase is Involved in Rat Renal Glucose Synthesis. American Journal of Physiology-Endocrinology and Metabolism, 257(6): 791-796.
  • Geers C, Gros G, 2000. Carbon Dioxide Transport and Carbonic Anhydrase in Blood and Muscle. Physiological Reviews, 80(2): 681-715.
  • Gündoğdu S, Türkeş C, Arslan M, Demir Y, Beydemir Ş, 2019. New Isoindole‐1, 3‐dione Substituted Sulfonamides as Potent Inhibitors of Carbonic Anhydrase and Acetylcholinesterase: Design, Synthesis, and Biological Evaluation. Chemistry Select, 4(45): 13347-13355.
  • Kaya ED, Söyüt H, Beydemir Ş, 2013. Carbonic Anhydrase Activity from the Gilthead Sea Bream (Sparus Aurata) Liver: The Toxicological Effects of Heavy Metals. Environmental Toxicology and Pharmacology, 36(2): 514-521.
  • Kılınç N, Alım Z, İşgör MM, Beydemir Ş, 2014. The Impacts of Some Metals on the Activity of Corb Gill Umbrina Cirrosa Carbonic Anhydrase. Hacettepe Journal of Biology & Chemistry, 42 (4): 499-504.
  • Kılınç N, İşgör MM, Şengül B, Beydemir Ş, 2015. Influence of Pesticide Exposure on Carbonic Anhydrase II from Sheep Stomach. Toxicology and Industrial Health, 31(9): 823-830.
  • Kılınç N, Alım Z, Şengül B, İşgör MM, Beydemir Ş, 2018. Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 7(1): 90-97.
  • Laemlli DK, 1970. Cleavege of Structural Ptoteins During in Assembly of The head of Bacteriophage. Nature, 227-680.
  • Liu F, Martin-Mingot A, Lecornué F, Jouannetaud MP, Maresca A, Thibaudeau S, Supuran CT, 2012. Carbonic Anhydrases Inhibitory Effects of New Benzenesulfonamides Synthesized by Using Superacid Chemistry. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(6): 886-891.
  • Oertel R, Neumeister V, Kirch W, 2004. Hydrophilic Interaction Chromatography Combined with Tandem-Mass Spectrometry to Determine Six Aminoglycosides in Serum. Journal of Chromatography A, 1058(1-2), 197-201.
  • Özensoy Ö, Arslan M, Supuran CT, 2011. Carbonic Anhydrase Inhibitors: Purification and Inhibition Studies of Pigeon (Columba Livia Var. Domestica) Red Blood Cell Carbonic Anhydrase with Sulfonamides. Journal of Enzyme Inhibition and Medicinal Chemistry, 26(5): 749-753.
  • Parkkila S, Lasota J, Fletcher JA, Ou WB, Kivelä AJ, Nuorva K, Parkkila AK, Ollikainen J, Sly WS, Waheed A, Pastorekova S, Pastorek J, Isola J, Miettinen M, 2010. Carbonic Anhydrase II. A Novel Biomarker for Gastrointestinal Stromal Tumors. Modern Pathology, 23(5): 743.
  • Potter CPS, Harris AL, 2003. Diagnostic, Prognostic and Therapeutic Implications of Carbonic Anhydrases in Cancer. British Journal of Cancer, 89(1): 2.
  • Rafailidis PI, Ioannidou EN, Falagas ME, 2007. Ampicillin/Sulbactam. Drugs, 67(13): 1829-1849.
  • Sahin H, Aliyazicioglu R, Yildiz O, Kolayli S, Innocenti A, Supuran CT, 2011. Honey, Polen, and Propolis Extracts Show Potent Inhibitory Activity Against the Zinc Metalloenzyme Carbonic Anhydrase. Journal of Enzyme Inhibition and Medicinal Chemistry, 26(3): 440-444.
  • Sahin H, Can Z, Yildiz O, Kolayli S, Innocenti A, Scozzafava G, Supuran CT, 2012. Inhibition of Carbonic Anhydrase Isozymes I and II with Natural Products Extracted from Plants, Mushrooms and Honey. Journal Of Enzyme Inhibition and Medicinal Chemistry, 27(3): 395-402.
  • Sly WS, Hu PY, 1995. Human Carbonic Anhydrases and Carbonic Anhydrase Deficiencies. Annual Review of Biochemistry, 64(1): 375-401.
  • Spencer IM, Hargreaves I, Chegwidden WR, 1988. Effect of the Carbonic Anhydrase Inhibitor Acetazolamide on Lipid Synthesis in the Locust. Biochemical Society Transactions, 16: 973-974.
  • Supuran CT, 2008. Carbonic Anhydrases: Novel Therapeutic Applications for Inhibitors and Activators. Nature Reviews Drug Discovery, 7(2): 168.
  • Supuran CT, 2010. Carbonic Anhydrase Inhibitors. Bioorganic and Medicinal Chemistry Letters. 20(12): 3467–3474
  • Supuran CT, 2012. Structure-Based Drug Discovery of Carbonic Anhydrase Inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(6): 759-772.
  • Supuran CT, Scozzafava A, Casini A, 2003. Carbonic Anhydrase Inhibitors. Medicinal Research Reviews, 23(2): 146-189.
  • Şentürk M, Ekinci D, Göksu S, Supuran CT, 2012. Effects of Dopaminergic Compounds on Carbonic Anhydrase Isozymes I, II, and VI. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(3): 365-369.
  • Verpoorte JA, Mehta S, Edsall JT, 1967. Esterase Activities of Human Carbonic Anhydrases B and C. Journal of Biological Chemistry, 242(18): 4221-4229.
  • Wilbur KM, Anderson NG, 1948. Electrometric and Colorimetric Determination of Carbonic Anhydrase. Journal of Biological Chemistry, 176(1): 147-154.
  • Zhanel GG, Lawson CD, Zelenitsky S, Findlay B, Schweizer F, Adam H, Walkty A, Rubinstein E, Gin AS, Hoban DJ, Lynch JP, Karlowsky JA, 2012. Comparison of the Next-Generation Aminoglycoside Plazomicin to Gentamicin, Tobramycin and Amikacin. Expert Review of Anti-Infective Therapy, 10(4), 459-473.

