Research Article
Year 2017,
Volume: 3 Issue: 3, 175 - 182, 29.12.2017
Abstract
Streptococcus parauberis alfa hemolitik, gram pozitif
kokoid bir bakteri olup Streptococcaceae familyasına
aittir. Bu bakteri kültür
balıklarında yoğun üretimden kaynaklı çok önemli bir hastalık olan
streptokokozise neden olmaktadır ve balık çiftliği endüstrisinde önemli bir
ekonomik kayba neden olmaktadır.
Çalışmamızda Eğirdir gölü balıklarından (Sander lucioperca, Carassius
gibelio, Cyprinus carpio) ve
kültür balığı türü olan (Oncorhynchus
mykiss, Walbaum)’dan 37 tane laktik asit bakterisi izole edilmiştir. Laktik
asit bakterilerinin tanımlanması için MRS, TSA ve M17 agardan izole edilen 37
izolatın fenotipik ve morfolojik karakterleri koloni morfolojisi, hücre
morfolojisi, hareketlilik, gram boyama, katalaz ve oksidaz üretimiyle yapılmıştır.
Diğer biyokimyasal özellikler de Bergey’s Manual of Systematic Bacteriology’e
göre yapılmıştır. İzole edilen bakterilerinden 25 tanesi S. parauberis, 2 tanesi Vagococcus
sp., 2 tanesi Lactococcus garvieae ve
5 tanesi de Lactococcus lactis olarak
biyokimyasal yöntemler ve 16Sr RNA gen sekansıyla tanımlanmıştır fakat 3 tanesi
(F65, F49 ve F50) tanımlanamamıştır. Çalışmamızda S. parauberis’in izolasyon ve identifikasyonu ilk olarak
Türkiye’deki Eğirdir gölü balıklarından ve gökkuşağı alabalığından gerçekleştirildi.
References
- Baeck G.W, Kim J.H, Gomez D.K, Park S.C. 2006. Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju Island. J Vet Sci. 7(1):53–58. doi:10.4142/jvs.2006.7.1.53
- Bondad-Reantaso M.G, Subasinghe R.P, Arthur J.R, Ogawa K, Chinabut S, Adlard R, Tan Z, Shariff M. 2005. Disease and health management in Asian aquaculture. Vet Parasitol. 132(3-4) 249-272. doi:10.1016/j.vetpar.2005.07.005
- Didinen B.I, Kubilay A, Diler O, Ekici S, Onuk E.E, Findik A, 2011. First isolation of Vagococcus salmoninarum from cultured rainbow trout (Oncorhynchus mykiss, walbaum) broodstocks in Turkey. Bull Eur Ass Fish Pathol. 31(6): 235-243.
- Didinen B.I, Yardımcı B, Onuk E.E, Metin S, Yıldırım P. 2014. Naturally Lactococcus garvieae infection in rainbow trout (Oncorhyncus mykiss Walbaum, 1792): new histopathological observations, phenotypic and molecular identification, Revue Méd Vét. 165(1-2): 12-19.
- Diler O, Altun S, Adiloglu A, Kubik A, Isikli B.T. 2002. First occurrence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull Eur Ass Fish Pathol. 22(1): 21.
- Domenech A, Ferna´ndez Garayza´bal J.F, Pascual C, Garcia J.A. Cutuli M.T, Moreno M.A, Collins M.D, Dominguez L. 1996. Streptococcosis in cultured turbot, Scophthalmus maximus (L.), associated with Streptococcus parauberis. J Fish Dis. 19(1): 33-38. doi: 10.1111/j.1365-2761.1996.tb00117.x
- Eldar A, Y Bejerano, A Livoff, A Horovitcz, Bercovier H. 1995. Experimental streptococcal meningo-encephalitis in cultured fish. Vet Microbiol. 43(1): 33-40. doi:10.1016/0378-1135(94)00052-X
- Facklam RR, Elliott JA. 1995. Identification, classification and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev. 8(4): 479–495.
- FAO. 2016. Tha state of world fisheries and aquaculture, Rome, 200p.
