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CYTOTOXIC AND ANTIMICROBIAL ACTIVITIES OF PTILOSTEMON CHAMAEPEUCE (L.) LESS.

Yıl 2022, Cilt: 46 Sayı: 3, 694 - 702, 30.09.2022

Serdar Demir Yalçın Erzurumlu İsmail Öztürk Petek Ballar Kırmızıbayrak Canan Karaalp

https://doi.org/10.33483/jfpau.1100161

Öz

Objective: The aim of this study is to investigate the antimicrobial and cytotoxic activities of n-hexane chloroform and methanol extracts prepared from the aerial parts of P. chamaepeuce (L.) Less. (Asteraceae).
Material and Method: The antimicrobial activities of the extracts on standard bacterial strains (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Bacillus subtilis and Streptococcus pneumoniae) and fungal strains (Candida albicans and C. parapsilosis) were examined by microdilution method and minimum inhibitory concentration (MIC) values were determined. The cytotoxic activity of the extracts was investigated by cell proliferation assay using WST-1 reagent on various cancer cell lines including HeLa, U2OS, PC3, MCF-7, and A549 cancer cell lines.
Result and Discussion: The MIC values of n-hexane, chloroform and methanol extracts were between 250-1000 µg/mL against S. aureus, E. faecalis and B. subtilis. n-hexane and chloroform extracts had varying levels of cytotoxic activity in all tested cancer cell lines (IC50: 21.0-67.1 μg/mL).

