Araştırma Makalesi
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BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ

Yıl 2023, Cilt: 47 Sayı: 2, 637 - 649, 20.05.2023
https://doi.org/10.33483/jfpau.1211616

Öz

Amaç: Bu araştırmada Düzce'de halk ilacı olarak kullanılan yedi bitkinin [Dioscorea communis (L.) Caddick & Wilkin, Mentha longifolia (L.) L. subsp. typhoides (Briq.) Harley, Origanum vulgare L., Rubus ulmifolius Schott, Salvia tomentosa Mill., Thymus longicaulis C.Presl subsp. longicaulis, Trachystemon orientalis (L.) D. Don] üreaz, kimotripsin inhibe edici ve antioksidan aktivitelerinin tespiti amaçlanmıştır.
Gereç ve Yöntem: Bu amaçla bitkilerden metanol ve su ekstreleri hazırlanmıştır. Daha sonra in vitro üreaz ve kimotripsin inhibitör aktiviteleri belirlenmiştir. Ayrıca farklı yöntemlerle antioksidan aktiviteleri (ABTS, CUPRAC, DPPH), toplam fenol ve flavonoit içerikleri de tespit edilmiştir.
Sonuç ve Tartışma: D. communis, O. vulgare, S. tomentosa ve T. longicaulis'in metanol ekstreleri orta düzeyde üreaz inhibitör aktivite (%34.26 ile %44.94 aralığında inhibisyon) gösterirken, diğerlerinde aktivite daha düşük bulunmuştur. En güçlü kimotripsin inhibe edici aktivite R. ulmifolius metanol ekstresi ve T. orientalis su ekstresinde gözlenmiştir (IC50 değerleri sırasıyla 65.32 ve 78.65µg/ml). Genel olarak, çalışılan bitkiler yüksek fenol ve flavonoit içeriklerine uygun olarak yüksek antioksidan aktivite göstermiştir. Sonuç olarak R. ulmifolius ve T. orientalis kuvvetli kimotripsin inhibe edici aktiviteleri ile dikkat çekmiştir. Bu bitkilerin kimotripsin aktivitesi üzerinde daha kapsamlı çalışmaların yapılması gerekmektedir.

Destekleyen Kurum

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu

Proje Numarası

TBAG-108T253

Teşekkür

Bu çalışma kısmen finansal olarak TÜBİTAK (TBAG-108T253) tarafından desteklenmiştir.