- Haines A, T.Gauthier D, E.Nebergall E, D.Cole S, M.Nguyen K, W.Rhodes M, K.Vogelbein W. 2013. First report of Streptococcus parauberis in wild finfish from North America. Vet Microbiol. 166(1-2):270-275 doi:10.1016/j.vetmic.2013.05.002
- Hoshina T, Sano T, Morimot K, Jofre J. 1958. A streptococcus pathogenic to fish. J Tokyo Univ Fish. 44: 57-68.
- Kesarcodi-Watson A, Kaspar H, Lategan M.L. J, Gibson L. 2008. Probiotics in aquaculture: The need, principles and mechanism of action and screening processes, Aquaculture, 274(1): 1-14. doi:10.1016/j.aquaculture.2007.11.019
- Kia RE, Yadollah M. 2013. Detection and ıdentification of different Streptococcosis strains in farmed rainbow trout in Boyerahmad and Dena regions (North South of Iran), World Journal of Fish and Marine Sciences 5(3): 315-321. doi:10.5829/idosi.wjfms.2013.05.03.72123
- Kitao T, T Aoki, R Sakoh. 1981. Epizootic caused by betahaemolytic Streptococcus species in cultured freshwater fish. Fish Pathol. 15(3-4): 301-307. doi:10.3147/jsfp.15.301
- Kusuda K, Kawai K, Salati F, Banner CR, Fryer JL. 1991. Enterococcus seriolicida sp. nov., a fish pathogen Int. J Syst Bacteriol. 41(3-4): 406-409.
- Lämmler Ch, Abdulmawjood A, Danic G, Vaillant S, Weig R. 1998. Differentiation of Streptococcus uberis and Streptococcus parauberis by restriction fragment length polymorphism analysis of the 16S ribosomal RNA gene and further studies on serological properties. Med Sci Res. 26, 177– 179.
- Liu D. 2000. Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology. 38 (1): 471.
- Nho LW, Shin GW, Park SB, Jang HB, Cha IS, Ha MA, Kim YR, Park YK, S. Dalvi R, Kang BJ, Joh SJ. 2009. Phenotypic characteristics of Streptococcus iniae and Streptococcus parauberis isolated from olive founder (Paralichthys olivaceus) Fems Microbiol Lett. 293(1): 20–27. doi:10.1111/j.1574-6968.2009.01491.x
- Park-Ae M, Mun-Kwon G, Hwang-Jee Y, Jung-Sung H, Kim-Dong W, Jin-Young P, Kim J.S, Na Y.J, Kim M.Y, Kim D.S, Chae S.H, Seo J.S. 2013. Genome Sequence of Streptococcus parauberis Strain KCTC11980, Isolated from Diseased Paralichthys olivaceus, Genome Announc. 1(5):e00780-13. doi:10.1128/genomeA.00780-13
- Pitkälä A, Koort J, Björkroth J. 2008. Identification and Antimicrobial Resistance of Streptococcus uberisand Streptococcus parauberis Isolated from bovine milk samples. J. Dairy Sci. 91(10):4075–4081 doi:10.3168/jds.2008-1040
- Pourgholam R, Laluei F, Saeedi AA, Taghavi MJ, Safari R, Zahedi A. 2013. Identification of some streptococcus species isolated from rainbow trout (Oncorhynchus msykiss) in Iran by using molecular method. Journal of Novel Applied Sciences, 2 (S4): 1228-1233.
- Romalde JL, Ravelo C, Valdes I. Magarinos B, de la Fuente E, Martin CS, Avendano-Herrera R, Toranzo AE. 2008. Streptococcus phocae, an emerging pathogen for salmonid culture. Vet Microbiol. 130(1-2): 198–207. doi: 10.1016/j.vetmic.2007.12.021
- Salminen S. 1998. Demonstration of safety of probiotics. Int J Food Microbiol. 44: 93-106.
