Year 2019, Volume 5, Issue 1, Pages 34 - 40 2019-04-25

Dietary Protein Requirements of Zebrafish (Dania rerio)
Zebra Balığının (Dania rerio) Diyetsel Protein Gereksinimi

Hüseyin SEVGİLİ [1] , Soner SEZEN [2] , Mahir KANYILMAZ [3] , Özgür AKTAŞ [4] , Faruk PAK [5]

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Zebrafish (Danio rerio) with an initial weight of 88.61±0.82 mg were fed eight isoenergetic diets containing dietary protein levels ranging from 20 to 55 % by 5 % increments. Each diet was feed in triplicate of fish for 6 weeks. Specific growth rates (SGR) at week 2 and 4 were quadratically affected by the treatments but this trend disappeared at the end of the experiment. Dietary protein levels linearly reduced the values of daily feed intake, feed conversion ratio and protein efficiency rate. The whole body dry matter, ash and lipid concentrations linearly decreased with dietary protein levels whereas whole body protein was quadratically affected. The second order polynomial and two break point linear models (TBPLM) were used to estimate dietary protein requirements. The later model generated lower residual sum of squares when SGRWeek4 and SGRFinal valueswere used as a response. Minimum dietary protein requirements for SGRWeek4 and SGRFinal were estimated by the TBPLM as 27.69 and 28.93 % respectively. Briefly, results of the study suggest a minimum dietary protein requirement of zebrafish is about 29 % for maximum growth rate.

Ortalama başlangıç ağırlığı 88,61±0,82 mg olan zebra balıkları (Danio rerio) protein düzeyi % 20-55 arasında değişen sekiz adet izoenerjitik yemle beslenmiştir. Her bir deneme yemi üç tekrarlı olarak 6 hafta boyunca balıklara verilmiştir. Spesifik büyüme oranı (SGR) 2. ve 4. haftalarda kuadratik olarak etkilenirken, bu eğilim deneme sonunda kaybolmuştur. Protein düzeyleri arttıkça yem tüketimi, yemden yararlanma oranı ve protein etkinlik oranı doğrusal olarak düşmüştür. Tüm vücut kuru madde, kül ve lipit konsantrasyonları diyetsel protein düzeyinin artışı ile doğrusal olarak düşmüş, vücut protein düzeyi ise kuadratik olarak etkilenmiştir. Diyetsel protein gereksinimlerini tahmin etmek için, ikinci derece regresyon ve iki kırıklı linear model (İKLM) kullanılmıştır. 4. hafta ve deneme sonu SGR değerleri kullanıldığında İKLM daha düşük kalıntı kareler toplamı vermiştir. 4. hafta ve final SGR oranlarına göre, İKLM minimum protein gereksinimlerini sırasıyla, % 27,69 ve % 28,93 olarak tahmin etmiştir. Kısaca, çalışma bulguları zebra balıklarının maksimum büyüme için minimum protein gereksinimlerinin yaklaşık % 29 olduğunu göstermektedir.