Kaynakça

  • 1. Amtul, Z., Kausar, N., Follmer, C., Rozmahel, R.F., Atta-Ur-Rahman, Kazmi, S.A., Shekhani, M.S., Eriksen, J.L., Khan, K.M., Choudhary, M.I. (2006). Cysteine based novel noncompetitive inhibitors of urease(s)-Distinctive inhibition susceptibility of microbial and plant ureases. Bioorganic & Medicinal Chemistry, 14, 6737-6744. [CrossRef]
  • 2. Mobley, H.L.T., Hausinger, R.P. (1989). Microbial ureases: Significance, regulation, and molecular characterization. Microbiological Reviews, 53(1), 85-108. [CrossRef]
  • 3. Matczuk, D., Siczek, A. (2021). Effectiveness of the use of urease inhibitors in agriculture: a review. International Agrophysics, 35, 197-208. [CrossRef]
  • 4. Modolo, L.V., Souza, A.X.D., Horta, L.P., Araujo, D.P., Fatima, A.D. (2015). An overview on the potential of natural products as ureases inhibitors: A review. Journal of Advanced Research, 6, 35-44. [CrossRef]
  • 5. Legerská, B., Chmelová, D., Ondrejovič, M. (2020). TLC-Bioautography as a fast and cheap screening method for the detection of α-chymotrypsin inhibitors in crude plant extracts. Journal of Biotechnology, 313, 11-17. [CrossRef]
  • 6. Gitlin-Domagalska, A., Maciejewska, A., Debowski, D. (2020). Bowman-Birk inhibitors: Insights into family of multifunctional proteins and peptides with potential therapeutical applications. Pharmaceuticals, 13, 421. [CrossRef]
  • 7. Al-Awadhi, F.H., Law, B.K., Paul, V.J., Luesch, H. (2017). Grassystatins D–F, Potent aspartic protease inhibitors from marine Cyanobacteria as potential antimetastatic agents targeting invasive breast cancer. Journal of Natural Products, 80(11), 2969-2986. [CrossRef]
  • 8. Awosika, T., Aluko, R.E. (2019). Enzymatic pea protein hydrolysates are active trypsin and chymotrypsin inhibitors. Foods, 8(6), 200. [CrossRef]
  • 9. Shi, X.Z., Zhao, X.F., Wang, J.X. (2008). Molecular cloning and expression analysis of chymotrypsin-like serine protease from the Chinese shrimp, Fenneropenaeus chinensis. Fish & Shellfish Immunology, 25, 589-597. [CrossRef]
  • 10. Cid‑Gallegos, M.S., Corzo‑Ríos, L.J., Jiménez‑Martínez, C., Sánchez‑Chino, X.M. (2022). Protease inhibitors from plants as therapeutic agents‑ A Review. Plant Foods for Human Nutrition, 77, 20-29. [CrossRef]
  • 11. Kårlund, A., Paukkonen, I., Gómez-Gallego, C., Kolehmainen, M. (2021). Intestinal exposure to food-derived protease inhibitors: digestion physiology- and gut health-related effects. Healthcare, 9, 1002. [CrossRef]
  • 12. Gürbüz, İ., Özkan, A.M.G., Akaydin, G., Salihoğlu, E., Günbatan, T., Demirci, F., Yeşilada, E. (2019). Folk medicine in Düzce province (Turkey). Turkish Journal of Botany, 43(6), 769-784. [CrossRef]
  • 13. Khan, K.M., Iqbal, S., Lodhi, M.A., Maharvi, G.M., Perveen, S., Choudhary, M.L., Atta-Ur-Rahman, Chohan, Z.H., Supuran, C.T. (2004). Synthesis and urease enzyme inhibitory effects of some dicoumarols. Journal of Enzyme Inhibition and Medicinal Chemistry, 19(4), 367-371. [CrossRef]
  • 14. Cannell, R.J., Kellam, S.J., Owsianka, A.M., Walker, J.M. (1988). Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Medica, 54, 10-14. [CrossRef]
  • 15. Matthaus, B. (2002). Antioxidant activity of extracts obtained from residues of different oilseeds. Journal of Agricultural and Food Chemistry, 50, 3444-3452. [CrossRef]
  • 16. Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E. (2004). A novel total antioxidant capacity index for dietary polyphenols, vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981. [CrossRef]
  • 17. Gülçin, İ. (2007). Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids, 32(3), 431-438. [CrossRef]
  • 18. Woisky, R.G., Salatino, A. (1998). Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37, 99-105. [CrossRef]
  • 19. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158. [CrossRef]
  • 20. Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178. [CrossRef]
  • 21. Farahnaz, N., Eftekhar, F., Habibi, Z., Massarrat, S., Malekzadeh, R. (2009). Antibacterial activity of twenty Iranian plant extracts against clinical isolates of Helicobacter pylori. Iranian Journal of Basic Medical Sciences, 12(2), 105-111. [CrossRef]
  • 22. Bakr, R.O., Tawfike, A., El-Gizawy, H.A., Tawfik, N., Abdelmohsen, U.R., Abdelwahab, M.F., Alshareef, W.A., Fayez, S.M., El-Mancy, S.M.S., El-Fishawy, A.M., Abdelkawyi, M.A., Fayed, M.A.A. (2021). The metabolomic analysis of five Mentha species: Cytotoxicity, anti-Helicobacter assessment, and the development of polymeric micelles for enhancing the anti-Helicobacter activity. RSC Advances, 11, 7318-7330. [CrossRef]
