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OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ

Yıl 2023, Cilt: 47 Sayı: 2, 543 - 556, 20.05.2023
https://doi.org/10.33483/jfpau.1246570

Öz

Amaç: L929 fibroblast hücrelerinde H2O2 etkisiyle oluşturulan hücre hasarının bertaraf edilmesinde oksiresveratrolün rejeneratif etkisinin değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntem: Oksiresveratrolün total antioksidan seviyesi (TAS), total oksidan seviyesi (TOS), oksidatif stres indeksi (OSİ), toplam fenolik madde seviyeleri farklı derişimlerde ölçülmüştür. Oksiresveratrol fibroblast hücrelerinde IC50 değeri MTT yöntemiyle belirlenmesinin ardından 12.5 - 400 µM konsantrasyonlarında H2O2 ile meydana getirilen oksidatif stres hasarındaki rejeneratif etkisi in-vitro ortamdaki hücre proliferasyonunun ölçümü xCELLigence cihazıyla gerçekleştirilmiştir. Ayrıca hücre hasarındaki yara iyileşme düzeylerini belirlemek amacıyla 3.125 - 25 µM derişimlerinde scratch analizi yapılmıştır.
Sonuç ve Tartışma: Oksiresveratrolün 0.5 mg/ml’de TAS değeri 3 ± 0.3 ve TOS değeri 0.77 ± 0.52; (OSİ) değeri 0.02 ± 0.09 olarak bulunmuştur. Oksiresveratrolün 0.625 mg/ml’den 10 mg/ml’ye farklı dozlarındaki total fenolik madde (TFM) derişimleri düşük konsantrasyondan yüksek konsantrasyona daha yüksek TFM’ye sahip olduğu gözlemlenmiştir. Oksiresveratrolün L929 fibroblast hücreleri IC50 değeri 214.2 μM; H2O2’e maruz bırakılan L929 fibroblast hücrelerindeki hücre hattında oksiresveratrol IC50 değeri 109.7 μM olduğu belirlenmiştir. Oksiresveratrolün 12.5 μM konsantrasyonunun xCELLigence cihazıyla ölçülmesinde en fazla hücre proliferasyonu gözlenmiştir. Scratch analizinde H2O2 ile hasarlanmış hücrelerdeki 12.5 μM oksiresveratrol konsantrasyonunda 24. Saat için %62, 48. Saat için %88’lik bir hücre proliferasyonuyla diğer dozlardan daha fazla iyileşme gözlenmiştir. 12.5 μM konsantrasyonu hem proliferasyon, hem de scratch analizinde en etkili konsantrasyon olduğu belirlenmiştir. Oksiresveratrol; düşük konsantrasyonlarda gösterdiği antioksidan kapasiteyle H2O2 aracılığıyla meydana getirilen hücresel hasarda, L929 fibroblast hücrelerini oksidatif stresten korur, fibroblast canlılığına ve migrasyona bir güç çarpanı etkisi oluşturur. Bu sonuçlar oksiresveratrolün; serbest oksijen radikallerinin oluşturduğu akut ya da kronik hastalıkların engellenmesinde hücre düzeyinde etkili olabileceğini göstermiştir. Hücre düzeyinde gerçekleştirilecek daha kapsamlı çalışmalar, in-vivo çalışmalarla oksidatif stres kaynaklı hastalıkların tedavisinde oksiresveratrolün potansiyel bir molekül olabileceği düşünülebilir.

Kaynakça

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POSITIVE EFFECTS OF OXYRESVERATROL ON OXIDATIVE STRESS DAMAGE IN L929 FIBROBLAST CELLS

Yıl 2023, Cilt: 47 Sayı: 2, 543 - 556, 20.05.2023
https://doi.org/10.33483/jfpau.1246570

Öz

Objective: It was aimed to determine the regenerative effect of oxyresveratrol in eliminating the cell damage caused by the effect of H2O2 in L929 fibroblast cells.
Material and Method: Total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), total phenolic substance levels of oxyresveratrol were measured at different concentrations. After the IC50 value of oxyresveratrol fibroblast cells was determined by the MTT method, the regenerative effect on oxidative stress damage induced by H2O2 at 12.5 - 400 µM concentrations was performed with the xCELLigence device to measure cell proliferation in-vitro. In addition, scratch analysis was performed at concentrations of 3.125 - 25 µM in order to determine the wound healing levels in cell damage.
Result and Discussion: The TAC value of oxyresveratrol at 0.5 mg/ml was 3 ± 0.3 and the TOC value was 0.77 ± 0.52; (OSI) value was found to be 0.02 ± 0.09. It has been observed that the total phenolic substance (TPS) concentrations of oxiresveratrol at different doses from 0.625 mg/ml to 10 mg/ml have higher TPS from low concentration to high concentration. Oxyresveratrol L929 fibroblast cells IC50 value 214.2 μM; The IC50 value of oxyresveratrol was determined to be 109.7 μM in the cell line of L929 fibroblast cells exposed to H2O2. The highest cell proliferation was observed when measuring the 12.5 μM concentration of oxiresveratrol with the xCELLigence device. Scratch analysis showed a greater improvement than other doses, with a cell proliferation of 62% at the 24th hour and 88% at the 48th hour at a concentration of 12.5 μM oxyresveratrol in H2O2-damaged cells. The 12.5 μM concentration was determined to be the most effective concentration in both proliferation and scratch analysis. Oxyresveratrol; with its antioxidant capacity at low concentrations, L929 protects fibroblast cells from oxidative stress in cellular damage caused by H2O2, and creates a power multiplier effect on fibroblast viability and migration. These results indicate that oxyresveratrol; showed that it can be effective at the cellular level preventing of acute or chronic diseases caused by free oxygen radicals. It can be thought that oxyresveratrol may be a potential molecule in the treatment of oxidative stress-induced diseases with more comprehensive studies to be carried out at the cellular level and in-vivo studies.

