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Year 2022, Volume: 9 Issue: 4, 500 - 515, 31.12.2022

Cem Dirlik Hacer Kandemir Nurberat Çetin Senem Şen Begüm Güler Aynur Gürel

https://doi.org/10.54287/gujsa.1194822

Abstract

Keywords

Micropropagation Walnut Juglans regia L. Paradox In vitro

Supporting Institution

EBİLTEM-TTO

Thanks

ULUCEVİZ TARIM ÜRÜNLERİ A.Ş.

References

  • Açıksöz, U. (2020). Bandırma İlçesindeki Ceviz Fidanı Üretiminin Mevcut Durumunun İncelenmesi. MSc Thesis, Bursa Uludağ University.
  • Aslansoy, B. (2012). Sultandağı (Afyon) yöresi cevizlerinin (Juglans regia L.) seleksiyon yoluyla ıslahı üzerine araştırmalar. . MSc Thesis, Selçuk University.
  • Bosela, M. J., & Michler, C. H. (2008). Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: Evaluation of multiple nutrient formulations and cytokinin types. In Vitro Cellular and Developmental Biology - Plant, 44(4), 316-329. doi:10.1007/s11627-008-9114-5
  • Budak, Y. (2010). Ceviz Yetiştiriciliği. Samsun İl Tarım Müdürlüğü, Çiftçi Eğitimi ve Yayım Şubesi Yayınları, 20 p., Samsun. PDF
  • Caboni, E., & Damiano, C. (2006). In vitro propagation of walnut (Juglans regia L.): Critical factors for the induction of the rooting response. Acta Horticulturae, 705, 329-333. doi:10.17660/ActaHortic.2005.705.44
  • Caboni, E., & Lauri, P. (1995). Effetto di fattori chimici, fisici e trasformazione localizzata sulla radicazione in vitro di noce. Italus Hortus, 4(2), 49-53.
  • Dong, P., Lichai, Y., Qingming, W., & Ruisheng, G. (2007). Factors affecting rooting of in vitro shoots of walnut cultivars. The Journal of Horticultural Science and Biotechnology, 82(2), 223-226. doi:10.1080/14620316.2007.11512223
  • Driver, J. A., & Kuniyuki, A. H. (1984). In vitro propagation of Paradox Walnut Rootstock. Hortscience, 19(4). 507-509.
  • Er, E., Akyüz, B., Öztürk, A., & Serdar, Ü. (2017). Cevizde Çeşit Değiştirme: Kabukaltı Aşısı. Bahçe, 46(Special Issue-2), 307-312.
  • Fidancı, A. (2005). Şebin ve KR-2 ceviz çeşitlerinin in vitro’da hızlı çoğaltılma tekniklerinin belirlenmesi. Bahçe, 34(1), 239-246.
  • Fu, Y., Gu, F., & Wu, W. (2003). Sterilization of Carya illinoensis Explants in Tissue Culture. Journal of Anhui Agricultural University, 31(2), 169-172.
  • Gürel, A., Hayta, Ş., Nartop, P., Bayraktar, M., & Fedakar S. O. (2013). Bitki Hücre, Doku ve Organ Kültürü Uygulamaları. Ege Üniversitesi Yayınları Yayın No: 58.
  • Jay-Allemand, C., Capelli, P., & Cornu, D. (1992). Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing gelrite medium. Scientia Horticulturae, 51(3-4), 335-342. doi:10.1016/0304-4238(92)90132-V
  • Kamali, K., Majidi, E., & Zarghami, R. (2001). Micropropagation of GF-677 Rootstocks (Prunus amygdalus x P. persica). Seed and Plant, 17, 175-177.
  • Kepenek, K., & Kolağası, Z. (2016). Micropropagation of Walnut (Juglans regia L.). Acta Physica Polonica A, 130(1), 150-156. doi:10.12693/APhysPolA.130.150
  • Kumar, P. P., & Loh, C. S. (2012). Plant Tissue Culture for Biotechnology. In: A. Altman, & P. M. Hasegawa (Eds.), Plant Biotechnology and Agriculture-Prospect for the 21st Century (pp. 131-138) Academic Press. doi:10.1016/B978-0-12-381466-1.00009-2
  • Kyte, L. (1987). Plants from Test Tubes. An Introduction to Micropropagation. Timber.
  • Leal, D. R., Sánchez-Olate, M., Avilés, F., Materan, M. E., Uribe, M., Hasbún, R., & Rodríguez, R. (2007). Micropropagation of Juglans regia L. In: S. M. Jain, & H. Häggman (Eds.), Protocols for Micropropagation of Woody Trees and Fruits (pp. 381-390). Springer, Dordrecht. doi:10.1007/978-1-4020-6352-7_35
  • Leelavathy, S., & Sankar, P. D. (2016). Curbing the menace of contamination in plant tissue culture. Journal of Pure and Applied Microbiology, 10(3), 2145-2152.
  • Licea-Moreno, R., Fira, A., & Chocov, G. (2020). Micropropagation of valuable walnut genotypes for timber production: new advances and insights. Annals of Silvicultural Research, 44(1), 5-13. doi:10.12899/asr-1932
  • Lone, I. A. (2017). Effect of different growth regulator combinations on in vitro callusing of walnut (J. regia L.). An Asian Journal of Soil Science, 12(1), 203-209. doi:10.15740/HAS/AJSS/12.1/203-209