The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes

Yıl 2020, Cilt: 10 Sayı: 3, 1811 - 1819, 01.09.2020
https://doi.org/10.21597/jist.688416

Öz

Carbonic anhydrases are vital class of enzymes that participate in so many essential physiological events in the organism and associated with many diseases. Inhibitors of carbonic anhydrase enzymes are used in pharmacological applications in many areas such as antiobesity, antiglaucoma, anticancer agents and anticonvulsants. So, this study focuses on the characterization of CA-I and CA-II isoenzymes purified from sheep stomach and investigating the inhibition effects of antibiotics on these enzymes. The findings show that the antibiotics studied strongly inhibit CA-I and CA-II isozymes. In our presented study, using Sepharose 4B-L-Tyrosine sulfanilamide affinity gel chromatography, carbonic anhydrase I and carbonic anhydrase II isoenzymes were purified from sheep stomach with a yield of 51.9%, 78%, respectively and specific activity of CA I and CA II are 4864.8 EU/mg and 5652.02 EU/mg, respectively. The overall purifications from sheep stomach CA I and CA II were approximately 529.4 and 615.2 fold respectively. To check the purify of the enzymes, it was conducted an electrophoretic method so-called Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and single bands were detected for both enzymes. Additionally, the effects of antibiotics on sheep stomach CA I and CA II isozymes activities, using both hydratase and esterase activity methods, were researched. IC50 values of enrofloxacin, tylosin and ampicillin antibiotics that inhibit CA I enzyme with hydratase activity were found as 1.41, 0.033 and 1.56 mM, respectively. IC50 values for CA II enzyme with hydratase activity of the same antibiotics were found as 2.47, 0.039 and 1.63 mM, respectively. Kanamycin and amikacin antibiotics inhibited both CA I and CA II enzymes with esterase activity. IC50 values for CA I and CA II enzymes with esterase activity of kanamycin antibiotic were determined as 0.0488 and 0.118 mM, respectively. IC50 values of amikacin for CA I and CA II enzymes with esterase activity were determined as 0.0163 and 0.036 mM, respectively.