  • 23. Gul, H., Abbas, K., Qadir, M.I. (2015). Gastro-protective effect of ethanolic extract of Mentha longifolia in alcohol- and aspirin-induced gastric ulcer models. Bangladesh Journal of Pharmacology, 10, 241-245. [CrossRef]
  • 24. Özen, T., Telci, İ., Gül, F., Demirtaş, İ. (2018). A comprehensive study on phytochemical contents, isolation and antioxidant capacities in wild mind, Mentha longifolia subsp. typhoides var. typhoides. Moroccan Journal of Chemistry, 6(4), 601-614. [CrossRef]
  • 25. Bahadori, M.B., Zengin, G., Bahadori, S., Dinparast, L., Movahhedin, N. (2018). Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties, 21(1), 183-193. [CrossRef]
  • 26. Elansary, H.O., Szopa, A., Kubica, P., Ekiert, H., Klimek-Szczykutowicz, M., El-Ansary, D.O., Mahmoud, E.A. (2020). Polyphenol profile and antimicrobial and cytotoxic activities of natural mentha piperita and Mentha longifolia populations in Northern Saudi Arabia. Processes, 8(479). [CrossRef]
  • 27. Martini, S., D’Addario, C., Colacevich, A., Focardi, S., Borghini, F., Santucci, A., Figura, N., Rossi, C. (2009). Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds. International Journal of Antimicrobial Agents, 34, 50-59. [CrossRef]
  • 28. Gudej, J., Tomczyk, M. (2004). Determination of flavonoids, tannins and ellagic acid in leaves from Rubus L. Species. Archives of Pharmacal Research, 27(11), 1114-1119.
  • 29. Talukdar, S., Ghosh, K. (2019) Differential inhibition of digestive proteases by tannin in two size groups of rohu (Labeo rohita, Hamilton): A biochemical and zymography study. Aquaculture Research, 50, 449-456. [CrossRef]
  • 30. Liu, J., Hsu, F., Tsai, J., Chan, P., Liu, J., Thomas, G.N., Tomlinson, B., Lo, M., Lin, J. (2003). Antihypertensive effects of tannins isolated from traditional Chinese herbs as non-specific inhibitors of angiontensin converting enzyme. Life Sciences, 73, 1543-1555. [CrossRef]
  • 31. Slavova, I., Tomova, T., Kusovska, S., Chukova, Y., Argirova, M. (2022). Phytochemical constituents and pharmacological potential of Tamus communis rhizomes. Molecules 27, 1851. [CrossRef]
  • 32. Ismail, M.,1 Hussain J., Khan A., Khan A. L., Ali L., Khan F., Khan A.Z., Niaz U., Lee I. (2012). Antibacterial, antifungal, cytotoxic, phytotoxic, insecticidal, and enzyme inhibitory activities of Geranium wallichianum. Evidence -Based Complementary and Alternative Medicine 2012, 305906. [CrossRef]
  • 33. Belkhiri, F., Baghiani, A., Boumerfeg, S., Charef, N., Khennouf, S., Arrar, L. (2015). In vitro antioxidant and antibacterial activities of Tamus communis L. root extracts from Algeria. International Journal of Current Research, 7(05), 15621-15627.
  • 34. Amraoui, N., Mayouf, N., Charef, N., Baghiani, A., Arrar, L. (2019). Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots. Tropical Journal of Pharmaceutical Research, 18(7), 1499-1506. [CrossRef]
  • 35. Dinçer, C., Tontul, İ., Çam, İ.B., Özdemir, K.S., Topuz, A., Nadeem, H.Ş., Ay, S.T., Göktürk, R.S. (2013). Phenolic composition and antioxidant activity of Salvia tomentosa Miller: effects of cultivation, harvesting year, and storage. Turkish Journal of Agriculture and Forestry, 37, 561-567. [CrossRef]
  • 36. Demir, E., Turfan, N., Özer, H., Üstün, N.Ş., Pekşen, A. (2020). Nutrient and bioactive substance contents of edible plants grown naturally in Salıpazarı (Samsun). Acta Scientiarum Polonorum Hortorum Cultus, 19(1), 151-160. [CrossRef]
  • 37. Özen, T. (2010). Antioxidant activity of wild edible plants in the Black Sea Region of Turkey. Grasas y Aceites, 61(1), 86-94. [CrossRef]
  • 38. Kindl, M., Blazekovic, B., Bucar, F., Vladimir-Knezevic, S. (2015). Antioxidant and anticholinesterase potential of six Thymus species. Evidence-Based Complementary and Alternative Medicine, 2015, 403950. [CrossRef]
  • 39. Öztürk, N. (2015). Phenolic composition and antioxidant activity of thedifferent extracts from Thymus longicaulis C Presl. subsp. longicaulis var. longicaulis and T. longicaulis C. Presl. subsp. longicaulis var. subisophyllus growing in Turkey. Pakistan Journal of Pharmaceutical Sciences, 28(2), 465-472.
  • 40. Hanganu, D., Benedec, D., Olah, N.K., Ranga, F., Mırel, S., Tiperciuc, B., Oniga, I. (2020). Research on enzyme inhibition potential and phenolic compounds from Origanum vulgare ssp. vulgare. Farmacia, 68(6), 1075-1080. [CrossRef]
  • 41. Stamatis, G., Kyriazopoulos, P., Golegou, S., Basayiannis, A., Skaltsas, S., Skaltsa, H. (2003). In vitro anti-Helicobacter pylori activity of Greek herbal medicines. Journal of Ethnopharmacology, 88, 175-179. [CrossRef]
  • 42. Chun, S.S., Vattem, D.A., Lin, Y.T., Shetty, K. (2005). Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry, 40, 809-816. [CrossRef]

