Research Article
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Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique

Year 2024, Volume: 50 Issue: 1, 20 - 27, 24.04.2024

Şeyma Korkmaz Hafizullah Sharifi Ayşen Demir Mülazımoğlu

https://doi.org/10.35238/sufefd.1456420

Abstract

In the present work, electrochemical and spectroelectrochemical behaviors of Serotonin (5-HT) were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Cyclic voltammetric experiments of 5-HT on graphene/coal tar pitch/pencil graphite electrode (GR/CTP/PGE) were carried out between 0.0 V and 1.8 V potential range at a scan rate of 100 mV s-1 with 20 cycles in non-aqueous media. Surface characterizations were performed using CV, EIS and scanning electron microscope (SEM). Effect of different pH values was investigated by square wave voltammetry (SWV) for determination of 5-HT. Optimization of accumulation time was determined using square wave adsorptive stripping voltammetry (SWAdSV) within potential range of -0.2 to +0.6 V. 5-HT standard solutions changing from 75 µM to 1.0 µM were prepared and the corresponding peak currents were measured. From the obtained data calibration equation was derived Ip = 0.0329C5-HT + 0.1511 with correlation coefficient (R2) 0.9958. LOD was 3.51x10-7 M and LOQ was 1.05x10-6 M.

Keywords

Serotonin Cyclic Voltammetry Sensor Electrode Square Wave Adsorptive Stripping Voltammetry

Supporting Institution

Research Foundation of Necmettin Erbakan University

Project Number

BAP-23DR10002

Thanks

We extend our gratitude to the Research Foundation of Necmettin Erbakan University, Konya-TURKEY (BAP-23DR10002) for their financial support of this project.