Kaynakça

  • Alım Z, 2019. Inhibition Effect of Eosin Y on Carbonic Anhydrase (CA) I and II Isoenzymes Purified from Human Erythrocytes. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(1): 338-344.
  • Alım Z, Kılınç N, İşgör MM, Şengül B, Beydemir Ş, 2015. Some Anti‐Inflammatory Agents Inhibit Esterase Activities of Human Carbonic Anhydrase Isoforms I and II: An In Vitro Study. Chemical Biology & Drug Design, 86(4): 857-863.
  • Armstrong JM, Myers DV, Verpoorte JA, Edsall JT, 1966. Purification and Properties of Human Erythrocyte Carbonic Anhydrases. Journal of biological chemistry, 241(21): 5137-5149.
  • Baguer AJ, Jensen J, Krogh PH, 2000. Effects of the Antibiotics Oxytetracycline and Tylosin on Soil Fauna. Chemosphere, 40(7): 751-757.
  • Beydemir Ş, Çiftçi M, Küfrevioğlu Öİ, 2002. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase from Sheep Erythrocytes and Inhibitory Effects of Some Antibiotics on Enzyme Activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 17(4): 271-277.
  • Bradford MM, 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72(1-2): 248-254.
  • Caglayan C, Demir Y, Kucukler S, Taslimi P, Kandemir FM, Gulçin İ, 2019. The effects of hesperidin on sodium arsenite‐induced different organ toxicity in rats on metabolic enzymes as antidiabetic and anticholinergics potentials: A biochemical approach. Journal of food biochemistry, 43(2), e12720
  • Chegwidden WR, Spencer IM, 1996. Carbonic Anhydrase Provides Bicarbonate for De Novo Lipogenesis in the Locust. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 115(2): 247-254.
  • Chiche J, Ilc K, Laferrière J, Trottier E, Dayan F, Mazure NM, Brahimi-Horn MC, Pouysségur J, 2009. Hypoxia-Inducible Carbonic Anhydrase IX and XII Promote Tumor Cell Growth by Counteracting Acidosis Through the Regulation of the Intracellular pH. Cancer Research, 69(1): 358-368.
  • Dimitrova DJ, Lashev LD, Yanev SG, Pandova B, 2007. Pharmacokinetics of Enrofloxacin in Turkeys. Research in Veterinary Science, 82(3): 392-397.
  • Dodgson SJ, 1987. Inhibition of Mitochondrial Carbonic Anhydrase and Ureagenesis: A Discrepancy Examined. Journal of Applied Physiology, 63(5): 2134-2141.
  • Dodgson SJ, Cherian K, 1989. Mitochondrial Carbonic Anhydrase is Involved in Rat Renal Glucose Synthesis. American Journal of Physiology-Endocrinology and Metabolism, 257(6): 791-796.
  • Geers C, Gros G, 2000. Carbon Dioxide Transport and Carbonic Anhydrase in Blood and Muscle. Physiological Reviews, 80(2): 681-715.
  • Gündoğdu S, Türkeş C, Arslan M, Demir Y, Beydemir Ş, 2019. New Isoindole‐1, 3‐dione Substituted Sulfonamides as Potent Inhibitors of Carbonic Anhydrase and Acetylcholinesterase: Design, Synthesis, and Biological Evaluation. Chemistry Select, 4(45): 13347-13355.
  • Kaya ED, Söyüt H, Beydemir Ş, 2013. Carbonic Anhydrase Activity from the Gilthead Sea Bream (Sparus Aurata) Liver: The Toxicological Effects of Heavy Metals. Environmental Toxicology and Pharmacology, 36(2): 514-521.
  • Kılınç N, Alım Z, İşgör MM, Beydemir Ş, 2014. The Impacts of Some Metals on the Activity of Corb Gill Umbrina Cirrosa Carbonic Anhydrase. Hacettepe Journal of Biology & Chemistry, 42 (4): 499-504.
  • Kılınç N, İşgör MM, Şengül B, Beydemir Ş, 2015. Influence of Pesticide Exposure on Carbonic Anhydrase II from Sheep Stomach. Toxicology and Industrial Health, 31(9): 823-830.
  • Kılınç N, Alım Z, Şengül B, İşgör MM, Beydemir Ş, 2018. Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 7(1): 90-97.
  • Laemlli DK, 1970. Cleavege of Structural Ptoteins During in Assembly of The head of Bacteriophage. Nature, 227-680.