Kaynakça

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  • 18. Hu, S., Chen, F., Wang, M. (2015). Photoprotective effects of oxyresveratrol and Kuwanon O on DNA damage induced by UVA in human epidermal keratinocytes. Chemical Research in Toxicology, 28(3), 541-548. [CrossRef]
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  • 22. Chuanasa, T., Phromjai, J., Lipipun, V., Likhitwitayawuid, K., Suzuki, M., Pramyothin P., Hattori, M., Shiraki, K. (2008). Anti-herpes simplex virus activity of oxyresveratrol derived from Thai medicinal plant: mechanism of action and therapeutic efficacy on cutaneous infection in mice. Antiviral Research, 80, 62-70. [CrossRef]
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  • 24. Andrabi, S.A., Spina, M.G., Lorenz, P., Ebmeyer, U., Wolf, G., Horn, T.F.W. (2004). Oxyresveratrol (trans -2,3,4,5-tetrahydroxystilbene) is neuroprotective and inhibits the apoptotic cell death in transient cerebral ischemia. Brain Research, 1017, 98-107. [CrossRef]
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  • 28. Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103-1111. [CrossRef]
  • 29. Celal, B., Sevindik, M., Akgül, H., Selamoglu, Z. (2019). Oxidative stress index and antioxidant capacity of Lepista nuda collected from Gaziantep/Turkey. Sigma, 37(1), 1-5.
  • 30. Büyüktuncel, E. (2013). Toplam fenolik içerik ve antioksidan kapasite tayininde kullanılan başlıca spektrofotometrik yöntemler. Marmara Pharmaceutical Journal, 17(2), 93-103.
  • 31. Slinkard, K., Singleton, V.L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55. [CrossRef]
  • 32. Twilley, D., Lall, N. (2018). The role of natural products from plants in the development of anticancer agents. In: Mandal, S.C., Mandal. V., Konishi, T. (Eds.), Natural Products and Drug Discovery, (pp. 139-178). Elsevier. [CrossRef]
  • 33. Sırmatel, F., Duygu, F., Çelik, H., Selek, Ş., Sırmatel, Ö., Gürsoy, B., Eriş, F.N. (2009). Kronik Viral Hepatit Olgularında Total Oksidatif Seviye ve Total Antioksidan Kapasitenin Değerlendirilmesi. Klinik Journal/Klimik Dergisi, 22(3), 92-96.
  • 34. Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P.,Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660. [CrossRef]
  • 35. Oliveira, A.M.F., Pinheiro, L.S., Pereira, C.K.S., Matias, W.N., Gomes, R.A., Chaves, O.S., Assis, T.S. (2012). Total phenolic content and antioxidant activity of some Malvaceae family species. Antioxidants, 1(1), 33-43. [CrossRef]
  • 36. Muchuweti, M., Nyamukonda, L., Chagonda, L.S., Ndhlala, A.R., Mupure, C., Benhura, M. (2006). Total phenolic content and antioxidant activity in selected medicinal plants of Zimbabwe. International Journal of Food Science & Technology, 41, 33-38. [CrossRef]
  • 37. Kaur, S., Mondal, P. (2014). Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Journal of Microbiology & Expermientation, 1(1), 23-28. [CrossRef]
  • 38. Sevindik, M., Akgul, H., Pehlivan, M., Selamoglu, Z. (2017). Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresenius Environmental Bulletin, 26(7), 4757-4763.
  • 39. Pehlivan, M., Sevindik, M. (2018). Antioxidant and antimicrobial activities of Salvia multicaulis. Turkish Journal of Agriculture-Food Science and Technology, 6(5), 628-631. [CrossRef]
  • 40. Mohammed, F.S., Akgül, H., Sevindik, M., Khaled, B.M.T. (2018). Phenolic content and biological activities of Rhus coriaria var. zebaria. Fresenius Environmental Bulletin, 27(8), 5694-5702.
  • 41. Mohammed, F.S., Karakaş, M., Akgül, H., Sevindik, M. (2019). Medicinal properties of Allium calocephalum collected from Gara Mountain (Iraq). Fresenius Environmental Bulletin, 28(10), 7419-7426.
  • 42. Mohammed, F.S., Pehlivan, M., Sevindik, M. (2019). Antioxidant, antibacterial and antifungal activities of different extracts of Silybum marianum collected from Duhok (Iraq). International Journal of Secondary Metabolite, 6(4), 317-322. [CrossRef]
  • 43. Mohammed, F.S., Şabik, A.E., Sevindik, E., Pehlivan, M., Sevindik, M. (2020). Determination of Antioxidant and Oxidant Potentials of Thymbra spicata Collected from Duhok-Iraq. Turkish Journal of Agriculture-Food Science and Technology, 8(5), 1171-1173. [CrossRef]
  • 44. Chen, Y., Tseng, S.H., Lai, H.S., Chen, W. J. (2004). Resveratrol-induced cellular apoptosis and cell cycle arrest in neuroblastoma cells and antitumor effects on neuroblastoma in mice. Surgery, 136(1), 57-66. [CrossRef]
  • 45. Kara, A. (2012). Doktora Tezi. Resveratrolün Meme Kanseri Hücre Kültüründeki P53 Aracılı Apoptoz Aktivasyonunun Ve Sitotoksisitesinin Polimeraz Zincir Reaksiyonu (PCR), İmmunsitokimya ve MTT Yöntemleriyle Araştırılması. Histoloji ve Embriyoloji Ana Bilim Dalı, Sağlık Bilimleri Enstitüsü, Atatürk Üniversitesi, Erzurum, Türkiye.
  • 46. Arat, E. (2012). Yüksek LisansTezi. Alchemilla L. Cinsine Ait Bazı Türlerden Elde Edilen Ekstrelerin MCF-7 Kanser ve L929 Fibroblast Hücrelerine Etkisi. Biyoloji Anabilim Dalı, Fen Bilimleri Enstitüsü, Kırıkkale Üniversitesi, Kırıkkale, Türkiye.
  • 47. Duman, İ. (2018). Yüksek LisansTezi. Endometriyum Adenokarsinoma HEC1A Hücrelerinde D Vitamininin Proliferasyon, Migrasyon ve İnvazyon Üzerine Etkisi. Tıbbi Farmakoloji Anabilim Dalı. Sağlık Bilimleri Enstitüsü, Mersin Üniversitesi, Mersin, Türkiye.
  • 48. Pereira, L.O.M., Vilegas, W., Tangerina, M.M.P., Arunachalam, K., Balogun, S.O., Orlandi-Mattos, P. E., Oliveira Martins, D.T. (2018). Lafoensia pacari A. St.-Hil.: Wound healing activity and mechanism of action of standardized hydroethanolic leaves extract. Journal of Ethnopharmacology, 219, 337-350. [CrossRef]
Toplam 48 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Fatih Mehmet Ateş 0000-0002-7497-2211

