Araştırma Makalesi
MICROSATELLITE AND ALLOZYME VARIATIONS IN STARLET STURGEON WILD BROODSTOCK AND HATCHERY-PRODUCED OFFSPRING, USED FOR RESTOCKING OF LOWER DANUBE RIVER
Öz
The natural
population of all sturgeon species has been seriously affected by
overexploitation in combination with a substantial loss and degradation of
habitats during the 20th century. Fish species undergoing to
restocking require information about the genetic identity of the existing fish
populations. The genetic pattern of sterlet (broodstock and offspring) were
analyzed, using allozymes and seven microsatellite loci (LS19, LS34, LS39,
LS54, LS68, Aox45 and Aox27) as markers for species identification. From the
seven microsatellite loci three (LS57, Aox23 and Aox45) have shown a tetrasomic
profile. The most polymorphic locus for the broodstock was LS-57 with 12
alleles in population, followed by Aox45, LS-68 and LS-54 with 10 alleles. For offspring,
the most polymorphic locus was Aox 23 with 11 alleles. Other loci presented a lower
level of polymorphism range between 2 to 8 alleles identified for the analyzed
individuals. The average observed and expected heterozygosities were 0.429 and
0.413 in the broodstock and 0.500 and 0.423 in the offspring, respectively. The
preliminary data showed that selected allozymes and microsatellite markers
allow identification of the stellate sturgeon from Bulgarian farms and could be applied to test and control the broodstocks used for restocking programmes.
Anahtar Kelimeler
Kaynakça
- Burcea, A., Florescu, I., Dudu, A., Georgescu, S., & Costache, M. (2014). Molecular methods for the detection of natural hybrids in sturgeon populations. Transylvanian Review of Systematical and Ecological Research, 16(1), 65-72.
- Dobrovolov I. (1973). Microelectrophoresis on starch gel. Proceeding of Research Institute of Fisheries, 12, 157-162.
- Dobrovolov, I. (1976). Multiple forms of lactate dehydrogenase in anchovy (Engraulis encrasicholus L.) from the Black Sea, Sea of Azov and the Atlantic Ocean. Comptes rendus de l'Académie bulgare des Sciences, 29, 877-880.
- Dobrovolov, I., Tsekov, A., Ivanova, P., & Yankova, M. (2004). Identification on the black cavier of great sturgeon Huso huso and sterlet Acipenser ruthenus (Acipenseridae, Pisces). Journal of Animal Science, XLI(3), 30-32.
- Dobrovolov, I., Ivanova, P., & Tzekov, A. (2005). Genetic-biochemical identification of some sturgeons and their hybrids (Pisces, Acipenseridae). Verhandlungen des Internationalen Verein Limnologie, 29(2), 917-921.
- Dudu, A., Suciu, R., Paraschiv, M., Georgescu, S.E., Costach,e M., & Berrebi, P. (2011). Nuclear markers of Danube sturgeons hybridization. International journal of molecular Sciences, 12, 6796-6809.
- Dudu, A., Georgescu, S., Burcea, A., Florescu, I., & Costache, M. (2013). Microsatellites variation in sterlet sturgeon, Acipenser rhutenus from the Lower Danube. Animal sciences and Biotechnologies, 46(1), 90-94.
- Dudu, A., Macarie, R.D., Burcea, A., & Georgescu, S.E., & Costache M. (2015). Identification of bester hybrid and its parental species (♀ Huso huso Linnaeus, 1758 and ♂ Acipenser ruthenus Linnaeus, 1758) by nuclear markers. Animal Science and Biotechnologies, 48(1), 81-85.
- Havelka, M., Kašpar, V., Hulák, M., & Flajšhans, M. (2011). Sturgeon genetics and cytogenetics: a review related to ploidy levels and interspecific hybridization. Folia Zooligica, 60(2), 93-103.
- Fopp-Bayat, D., Kuciński, M., Liszewski, T., Teodorowicz, T., Łączyńska, B., & Lebeda I. (2015). Genetic protocol of atlantic sturgeon Acipenser oxyrinchus (L.) fry for restocking the Vistula river, Poland. Journal of Survey in Fisheries Sciences, 2(1), 1-10.
- Peakall, R. & Smouse, P.E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28, 2537-2539.
- Raymakers, C. (2007). CITES, the Convention on International Trade in Endangered Species of Wild Fauna and Flora: its role in the conservation of Acipenseriformes. Journal of Applied Ichthyology, 22, 53–65.
- Rosenthal, H., Pourkazemi, M., & Bruch R. (2006). The 5th international Symposium on sturgeons. A conference with major emphasis on conservation, environmental mitigation and sustainable use of the sturgeon resources. Journal of Applied Ichthyology, 22, 1-4 .
- Shaw, C.H.R. & Prasad R. (1970). Starch gel electrophoresis of enzymes – a comparison of recipes. Biochemical Genetics, 4, 297-320.
- Shaklee, J.B., Allendorf, F.W., Morizot, D.C., & Whit, G.S. (1990). Gene nomenclature for protein-coding loci in Fish, Transactions of American Fisheries Society, 119, 2-15.