References

  • Albayrak, I., Erkal, A., Yavuz, S., Kariper, I. A., Mülazımoğlu, I. E., & Üstündağ, Z. (2017). Isophtalic acid terminated graphene oxide modified glassy carbon nanosensor electrode: Cd and Bi analysis in tap water and milk samples. International Journal of Food Properties, 20(7), 1558-1568. https://doi.org/10.1080/10942912.2016.1213743
  • Barnett, N. W., Hindson, B. J., & Lewis, S. W. (1998). Determination of 5-hydroxytryptamine (serotonin) and related indoles by flow injection analysis with acidic potassium permanganate chemiluminescence detection. Analytica Chimica Acta, 362(2-3), 131-139. https://doi.org/10.1016/S0003-2670(98)00058-0
  • Demir Mülazımoğlu, A., Yılmaz, E., & Mülazımoğlu, I. E. (2012). Dithiooxamide Modified Glassy Carbon Electrode for the Studies of Non-Aqueous Media: Electrochemical Behaviors of Quercetin on the Electrode Surface. Sensors, 12(4), 3916-3928. https://doi.org/10.3390/s120403916
  • Demir Mülazımoğlu, A. , & Mülazımoğlu, I. E. (2013). Electrochemical Behaviors of 2-Amino-3-Hydroxypyridine onto the Glassy Carbon Sensor Electrode: Simultaneous and Independent Determinations of Quercetin, Galangin, 3-Hydroxyflavone, and Chrysin. Food Analytical Methods, 6(3), 845-853. https://doi.org/10.1007/s12161-012-9492-5
  • Durmuş, N., & Mülazımoğlu, I. E. (2023). Quantitative Determination of Quercetin in Black Tea and Beet Juice using SWAdSV onto The Pencil Graphite Electrode Surface. Analytical & Bioanalytical Electrochemistry, 15(11), 924-937. https://doi.org/10.22034/abec.2023.709094
  • Islamoglu, N., Mülazımoglu, I. E., & Mülazımoğlu, A. D. (2023). Sensitive and selective determination of paracetamol in antipyretic children's syrup with a polyglycine modified glassy carbon electrode. Analytical Methods. https://doi.org/10.1039/d3ay00789h
  • Kema, I. P., de Vries, E. G. E., & Muskiet, F. A. J. (2000). Clinical chemistry of serotonin and metabolites. Journal of Chromatography B, 747(1-2), 33-48. https://doi.org/10.1016/S0378-4347(00)00341-8
  • Kim, S. K., Kim, D., & Jeon, S. (2012). Electrochemical determination of serotonin on glassy carbon electrode modified with various graphene nanomaterials. Sensors and Actuators B-Chemical, 174, 285-291. https://doi.org/10.1016/j.snb.2012.08.034
  • Koluaçık, E., Karabiberoglu, S. U., & Dursun, Z. (2018). Electrochemical Determination of Serotonin Using Pre-treated Multi-walled Carbon Nanotube-polyaniline Composite Electrode. Electroanalysis, 30(12), 2977-2987. https://doi.org/10.1002/elan.201800588
  • Korkmaz, Ş., Bosnali, W., Mülazımoğlu, I. E., & Mülazımoğlu, A. D. (2023). Voltammetric Determination of Acrylamide Using Coal Tar Pitch Modified Pencil Graphite Electrode by SWV. Food Analytical Methods, 16(11-12), 1738-1745. https://doi.org/10.1007/s12161-023-02540-2
  • Leau, S. A., Lete, C., Matei, C., & Lupu, S. (2023). Electrochemical Sensing Platform Based on Metal Nanoparticles for Epinephrine and Serotonin. Biosensors-Basel, 13(8). https://doi.org/10.3390/bios13080781
  • Li, J., & Lin, X. Q. (2007). Simultaneous determination of dopamine and serotonin on gold nanocluster/overoxidized-polypyrrole composite modified glassy carbon electrode. Sensors and Actuators B-Chemical, 124(2), 486-493. https://doi.org/10.1016/j.snb.2007.01.021
  • Motsaathebe, P. C., & Fayemi, O. E. (2021). Serotonin electrochemical detection in tomatoes at MWCNT-AONP nanocomposite modified electrode. Materials Research Express, 8(11). https://doi.org/10.1088/2053-1591/ac31fd Mülazımoğlu, I. E., & Yılmaz, E. (2010). Quantitative determination of phenol in natural decayed leaves using procaine modified carbon paste electrode surface by cyclic voltammetry. Desalination, 256(1-3), 64-69. https://doi.org/10.1016/j.desal.2010.02.014
  • Mumtaz, M., Narasimhachari, N., Friedel, R. O., Pandey, G. N., & Davis, J. M. (1982). Evaluation of Fluorometric Assay-Methods for Serotonin in Platelets, Plasma and Whole-Blood Samples by Comparison with Gc-Ms-Sim Technique. Research Communications in Chemical Pathology and Pharmacology, 36(1), 45-60. <Go to ISI>://WOS:A1982NL87400004
  • Özcan, A., & Ilkbas, S. (2015). Poly(pyrrole-3-carboxylic acid)-modified pencil graphite electrode for the determination of serotonin in biological samples by adsorptive stripping voltammetry. Sensors and Actuators B-Chemical, 215, 518-524. https://doi.org/10.1016/j.snb.2015.03.100
  • Szeitz, A., & Bandiera, S. M. (2018). Analysis and measurement of serotonin. Biomedical Chromatography, 32(1). https://doi.org/10.1002/bmc.4135
  • Üstündağ, I., & Erkal, A. (2017). Determination of Dopamine in the Presence of Ascorbic Acid on Digitonin-Doped Coal Tar Pitch Carbonaceous Electrode. Sensors and Materials, 29(1), 85-94. https://doi.org/10.18494/Sam.2017.1416
  • Yilmaz, C., Tas, N. G., Kocadagli, T., & Gökmen, V. (2019). Determination of serotonin in nuts and nut containing products by liquid chromatography tandem mass spectrometry. Food Chemistry, 272, 347-353. https://doi.org/10.1016/j.foodchem.2018.08.064

Kare Dalga Adsorptif Sıyırma Voltametrisi Tekniği Kullanılarak Grafen/Katran/Kalem Grafit Sensör Elektrot ile Serotonin’in Tayini ve Elektrokimyasal Özellikleri

Year 2024, Volume: 50 Issue: 1, 20 - 27, 24.04.2024

Şeyma Korkmaz Hafizullah Sharifi Ayşen Demir Mülazımoğlu

https://doi.org/10.35238/sufefd.1456420

Abstract

Bu çalışmada Serotonin’in (5-HT) elektrokimyasal ve spektroelektrokimyasal davranışları dönüşümlü voltametri (CV) ve elektrokimyasal impedans spektroskopisi (EIS) teknikleriyle incelenmiştir. Grafen/katran/kalem grafit elektrot (GR/CTP/PGE) üzerinde 5-HT’nin CV deneyleri, 0,0 V ile 1,8 V potansiyel aralığında sulu olmayan ortamda Ag/Ag+/(10 mM AgNO3) referans elektrotu kullanılarak 20 döngü ile 100 mV s-1 tarama hızında gerçekleştirildi. Yüzey karakterizasyonları CV, EIS ve taramalı elektron mikroskobu (SEM) kullanılarak yapıldı. 5-HT’nin tayininde farklı pH değerlerinin etkisi kare dalga voltametri (SWV) tekniği ile araştırıldı. Biriktirme süresinin optimizasyonu, -0,2 ile +0,6 V potansiyel aralığında kare dalga adsorptif sıyırma voltametrisi (SWAdSV) kullanılarak belirlendi. 75 µM’den 1,0 µM’ye kadar değişen 5-HT standart çözeltileri hazırlandı ve karşılık gelen pik akımları ölçüldü. Elde edilen verilerden kalibrasyon denklemi Ip = 0,0329C5-HT + 0,1511 ve korelasyon katsayısı (R2) 0,9958 olarak hesaplandı. LOD 3,51x10-7 M ve LOQ 1,05x10-6 M olarak belirlendi.