  • Liu F, Martin-Mingot A, Lecornué F, Jouannetaud MP, Maresca A, Thibaudeau S, Supuran CT, 2012. Carbonic Anhydrases Inhibitory Effects of New Benzenesulfonamides Synthesized by Using Superacid Chemistry. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(6): 886-891.
  • Oertel R, Neumeister V, Kirch W, 2004. Hydrophilic Interaction Chromatography Combined with Tandem-Mass Spectrometry to Determine Six Aminoglycosides in Serum. Journal of Chromatography A, 1058(1-2), 197-201.
  • Özensoy Ö, Arslan M, Supuran CT, 2011. Carbonic Anhydrase Inhibitors: Purification and Inhibition Studies of Pigeon (Columba Livia Var. Domestica) Red Blood Cell Carbonic Anhydrase with Sulfonamides. Journal of Enzyme Inhibition and Medicinal Chemistry, 26(5): 749-753.
  • Parkkila S, Lasota J, Fletcher JA, Ou WB, Kivelä AJ, Nuorva K, Parkkila AK, Ollikainen J, Sly WS, Waheed A, Pastorekova S, Pastorek J, Isola J, Miettinen M, 2010. Carbonic Anhydrase II. A Novel Biomarker for Gastrointestinal Stromal Tumors. Modern Pathology, 23(5): 743.
  • Potter CPS, Harris AL, 2003. Diagnostic, Prognostic and Therapeutic Implications of Carbonic Anhydrases in Cancer. British Journal of Cancer, 89(1): 2.
  • Rafailidis PI, Ioannidou EN, Falagas ME, 2007. Ampicillin/Sulbactam. Drugs, 67(13): 1829-1849.
  • Sahin H, Aliyazicioglu R, Yildiz O, Kolayli S, Innocenti A, Supuran CT, 2011. Honey, Polen, and Propolis Extracts Show Potent Inhibitory Activity Against the Zinc Metalloenzyme Carbonic Anhydrase. Journal of Enzyme Inhibition and Medicinal Chemistry, 26(3): 440-444.
  • Sahin H, Can Z, Yildiz O, Kolayli S, Innocenti A, Scozzafava G, Supuran CT, 2012. Inhibition of Carbonic Anhydrase Isozymes I and II with Natural Products Extracted from Plants, Mushrooms and Honey. Journal Of Enzyme Inhibition and Medicinal Chemistry, 27(3): 395-402.
  • Sly WS, Hu PY, 1995. Human Carbonic Anhydrases and Carbonic Anhydrase Deficiencies. Annual Review of Biochemistry, 64(1): 375-401.
  • Spencer IM, Hargreaves I, Chegwidden WR, 1988. Effect of the Carbonic Anhydrase Inhibitor Acetazolamide on Lipid Synthesis in the Locust. Biochemical Society Transactions, 16: 973-974.
  • Supuran CT, 2008. Carbonic Anhydrases: Novel Therapeutic Applications for Inhibitors and Activators. Nature Reviews Drug Discovery, 7(2): 168.
  • Supuran CT, 2010. Carbonic Anhydrase Inhibitors. Bioorganic and Medicinal Chemistry Letters. 20(12): 3467–3474
  • Supuran CT, 2012. Structure-Based Drug Discovery of Carbonic Anhydrase Inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(6): 759-772.
  • Supuran CT, Scozzafava A, Casini A, 2003. Carbonic Anhydrase Inhibitors. Medicinal Research Reviews, 23(2): 146-189.
  • Şentürk M, Ekinci D, Göksu S, Supuran CT, 2012. Effects of Dopaminergic Compounds on Carbonic Anhydrase Isozymes I, II, and VI. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(3): 365-369.
  • Verpoorte JA, Mehta S, Edsall JT, 1967. Esterase Activities of Human Carbonic Anhydrases B and C. Journal of Biological Chemistry, 242(18): 4221-4229.
  • Wilbur KM, Anderson NG, 1948. Electrometric and Colorimetric Determination of Carbonic Anhydrase. Journal of Biological Chemistry, 176(1): 147-154.
  • Zhanel GG, Lawson CD, Zelenitsky S, Findlay B, Schweizer F, Adam H, Walkty A, Rubinstein E, Gin AS, Hoban DJ, Lynch JP, Karlowsky JA, 2012. Comparison of the Next-Generation Aminoglycoside Plazomicin to Gentamicin, Tobramycin and Amikacin. Expert Review of Anti-Infective Therapy, 10(4), 459-473.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Kimya Mühendisliği
Bölüm Kimya / Chemistry
Yazarlar