CHYMOTRYPSIN, UREASE INHIBITORY AND ANTIOXIDANT ACTIVITIES OF SOME DÜZCE FOLK MEDICINES

Yıl 2023, Cilt: 47 Sayı: 2, 637 - 649, 20.05.2023
https://doi.org/10.33483/jfpau.1211616

Öz

Objective: In this study, determining the urease, chymotrypsin inhibitory and antioxidant activities of seven plants [Dioscorea communis (L.) Caddick & Wilkin, Mentha longifolia (L.) L. subsp. typhoides (Briq.) Harley, Origanum vulgare L., Rubus ulmifolius Schott, Salvia tomentosa Mill., Thymus longicaulis C.Presl subsp. longicaulis, Trachystemon orientalis (L.) D. Don] used as folk medicine in Düzce was aimed.
Material and Method: For this purpose, methanol and water extracts were prepared, then in vitro urease and chymotrypsin inhibitory activities were determined. Antioxidant activities (ABTS, CUPRAC, DPPH), total phenol and flavonoid contents were also determined.
Result and Discussion: While D. communis, O. vulgare, S. tomentosa and T. longicaulis methanol extracts showed moderate urease inhibitory activity (inhibitions between 34.26% and 44.94%), the activity was lower in others. The strongest chymotrypsin inhibitory activities were observed in R. ulmifolius methanol extract and T. orientalis water extract (IC50: 65.32 and 78.65 µg/ml, respectively). In general, the studied plants showed high antioxidant activity in accordance with their high phenol and flavonoid contents. In conclusion, R. ulmifolius and T. orientalis attracted attention with strong chymotrypsin inhibitory activities. More comprehensive studies on the chymotrypsin activity of these plants are required.