  • Meier-Dinkel, A., & Wenzlitschke, I. (2015). Micropropagation of mature Juglans hybrids. In: M. Beruto, & E. A. Ozudogru (Eds.), Proceedings of the VI International Symposium on Production and Establishment of Micropropagated Plants, ISHS Acta Horticulturae 1155, (pp. 85-92). doi:10.17660/ActaHortic.2017.1155.11
  • Murashige, G., & Skoog, F. (1962). A Revised Medium for Rapid Growthand Bioassays With Tobacco Tissue Culture. Physiologia Plantarum, 15(3), 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
  • Ray, S. S., & Ali, N. (2016). Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation. Brazilian Archives of Biology and Technology, 59(e16160485). doi:10.1590/1678-4324-2016160485
  • Ripetti, V., Kevers, C. & Gaspar, T. (1994). Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and in ethylene production. Advances in Horticultural Science, 8(1), 29-32.
  • Revilla, M. A., Majada, J., & Rodriguez, R. (1989). Walnut (Juglans regia L.) micropropagation. Annales des sciences forestières, INRA/EDP Sciences, 46(Suppl), 149s-151s. PDF
  • Rugini, E. (1984). In Vitro Propagation of Some Olive (Olea europaea sativa L.) Cultivars with Different Root-Ability and Medium Development Using Analytical Data from Developing Shoots and Embryos. Scientia Hotriculturae, 24(2), 123-134. doi:10.1016/0304-4238(84)90143-2
  • Saadat, Y. A., & Hennerty, M. J. (2002). Factors Affecting the Shoot Multiplication of Persian Walnut (Juglans regia L.). Scientia Horticulturae, 95(3), 251-260. doi:10.1016/S0304-4238(02)00003-1
  • Scaltsoyiannes, A., Tsoulpha, P., Panetsos, K. P., & Moulalis, D. (1997). Effect of Genotype on Micropropagation of Walnut Trees (Juglans regia). Silvae Genetica, 46(6), 326-332.
  • Schader, M. (2019). The Definition of Necrosis in Plants (Accessed: 25/11/2019) URL (https://sciencing.com/the-definition-of-necrosis-in-plants-12003546.html)
  • Stevens, M. E., & Pijut, P. M. (2018). Rapid in vitro shoot multiplication of the recalcitrant species Juglans nigra L. In Vitro Cellular & Developmental Biology-Plant, 54(3), 309-317. doi:10.1007/s11627-018-9892-3
  • Şen, S. M. (2011). Ceviz Yetiştiriciliği-Besin Değeri-Folklorü. ÜÇM yayıncılık, Samsun.
  • Şirin, E. (2014). Kaman 1 ve Kaman 5 ceviz çeşitlerinin (Juglans regia L.) mikroçoğaltımı. MSc Thesis, Gaziosmanpaşa University.
  • Tarinejad, A. (2013). Effects of disinfectants and antibiotics on contamination during propagation of walnut (Juglans regia L.). Research on Crops, 14(1), 219-225.
  • Tekielska, D., Peňázová, E., Kovács, T., Křižan, B., Čechová, J., & Eichmeier, A. (2019). Bacterial contamination of plant in vitro cultures in commercial production detected by high-throughput amplicon sequencing. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(4), 1005-1014. doi:10.11118/actaun201967041005
  • Tetsumura, T., Tsukuda, K., & Kawase, K. (2002). Micropropagation of Shinano Walnut (Juglans regia L.). Journal of the Japanese Society for Horticultural Science, 71(5), 661-663. doi:10.2503/jjshs.71.661
  • Tuan, P. N., Meier-Dinkel, A., Höltken, A. M., Wenzlitschke, I. & Winkelmann, T. (2017). Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro. In: M. Beruto, & E. A. Ozudogru (Eds.), Proceedings of the VI International Symposium on Production and Establishment of Micropropagated Plants, ISHS Acta Horticulturae 1155, (pp. 525-530). doi:10.17660/ActaHortic.2017.1155.77
  • Vahdati, K., Ashrafi, E. N., Ebrahimzadeh, H., & Mirmasoumi, M. (2009). Improved micropropagation of walnut (Juglans regia L.) on media optimized for growth based upon mineral content of walnut seed. In: M.-V. Hanke, F. Dunemann, & H. Flachowsky (Eds.), Proceedings of the I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008, ISHS Acta Horticulturae 839, (pp. 117-124). doi:10.17660/ActaHortic.2009.839.13
  • Varghese, N., & Joy, P. P. (2016). Plant tissue culture contaminants identification and its response to fumigation. Technical Report, 1-10.
  • Yiğit, D., Yiğit, N., Aktaş, E., & Özgen, U. (2009). Ceviz (Juglans regia L.)’in antimikrobiyal aktivitesi. Türk Mikrobiyoloji Cemiyeti Dergisi, 39(1-2), 7-11.

Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes

Year 2022, Volume: 9 Issue: 4, 500 - 515, 31.12.2022

Cem Dirlik Hacer Kandemir Nurberat Çetin Senem Şen Begüm Güler Aynur Gürel

https://doi.org/10.54287/gujsa.1194822

Abstract

The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the 'Paradox' (Juglans regia x J. hindsii) rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate (4 %). According to the results, SP4 medium provided the highest number of shoots per unit explant (1.05 shoots/explant) and 100 % shoot formation, SP2 medium provided the best mean shoot length (1.6 cm) and the mean number of nodes per unit explant (7.95 nodes/explant). Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod (3500 lux) for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite (1:1 v/v) and was rooting rate is 45.45 % in ¼DFe medium.

Keywords

Micropropagation Walnut Juglans regia L. Paradox In vitro

References

  • Açıksöz, U. (2020). Bandırma İlçesindeki Ceviz Fidanı Üretiminin Mevcut Durumunun İncelenmesi. MSc Thesis, Bursa Uludağ University.
  • Aslansoy, B. (2012). Sultandağı (Afyon) yöresi cevizlerinin (Juglans regia L.) seleksiyon yoluyla ıslahı üzerine araştırmalar. . MSc Thesis, Selçuk University.
  • Bosela, M. J., & Michler, C. H. (2008). Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: Evaluation of multiple nutrient formulations and cytokinin types. In Vitro Cellular and Developmental Biology - Plant, 44(4), 316-329. doi:10.1007/s11627-008-9114-5
  • Budak, Y. (2010). Ceviz Yetiştiriciliği. Samsun İl Tarım Müdürlüğü, Çiftçi Eğitimi ve Yayım Şubesi Yayınları, 20 p., Samsun. PDF
  • Caboni, E., & Damiano, C. (2006). In vitro propagation of walnut (Juglans regia L.): Critical factors for the induction of the rooting response. Acta Horticulturae, 705, 329-333. doi:10.17660/ActaHortic.2005.705.44
  • Caboni, E., & Lauri, P. (1995). Effetto di fattori chimici, fisici e trasformazione localizzata sulla radicazione in vitro di noce. Italus Hortus, 4(2), 49-53.
  • Dong, P., Lichai, Y., Qingming, W., & Ruisheng, G. (2007). Factors affecting rooting of in vitro shoots of walnut cultivars. The Journal of Horticultural Science and Biotechnology, 82(2), 223-226. doi:10.1080/14620316.2007.11512223
  • Driver, J. A., & Kuniyuki, A. H. (1984). In vitro propagation of Paradox Walnut Rootstock. Hortscience, 19(4). 507-509.
  • Er, E., Akyüz, B., Öztürk, A., & Serdar, Ü. (2017). Cevizde Çeşit Değiştirme: Kabukaltı Aşısı. Bahçe, 46(Special Issue-2), 307-312.
  • Fidancı, A. (2005). Şebin ve KR-2 ceviz çeşitlerinin in vitro’da hızlı çoğaltılma tekniklerinin belirlenmesi. Bahçe, 34(1), 239-246.
