Van bölgesindeki diabetli hastalarda tip II serum antioksidan vitamin konsantrasyonlarının saptanması
Abstract
Bu çalışmada, Van bölgesinde yaşayan diabetik hastalardaki serum antioksidan vitamin vitamin C, vitamin E, pkaroten, retinol konsantrasyonları araştırıldı. Materyal olarak otuz beş diabetik ve yirmi sağlıklı insanın kan örnekleri kullanıldı. Serum vitamin konsantrasyonları spektrofotometrik olarak ölçüldü. Diabetik ve kontrol gruplarında sırasıyla vitamin C 0,49 ± 0,18 - 0,94 ±0,16 mg/dl; vitamin E 0,94 ± 0,09 - 0,73 ± 0,07 mg/dl; P-karoten 62,69 ± 2,70 - 62,90 ± 4,07 pg/dl; retinol 153,77 ± 7,56 - 182,28 ± 15,08 pg/dl; ayrıca glukoz 258,09 ± 12,49 - 95,15 ±1,61 mg/dl, kolesterol 217,75 ± 10,67 - 204,50 ± 10,45 mg/dl olarak saptandı. Diabetli hastalarda kontrol grubuna göre vitamin C, retinol ve p-karoten konsantrasyonlarının düşük, vitamin E ve kolesterolün ise yüksek olduğu belirlendi. Diabet ve kontrol grupları arasındaki bu farklar istatistik olarak önemli değildi. Diabetli hastalardaki kan glukoz seviyeleri ise istatistiksel olarak önemli oranda yüksek bulundu p
Keywords
References
- Champe PC, Harvey RA: Biyokimya, Lippincott’s Illustrated reviews serisinden, çev. Editörleri: Tokullugil A, Dirican M, Ulukaya E. İkinci baskı, Nobel Tıp Kitabevleri, İstanbul, (1997).
- Granado F, Olmedilla B, Gil-Maıtinez E, Blanco I, Millan I, Rojas-Hidalgo E: Carotenoids, retinol and tocopherols in patients with insulin-dependent diabetes mellitus and their immediate relatives. Clin Sci 94(2): 189-195, (1998).
- Ramachandran K: Beta carotene levels in diabetes mellitus. J Pharmacol 31:1089-1100, (1981).
- Sjostrand SE: Pharmacological properties of dehydroascorbic acid and ascorbic acid; effects on the Central and peripheral nervous systems in experimental animals. Açta Physiol Scan 358(supp): 1- 79, (1970).
- Som S, Basa S, Muhkeıjee D, Deb S, Choudhury PR, Mukherjee S, Chatterje SN, Chatterje IB: Ascorbic acid metabolism in diabetes mellitus. Metabolism 30:572-577, (1981).
- Chen MS, Hutchinson ML, Pecoıara RE, Lee WYL, Labbe RF: Hyperglicemia induced intracellular depletion of ascorbic acid in human mononuclear leucocytes. Diabetes 32:1078-1081, (1983).
- Mooradian AD: The effect of ascorbate and dahydroascorbate on tissue uptake of glucose. Diabetes 36:1001-1004, (1987).
- Jennings PE, Chirico S, Jones AF, Lunec J, Bamett AH: Vitamin C metabolites and micıoangiopathy in diabetes mellitus. Diabetes Res 6:151-154, (1987).
- Vataserry GT, Morley HE, Kuskowski MA. Vitamin E in plasma and platelets of human diabetic patients and control subjects. Am J Clin Nutr 1983; 37:641-644.
- Özden I, Deniz G, Tasalı E, Ulusaraç A, Altuğ T, Büyükdevrim S: The effect of Tvitamin E on glycosylated hemoglobin levels in diabetic rats, a preliminary report. Diabetes Res 12:123-124, (1989).