Keywords

Serotonin Dönüşümlü Voltametri Sensör Elektrot Kare Dalga Adsorptif Sıyırma Voltametrisi

Project Number

BAP-23DR10002

References

  • Albayrak, I., Erkal, A., Yavuz, S., Kariper, I. A., Mülazımoğlu, I. E., & Üstündağ, Z. (2017). Isophtalic acid terminated graphene oxide modified glassy carbon nanosensor electrode: Cd and Bi analysis in tap water and milk samples. International Journal of Food Properties, 20(7), 1558-1568. https://doi.org/10.1080/10942912.2016.1213743
  • Barnett, N. W., Hindson, B. J., & Lewis, S. W. (1998). Determination of 5-hydroxytryptamine (serotonin) and related indoles by flow injection analysis with acidic potassium permanganate chemiluminescence detection. Analytica Chimica Acta, 362(2-3), 131-139. https://doi.org/10.1016/S0003-2670(98)00058-0
  • Demir Mülazımoğlu, A., Yılmaz, E., & Mülazımoğlu, I. E. (2012). Dithiooxamide Modified Glassy Carbon Electrode for the Studies of Non-Aqueous Media: Electrochemical Behaviors of Quercetin on the Electrode Surface. Sensors, 12(4), 3916-3928. https://doi.org/10.3390/s120403916
  • Demir Mülazımoğlu, A. , & Mülazımoğlu, I. E. (2013). Electrochemical Behaviors of 2-Amino-3-Hydroxypyridine onto the Glassy Carbon Sensor Electrode: Simultaneous and Independent Determinations of Quercetin, Galangin, 3-Hydroxyflavone, and Chrysin. Food Analytical Methods, 6(3), 845-853. https://doi.org/10.1007/s12161-012-9492-5
  • Durmuş, N., & Mülazımoğlu, I. E. (2023). Quantitative Determination of Quercetin in Black Tea and Beet Juice using SWAdSV onto The Pencil Graphite Electrode Surface. Analytical & Bioanalytical Electrochemistry, 15(11), 924-937. https://doi.org/10.22034/abec.2023.709094
  • Islamoglu, N., Mülazımoglu, I. E., & Mülazımoğlu, A. D. (2023). Sensitive and selective determination of paracetamol in antipyretic children's syrup with a polyglycine modified glassy carbon electrode. Analytical Methods. https://doi.org/10.1039/d3ay00789h
  • Kema, I. P., de Vries, E. G. E., & Muskiet, F. A. J. (2000). Clinical chemistry of serotonin and metabolites. Journal of Chromatography B, 747(1-2), 33-48. https://doi.org/10.1016/S0378-4347(00)00341-8
  • Kim, S. K., Kim, D., & Jeon, S. (2012). Electrochemical determination of serotonin on glassy carbon electrode modified with various graphene nanomaterials. Sensors and Actuators B-Chemical, 174, 285-291. https://doi.org/10.1016/j.snb.2012.08.034
  • Koluaçık, E., Karabiberoglu, S. U., & Dursun, Z. (2018). Electrochemical Determination of Serotonin Using Pre-treated Multi-walled Carbon Nanotube-polyaniline Composite Electrode. Electroanalysis, 30(12), 2977-2987. https://doi.org/10.1002/elan.201800588
  • Korkmaz, Ş., Bosnali, W., Mülazımoğlu, I. E., & Mülazımoğlu, A. D. (2023). Voltammetric Determination of Acrylamide Using Coal Tar Pitch Modified Pencil Graphite Electrode by SWV. Food Analytical Methods, 16(11-12), 1738-1745. https://doi.org/10.1007/s12161-023-02540-2
  • Leau, S. A., Lete, C., Matei, C., & Lupu, S. (2023). Electrochemical Sensing Platform Based on Metal Nanoparticles for Epinephrine and Serotonin. Biosensors-Basel, 13(8). https://doi.org/10.3390/bios13080781
  • Li, J., & Lin, X. Q. (2007). Simultaneous determination of dopamine and serotonin on gold nanocluster/overoxidized-polypyrrole composite modified glassy carbon electrode. Sensors and Actuators B-Chemical, 124(2), 486-493. https://doi.org/10.1016/j.snb.2007.01.021
  • Motsaathebe, P. C., & Fayemi, O. E. (2021). Serotonin electrochemical detection in tomatoes at MWCNT-AONP nanocomposite modified electrode. Materials Research Express, 8(11). https://doi.org/10.1088/2053-1591/ac31fd Mülazımoğlu, I. E., & Yılmaz, E. (2010). Quantitative determination of phenol in natural decayed leaves using procaine modified carbon paste electrode surface by cyclic voltammetry. Desalination, 256(1-3), 64-69. https://doi.org/10.1016/j.desal.2010.02.014
  • Mumtaz, M., Narasimhachari, N., Friedel, R. O., Pandey, G. N., & Davis, J. M. (1982). Evaluation of Fluorometric Assay-Methods for Serotonin in Platelets, Plasma and Whole-Blood Samples by Comparison with Gc-Ms-Sim Technique. Research Communications in Chemical Pathology and Pharmacology, 36(1), 45-60. <Go to ISI>://WOS:A1982NL87400004
  • Özcan, A., & Ilkbas, S. (2015). Poly(pyrrole-3-carboxylic acid)-modified pencil graphite electrode for the determination of serotonin in biological samples by adsorptive stripping voltammetry. Sensors and Actuators B-Chemical, 215, 518-524. https://doi.org/10.1016/j.snb.2015.03.100
  • Szeitz, A., & Bandiera, S. M. (2018). Analysis and measurement of serotonin. Biomedical Chromatography, 32(1). https://doi.org/10.1002/bmc.4135
  • Üstündağ, I., & Erkal, A. (2017). Determination of Dopamine in the Presence of Ascorbic Acid on Digitonin-Doped Coal Tar Pitch Carbonaceous Electrode. Sensors and Materials, 29(1), 85-94. https://doi.org/10.18494/Sam.2017.1416
  • Yilmaz, C., Tas, N. G., Kocadagli, T., & Gökmen, V. (2019). Determination of serotonin in nuts and nut containing products by liquid chromatography tandem mass spectrometry. Food Chemistry, 272, 347-353. https://doi.org/10.1016/j.foodchem.2018.08.064
There are 18 citations in total.