  • Akpιnar Z, Sevgili H, Özgen T, Demir A, Emre Y. 2012. Dietary protein requirement of juvenile shi drum, Umbrina cirrosa (L.). Aquacult Res. 43:421-429. doi: 10.1111/j.1365-2109.2011.02845.x
  • AOAC. 1990. Official Methods of Analysis, 15 ed., Association of Official Analytical Chemists, Arlington, VA, p.684
  • Biga PR, Goetz FW. 2006. Zebrafish and giant danio as models for muscle growth: determinate vs. indeterminate growth as determined by morphometric analysis. Am J Physiol Regul Integr Comp Physiol. 291:R1327-R1337. doi: 10.1152/ajpregu.00905.2005
  • Eaton RC, Farley RD. 1974. Growth and the reduction of depensation of zebrafish, Brachydanio rerio, reared in the laboratory. Copeia. 1974(1):204-209. doi: 10.2307/1443024
  • El‐Dakar AY, Shalaby SM, Saoud IP. 2011. Dietary protein requirement of juvenile marbled spinefoot rabbitfish Siganus rivulatus. Aquacult Res. 42:1050-1055. doi: 10.1111/j.1365-2109.2010.02694.x
  • Fernandes H, Peres H, Carvalho AP. 2016. Dietary protein requirement during juvenile growth of Zebrafish (Danio rerio). Zebrafish. 13(6):548-555. doi: 10.1089/zeb.2016.1303
  • Gurure R, Moccia R, Atkinson J. 1995. Optimal protein requirements of young Arctic charr (Salvelinus alpinus) fed practical diets. Aquacult Nutr. 1:227-234. doi: 10.1111/j.1365-2095.1995.tb00048.x
  • Hernandez-Llamas A. 2009. Conventional and alternative dose–response models to estimate nutrient requirements of aquaculture species. Aquaculture. 292:207-213. doi: 10.1016/j.aquaculture.2009.04.014
  • Kim S-S, Lee K-J. 2009. Dietary protein requirement of juvenile tiger puffer (Takifugu rubripes). Aquaculture. 287(1-2):219-222. doi: 10.1016/j.aquaculture.2008.10.021
  • Klatt SF, von Danwitz A, Hasler M, Susenbeth A. 2016. Determination of the lower and upper critical concentration of Methionine+ Cystine in diets of juvenile turbot (Psetta maxima). Aquaculture. 452:12-23. doi: 10.1016/j.aquaculture.2015.10.015
  • Lawrence C. 2007. The husbandry of zebrafish (Danio rerio): a review. Aquaculture. 269(1-4):1-20. doi: 10.1016/j.aquaculture.2007.04.077
  • Markovich ML, Rizzuto NV, Brown PB. 2007. Diet affects spawning in zebrafish. Zebrafish. 4(1):69-74. doi: 10.1089/zeb.2006.9993
  • NRC. 2011. Nutrient requirements of fish and shrimp. National Research Council of the National Academies, Washington DC.
  • O'Brine TM, Vrtělová J, Snellgrove DL, Davies SJ, Sloman KA. 2015. Growth, oxygen consumption, and behavioral responses of Danio rerio to variation in dietary protein and lipid levels. Zebrafish 12(4):296-304. doi: 10.1089/zeb.2014.1008
  • Robison BD, Drewa RE, Murdoch GK, Powell M, Rodnick KJ, Settles M, Stone D, Churchill A, Hill RA, Papasani MR, Lewis SS, Hardy RW. 2008. Sexual dimorphism in hepatic gene expression and the response to dietary carbohydrate manipulation in the zebrafish (Danio rerio). Comp Biochem Physiol Part D Genomics Proteomics. 3(2):141-154. doi: 10.1016/j.cbd.2008.01.001
  • Shearer K. 2000. Experimental design, statistical analysis and modelling of dietary nutrient requirement studies for fish: a critical review. Aquacult Nutr. 6(2):91-102. doi: 10.1046/j.1365-2095.2000.00134.x
  • Shyong W-J, Huang C-H, Chen H-C. 1998. Effects of dietary protein concentration on growth and muscle composition of juvenile Zacco barbata. Aquaculture. 167:35-42. doi: 10.1016/S0044-8486(98)00313-5
  • Siccardi III AJ, Garris HW, Jones WT, Moseley DB, D'Abramo LR, Watts SA. 2009. Growth and survival of zebrafish (Danio rerio) fed different commercial and laboratory diets. Zebrafish. 6(3):275-280. doi: 10.1089/zeb.2008.0553
  • Smith Jr DL, Barry RJ, Powell ML, Nagy TR, D'Abramo L, Watts SA. 2013. Dietary protein source influence on body size and composition in growing zebrafish. Zebrafish. 10(3):439-446. doi: 10.1089/zeb.2012.0864
  • Ulloa PE, Iturra P, Neira R, Araneda C. 2011. Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes. Rev Fish Biol Fisheries 21(4):649-666. doi: 10.1007/s11160-011-9203-0
  • Ulloa PE, Medrano JF, Feijoo CG. 2014. Zebrafish as animal model for aquaculture nutrition research. Front Genet. 5:313. doi: 10.3389/fgene.2014.00313
  • Yossa R, Sarker PK, Mock DM, Vandenberg GW. 2014. Dietary biotin requirement for growth of juvenile zebrafish Danio rerio (Hamilton-Buchanan). Aquacult Res. 45(11):1787-1797. doi: 10.1111/are.12124
Primary Language en
Subjects Fisheries
Published Date April 2019
Journal Section Research Article
Authors