- Asayama K, Nakane T, Uchida N, Hayashibe H, Dobashi K, Nakazavva S: Serum antioxidant status in streptozotocin- induced diabetic rat. Horm Metab Res 26(7): 313-315, (1994).
- Giugliano D, Ceriello A, Paolisso G: Diabetes mellitus, hypertension, and cardiovascular disease: which role for oxidative stress? Metabolism 44(3): 363-368, (1995).
- Martinek R: Method for determination of vitamin E (total tocopherol) in serum. Clin Chem 10(12): 1078-1086,(1964).
- Mert N: Veteriner Klinik Biyokimya. U.Ü. Güçlendirme Vakfı Yayını. No: 12, Bursa , (1996).
- Omaye ST, Tumbul JD, Savbeılich HE: Ascorbic acid analysis. II.determination dinitrophenylhidrazine.Selected methods for determination of ascorbic acid in animal cells, tissues and fluids. Meth Enzymol (62):7-8, (1979).
- Suzuki I, Katoh NA: simple and cheap methods for measuring serum vitamin A in cattle using spectrophototmeter. Jpn J Vet Sci 52(6); 1281-1283, (1990)/
- Düzgüneş O, Kesici T, Kavuncu O, Gürbüz F: Araştırma ve Deneme Metotları (İstatistik Metotları-1) AÜ Zir Fak Yay No: 1021, Ankara, (1987).
- Seifter E, Readawer J, Stratford F, Kambosos D, Leverson SM: İmpaired wound healing in stertocin diabetes; prevention by supplementation vitamin A. Ann Suıg 194:42-50, (1981).
- Olmedilla B, Granado F, Gil-Maıtinez E, Blanco I, Rojas- Hidalgo E: Reference values for retinol, tocopherol, and main carotenoids in serum of control and insulin-dependent diabetic Spanish subjects. Clin Chem 43(6 Pt 1): 1066-1071, (1997).
- Reunanen A, Knekt P, Aaran RK. Aıomaa A: Serum antioxidants and risk of non-insulin dependent diabetes mellitus. Eur J Clin Nutr 52(2):89-93, (1998).
- Martinoli L, Di Felice M, Seghieri G, Ciuti M. De Giorgio LA, Fazzini A, Gori R,Anichini R, Fıanconi FC: Plasma rctinol and alpha-tocopherol concentrations in insulin-dependent diabetes mellitus: thcir relationship to microvascular complications.Int J Vitam Nutr Res 63(2):87-92, (1993).
- Tuitoek PJ. Ziari S, Tsin AT, Rajotte RV, Suh M, Basu TK: Streptozotocin-induced diabetes in rats is associated with impaired metabolic availability of vitamin A (retinol). Br J Nutr 75(4): 615-22, (1996).
- Akkus I, Kalak S, Vural H, Çağlayan O, Menekşe E, Can G, Durmuş B: Leukocyte lipid pcroxidation, superoxide dismutase, glutathione peroxidase and serum and leukocyte vitamin C levels of patients with typc II diabetes mellitus. Clin Chim Açta 244(2): 221-227, (1996).
- Seghieri G, Martinoli L, di Felice M, Anichini R, Fazzini A, Ciuti M, Miceli M, Gaspa L, Franconi F: Plasma and platelet ascorbate pools and lipid peroxidation in insulin-dependent diabetes mellitus. Eur J Clin Invest 28(8):659-663, (1996).
- Will JC, Ford ES, Bowman BA: Serum vitamin C concentrations and diabetes: findings from the Third National Health and Nutrition Examination Survey, 1988-1994. Am J Clin Nutr 70(1 ):49-52, (1999).
- Newill A, Habibzadeh N, Bishop N, Schorah CJ: Plasma levels of vitamin C component in diabetic subjects. Ann Clin Biochem 21:488-490,(1984).
- Cinaz P, Hasanoglu A, Bideci A, Biberoglu G: Plasma and erythrocyte vitamin E levels in children with insulin dependent diabetes mellitus. J Pediatr Endocrinol Metab 12(2): 193-196, (1999).