Proje Numarası

TBAG-108T253

Kaynakça

  • 1. Amtul, Z., Kausar, N., Follmer, C., Rozmahel, R.F., Atta-Ur-Rahman, Kazmi, S.A., Shekhani, M.S., Eriksen, J.L., Khan, K.M., Choudhary, M.I. (2006). Cysteine based novel noncompetitive inhibitors of urease(s)-Distinctive inhibition susceptibility of microbial and plant ureases. Bioorganic & Medicinal Chemistry, 14, 6737-6744. [CrossRef]
  • 2. Mobley, H.L.T., Hausinger, R.P. (1989). Microbial ureases: Significance, regulation, and molecular characterization. Microbiological Reviews, 53(1), 85-108. [CrossRef]
  • 3. Matczuk, D., Siczek, A. (2021). Effectiveness of the use of urease inhibitors in agriculture: a review. International Agrophysics, 35, 197-208. [CrossRef]
  • 4. Modolo, L.V., Souza, A.X.D., Horta, L.P., Araujo, D.P., Fatima, A.D. (2015). An overview on the potential of natural products as ureases inhibitors: A review. Journal of Advanced Research, 6, 35-44. [CrossRef]
  • 5. Legerská, B., Chmelová, D., Ondrejovič, M. (2020). TLC-Bioautography as a fast and cheap screening method for the detection of α-chymotrypsin inhibitors in crude plant extracts. Journal of Biotechnology, 313, 11-17. [CrossRef]
  • 6. Gitlin-Domagalska, A., Maciejewska, A., Debowski, D. (2020). Bowman-Birk inhibitors: Insights into family of multifunctional proteins and peptides with potential therapeutical applications. Pharmaceuticals, 13, 421. [CrossRef]
  • 7. Al-Awadhi, F.H., Law, B.K., Paul, V.J., Luesch, H. (2017). Grassystatins D–F, Potent aspartic protease inhibitors from marine Cyanobacteria as potential antimetastatic agents targeting invasive breast cancer. Journal of Natural Products, 80(11), 2969-2986. [CrossRef]
  • 8. Awosika, T., Aluko, R.E. (2019). Enzymatic pea protein hydrolysates are active trypsin and chymotrypsin inhibitors. Foods, 8(6), 200. [CrossRef]
  • 9. Shi, X.Z., Zhao, X.F., Wang, J.X. (2008). Molecular cloning and expression analysis of chymotrypsin-like serine protease from the Chinese shrimp, Fenneropenaeus chinensis. Fish & Shellfish Immunology, 25, 589-597. [CrossRef]
  • 10. Cid‑Gallegos, M.S., Corzo‑Ríos, L.J., Jiménez‑Martínez, C., Sánchez‑Chino, X.M. (2022). Protease inhibitors from plants as therapeutic agents‑ A Review. Plant Foods for Human Nutrition, 77, 20-29. [CrossRef]
  • 11. Kårlund, A., Paukkonen, I., Gómez-Gallego, C., Kolehmainen, M. (2021). Intestinal exposure to food-derived protease inhibitors: digestion physiology- and gut health-related effects. Healthcare, 9, 1002. [CrossRef]
  • 12. Gürbüz, İ., Özkan, A.M.G., Akaydin, G., Salihoğlu, E., Günbatan, T., Demirci, F., Yeşilada, E. (2019). Folk medicine in Düzce province (Turkey). Turkish Journal of Botany, 43(6), 769-784. [CrossRef]
  • 13. Khan, K.M., Iqbal, S., Lodhi, M.A., Maharvi, G.M., Perveen, S., Choudhary, M.L., Atta-Ur-Rahman, Chohan, Z.H., Supuran, C.T. (2004). Synthesis and urease enzyme inhibitory effects of some dicoumarols. Journal of Enzyme Inhibition and Medicinal Chemistry, 19(4), 367-371. [CrossRef]
  • 14. Cannell, R.J., Kellam, S.J., Owsianka, A.M., Walker, J.M. (1988). Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Medica, 54, 10-14. [CrossRef]
  • 15. Matthaus, B. (2002). Antioxidant activity of extracts obtained from residues of different oilseeds. Journal of Agricultural and Food Chemistry, 50, 3444-3452. [CrossRef]
  • 16. Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E. (2004). A novel total antioxidant capacity index for dietary polyphenols, vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981. [CrossRef]
  • 17. Gülçin, İ. (2007). Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids, 32(3), 431-438. [CrossRef]
  • 18. Woisky, R.G., Salatino, A. (1998). Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37, 99-105. [CrossRef]
  • 19. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158. [CrossRef]
  • 20. Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178. [CrossRef]
  • 21. Farahnaz, N., Eftekhar, F., Habibi, Z., Massarrat, S., Malekzadeh, R. (2009). Antibacterial activity of twenty Iranian plant extracts against clinical isolates of Helicobacter pylori. Iranian Journal of Basic Medical Sciences, 12(2), 105-111. [CrossRef]
  • 22. Bakr, R.O., Tawfike, A., El-Gizawy, H.A., Tawfik, N., Abdelmohsen, U.R., Abdelwahab, M.F., Alshareef, W.A., Fayez, S.M., El-Mancy, S.M.S., El-Fishawy, A.M., Abdelkawyi, M.A., Fayed, M.A.A. (2021). The metabolomic analysis of five Mentha species: Cytotoxicity, anti-Helicobacter assessment, and the development of polymeric micelles for enhancing the anti-Helicobacter activity. RSC Advances, 11, 7318-7330. [CrossRef]