Anahtar Kelimeler

Antimicrobial activity cytotoxicity Ptilostemon chamaepeuce

Kaynakça

  • 1. Ekim, T. (2012). Ptilostemon Cass. In: Guner, A., Aslan, S., Ekim, T., Vural, M., Babac, M.T., (Eds.), Türkiye Bitkileri Listesi (Damarlı Bitkiler), (pp. 193). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği.
  • 2. Vilatersana, R., Garcia-Jacas, N., Garnatje, T., Molero, J., Sonnante, G., Susanna, A. (2010). Molecular phylogeny of the genus Ptilostemon (Compositae: Cardueae) and its relationships with Cynara and Lamyropsis. Systematic Botany, 35(4), 907-917. [CrossRef]
  • 3. Badalamenti, N., Modica, A., Ilardi, V., Bruno, M. (2021). The chemical composition of the essential oil of Ptilostemon gnaphaloides subsp. pseudofruticosus (Asteraceae) growing in Kythira Island, Greece. Natural Product Research, 1-5. [CrossRef]
  • 4. Gonzalez-Tejero, M.R., Casares-Porcel, M., Sanchez-Rojas, C.P., Ramiro-Gutierrez, J.M., Molero-Mesa, J., Pieroni, A., Giusti, M.E., Censorii, E., de Pasquale, C., Della, A., Paraskeva-Hadijchambi, D., Hadjichambis, A., Houmani, Z., El-Demerdash, M., El-Zayat, M., Hmamouchi, M., ElJohrig, S. (2008). Medicinal plants in the Mediterranean area: Synthesis of the results of the project Rubia. Journal of Ethnopharmacology, 116(2), 341-357. [CrossRef]
  • 5. Bohlmann, F., Ziesche, J. (1980). Neue guajanolide und acetylenverbindungen aus Ptilostemon-arten. Phytochemistry, 19(4), 692-696. [CrossRef]
  • 6. Menichini, F., Di Benedetto, R., Delle Monache, F. (1996). A triterpene epoxide and a guaianolide from Ptilostemmon gnaphaloides. Phytochemistry, 41(5), 1377-1379. [CrossRef]
  • 7. Di Stefano, V., Pitonzo, R. (2012). Phytochemical studies on Ptilostemon greuteri Raimondo & Domina (Compositae). Records of Natural Products, 6(4), 390-393. [CrossRef]
  • 8. Menichini, F., Di Benedetto, R., Delle Monache, G., Delle Monache, E. (1986). Sesquiterpenes from Ptilostemon niveus. Fitoterapia, 57, 458-459.
  • 9. Janackovic, P., Tesevic, V., Marin, P.D., Milosavljevic, S.M., Petkovic, B., Sokovic, M. (2002). Polyacetylenes and a sesquiterpene lactone from Ptilostemon strictus. Biochemical Systematics and Ecology, 30(1), 69-71. [CrossRef]
  • 10. Marengo, A., Maxia, A., Sanna, C., Mandrone, M., Bertea, C.M., Bicchi, C., Sgorbini, B., Cagliero, C., Rubiolo, P. (2019). Intra-specific variation in the little-known Mediterranean plant Ptilostemon casabonae (L.) Greuter analysed through phytochemical and biomolecular markers. Phytochemistry, 161, 21-27. [CrossRef]
  • 11. Djordjevic, I., Tesevic, V., Milosavljevic, S., Menkovic, N., Vajs, V., Jadranin, M., Jovanovic, A., Djokovic, D. (2008). Composition of the essential oil of Ptilostemon gnaphaloides. Chemistry of Natural Compounds, 44(5), 668-669. [CrossRef]
  • 12. Kurt, A., Ozcan, M., Colak, N., Ozogul, Y., Glew, R., Ozogul, F., Ayaz, F.A. (2019). Fatty acids of oil and antioxidant capacity of phenolics from fruits of 11 Cardueae (Carduoideae, Asteraceae) taxa from northeast Anatolia (Turkey). Botanica Serbica, 43(1), 31-45. [CrossRef]
  • 13. Marengo, A., Fumagalli, M., Sanna, C., Maxia, A., Piazza, S., Cagliero, C., Rubiolo, P., Sangiovanni, E., Dell'Agli, M. (2018). The hydro-alcoholic extracts of Sardinian wild thistles (Onopordum spp.) inhibit TNF alpha-induced IL-8 secretion and NF-kappa B pathway in human gastric epithelial AGS cells. Journal of Ethnopharmacology, 210, 469-476. [CrossRef]
  • 14. Turker, A.U., Yildirim, A.B. (2013). Evaluation of antibacterial and antitumor activities of some Turkish endemic plants. Tropical Journal of Pharmaceutical Research, 12(6), 1003-1010. [CrossRef]
  • 15. Calderon-Montano, J.M., Martinez-Sanchez, S.M., Burgos-Moron, E., Guillen-Mancina, E., Jimenez-Alonso, J.J., Garcia, F., Aparicio, A., Lopez-Lazaro, M. (2019). Screening for selective anticancer activity of plants from Grazalema Natural Park, Spain. Natural Product Research, 33(23), 3454-3458. [CrossRef]
  • 16. Bohlmann, F., Rao, N., Schwarz, H. (1974). Naturally occuring terpene derivatives XXIX. Two new sesquiterpenes from Ptilostemon chamaepeuce (L.) Less. Chemische Berichte, 107, 650-653. [CrossRef]
  • 17. Bruno, M., Maggio, A., Paternostro, M.P., Rosselli, S., Arnold, N.A., Herz, W. (2001). Sesquiterpene lactones and other constituents of three Cardueae from Cyprus. Biochemical Systematics and Ecology, 29(4), 433-435. [CrossRef]
  • 18. Fokialakis, N., Kalpoutzakis, E., Tekwani, B.L., Khan, S.I., Kobaisy, M., Skaltsounis, A.L., Duke, S.O. (2007). Evaluation of the antimalarial and antileishmanial activity of plants from the Greek island of Crete. Journal of Natural Medicines, 61, 38-45. [CrossRef]
  • 19. Clinical and Laboratory Standards Institute (CLSI). (2013). Performance Standards for Antimicrobial Susceptibility Testing, 23rd Informational Supplement. CLSI document M100-S23. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, USA.
  • 20. Baykan Erel, S., Senol, S.G., Aydın Köse, F., Ballar, P. (2011). In vitro cytotoxic properties of six Artemisia L. species. Turkish Journal of Pharmaceutical Sciences, 8(3), 247-252. [CrossRef]
  • 21. Thavamani, B.S., Mathew, M., Dhanabal, S.P. (2013). In vitro cytotoxic activity of menispermaceae plants against HeLa cell line. Ancient Science of Life, 33(2), 81-84. [CrossRef]
  • 22. Turker, H., Yildirim, A.B. (2015). Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. Biotechnology & Biotechnological Equipment, 29(2), 281-288. [CrossRef]
  • 23. Abu-Izneid, T., Rauf, A., Shariati, M.A., Khalil, A.A., Imran, M., Rebezov, M., Uddin, M.S., Mahomoodally, M.F., Rengasamy, K.R.R. (2020). Sesquiterpenes and their derivatives-natural anticancer compounds: An update. Pharmacological Research, 161, 105165. [CrossRef]
  • 24. Choi, S.Z., Choi, S.U., Lee, K.R. (2005). Cytotoxic sesquiterpene lactones from Saussurea calcicola. Archives of Pharmacal Research, 28(10), 1142-1146. [CrossRef]

PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ

Yıl 2022, Cilt: 46 Sayı: 3, 694 - 702, 30.09.2022

Serdar Demir Yalçın Erzurumlu İsmail Öztürk Petek Ballar Kırmızıbayrak Canan Karaalp

https://doi.org/10.33483/jfpau.1100161

Öz

Amaç: Bu çalışmanın amacı, P. chamaepeuce (L.) Less. (Asteraceae)’nin topraküstü kısımlarından hazırlanan n-hekzan, kloroform ve metanol ekstrelerinin antimikrobiyal ve sitotoksik aktivitelerinin araştırılmasıdır.
Gereç ve Yöntem: Ekstrelerin antimikrobiyal aktiviteleri, standart bakteri kökenleri (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Bacillus subtilis ve Streptococcus pneumoniae) ve mantar kökenleri (Candida albicans ve C. parapsilosis) üzerinde mikrodilüsyon metodu ile araştırılmış ve minimum inhibitör konsantrasyon (MİK) değerleri belirlenmiştir. Ekstrelerin sitotoksik aktivitesi, WST-1 reaktifi kullanılarak hücre proliferasyon analiz yöntemi ile HeLa, U2OS, PC3, MCF-7 ve A549 olmak üzere farklı kanser hücre hatları üzerinde incelenmiştir.
Sonuç ve Tartışma: Bitkiden elde edilen n-hekzan, kloroform ve metanol ekstrelerinin MİK değerlerinin S. aureus, E. faecalis ve B. subtilis’e karşı 250-1000 µg/mL konsantrasyon aralığında olduğu saptanmıştır. n-hekzan ve kloroform ekstrelerinin tüm kanser hücre hatlarında ise değişen düzeyde sitotoksik aktiviteye sahip oldukları belirlenmiştir (IC50: 21.0-67.1 μg/mL).