- Pérez-Sánchez T, Balcázar JL, García Y, Halaihel N, Vendrell D, de Blas I, Merrifield DL, Ruiz-Zarzuela I. 2011. Identification and characterization of lactic acid bacteria isolated from rainbow trout, Oncorhynchus mykiss (Walbaum), with inhibitory activity against Lactococcus garvieae. J Fish Dis. 34(7):499–507. doi: 10.1111/j.1365-2761.2011.01260.x
- Schleifer, KH. 1986. Gram-positive cocci. In Bergey’s Manual of Systematic Bacteriology. Sharpe, M.E. and Holt, J.G. (Eds), The Williams and Wilkins Co., Baltimore, Md. p. 999–1103.
- Soto LP. 2010. Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use. Vet Med Int. 2010: 1-7. doi:10.4061/2010/274987
- Subasinghe R, Soto D, Jia J. 2009. Global aquaculture and its role in sustainable development Rev Aquacult. 1(1): 2-9. doi:10.1111/j.1753-5131.2008.01002.x
- Toranzo A.E, Devesa S, Heinen P, Riaza A, Nu´n˜ ez S, Barja J.L. 1994. Streptococcosis in cultured turbot caused by an Enterococcus-like bacterium. Bull Eur Assoc Fish Pathol. 14(1): 19– 23.
- Toranzo T, Alicia Magarin˜os Beatriz, L Romalde Jesu´ s. 2005. A review of the main bacterial fish diseases in mariculture systems. Aquaculture 246 (1-4): 37 – 61. doi:10.1016/j.aquaculture.2005.01.002
- Williams A.M, Collins M.D. 1990. Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov. J Appl Bacteriol. 68(5): 485–490. doi:10.1111/j.1365-2672.1990.tb02900.x
- Yang W and Li A. 2009. Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii, Aquaculture, 294(1-2): 14–17. doi:10.1016/j.aquaculture.2009.05.018
- Zlotkin A, Hershko H, Eldar A. 1998a. Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Appl. Environ. Microbiol. 64(10): 4065– 4067.
- Zlotkin A, Eldar A, Ghittino C, Bercovier H. 1998b. Identification of Lactococcus garvieae by PCR. J. Clin. Microbiol. 36(4): 983– 985.
Year 2017,
Volume: 3 Issue: 3, 175 - 182, 29.12.2017
Abstract
Streptococcus
parauberis
is an alpha-hemolytic gram positive coccoid bacterium belonging to the Streptococcaceae
family. This bacterium cause streptococcosis is a major disease in cultured
fish due to the intensification of aquaculture and causes significant economic
losses in fish farm industry. In our study, we isolated a total of 37 lactic
acid bacteria from wild fish (Sander lucioperca, Carassius gibelio,
Cyprinus carpio) of Lake Eğirdir and cultured fish (Oncorhynchus
mykiss, Walbaum) of farm in Turkey. For the isolation of bacteria
phenotypic and biochemical characteristics of the 37 isolates obtained from the
colonies grown on MRS, TSA and M17 agar characterized by determining colony
morphology, cell morphology, motility, gram staining and the production of
cytochrome oxidase and catalase. Further biochemical characteristics were
determined using conventional tests according to Bergey’s Manual of Systematic
Bacteriology. From the isolated lactic acid bacteria 25 of were identified as S.
parauberis, 2 of as Vagococcus sp., 2 of as Lactococcus garvieae
and 5 of as Lactococcus lactis by culture-based, biochemical test and 16Sr
RNA gene sequencing techniques but 3 of them couldn’t (F65, F49 and F50)
identified. In this study, the main
objective of us is to identify S. parauberis with conventional and 16Sr
RNA gene sequencing techniques from wild fish in Lake Eğirdir and in cultured
fish in fish farm of Egirdir-Turkey.
References
- Baeck G.W, Kim J.H, Gomez D.K, Park S.C. 2006. Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju Island. J Vet Sci. 7(1):53–58. doi:10.4142/jvs.2006.7.1.53
- Bondad-Reantaso M.G, Subasinghe R.P, Arthur J.R, Ogawa K, Chinabut S, Adlard R, Tan Z, Shariff M. 2005. Disease and health management in Asian aquaculture. Vet Parasitol. 132(3-4) 249-272. doi:10.1016/j.vetpar.2005.07.005
- Didinen B.I, Kubilay A, Diler O, Ekici S, Onuk E.E, Findik A, 2011. First isolation of Vagococcus salmoninarum from cultured rainbow trout (Oncorhynchus mykiss, walbaum) broodstocks in Turkey. Bull Eur Ass Fish Pathol. 31(6): 235-243.