Details

Primary Language English
Subjects Electroanalytical Chemistry, Sensor Technology
Journal Section Research Articles
Authors

Şeyma Korkmaz 0000-0002-5445-7239

Hafizullah Sharifi 0000-0002-9317-5461

Ayşen Demir Mülazımoğlu 0000-0001-5780-3056

Project Number BAP-23DR10002
Publication Date April 24, 2024
Submission Date March 21, 2024
Acceptance Date March 30, 2024
Published in Issue Year 2024 Volume: 50 Issue: 1

Cite

APA Korkmaz, Ş., Sharifi, H., & Demir Mülazımoğlu, A. (2024). Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 50(1), 20-27. https://doi.org/10.35238/sufefd.1456420
AMA Korkmaz Ş, Sharifi H, Demir Mülazımoğlu A. Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique. sufefd. April 2024;50(1):20-27. doi:10.35238/sufefd.1456420
Chicago Korkmaz, Şeyma, Hafizullah Sharifi, and Ayşen Demir Mülazımoğlu. “Electrochemical Properties and Determination of Serotonin With Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode Using Square Wave Adsorptive Stripping Voltammetry Technique”. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 50, no. 1 (April 2024): 20-27. https://doi.org/10.35238/sufefd.1456420.
EndNote Korkmaz Ş, Sharifi H, Demir Mülazımoğlu A (April 1, 2024) Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 50 1 20–27.
IEEE Ş. Korkmaz, H. Sharifi, and A. Demir Mülazımoğlu, “Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique”, sufefd, vol. 50, no. 1, pp. 20–27, 2024, doi: 10.35238/sufefd.1456420.
ISNAD Korkmaz, Şeyma et al. “Electrochemical Properties and Determination of Serotonin With Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode Using Square Wave Adsorptive Stripping Voltammetry Technique”. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 50/1 (April 2024), 20-27. https://doi.org/10.35238/sufefd.1456420.
JAMA Korkmaz Ş, Sharifi H, Demir Mülazımoğlu A. Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique. sufefd. 2024;50:20–27.
MLA Korkmaz, Şeyma et al. “Electrochemical Properties and Determination of Serotonin With Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode Using Square Wave Adsorptive Stripping Voltammetry Technique”. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, vol. 50, no. 1, 2024, pp. 20-27, doi:10.35238/sufefd.1456420.
Vancouver Korkmaz Ş, Sharifi H, Demir Mülazımoğlu A. Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique. sufefd. 2024;50(1):20-7.
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Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.

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