  • 23. Gul, H., Abbas, K., Qadir, M.I. (2015). Gastro-protective effect of ethanolic extract of Mentha longifolia in alcohol- and aspirin-induced gastric ulcer models. Bangladesh Journal of Pharmacology, 10, 241-245. [CrossRef]
  • 24. Özen, T., Telci, İ., Gül, F., Demirtaş, İ. (2018). A comprehensive study on phytochemical contents, isolation and antioxidant capacities in wild mind, Mentha longifolia subsp. typhoides var. typhoides. Moroccan Journal of Chemistry, 6(4), 601-614. [CrossRef]
  • 25. Bahadori, M.B., Zengin, G., Bahadori, S., Dinparast, L., Movahhedin, N. (2018). Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties, 21(1), 183-193. [CrossRef]
  • 26. Elansary, H.O., Szopa, A., Kubica, P., Ekiert, H., Klimek-Szczykutowicz, M., El-Ansary, D.O., Mahmoud, E.A. (2020). Polyphenol profile and antimicrobial and cytotoxic activities of natural mentha piperita and Mentha longifolia populations in Northern Saudi Arabia. Processes, 8(479). [CrossRef]
  • 27. Martini, S., D’Addario, C., Colacevich, A., Focardi, S., Borghini, F., Santucci, A., Figura, N., Rossi, C. (2009). Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds. International Journal of Antimicrobial Agents, 34, 50-59. [CrossRef]
  • 28. Gudej, J., Tomczyk, M. (2004). Determination of flavonoids, tannins and ellagic acid in leaves from Rubus L. Species. Archives of Pharmacal Research, 27(11), 1114-1119.
  • 29. Talukdar, S., Ghosh, K. (2019) Differential inhibition of digestive proteases by tannin in two size groups of rohu (Labeo rohita, Hamilton): A biochemical and zymography study. Aquaculture Research, 50, 449-456. [CrossRef]
  • 30. Liu, J., Hsu, F., Tsai, J., Chan, P., Liu, J., Thomas, G.N., Tomlinson, B., Lo, M., Lin, J. (2003). Antihypertensive effects of tannins isolated from traditional Chinese herbs as non-specific inhibitors of angiontensin converting enzyme. Life Sciences, 73, 1543-1555. [CrossRef]
  • 31. Slavova, I., Tomova, T., Kusovska, S., Chukova, Y., Argirova, M. (2022). Phytochemical constituents and pharmacological potential of Tamus communis rhizomes. Molecules 27, 1851. [CrossRef]
  • 32. Ismail, M.,1 Hussain J., Khan A., Khan A. L., Ali L., Khan F., Khan A.Z., Niaz U., Lee I. (2012). Antibacterial, antifungal, cytotoxic, phytotoxic, insecticidal, and enzyme inhibitory activities of Geranium wallichianum. Evidence -Based Complementary and Alternative Medicine 2012, 305906. [CrossRef]
  • 33. Belkhiri, F., Baghiani, A., Boumerfeg, S., Charef, N., Khennouf, S., Arrar, L. (2015). In vitro antioxidant and antibacterial activities of Tamus communis L. root extracts from Algeria. International Journal of Current Research, 7(05), 15621-15627.
  • 34. Amraoui, N., Mayouf, N., Charef, N., Baghiani, A., Arrar, L. (2019). Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots. Tropical Journal of Pharmaceutical Research, 18(7), 1499-1506. [CrossRef]
  • 35. Dinçer, C., Tontul, İ., Çam, İ.B., Özdemir, K.S., Topuz, A., Nadeem, H.Ş., Ay, S.T., Göktürk, R.S. (2013). Phenolic composition and antioxidant activity of Salvia tomentosa Miller: effects of cultivation, harvesting year, and storage. Turkish Journal of Agriculture and Forestry, 37, 561-567. [CrossRef]
  • 36. Demir, E., Turfan, N., Özer, H., Üstün, N.Ş., Pekşen, A. (2020). Nutrient and bioactive substance contents of edible plants grown naturally in Salıpazarı (Samsun). Acta Scientiarum Polonorum Hortorum Cultus, 19(1), 151-160. [CrossRef]
  • 37. Özen, T. (2010). Antioxidant activity of wild edible plants in the Black Sea Region of Turkey. Grasas y Aceites, 61(1), 86-94. [CrossRef]
  • 38. Kindl, M., Blazekovic, B., Bucar, F., Vladimir-Knezevic, S. (2015). Antioxidant and anticholinesterase potential of six Thymus species. Evidence-Based Complementary and Alternative Medicine, 2015, 403950. [CrossRef]
  • 39. Öztürk, N. (2015). Phenolic composition and antioxidant activity of thedifferent extracts from Thymus longicaulis C Presl. subsp. longicaulis var. longicaulis and T. longicaulis C. Presl. subsp. longicaulis var. subisophyllus growing in Turkey. Pakistan Journal of Pharmaceutical Sciences, 28(2), 465-472.
  • 40. Hanganu, D., Benedec, D., Olah, N.K., Ranga, F., Mırel, S., Tiperciuc, B., Oniga, I. (2020). Research on enzyme inhibition potential and phenolic compounds from Origanum vulgare ssp. vulgare. Farmacia, 68(6), 1075-1080. [CrossRef]
  • 41. Stamatis, G., Kyriazopoulos, P., Golegou, S., Basayiannis, A., Skaltsas, S., Skaltsa, H. (2003). In vitro anti-Helicobacter pylori activity of Greek herbal medicines. Journal of Ethnopharmacology, 88, 175-179. [CrossRef]
  • 42. Chun, S.S., Vattem, D.A., Lin, Y.T., Shetty, K. (2005). Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry, 40, 809-816. [CrossRef]
Toplam 42 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Tuğba Günbatan 0000-0002-1138-3145