- Paolisso G, D’Amore A, Balbi V, Volpe C, Galzerano D, Giugliano D, Sgambato S, Varricchio M, D'Onofrio F: Plasma vitamin C affects glucose homeostasis in healthy subjects and in non-insulin-dependent diabetics. Am J Physiol 7(4):261-268, (1994).
- Karpen LW, Cataland S, O’Dorisio TM, Panganamala RV: Production of 12-hydroxy eicosatetracnoic acid and vitamin e status in platelets from type I human diabetic subjects. Diabetes 34:526-531, (1985).
- Paolissa G, D’Amore A, Giugliano D, Ceriello A, Varricchio M, D'Onofrio F: Pharmacological doses of vitamin E improve insulin action in healthy subjects and non insulin dpendent diabetic patients. Am J Clin Nutr 57:650-656, (1993).
- Ndahimana J, Dorchy H, Vertongen F: Erythrocyte and plasma antioxidant activity in diabetes mellitus type I. Presse-Med 25(5): 188-192, (1996).
- Kunisaki M, Bursell SE, Umeda F, Nawata H, King GL: Normalization of diacylglycerol-pıotein kinase C activation by vitamin E in aorta of diabetic rats and cultured rat smooth muscle cells exposed to elevated glucose levels. Diabetes 43(11): 1372- 1377,(1997).
- Van De Woude MG, Van Gaar IF, Van De Woude MF, De- Leeuw IA: Vitamin E status in normocholesterolemic and hypercholestrolemic diabetic patients. Açta Diabetol Let 24:133- 139,(1987).
- Ginter E, Zdichynecb, Holzerova O, Ticha E, Kobza R, Koziakova M, Cerra O, Ozdin L, Hıuba F, Novakova V, Sasko E, Gaber M: Hypercholestrolemic effect of ascorbic acid in maturity onset diabetes mellitus. Int J Vitam Nutr Res 48:368- 373, (1978).
Determination of serum antioxidant vitamin concentration vitamin E, C, retinol, P-carotene in the patients with diabetes mellitus type II in Van region
Abstract
Diabetes mellitus is a metabolic occurences of a disease during the insuffıciency of the hormone of insuline. In this serum vitamin concentartion in diabetic patients in Van area were studied. Blood samples of 35 diabetic patients and 20 in good health were used as research materials. The levels of vitamin were determined by spectrophotometrically. Obtained results in the diabetic and control groups were; vitamin C 0,49 ± 0,18-0,94 ±0,16 mg/dl ; vitamin 0,94 ± 0,09- 0,73 ± 0,07 mg/dl ; p-carotene 62,69 ± 2,70 - 62,90 ± 4,07 pıg/dl ; retinol 153,77 ± 7,56 - 182,28 ± 15,08 pg/dl ; glucose 258,09 ± 12,49 - 95,15 ± 1,61 mg/dl , cholesterol 217,75 ± 10,67 and 204,50 ± 10,45 mg/dl respectively. In the patients diabetics, levels of vitamin C, retinol and P-carotene were found lower and levels of vitamin E was higher than control. But these differences weı*e not appreciably signifıcant betvveen the groups. In the patients diabetics, cholesterol levels were not significantly increased, but the amount of glucose of blood was significantly high p
Keywords
References
- Champe PC, Harvey RA: Biyokimya, Lippincott’s Illustrated reviews serisinden, çev. Editörleri: Tokullugil A, Dirican M, Ulukaya E. İkinci baskı, Nobel Tıp Kitabevleri, İstanbul, (1997).
- Granado F, Olmedilla B, Gil-Maıtinez E, Blanco I, Millan I, Rojas-Hidalgo E: Carotenoids, retinol and tocopherols in patients with insulin-dependent diabetes mellitus and their immediate relatives. Clin Sci 94(2): 189-195, (1998).