- Didinen B.I, Yardımcı B, Onuk E.E, Metin S, Yıldırım P. 2014. Naturally Lactococcus garvieae infection in rainbow trout (Oncorhyncus mykiss Walbaum, 1792): new histopathological observations, phenotypic and molecular identification, Revue Méd Vét. 165(1-2): 12-19.
- Diler O, Altun S, Adiloglu A, Kubik A, Isikli B.T. 2002. First occurrence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull Eur Ass Fish Pathol. 22(1): 21.
- Domenech A, Ferna´ndez Garayza´bal J.F, Pascual C, Garcia J.A. Cutuli M.T, Moreno M.A, Collins M.D, Dominguez L. 1996. Streptococcosis in cultured turbot, Scophthalmus maximus (L.), associated with Streptococcus parauberis. J Fish Dis. 19(1): 33-38. doi: 10.1111/j.1365-2761.1996.tb00117.x
- Eldar A, Y Bejerano, A Livoff, A Horovitcz, Bercovier H. 1995. Experimental streptococcal meningo-encephalitis in cultured fish. Vet Microbiol. 43(1): 33-40. doi:10.1016/0378-1135(94)00052-X
- Facklam RR, Elliott JA. 1995. Identification, classification and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev. 8(4): 479–495.
- FAO. 2016. Tha state of world fisheries and aquaculture, Rome, 200p.
- Haines A, T.Gauthier D, E.Nebergall E, D.Cole S, M.Nguyen K, W.Rhodes M, K.Vogelbein W. 2013. First report of Streptococcus parauberis in wild finfish from North America. Vet Microbiol. 166(1-2):270-275 doi:10.1016/j.vetmic.2013.05.002
- Hoshina T, Sano T, Morimot K, Jofre J. 1958. A streptococcus pathogenic to fish. J Tokyo Univ Fish. 44: 57-68.
- Kesarcodi-Watson A, Kaspar H, Lategan M.L. J, Gibson L. 2008. Probiotics in aquaculture: The need, principles and mechanism of action and screening processes, Aquaculture, 274(1): 1-14. doi:10.1016/j.aquaculture.2007.11.019
- Kia RE, Yadollah M. 2013. Detection and ıdentification of different Streptococcosis strains in farmed rainbow trout in Boyerahmad and Dena regions (North South of Iran), World Journal of Fish and Marine Sciences 5(3): 315-321. doi:10.5829/idosi.wjfms.2013.05.03.72123
- Kitao T, T Aoki, R Sakoh. 1981. Epizootic caused by betahaemolytic Streptococcus species in cultured freshwater fish. Fish Pathol. 15(3-4): 301-307. doi:10.3147/jsfp.15.301
- Kusuda K, Kawai K, Salati F, Banner CR, Fryer JL. 1991. Enterococcus seriolicida sp. nov., a fish pathogen Int. J Syst Bacteriol. 41(3-4): 406-409.
- Lämmler Ch, Abdulmawjood A, Danic G, Vaillant S, Weig R. 1998. Differentiation of Streptococcus uberis and Streptococcus parauberis by restriction fragment length polymorphism analysis of the 16S ribosomal RNA gene and further studies on serological properties. Med Sci Res. 26, 177– 179.
- Liu D. 2000. Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology. 38 (1): 471.