Namık Kılınç 0000-0002-9102-1370

Şükrü Beydemir 0000-0003-3667-6902

Yayımlanma Tarihi 1 Eylül 2020
Gönderilme Tarihi 12 Şubat 2020
Kabul Tarihi 28 Nisan 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 10 Sayı: 3

Kaynak Göster

APA Kılınç, N., & Beydemir, Ş. (2020). The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes. Journal of the Institute of Science and Technology, 10(3), 1811-1819. https://doi.org/10.21597/jist.688416
AMA Kılınç N, Beydemir Ş. The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes. Iğdır Üniv. Fen Bil Enst. Der. Eylül 2020;10(3):1811-1819. doi:10.21597/jist.688416
Chicago Kılınç, Namık, ve Şükrü Beydemir. “The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes”. Journal of the Institute of Science and Technology 10, sy. 3 (Eylül 2020): 1811-19. https://doi.org/10.21597/jist.688416.
EndNote Kılınç N, Beydemir Ş (01 Eylül 2020) The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes. Journal of the Institute of Science and Technology 10 3 1811–1819.
IEEE N. Kılınç ve Ş. Beydemir, “The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes”, Iğdır Üniv. Fen Bil Enst. Der., c. 10, sy. 3, ss. 1811–1819, 2020, doi: 10.21597/jist.688416.
ISNAD Kılınç, Namık - Beydemir, Şükrü. “The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes”. Journal of the Institute of Science and Technology 10/3 (Eylül 2020), 1811-1819. https://doi.org/10.21597/jist.688416.
JAMA Kılınç N, Beydemir Ş. The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes. Iğdır Üniv. Fen Bil Enst. Der. 2020;10:1811–1819.
MLA Kılınç, Namık ve Şükrü Beydemir. “The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes”. Journal of the Institute of Science and Technology, c. 10, sy. 3, 2020, ss. 1811-9, doi:10.21597/jist.688416.
Vancouver Kılınç N, Beydemir Ş. The Evaluation of Inhibition Effects of Antibiotics on the Sheep Stomach Carbonic Anhydrase I and II Isozymes. Iğdır Üniv. Fen Bil Enst. Der. 2020;10(3):1811-9.