- Ramachandran K: Beta carotene levels in diabetes mellitus. J Pharmacol 31:1089-1100, (1981).
- Sjostrand SE: Pharmacological properties of dehydroascorbic acid and ascorbic acid; effects on the Central and peripheral nervous systems in experimental animals. Açta Physiol Scan 358(supp): 1- 79, (1970).
- Som S, Basa S, Muhkeıjee D, Deb S, Choudhury PR, Mukherjee S, Chatterje SN, Chatterje IB: Ascorbic acid metabolism in diabetes mellitus. Metabolism 30:572-577, (1981).
- Chen MS, Hutchinson ML, Pecoıara RE, Lee WYL, Labbe RF: Hyperglicemia induced intracellular depletion of ascorbic acid in human mononuclear leucocytes. Diabetes 32:1078-1081, (1983).
- Mooradian AD: The effect of ascorbate and dahydroascorbate on tissue uptake of glucose. Diabetes 36:1001-1004, (1987).
- Jennings PE, Chirico S, Jones AF, Lunec J, Bamett AH: Vitamin C metabolites and micıoangiopathy in diabetes mellitus. Diabetes Res 6:151-154, (1987).
- Vataserry GT, Morley HE, Kuskowski MA. Vitamin E in plasma and platelets of human diabetic patients and control subjects. Am J Clin Nutr 1983; 37:641-644.
- Özden I, Deniz G, Tasalı E, Ulusaraç A, Altuğ T, Büyükdevrim S: The effect of Tvitamin E on glycosylated hemoglobin levels in diabetic rats, a preliminary report. Diabetes Res 12:123-124, (1989).
- Asayama K, Nakane T, Uchida N, Hayashibe H, Dobashi K, Nakazavva S: Serum antioxidant status in streptozotocin- induced diabetic rat. Horm Metab Res 26(7): 313-315, (1994).
- Giugliano D, Ceriello A, Paolisso G: Diabetes mellitus, hypertension, and cardiovascular disease: which role for oxidative stress? Metabolism 44(3): 363-368, (1995).
- Martinek R: Method for determination of vitamin E (total tocopherol) in serum. Clin Chem 10(12): 1078-1086,(1964).
- Mert N: Veteriner Klinik Biyokimya. U.Ü. Güçlendirme Vakfı Yayını. No: 12, Bursa , (1996).
- Omaye ST, Tumbul JD, Savbeılich HE: Ascorbic acid analysis. II.determination dinitrophenylhidrazine.Selected methods for determination of ascorbic acid in animal cells, tissues and fluids. Meth Enzymol (62):7-8, (1979).
- Suzuki I, Katoh NA: simple and cheap methods for measuring serum vitamin A in cattle using spectrophototmeter. Jpn J Vet Sci 52(6); 1281-1283, (1990)/
- Düzgüneş O, Kesici T, Kavuncu O, Gürbüz F: Araştırma ve Deneme Metotları (İstatistik Metotları-1) AÜ Zir Fak Yay No: 1021, Ankara, (1987).
- Seifter E, Readawer J, Stratford F, Kambosos D, Leverson SM: İmpaired wound healing in stertocin diabetes; prevention by supplementation vitamin A. Ann Suıg 194:42-50, (1981).
- Olmedilla B, Granado F, Gil-Maıtinez E, Blanco I, Rojas- Hidalgo E: Reference values for retinol, tocopherol, and main carotenoids in serum of control and insulin-dependent diabetic Spanish subjects. Clin Chem 43(6 Pt 1): 1066-1071, (1997).
- Reunanen A, Knekt P, Aaran RK. Aıomaa A: Serum antioxidants and risk of non-insulin dependent diabetes mellitus. Eur J Clin Nutr 52(2):89-93, (1998).
