Cilt 3, Sayı 1, Sayfalar 25 - 31 2017-04-24

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Yusuf BOZKURT [1] , İlker YAVAŞ [2]

230 256

Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straws volumes and thawing rates on the post-thaw quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4°C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour   (for 10 min at -120ºC) and finally stored in liquid nitrogen (-196ºC) tank. Thawing of cryopreserved semen process was performed at 30ºC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using ratio of 1x105 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30ºC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

Cyprinus carpio,cryopreservation,spermatozoa motility,straw volume,thawing rate
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Konular Yaşam Bilimleri
Yayımlanma Tarihi 2017
Dergi Bölümü Araştırma Makalesi
Yazarlar

Orcid: orcid.org/0000-0002-6162-2466
Yazar: Yusuf BOZKURT
E-posta: yfbozkurt@hotmail.com
Kurum: Iskenderun Technical University, Faculty of Marine Sciences and Technology
Ülke: Turkey


Yazar: İlker YAVAŞ
E-posta: iyavas09@hotmail.com
Kurum: MUSTAFA KEMAL UNIV
Ülke: Turkey


Bibtex @araştırma makalesi { limnofish277835, journal = {Journal of Limnology and Freshwater Fisheries Research}, issn = {}, address = {Su Ürünleri Araştırma Enstitüsü}, year = {2017}, volume = {3}, pages = {25 - 31}, doi = {10.17216/limnofish.277835}, title = {Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm}, language = {en}, key = {cite}, author = {YAVAŞ, İlker and BOZKURT, Yusuf} }
APA BOZKURT, Y , YAVAŞ, İ . (2017). Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm. Journal of Limnology and Freshwater Fisheries Research, 3 (1), 25-31. DOI: 10.17216/limnofish.277835
MLA BOZKURT, Y , YAVAŞ, İ . "Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm". Journal of Limnology and Freshwater Fisheries Research 3 (2017): 25-31 <http://www.limnofish.org/issue/28724/277835>
Chicago BOZKURT, Y , YAVAŞ, İ . "Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm". Journal of Limnology and Freshwater Fisheries Research 3 (2017): 25-31
RIS TY - JOUR T1 - Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm AU - Yusuf BOZKURT , İlker YAVAŞ Y1 - 2017 PY - 2017 N1 - doi: 10.17216/limnofish.277835 DO - 10.17216/limnofish.277835 T2 - Journal of Limnology and Freshwater Fisheries Research JF - Journal JO - JOR SP - 25 EP - 31 VL - 3 IS - 1 SN - -2149-4428 M3 - doi: 10.17216/limnofish.277835 UR - http://dx.doi.org/10.17216/limnofish.277835 Y2 - 2017 ER -
EndNote %0 Journal of Limnology and Freshwater Fisheries Research Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm %A Yusuf BOZKURT , İlker YAVAŞ %T Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm %D 2017 %J Journal of Limnology and Freshwater Fisheries Research %P -2149-4428 %V 3 %N 1 %R doi: 10.17216/limnofish.277835 %U 10.17216/limnofish.277835