Abstract
The DNA barcode of Bruchidius varius collected from Dilek Peninsula and Büyük Menderes Delta National Park (DPNP) in Aydın province of Turkey is given for the first time. As DNA barcode, mitochondrial cytochrome c oxidase subunit I (COI) with a length of approximately 628 bp is sequenced and an illustrative barcode is presented along with nucleotide composition of the target gene location. Nucleotide composition of the given sequence includes 38.63% T, 27.98% A, 17.01% C and 16.38% G and showing a strong AT bias (66.61%). GC percentage is 33.39%. BLAST tool in NCBI is used to check the taxonomic position of the sequenced sample. DNA barcoding result fully corresponds with the morphological identification of the samples.
Supporting Institution
TUBİTAK
Project Number
119Z544
Thanks
We sincerely thank to TUBİTAK for supporting this study (Project number: 119Z544).
References
- Southgate B.J., Biology of the Bruchidae. Annual Review of Entomology, 1979;24: 449-473.
- Anton K.W., Bruchinae. (Catalogue of Palaearctic Coleoptera, Vol. 6., Chrysomeloidea, Apollo Books, Stenstrup, Denmark: Ed. Löbl I, Smetana A), 2010; 347-353.
- Decelle J., Lodos N., Contribution to the knowledge of the legume weevils of Turkey (Coleoptera: Bruchidae). Bulletin et Annales de la Societe Royale Belge d’Entomologie, 1989;125: 163-212.
- Hebert, P.D.N, Cywinska, A., Ball, S.L. and de Waard, J.R., Biological identifications through DNA barcodes. Proceedings of the Royal Society B, 2003;270: 313–321.
- Hebert P.D., Stoeckle M.Y., Zemlak T.S., Francis C.M., Identification of birds through DNA barcodes. PLoS Biol., 2004;2: e312.
- Mohr K.H., Chrysomelidae. In: Die Käfer Mitteleuropas. 9. Cerambycidae, Chrysomelidae. Krefeld: Goecke and Evers, 1966;95–280.
- NCBI website [cited 2022 July 25]. Available from: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome.
- Borowiec L., The seed-beetles from Turkey (Coleoptera, Bruchidae). Polskie Pismo Entomologiczne, 1984;54: 295-301.
- Borowiec L, Anton K.W., Materials to the Knowledge of Seed Beetles of the Mediterranean Subregion (Coleoptera, Bruchidae). Annals of the Upper Silesian Museum in Bytom Entomology, 1993;4: 99-152.
Abstract
Dilek Yarımadası ve Büyük Menderes Deltası Milli Parkı’ndan (Aydın) toplanmış Bruchidius varius türüne ait DNA barkodu ülkemizden ilk kez verilmiştir. DNA barkodu olarak yaklaşık 628 bp uzunluğundaki mitokondriyal sitokrom c oksidaz altünite 1 (COI) geni kullanılmıştır. Ayrıca ilgili gen bölgesi sekansının nükleotid kompozisyonu verilmiştir. Nükleotid komposizyonunda kuvvetli bir AT yönelimi ((%66,61) görülmüş olup nükleotid oranları %38,63 T, %27,98 A, %17,01 C ve %16,38 G şeklindedir. GC oranı %33,39’dur. Dizilenen örneğin taksonomik durumu NCBI’da bulunan BLAST aracıyla kontrol edilmiştir. DNA barkodlama sonucu, örneklerin morfolojik teşhisi ile örtüşmektedir.
Project Number
119Z544
References
- Southgate B.J., Biology of the Bruchidae. Annual Review of Entomology, 1979;24: 449-473.
- Anton K.W., Bruchinae. (Catalogue of Palaearctic Coleoptera, Vol. 6., Chrysomeloidea, Apollo Books, Stenstrup, Denmark: Ed. Löbl I, Smetana A), 2010; 347-353.
- Decelle J., Lodos N., Contribution to the knowledge of the legume weevils of Turkey (Coleoptera: Bruchidae). Bulletin et Annales de la Societe Royale Belge d’Entomologie, 1989;125: 163-212.
- Hebert, P.D.N, Cywinska, A., Ball, S.L. and de Waard, J.R., Biological identifications through DNA barcodes. Proceedings of the Royal Society B, 2003;270: 313–321.
- Hebert P.D., Stoeckle M.Y., Zemlak T.S., Francis C.M., Identification of birds through DNA barcodes. PLoS Biol., 2004;2: e312.
- Mohr K.H., Chrysomelidae. In: Die Käfer Mitteleuropas. 9. Cerambycidae, Chrysomelidae. Krefeld: Goecke and Evers, 1966;95–280.
- NCBI website [cited 2022 July 25]. Available from: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome.
- Borowiec L., The seed-beetles from Turkey (Coleoptera, Bruchidae). Polskie Pismo Entomologiczne, 1984;54: 295-301.
- Borowiec L, Anton K.W., Materials to the Knowledge of Seed Beetles of the Mediterranean Subregion (Coleoptera, Bruchidae). Annals of the Upper Silesian Museum in Bytom Entomology, 1993;4: 99-152.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Research Article |
| Authors | |
| Project Number | 119Z544 |
| Publication Date | December 29, 2022 |
| Submission Date | July 25, 2022 |
| Acceptance Date | August 2, 2022 |
| Published in Issue | Year 2022 Volume: 6 Issue: 2 |