Orcid: 0000-0001-8274-7391
Author: Hüseyin SEVGİLİ (Primary Author)
Institution: Mediterranean Fisheries Research Production and Training Institute, Kepez Unit, 07192, Döşemealtı, Antalya
Country: Turkey


Orcid: 0000-0002-7055-1284
Author: Soner SEZEN

Orcid: 0000-0003-0261-1422
Author: Mahir KANYILMAZ
Institution: Mediterranean Fisheries Research Production and Training Institute, Kepez Unit, 07192, Döşemealtı, Antalya
Country: Turkey


Orcid: 0000-0002-3175-2251
Author: Özgür AKTAŞ

Orcid: 0000-0003-4191-6173
Author: Faruk PAK

Bibtex @research article { limnofish440537, journal = {Journal of Limnology and Freshwater Fisheries Research}, issn = {}, eissn = {2149-4428}, address = {Fisheries Research Institute}, year = {2019}, volume = {5}, pages = {34 - 40}, doi = {10.17216/limnofish.440537}, title = {Dietary Protein Requirements of Zebrafish (Dania rerio)}, key = {cite}, author = {SEVGİLİ, Hüseyin and SEZEN, Soner and KANYILMAZ, Mahir and AKTAŞ, Özgür and PAK, Faruk} }
APA SEVGİLİ, H , SEZEN, S , KANYILMAZ, M , AKTAŞ, Ö , PAK, F . (2019). Dietary Protein Requirements of Zebrafish (Dania rerio). Journal of Limnology and Freshwater Fisheries Research, 5 (1), 34-40. DOI: 10.17216/limnofish.440537
MLA SEVGİLİ, H , SEZEN, S , KANYILMAZ, M , AKTAŞ, Ö , PAK, F . "Dietary Protein Requirements of Zebrafish (Dania rerio)". Journal of Limnology and Freshwater Fisheries Research 5 (2019): 34-40 <http://www.limnofish.org/issue/44372/440537>
Chicago SEVGİLİ, H , SEZEN, S , KANYILMAZ, M , AKTAŞ, Ö , PAK, F . "Dietary Protein Requirements of Zebrafish (Dania rerio)". Journal of Limnology and Freshwater Fisheries Research 5 (2019): 34-40
RIS TY - JOUR T1 - Dietary Protein Requirements of Zebrafish (Dania rerio) AU - Hüseyin SEVGİLİ , Soner SEZEN , Mahir KANYILMAZ , Özgür AKTAŞ , Faruk PAK Y1 - 2019 PY - 2019 N1 - doi: 10.17216/limnofish.440537 DO - 10.17216/limnofish.440537 T2 - Journal of Limnology and Freshwater Fisheries Research JF - Journal JO - JOR SP - 34 EP - 40 VL - 5 IS - 1 SN - -2149-4428 M3 - doi: 10.17216/limnofish.440537 UR - https://doi.org/10.17216/limnofish.440537 Y2 - 2018 ER -
EndNote %0 Journal of Limnology and Freshwater Fisheries Research Dietary Protein Requirements of Zebrafish (Dania rerio) %A Hüseyin SEVGİLİ , Soner SEZEN , Mahir KANYILMAZ , Özgür AKTAŞ , Faruk PAK %T Dietary Protein Requirements of Zebrafish (Dania rerio) %D 2019 %J Journal of Limnology and Freshwater Fisheries Research %P -2149-4428 %V 5 %N 1 %R doi: 10.17216/limnofish.440537 %U 10.17216/limnofish.440537
ISNAD SEVGİLİ, Hüseyin , SEZEN, Soner , KANYILMAZ, Mahir , AKTAŞ, Özgür , PAK, Faruk . "Dietary Protein Requirements of Zebrafish (Dania rerio)". Journal of Limnology and Freshwater Fisheries Research 5 / 1 (April 2019): 34-40. https://doi.org/10.17216/limnofish.440537