Araştırma Makalesi
BibTex RIS Kaynak Göster

Determination of betalactoglobulin gene polymorphism in Kivircik sheep

Yıl 2018, Cilt: 1 Sayı: 1, 13 - 17, 01.01.2018

Eser Kemal Gürcan Özden Çobanoğlu Elif Almaz

Öz

This study was carried out to determine the genetic polymorphism of the β-lactoglobulin (β-Lg) gene for indigenous Kivircik breed in Turkey. As to find the Kivircik Sheep is limited as purebred, this breed was raised and maintained in special farm with small numbers as pure breeds. Due to animal material was constituted 48 heads as purebred Kivircik sheep. The study was realized by means of PCR-RFLP methods. Also, two genetic alleles (A and B) and three genotypes (AA, AB and BB) were detected for this purebred. The gene frequencies of β-Lg A and B were calculated as 0.469 and 0.531 in Kivircik sheep. The population was found in equilibrium according to Hardy-Weinberg. At the same time, Some F statistics values were calculated like effective alleles number (Ne), observed heterozygosity (Ho), expected heterozygosity (He) and fixation index (Fis); 1.992, 0.396, 0.498 and 0.205, respectively.

Anahtar Kelimeler

Kivircik β-Lg Milk protein Polymorphism PCR-RFLP

Kaynakça

  • Anton I, Zsolnai A, Fesus L, Kukovics S, Molnar A. 1999.Survey of β-lactoglobulin and alfa S1-Casein polymorphism in Hungariandairy sheep breeds and crosses on DNA level. ArchTierzuct, 42: 387-392.
  • Anton I, Zsolnai A, Fesus L. 2005. β-lactoglobulin and alfa S1-casein polymorphism in Hungarian sheep breeds and crosses on DNA level. Vratnadolina, 63-67.
  • Araro R, Bhatia S, Mishra B P, Sharma R, Pandey A K, Prakash B, Jain A. 2010. Genetic polymorphism of the β-lactoglobulin gene in native sheep from India. Biochem Genet, 48 (3-4): 304-311 .
  • Aschaffenburg R, Drewry J. 1957. Genetics of the β-lactoglobulins of cow's Milk. Nature, 180: 376-378.
  • Ashton G C. 1957. Serum protein differences in cattle by Starch-Jel Electrophoresis. Nature, 180: 917-919.
  • Baranyi M, Kerekes A, Hiripi L, Bozse Z. 2010. Preliminary data on beta lactoglobulin genetic polymorphisms in HungaryAwassi and Racka sheep. Romanian Academy, DAGENE-2010.
  • Çelik Ş, Özdemir S. 2006. β-Lactoglobulin variants in Awassi and Morkaraman sheep and theirassociationwith the composition and rennetclotting time of the milk. Turk J Vet Anim Sci, 30: 539-544.
  • DemirciM.1995. Süt Teknolojisine Giriş. Trakya Üniversitesi Tekirdağ Ziraat Fakültesi Yayın No: 105, Ders Notu No: 68, Tekirdağ.
  • Elmacı C, Öner Y, Balcioglu M S. 2006. Genetic polymorphism of β-lactoglobulin gene in nativeTurkish sheep breeds. Biochem Genet, 44 (7-8): 376-381.
  • Elmacı C, Öner Y,Balcioglu M S. 2012. β-lactoglobulin gene types in Karacabey Merino sheep breeds using PCR-RFLP. J Appl Anim Res, 32 (2): 145-148.
  • Erdogan F. 2009. Yerli gen kaynağı Çine Çaparı koyunlarının süt verim özellikleri ve Beta laktoglobulin gen polimorfizmi. M. Sc. Thesis (unpublished). ADÜ, Fen BilimleriEnstitüsü Aydın.
  • Feligini M, Parma P, Aleandri R, Greppi GF, Enne E. 1998. PCR-RFLP test for direct determination of β-lactoglobulin genotype in sheep. Anim Genet, 29 (6): 473-474.
  • Khaertdinov R A. 1990. The effect of genotype on milk proteins in Kholmogor and Ayrshire cows. Anim Breed Abst, 54 (7): 4355.
  • Mclean DM, Graham ERB, Ponzoni RW, Mckenzie HA. 1984. Effect of milk protein genetic variants on milk yield and composition. Anim Breed Abst, 53(10): 6206.
  • Michalova A, Krupuva Z. 2009. Influence of β-lactoglobulin genotypes on composition of milk and milk production traits of the Slovak ovine breeds. Acta Fytotechinica et Zootech Mimoriadne Cislo, 2009.
  • Mroczkowski S,Korman K, Erhardt G, Piwezynski D, Borys B. 2004. Sheep milk protein polymorphism and its effect on milk performance of Polish Merino. Arch Tierz Dummerstorf, 47 Special Issue, 114-121.
  • Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natn. Acad. Sci. U.S.A. 70, 3321-3323.
  • Ng-Kwai- Hang, KF, Hayes J F,Moxley J F,Monardes H G. 1986. Relationships between milk protein polymorphism and majör milk constituents in Holstein Friesian cows. Anim Breed Abst, 53 (10): 6206.
  • Ng-Kwai- Hang, K F, Monardes H G, Hayes J F. 1990. Association between genetic polymorphism of milk protein and production traits during three lactations. Anim Breed Abst, 59 (7): 4614.
  • Rensburg LV, Lange DJD, Gindra A. 1990. The polymorphic protein system in cows milk: II. The phenotype frequencies of whey proteins in different herds of Holstein Friesian and Jersey cows in the republic of South Africa. Anim Breed Abst, 59 (12): 8211.
  • Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed., chapter 6, 22, Cold Spring Harbor Laboratory, Cold-Spring Harbor, New York.
  • Yeh F, Yang RC, Boyle T. 2000. PopGene (v.1.32), Microsoft Windows-based freewarefor population genetic analysis. Retrieved from http://www.ualberta.ca/fyeh/Pop32.exe.