Ece Miser Salihoğlu 0000-0003-0681-3566

İlhan Gürbüz 0000-0002-3670-0899

Sevgi Akaydın 0000-0002-0927-5188

Galip Akaydın 0000-0003-3728-0176

Proje Numarası TBAG-108T253
Erken Görünüm Tarihi 28 Nisan 2023
Yayımlanma Tarihi 20 Mayıs 2023
Gönderilme Tarihi 2 Aralık 2022
Kabul Tarihi 18 Nisan 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 47 Sayı: 2

Kaynak Göster

APA Günbatan, T., Miser Salihoğlu, E., Gürbüz, İ., Akaydın, S., vd. (2023). BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ. Journal of Faculty of Pharmacy of Ankara University, 47(2), 637-649. https://doi.org/10.33483/jfpau.1211616
AMA Günbatan T, Miser Salihoğlu E, Gürbüz İ, Akaydın S, Akaydın G. BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ. Ankara Ecz. Fak. Derg. Mayıs 2023;47(2):637-649. doi:10.33483/jfpau.1211616
Chicago Günbatan, Tuğba, Ece Miser Salihoğlu, İlhan Gürbüz, Sevgi Akaydın, ve Galip Akaydın. “BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ”. Journal of Faculty of Pharmacy of Ankara University 47, sy. 2 (Mayıs 2023): 637-49. https://doi.org/10.33483/jfpau.1211616.
EndNote Günbatan T, Miser Salihoğlu E, Gürbüz İ, Akaydın S, Akaydın G (01 Mayıs 2023) BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ. Journal of Faculty of Pharmacy of Ankara University 47 2 637–649.
IEEE T. Günbatan, E. Miser Salihoğlu, İ. Gürbüz, S. Akaydın, ve G. Akaydın, “BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ”, Ankara Ecz. Fak. Derg., c. 47, sy. 2, ss. 637–649, 2023, doi: 10.33483/jfpau.1211616.
ISNAD Günbatan, Tuğba vd. “BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ”. Journal of Faculty of Pharmacy of Ankara University 47/2 (Mayıs 2023), 637-649. https://doi.org/10.33483/jfpau.1211616.
JAMA Günbatan T, Miser Salihoğlu E, Gürbüz İ, Akaydın S, Akaydın G. BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ. Ankara Ecz. Fak. Derg. 2023;47:637–649.
MLA Günbatan, Tuğba vd. “BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ”. Journal of Faculty of Pharmacy of Ankara University, c. 47, sy. 2, 2023, ss. 637-49, doi:10.33483/jfpau.1211616.
Vancouver Günbatan T, Miser Salihoğlu E, Gürbüz İ, Akaydın S, Akaydın G. BAZI DÜZCE HALK İLAÇLARININ KİMOTRİPSİN, ÜREAZ İNHİBE EDİCİ VE ANTİOKSİDAN AKTİVİTELERİ. Ankara Ecz. Fak. Derg. 2023;47(2):637-49.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.