- Martinoli L, Di Felice M, Seghieri G, Ciuti M. De Giorgio LA, Fazzini A, Gori R,Anichini R, Fıanconi FC: Plasma rctinol and alpha-tocopherol concentrations in insulin-dependent diabetes mellitus: thcir relationship to microvascular complications.Int J Vitam Nutr Res 63(2):87-92, (1993).
- Tuitoek PJ. Ziari S, Tsin AT, Rajotte RV, Suh M, Basu TK: Streptozotocin-induced diabetes in rats is associated with impaired metabolic availability of vitamin A (retinol). Br J Nutr 75(4): 615-22, (1996).
- Akkus I, Kalak S, Vural H, Çağlayan O, Menekşe E, Can G, Durmuş B: Leukocyte lipid pcroxidation, superoxide dismutase, glutathione peroxidase and serum and leukocyte vitamin C levels of patients with typc II diabetes mellitus. Clin Chim Açta 244(2): 221-227, (1996).
- Seghieri G, Martinoli L, di Felice M, Anichini R, Fazzini A, Ciuti M, Miceli M, Gaspa L, Franconi F: Plasma and platelet ascorbate pools and lipid peroxidation in insulin-dependent diabetes mellitus. Eur J Clin Invest 28(8):659-663, (1996).
- Will JC, Ford ES, Bowman BA: Serum vitamin C concentrations and diabetes: findings from the Third National Health and Nutrition Examination Survey, 1988-1994. Am J Clin Nutr 70(1 ):49-52, (1999).
- Newill A, Habibzadeh N, Bishop N, Schorah CJ: Plasma levels of vitamin C component in diabetic subjects. Ann Clin Biochem 21:488-490,(1984).
- Cinaz P, Hasanoglu A, Bideci A, Biberoglu G: Plasma and erythrocyte vitamin E levels in children with insulin dependent diabetes mellitus. J Pediatr Endocrinol Metab 12(2): 193-196, (1999).
- Paolisso G, D’Amore A, Balbi V, Volpe C, Galzerano D, Giugliano D, Sgambato S, Varricchio M, D'Onofrio F: Plasma vitamin C affects glucose homeostasis in healthy subjects and in non-insulin-dependent diabetics. Am J Physiol 7(4):261-268, (1994).
- Karpen LW, Cataland S, O’Dorisio TM, Panganamala RV: Production of 12-hydroxy eicosatetracnoic acid and vitamin e status in platelets from type I human diabetic subjects. Diabetes 34:526-531, (1985).
- Paolissa G, D’Amore A, Giugliano D, Ceriello A, Varricchio M, D'Onofrio F: Pharmacological doses of vitamin E improve insulin action in healthy subjects and non insulin dpendent diabetic patients. Am J Clin Nutr 57:650-656, (1993).
- Ndahimana J, Dorchy H, Vertongen F: Erythrocyte and plasma antioxidant activity in diabetes mellitus type I. Presse-Med 25(5): 188-192, (1996).
- Kunisaki M, Bursell SE, Umeda F, Nawata H, King GL: Normalization of diacylglycerol-pıotein kinase C activation by vitamin E in aorta of diabetic rats and cultured rat smooth muscle cells exposed to elevated glucose levels. Diabetes 43(11): 1372- 1377,(1997).
- Van De Woude MG, Van Gaar IF, Van De Woude MF, De- Leeuw IA: Vitamin E status in normocholesterolemic and hypercholestrolemic diabetic patients. Açta Diabetol Let 24:133- 139,(1987).
- Ginter E, Zdichynecb, Holzerova O, Ticha E, Kobza R, Koziakova M, Cerra O, Ozdin L, Hıuba F, Novakova V, Sasko E, Gaber M: Hypercholestrolemic effect of ascorbic acid in maturity onset diabetes mellitus. Int J Vitam Nutr Res 48:368- 373, (1978).
There are 34 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 18, 2000 |
| Published in Issue | Year 2000 Volume: 6 Issue: 1-2 - 2000 |