Yıl 2018, Cilt: 1 Sayı: 1, 13 - 17, 01.01.2018

Eser Kemal Gürcan Özden Çobanoğlu Elif Almaz

Öz

Kaynakça

  • Anton I, Zsolnai A, Fesus L, Kukovics S, Molnar A. 1999.Survey of β-lactoglobulin and alfa S1-Casein polymorphism in Hungariandairy sheep breeds and crosses on DNA level. ArchTierzuct, 42: 387-392.
  • Anton I, Zsolnai A, Fesus L. 2005. β-lactoglobulin and alfa S1-casein polymorphism in Hungarian sheep breeds and crosses on DNA level. Vratnadolina, 63-67.
  • Araro R, Bhatia S, Mishra B P, Sharma R, Pandey A K, Prakash B, Jain A. 2010. Genetic polymorphism of the β-lactoglobulin gene in native sheep from India. Biochem Genet, 48 (3-4): 304-311 .
  • Aschaffenburg R, Drewry J. 1957. Genetics of the β-lactoglobulins of cow's Milk. Nature, 180: 376-378.
  • Ashton G C. 1957. Serum protein differences in cattle by Starch-Jel Electrophoresis. Nature, 180: 917-919.
  • Baranyi M, Kerekes A, Hiripi L, Bozse Z. 2010. Preliminary data on beta lactoglobulin genetic polymorphisms in HungaryAwassi and Racka sheep. Romanian Academy, DAGENE-2010.
  • Çelik Ş, Özdemir S. 2006. β-Lactoglobulin variants in Awassi and Morkaraman sheep and theirassociationwith the composition and rennetclotting time of the milk. Turk J Vet Anim Sci, 30: 539-544.
  • DemirciM.1995. Süt Teknolojisine Giriş. Trakya Üniversitesi Tekirdağ Ziraat Fakültesi Yayın No: 105, Ders Notu No: 68, Tekirdağ.
  • Elmacı C, Öner Y, Balcioglu M S. 2006. Genetic polymorphism of β-lactoglobulin gene in nativeTurkish sheep breeds. Biochem Genet, 44 (7-8): 376-381.
  • Elmacı C, Öner Y,Balcioglu M S. 2012. β-lactoglobulin gene types in Karacabey Merino sheep breeds using PCR-RFLP. J Appl Anim Res, 32 (2): 145-148.
  • Erdogan F. 2009. Yerli gen kaynağı Çine Çaparı koyunlarının süt verim özellikleri ve Beta laktoglobulin gen polimorfizmi. M. Sc. Thesis (unpublished). ADÜ, Fen BilimleriEnstitüsü Aydın.
  • Feligini M, Parma P, Aleandri R, Greppi GF, Enne E. 1998. PCR-RFLP test for direct determination of β-lactoglobulin genotype in sheep. Anim Genet, 29 (6): 473-474.
  • Khaertdinov R A. 1990. The effect of genotype on milk proteins in Kholmogor and Ayrshire cows. Anim Breed Abst, 54 (7): 4355.
  • Mclean DM, Graham ERB, Ponzoni RW, Mckenzie HA. 1984. Effect of milk protein genetic variants on milk yield and composition. Anim Breed Abst, 53(10): 6206.
  • Michalova A, Krupuva Z. 2009. Influence of β-lactoglobulin genotypes on composition of milk and milk production traits of the Slovak ovine breeds. Acta Fytotechinica et Zootech Mimoriadne Cislo, 2009.
  • Mroczkowski S,Korman K, Erhardt G, Piwezynski D, Borys B. 2004. Sheep milk protein polymorphism and its effect on milk performance of Polish Merino. Arch Tierz Dummerstorf, 47 Special Issue, 114-121.
  • Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natn. Acad. Sci. U.S.A. 70, 3321-3323.
  • Ng-Kwai- Hang, KF, Hayes J F,Moxley J F,Monardes H G. 1986. Relationships between milk protein polymorphism and majör milk constituents in Holstein Friesian cows. Anim Breed Abst, 53 (10): 6206.
  • Ng-Kwai- Hang, K F, Monardes H G, Hayes J F. 1990. Association between genetic polymorphism of milk protein and production traits during three lactations. Anim Breed Abst, 59 (7): 4614.
  • Rensburg LV, Lange DJD, Gindra A. 1990. The polymorphic protein system in cows milk: II. The phenotype frequencies of whey proteins in different herds of Holstein Friesian and Jersey cows in the republic of South Africa. Anim Breed Abst, 59 (12): 8211.
  • Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed., chapter 6, 22, Cold Spring Harbor Laboratory, Cold-Spring Harbor, New York.
  • Yeh F, Yang RC, Boyle T. 2000. PopGene (v.1.32), Microsoft Windows-based freewarefor population genetic analysis. Retrieved from http://www.ualberta.ca/fyeh/Pop32.exe.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Research Articles
Yazarlar

