Yıl 2015, Cilt 1, Sayı 2, Sayfalar 75 - 81 2015-08-28

Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts
Karasu Nehri (Erzurum) Havzasında Yaşayan Pelophylax ridibundus (Anura: Ranidae) Kurbağalarında Kirliliğin Neden Olduğu Eritrositik Çekirdek Anormalliklerinin Tespiti

Turgay ŞİŞMAN [1] , Hakan AŞKIN [2] , Hasan TÜRKEZ [3] , Hakan ÖZKAN [4] , Ümit İNCEKARA [5] , Suat ÇOLAK [6]

339 804

Karasu River, which is the only river in the Erzurum plain, is the source of the river Euphrates (Eastern Anatolia of Turkey). The river is in a serious environmental situation as a result of pollution by agricultural and industrial sewage and domestic discharges. The present study aims to evaluate genotoxic effects of toxic metals in marsh frog, Pelophylax ridibundus collected from two contaminated wetlands environments of the Karasu River Basin, in comparison with frogs from one non-polluted reference site. Heavy metal concentrations in surface water of the river were determined. Genotoxicity assays such as micronucleus (MN) and other nuclear abnormalities (NA) were carried out on the frogs studied. MN and NA (kidney-shaped nucleus, notched nucleus and lobed nucleus) were assessed in peripheral blood erythrocytes of the frog. A significant elevation in MN and NA frequencies was observed in frog collected from the polluted sites compared with those from the reference site. Results of the current study suggest that there is a close realtion between increasing of erythrocytic nuclear abnormalities and environmental contamination. High concentrations of heavy metals have a potential genotoxic effects, and the toxicity is possibly related to industrial, agricultural and domestic activities.

Erzurum ovasının tek nehri olan Karasu Nehri, Fırat Nehri’nin kaynağını oluşturmaktadır. Nehirde tarımsal, endüstriyel ve evsel atıkların karışması sonucu ciddi bir çevresel kirlilik durumu söz konusudur. Bu çalışmanın amacı nehrin metallerle kirlenmiş ve kirlenmemiş bölgelerinde yaşayan ova kurbağalarındaki (Pelophylax ridibundus) genotoksik etkileri araştırmaktır. Kirliliği tespit etmek için nehrin daha yüzey suyu örneklerinde ağır metallerin konsantrasyonları ölçülmüştür. Kurbağalarda genotoksisite mikro çekirdek (MÇ) ve diğer çekirdek anormalliklerinin (ÇA) oluşumuna bakılarak tespit edilmiştir. MÇ ve böbrek şekilli çekirdek, çentikli çekirdek ve loplu çekirdek gibi diğer ÇA oluşumları kurbağaların eritrositleri kullanılarak belirlenmiştir. Kirlenmiş bölge kurbağalarının kanlarında MÇ ve ÇA frekansı temiz bölge kurbağalarına göre önemli ölçüde arttığı bulunmuştur. Bu artış ile nehrin kirlenmiş olma durumu arasında kuvvetli ilişkinin olduğu sonucuna varılmıştır. Yüksek ağır metal seviyesinin toksisiteye yol açtığı ve tespit edilen genotoksisitenin endüstriyel, tarımsal ve evsel aktivitelerle ilgili olabileceği kanaatine varılmıştır.

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Birincil Dil en
Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Turgay ŞİŞMAN

Yazar: Hakan AŞKIN

Yazar: Hasan TÜRKEZ

Yazar: Hakan ÖZKAN

Yazar: Ümit İNCEKARA

Yazar: Suat ÇOLAK

Bibtex @ { limnofish205741, journal = {Journal of Limnology and Freshwater Fisheries Research}, issn = {}, eissn = {2149-4428}, address = {Su Ürünleri Araştırma Enstitüsü}, year = {2015}, volume = {1}, pages = {75 - 81}, doi = {10.17216/LimnoFish-5000115825}, title = {Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts}, key = {cite}, author = {İNCEKARA, Ümit and ŞİŞMAN, Turgay and ÇOLAK, Suat and TÜRKEZ, Hasan and ÖZKAN, Hakan and AŞKIN, Hakan} }
APA ŞİŞMAN, T , AŞKIN, H , TÜRKEZ, H , ÖZKAN, H , İNCEKARA, Ü , ÇOLAK, S . (2015). Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts. Journal of Limnology and Freshwater Fisheries Research, 1 (2), 75-81. DOI: 10.17216/LimnoFish-5000115825
MLA ŞİŞMAN, T , AŞKIN, H , TÜRKEZ, H , ÖZKAN, H , İNCEKARA, Ü , ÇOLAK, S . "Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts". Journal of Limnology and Freshwater Fisheries Research 1 (2015): 75-81 <http://www.limnofish.org/issue/19384/205741>
Chicago ŞİŞMAN, T , AŞKIN, H , TÜRKEZ, H , ÖZKAN, H , İNCEKARA, Ü , ÇOLAK, S . "Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts". Journal of Limnology and Freshwater Fisheries Research 1 (2015): 75-81
RIS TY - JOUR T1 - Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts AU - Turgay ŞİŞMAN , Hakan AŞKIN , Hasan TÜRKEZ , Hakan ÖZKAN , Ümit İNCEKARA , Suat ÇOLAK Y1 - 2015 PY - 2015 N1 - doi: 10.17216/LimnoFish-5000115825 DO - 10.17216/LimnoFish-5000115825 T2 - Journal of Limnology and Freshwater Fisheries Research JF - Journal JO - JOR SP - 75 EP - 81 VL - 1 IS - 2 SN - -2149-4428 M3 - doi: 10.17216/LimnoFish-5000115825 UR - http://dx.doi.org/10.17216/LimnoFish-5000115825 Y2 - 2018 ER -
EndNote %0 Journal of Limnology and Freshwater Fisheries Research Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts %A Turgay ŞİŞMAN , Hakan AŞKIN , Hasan TÜRKEZ , Hakan ÖZKAN , Ümit İNCEKARA , Suat ÇOLAK %T Determination of Nuclear Abnormalities in Peripheral Erythrocytes of the Frog Pelophylax ridibundus (Anura: Ranidae) sampled from Karasu River Basin (Turkey) for Pollution Impacts %D 2015 %J Journal of Limnology and Freshwater Fisheries Research %P -2149-4428 %V 1 %N 2 %R doi: 10.17216/LimnoFish-5000115825 %U 10.17216/LimnoFish-5000115825
ISNAD ŞİŞMAN, Turgay , AŞKIN, Hakan , TÜRKEZ, Hasan , ÖZKAN, Hakan , İNCEKARA, Ümit , ÇOLAK, Suat . "Karasu Nehri (Erzurum) Havzasında Yaşayan Pelophylax ridibundus (Anura: Ranidae) Kurbağalarında Kirliliğin Neden Olduğu Eritrositik Çekirdek Anormalliklerinin Tespiti". Journal of Limnology and Freshwater Fisheries Research 1 / 2 (Ağustos 2015): 75-81. http://dx.doi.org/10.17216/LimnoFish-5000115825