Anahtar Kelimeler

Ptilostemon chamaepeuce Antimikrobiyal aktivite Sitotoksisite

Kaynakça

  • 1. Ekim, T. (2012). Ptilostemon Cass. In: Guner, A., Aslan, S., Ekim, T., Vural, M., Babac, M.T., (Eds.), Türkiye Bitkileri Listesi (Damarlı Bitkiler), (pp. 193). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği.
  • 2. Vilatersana, R., Garcia-Jacas, N., Garnatje, T., Molero, J., Sonnante, G., Susanna, A. (2010). Molecular phylogeny of the genus Ptilostemon (Compositae: Cardueae) and its relationships with Cynara and Lamyropsis. Systematic Botany, 35(4), 907-917. [CrossRef]
  • 3. Badalamenti, N., Modica, A., Ilardi, V., Bruno, M. (2021). The chemical composition of the essential oil of Ptilostemon gnaphaloides subsp. pseudofruticosus (Asteraceae) growing in Kythira Island, Greece. Natural Product Research, 1-5. [CrossRef]
  • 4. Gonzalez-Tejero, M.R., Casares-Porcel, M., Sanchez-Rojas, C.P., Ramiro-Gutierrez, J.M., Molero-Mesa, J., Pieroni, A., Giusti, M.E., Censorii, E., de Pasquale, C., Della, A., Paraskeva-Hadijchambi, D., Hadjichambis, A., Houmani, Z., El-Demerdash, M., El-Zayat, M., Hmamouchi, M., ElJohrig, S. (2008). Medicinal plants in the Mediterranean area: Synthesis of the results of the project Rubia. Journal of Ethnopharmacology, 116(2), 341-357. [CrossRef]
  • 5. Bohlmann, F., Ziesche, J. (1980). Neue guajanolide und acetylenverbindungen aus Ptilostemon-arten. Phytochemistry, 19(4), 692-696. [CrossRef]
  • 6. Menichini, F., Di Benedetto, R., Delle Monache, F. (1996). A triterpene epoxide and a guaianolide from Ptilostemmon gnaphaloides. Phytochemistry, 41(5), 1377-1379. [CrossRef]
  • 7. Di Stefano, V., Pitonzo, R. (2012). Phytochemical studies on Ptilostemon greuteri Raimondo & Domina (Compositae). Records of Natural Products, 6(4), 390-393. [CrossRef]
  • 8. Menichini, F., Di Benedetto, R., Delle Monache, G., Delle Monache, E. (1986). Sesquiterpenes from Ptilostemon niveus. Fitoterapia, 57, 458-459.
  • 9. Janackovic, P., Tesevic, V., Marin, P.D., Milosavljevic, S.M., Petkovic, B., Sokovic, M. (2002). Polyacetylenes and a sesquiterpene lactone from Ptilostemon strictus. Biochemical Systematics and Ecology, 30(1), 69-71. [CrossRef]
  • 10. Marengo, A., Maxia, A., Sanna, C., Mandrone, M., Bertea, C.M., Bicchi, C., Sgorbini, B., Cagliero, C., Rubiolo, P. (2019). Intra-specific variation in the little-known Mediterranean plant Ptilostemon casabonae (L.) Greuter analysed through phytochemical and biomolecular markers. Phytochemistry, 161, 21-27. [CrossRef]
  • 11. Djordjevic, I., Tesevic, V., Milosavljevic, S., Menkovic, N., Vajs, V., Jadranin, M., Jovanovic, A., Djokovic, D. (2008). Composition of the essential oil of Ptilostemon gnaphaloides. Chemistry of Natural Compounds, 44(5), 668-669. [CrossRef]
  • 12. Kurt, A., Ozcan, M., Colak, N., Ozogul, Y., Glew, R., Ozogul, F., Ayaz, F.A. (2019). Fatty acids of oil and antioxidant capacity of phenolics from fruits of 11 Cardueae (Carduoideae, Asteraceae) taxa from northeast Anatolia (Turkey). Botanica Serbica, 43(1), 31-45. [CrossRef]
  • 13. Marengo, A., Fumagalli, M., Sanna, C., Maxia, A., Piazza, S., Cagliero, C., Rubiolo, P., Sangiovanni, E., Dell'Agli, M. (2018). The hydro-alcoholic extracts of Sardinian wild thistles (Onopordum spp.) inhibit TNF alpha-induced IL-8 secretion and NF-kappa B pathway in human gastric epithelial AGS cells. Journal of Ethnopharmacology, 210, 469-476. [CrossRef]
  • 14. Turker, A.U., Yildirim, A.B. (2013). Evaluation of antibacterial and antitumor activities of some Turkish endemic plants. Tropical Journal of Pharmaceutical Research, 12(6), 1003-1010. [CrossRef]
  • 15. Calderon-Montano, J.M., Martinez-Sanchez, S.M., Burgos-Moron, E., Guillen-Mancina, E., Jimenez-Alonso, J.J., Garcia, F., Aparicio, A., Lopez-Lazaro, M. (2019). Screening for selective anticancer activity of plants from Grazalema Natural Park, Spain. Natural Product Research, 33(23), 3454-3458. [CrossRef]
  • 16. Bohlmann, F., Rao, N., Schwarz, H. (1974). Naturally occuring terpene derivatives XXIX. Two new sesquiterpenes from Ptilostemon chamaepeuce (L.) Less. Chemische Berichte, 107, 650-653. [CrossRef]
  • 17. Bruno, M., Maggio, A., Paternostro, M.P., Rosselli, S., Arnold, N.A., Herz, W. (2001). Sesquiterpene lactones and other constituents of three Cardueae from Cyprus. Biochemical Systematics and Ecology, 29(4), 433-435. [CrossRef]
  • 18. Fokialakis, N., Kalpoutzakis, E., Tekwani, B.L., Khan, S.I., Kobaisy, M., Skaltsounis, A.L., Duke, S.O. (2007). Evaluation of the antimalarial and antileishmanial activity of plants from the Greek island of Crete. Journal of Natural Medicines, 61, 38-45. [CrossRef]
  • 19. Clinical and Laboratory Standards Institute (CLSI). (2013). Performance Standards for Antimicrobial Susceptibility Testing, 23rd Informational Supplement. CLSI document M100-S23. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, USA.
  • 20. Baykan Erel, S., Senol, S.G., Aydın Köse, F., Ballar, P. (2011). In vitro cytotoxic properties of six Artemisia L. species. Turkish Journal of Pharmaceutical Sciences, 8(3), 247-252. [CrossRef]
  • 21. Thavamani, B.S., Mathew, M., Dhanabal, S.P. (2013). In vitro cytotoxic activity of menispermaceae plants against HeLa cell line. Ancient Science of Life, 33(2), 81-84. [CrossRef]
  • 22. Turker, H., Yildirim, A.B. (2015). Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. Biotechnology & Biotechnological Equipment, 29(2), 281-288. [CrossRef]
  • 23. Abu-Izneid, T., Rauf, A., Shariati, M.A., Khalil, A.A., Imran, M., Rebezov, M., Uddin, M.S., Mahomoodally, M.F., Rengasamy, K.R.R. (2020). Sesquiterpenes and their derivatives-natural anticancer compounds: An update. Pharmacological Research, 161, 105165. [CrossRef]
  • 24. Choi, S.Z., Choi, S.U., Lee, K.R. (2005). Cytotoxic sesquiterpene lactones from Saussurea calcicola. Archives of Pharmacal Research, 28(10), 1142-1146. [CrossRef]
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Serdar Demir 0000-0001-6572-8818