Yasin Bayır 0000-0003-3562-6727

Erken Görünüm Tarihi 17 Mayıs 2023
Yayımlanma Tarihi 20 Mayıs 2023
Gönderilme Tarihi 2 Şubat 2023
Kabul Tarihi 21 Mart 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 47 Sayı: 2

Kaynak Göster

APA Ateş, F. M., & Bayır, Y. (2023). OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ. Journal of Faculty of Pharmacy of Ankara University, 47(2), 543-556. https://doi.org/10.33483/jfpau.1246570
AMA Ateş FM, Bayır Y. OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ. Ankara Ecz. Fak. Derg. Mayıs 2023;47(2):543-556. doi:10.33483/jfpau.1246570
Chicago Ateş, Fatih Mehmet, ve Yasin Bayır. “OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ”. Journal of Faculty of Pharmacy of Ankara University 47, sy. 2 (Mayıs 2023): 543-56. https://doi.org/10.33483/jfpau.1246570.
EndNote Ateş FM, Bayır Y (01 Mayıs 2023) OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ. Journal of Faculty of Pharmacy of Ankara University 47 2 543–556.
IEEE F. M. Ateş ve Y. Bayır, “OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ”, Ankara Ecz. Fak. Derg., c. 47, sy. 2, ss. 543–556, 2023, doi: 10.33483/jfpau.1246570.
ISNAD Ateş, Fatih Mehmet - Bayır, Yasin. “OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ”. Journal of Faculty of Pharmacy of Ankara University 47/2 (Mayıs 2023), 543-556. https://doi.org/10.33483/jfpau.1246570.
JAMA Ateş FM, Bayır Y. OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ. Ankara Ecz. Fak. Derg. 2023;47:543–556.
MLA Ateş, Fatih Mehmet ve Yasin Bayır. “OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ”. Journal of Faculty of Pharmacy of Ankara University, c. 47, sy. 2, 2023, ss. 543-56, doi:10.33483/jfpau.1246570.
Vancouver Ateş FM, Bayır Y. OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ. Ankara Ecz. Fak. Derg. 2023;47(2):543-56.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.