Eser Kemal Gürcan

Özden Çobanoğlu

Elif Almaz Bu kişi benim

Yayımlanma Tarihi 1 Ocak 2018
Gönderilme Tarihi 18 Aralık 2017
Yayımlandığı Sayı Yıl 2018 Cilt: 1 Sayı: 1

Kaynak Göster

APA Gürcan, E. K., Çobanoğlu, Ö., & Almaz, E. (2018). Determination of betalactoglobulin gene polymorphism in Kivircik sheep. Black Sea Journal of Engineering and Science, 1(1), 13-17.
AMA Gürcan EK, Çobanoğlu Ö, Almaz E. Determination of betalactoglobulin gene polymorphism in Kivircik sheep. BSJ Eng. Sci. Ocak 2018;1(1):13-17.
Chicago Gürcan, Eser Kemal, Özden Çobanoğlu, ve Elif Almaz. “Determination of Betalactoglobulin Gene Polymorphism in Kivircik Sheep”. Black Sea Journal of Engineering and Science 1, sy. 1 (Ocak 2018): 13-17.
EndNote Gürcan EK, Çobanoğlu Ö, Almaz E (01 Ocak 2018) Determination of betalactoglobulin gene polymorphism in Kivircik sheep. Black Sea Journal of Engineering and Science 1 1 13–17.
IEEE E. K. Gürcan, Ö. Çobanoğlu, ve E. Almaz, “Determination of betalactoglobulin gene polymorphism in Kivircik sheep”, BSJ Eng. Sci., c. 1, sy. 1, ss. 13–17, 2018.
ISNAD Gürcan, Eser Kemal vd. “Determination of Betalactoglobulin Gene Polymorphism in Kivircik Sheep”. Black Sea Journal of Engineering and Science 1/1 (Ocak 2018), 13-17.
JAMA Gürcan EK, Çobanoğlu Ö, Almaz E. Determination of betalactoglobulin gene polymorphism in Kivircik sheep. BSJ Eng. Sci. 2018;1:13–17.
MLA Gürcan, Eser Kemal vd. “Determination of Betalactoglobulin Gene Polymorphism in Kivircik Sheep”. Black Sea Journal of Engineering and Science, c. 1, sy. 1, 2018, ss. 13-17.
Vancouver Gürcan EK, Çobanoğlu Ö, Almaz E. Determination of betalactoglobulin gene polymorphism in Kivircik sheep. BSJ Eng. Sci. 2018;1(1):13-7.
 Kapak Resmi İndir

                                                24890