Yalçın Erzurumlu 0000-0001-6835-4436

İsmail Öztürk 0000-0002-2669-3090

Petek Ballar Kırmızıbayrak 0000-0002-6189-1818

Canan Karaalp 0000-0002-4787-3779

Yayımlanma Tarihi 30 Eylül 2022
Gönderilme Tarihi 11 Nisan 2022
Kabul Tarihi 16 Mayıs 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 46 Sayı: 3

Kaynak Göster

APA Demir, S., Erzurumlu, Y., Öztürk, İ., Ballar Kırmızıbayrak, P., vd. (2022). PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ. Journal of Faculty of Pharmacy of Ankara University, 46(3), 694-702. https://doi.org/10.33483/jfpau.1100161
AMA Demir S, Erzurumlu Y, Öztürk İ, Ballar Kırmızıbayrak P, Karaalp C. PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ. Ankara Ecz. Fak. Derg. Eylül 2022;46(3):694-702. doi:10.33483/jfpau.1100161
Chicago Demir, Serdar, Yalçın Erzurumlu, İsmail Öztürk, Petek Ballar Kırmızıbayrak, ve Canan Karaalp. “PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ”. Journal of Faculty of Pharmacy of Ankara University 46, sy. 3 (Eylül 2022): 694-702. https://doi.org/10.33483/jfpau.1100161.
EndNote Demir S, Erzurumlu Y, Öztürk İ, Ballar Kırmızıbayrak P, Karaalp C (01 Eylül 2022) PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ. Journal of Faculty of Pharmacy of Ankara University 46 3 694–702.
IEEE S. Demir, Y. Erzurumlu, İ. Öztürk, P. Ballar Kırmızıbayrak, ve C. Karaalp, “PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ”, Ankara Ecz. Fak. Derg., c. 46, sy. 3, ss. 694–702, 2022, doi: 10.33483/jfpau.1100161.
ISNAD Demir, Serdar vd. “PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ”. Journal of Faculty of Pharmacy of Ankara University 46/3 (Eylül 2022), 694-702. https://doi.org/10.33483/jfpau.1100161.
JAMA Demir S, Erzurumlu Y, Öztürk İ, Ballar Kırmızıbayrak P, Karaalp C. PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ. Ankara Ecz. Fak. Derg. 2022;46:694–702.
MLA Demir, Serdar vd. “PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ”. Journal of Faculty of Pharmacy of Ankara University, c. 46, sy. 3, 2022, ss. 694-02, doi:10.33483/jfpau.1100161.
Vancouver Demir S, Erzurumlu Y, Öztürk İ, Ballar Kırmızıbayrak P, Karaalp C. PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ. Ankara Ecz. Fak. Derg. 2022;46(3):694-702.
 Kapak Resmi İndir

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.