- Nho LW, Shin GW, Park SB, Jang HB, Cha IS, Ha MA, Kim YR, Park YK, S. Dalvi R, Kang BJ, Joh SJ. 2009. Phenotypic characteristics of Streptococcus iniae and Streptococcus parauberis isolated from olive founder (Paralichthys olivaceus) Fems Microbiol Lett. 293(1): 20–27. doi:10.1111/j.1574-6968.2009.01491.x
- Park-Ae M, Mun-Kwon G, Hwang-Jee Y, Jung-Sung H, Kim-Dong W, Jin-Young P, Kim J.S, Na Y.J, Kim M.Y, Kim D.S, Chae S.H, Seo J.S. 2013. Genome Sequence of Streptococcus parauberis Strain KCTC11980, Isolated from Diseased Paralichthys olivaceus, Genome Announc. 1(5):e00780-13. doi:10.1128/genomeA.00780-13
- Pitkälä A, Koort J, Björkroth J. 2008. Identification and Antimicrobial Resistance of Streptococcus uberisand Streptococcus parauberis Isolated from bovine milk samples. J. Dairy Sci. 91(10):4075–4081 doi:10.3168/jds.2008-1040
- Pourgholam R, Laluei F, Saeedi AA, Taghavi MJ, Safari R, Zahedi A. 2013. Identification of some streptococcus species isolated from rainbow trout (Oncorhynchus msykiss) in Iran by using molecular method. Journal of Novel Applied Sciences, 2 (S4): 1228-1233.
- Romalde JL, Ravelo C, Valdes I. Magarinos B, de la Fuente E, Martin CS, Avendano-Herrera R, Toranzo AE. 2008. Streptococcus phocae, an emerging pathogen for salmonid culture. Vet Microbiol. 130(1-2): 198–207. doi: 10.1016/j.vetmic.2007.12.021
- Salminen S. 1998. Demonstration of safety of probiotics. Int J Food Microbiol. 44: 93-106.
- Pérez-Sánchez T, Balcázar JL, García Y, Halaihel N, Vendrell D, de Blas I, Merrifield DL, Ruiz-Zarzuela I. 2011. Identification and characterization of lactic acid bacteria isolated from rainbow trout, Oncorhynchus mykiss (Walbaum), with inhibitory activity against Lactococcus garvieae. J Fish Dis. 34(7):499–507. doi: 10.1111/j.1365-2761.2011.01260.x
- Schleifer, KH. 1986. Gram-positive cocci. In Bergey’s Manual of Systematic Bacteriology. Sharpe, M.E. and Holt, J.G. (Eds), The Williams and Wilkins Co., Baltimore, Md. p. 999–1103.
- Soto LP. 2010. Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use. Vet Med Int. 2010: 1-7. doi:10.4061/2010/274987
- Subasinghe R, Soto D, Jia J. 2009. Global aquaculture and its role in sustainable development Rev Aquacult. 1(1): 2-9. doi:10.1111/j.1753-5131.2008.01002.x
- Toranzo A.E, Devesa S, Heinen P, Riaza A, Nu´n˜ ez S, Barja J.L. 1994. Streptococcosis in cultured turbot caused by an Enterococcus-like bacterium. Bull Eur Assoc Fish Pathol. 14(1): 19– 23.
- Toranzo T, Alicia Magarin˜os Beatriz, L Romalde Jesu´ s. 2005. A review of the main bacterial fish diseases in mariculture systems. Aquaculture 246 (1-4): 37 – 61. doi:10.1016/j.aquaculture.2005.01.002
- Williams A.M, Collins M.D. 1990. Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov. J Appl Bacteriol. 68(5): 485–490. doi:10.1111/j.1365-2672.1990.tb02900.x
- Yang W and Li A. 2009. Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii, Aquaculture, 294(1-2): 14–17. doi:10.1016/j.aquaculture.2009.05.018
- Zlotkin A, Hershko H, Eldar A. 1998a. Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Appl. Environ. Microbiol. 64(10): 4065– 4067.
- Zlotkin A, Eldar A, Ghittino C, Bercovier H. 1998b. Identification of Lactococcus garvieae by PCR. J. Clin. Microbiol. 36(4): 983– 985.
There are 33 citations in total.
Details
| Journal Section | Research Article |
|---|---|
| Authors | |
| Publication Date | December 29, 2017 |
| Published in Issue | Year 2017Volume: 3 Issue: 3 |