- Tsekov, А., Ivanova, P., Angelov, M., Atanasova, S., Bloesch, J. (2008). Natural sturgeon hybrids along the Bulgarian Black Sea coast and in the Danube River, Acta Zoologica Bulgarica, 60(3), 311-316.
- Yudha, T.A., Jan-Lung, C., & Jin- Chywan G. (2012). Genetic diversity of Indonesian milkfish (Chanos chanos) using Amplified Fragment Length Polymorphism (AFLP) analysis. African Journal of Biotechnology, 11(13), 3055 -3060.
Yıl 2017,
Cilt: 3 Sayı: 4, 199 - 206, 30.09.2017
Öz
Kaynakça
- Burcea, A., Florescu, I., Dudu, A., Georgescu, S., & Costache, M. (2014). Molecular methods for the detection of natural hybrids in sturgeon populations. Transylvanian Review of Systematical and Ecological Research, 16(1), 65-72.
- Dobrovolov I. (1973). Microelectrophoresis on starch gel. Proceeding of Research Institute of Fisheries, 12, 157-162.
- Dobrovolov, I. (1976). Multiple forms of lactate dehydrogenase in anchovy (Engraulis encrasicholus L.) from the Black Sea, Sea of Azov and the Atlantic Ocean. Comptes rendus de l'Académie bulgare des Sciences, 29, 877-880.
- Dobrovolov, I., Tsekov, A., Ivanova, P., & Yankova, M. (2004). Identification on the black cavier of great sturgeon Huso huso and sterlet Acipenser ruthenus (Acipenseridae, Pisces). Journal of Animal Science, XLI(3), 30-32.
- Dobrovolov, I., Ivanova, P., & Tzekov, A. (2005). Genetic-biochemical identification of some sturgeons and their hybrids (Pisces, Acipenseridae). Verhandlungen des Internationalen Verein Limnologie, 29(2), 917-921.
- Dudu, A., Suciu, R., Paraschiv, M., Georgescu, S.E., Costach,e M., & Berrebi, P. (2011). Nuclear markers of Danube sturgeons hybridization. International journal of molecular Sciences, 12, 6796-6809.
- Dudu, A., Georgescu, S., Burcea, A., Florescu, I., & Costache, M. (2013). Microsatellites variation in sterlet sturgeon, Acipenser rhutenus from the Lower Danube. Animal sciences and Biotechnologies, 46(1), 90-94.
- Dudu, A., Macarie, R.D., Burcea, A., & Georgescu, S.E., & Costache M. (2015). Identification of bester hybrid and its parental species (♀ Huso huso Linnaeus, 1758 and ♂ Acipenser ruthenus Linnaeus, 1758) by nuclear markers. Animal Science and Biotechnologies, 48(1), 81-85.
- Havelka, M., Kašpar, V., Hulák, M., & Flajšhans, M. (2011). Sturgeon genetics and cytogenetics: a review related to ploidy levels and interspecific hybridization. Folia Zooligica, 60(2), 93-103.
- Fopp-Bayat, D., Kuciński, M., Liszewski, T., Teodorowicz, T., Łączyńska, B., & Lebeda I. (2015). Genetic protocol of atlantic sturgeon Acipenser oxyrinchus (L.) fry for restocking the Vistula river, Poland. Journal of Survey in Fisheries Sciences, 2(1), 1-10.
- Peakall, R. & Smouse, P.E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28, 2537-2539.
- Raymakers, C. (2007). CITES, the Convention on International Trade in Endangered Species of Wild Fauna and Flora: its role in the conservation of Acipenseriformes. Journal of Applied Ichthyology, 22, 53–65.
- Rosenthal, H., Pourkazemi, M., & Bruch R. (2006). The 5th international Symposium on sturgeons. A conference with major emphasis on conservation, environmental mitigation and sustainable use of the sturgeon resources. Journal of Applied Ichthyology, 22, 1-4 .
- Shaw, C.H.R. & Prasad R. (1970). Starch gel electrophoresis of enzymes – a comparison of recipes. Biochemical Genetics, 4, 297-320.
- Shaklee, J.B., Allendorf, F.W., Morizot, D.C., & Whit, G.S. (1990). Gene nomenclature for protein-coding loci in Fish, Transactions of American Fisheries Society, 119, 2-15.
- Tsekov, А., Ivanova, P., Angelov, M., Atanasova, S., Bloesch, J. (2008). Natural sturgeon hybrids along the Bulgarian Black Sea coast and in the Danube River, Acta Zoologica Bulgarica, 60(3), 311-316.
- Yudha, T.A., Jan-Lung, C., & Jin- Chywan G. (2012). Genetic diversity of Indonesian milkfish (Chanos chanos) using Amplified Fragment Length Polymorphism (AFLP) analysis. African Journal of Biotechnology, 11(13), 3055 -3060.
Toplam 17 adet kaynakça vardır.
Ayrıntılar
| Konular | Hidrobiyoloji |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Eylül 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 3 Sayı: 4 |