  • Fu, Y., Gu, F., & Wu, W. (2003). Sterilization of Carya illinoensis Explants in Tissue Culture. Journal of Anhui Agricultural University, 31(2), 169-172.
  • Gürel, A., Hayta, Ş., Nartop, P., Bayraktar, M., & Fedakar S. O. (2013). Bitki Hücre, Doku ve Organ Kültürü Uygulamaları. Ege Üniversitesi Yayınları Yayın No: 58.
  • Jay-Allemand, C., Capelli, P., & Cornu, D. (1992). Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing gelrite medium. Scientia Horticulturae, 51(3-4), 335-342. doi:10.1016/0304-4238(92)90132-V
  • Kamali, K., Majidi, E., & Zarghami, R. (2001). Micropropagation of GF-677 Rootstocks (Prunus amygdalus x P. persica). Seed and Plant, 17, 175-177.
  • Kepenek, K., & Kolağası, Z. (2016). Micropropagation of Walnut (Juglans regia L.). Acta Physica Polonica A, 130(1), 150-156. doi:10.12693/APhysPolA.130.150
  • Kumar, P. P., & Loh, C. S. (2012). Plant Tissue Culture for Biotechnology. In: A. Altman, & P. M. Hasegawa (Eds.), Plant Biotechnology and Agriculture-Prospect for the 21st Century (pp. 131-138) Academic Press. doi:10.1016/B978-0-12-381466-1.00009-2
  • Kyte, L. (1987). Plants from Test Tubes. An Introduction to Micropropagation. Timber.
  • Leal, D. R., Sánchez-Olate, M., Avilés, F., Materan, M. E., Uribe, M., Hasbún, R., & Rodríguez, R. (2007). Micropropagation of Juglans regia L. In: S. M. Jain, & H. Häggman (Eds.), Protocols for Micropropagation of Woody Trees and Fruits (pp. 381-390). Springer, Dordrecht. doi:10.1007/978-1-4020-6352-7_35
  • Leelavathy, S., & Sankar, P. D. (2016). Curbing the menace of contamination in plant tissue culture. Journal of Pure and Applied Microbiology, 10(3), 2145-2152.
  • Licea-Moreno, R., Fira, A., & Chocov, G. (2020). Micropropagation of valuable walnut genotypes for timber production: new advances and insights. Annals of Silvicultural Research, 44(1), 5-13. doi:10.12899/asr-1932
  • Lone, I. A. (2017). Effect of different growth regulator combinations on in vitro callusing of walnut (J. regia L.). An Asian Journal of Soil Science, 12(1), 203-209. doi:10.15740/HAS/AJSS/12.1/203-209
  • Meier-Dinkel, A., & Wenzlitschke, I. (2015). Micropropagation of mature Juglans hybrids. In: M. Beruto, & E. A. Ozudogru (Eds.), Proceedings of the VI International Symposium on Production and Establishment of Micropropagated Plants, ISHS Acta Horticulturae 1155, (pp. 85-92). doi:10.17660/ActaHortic.2017.1155.11
  • Murashige, G., & Skoog, F. (1962). A Revised Medium for Rapid Growthand Bioassays With Tobacco Tissue Culture. Physiologia Plantarum, 15(3), 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
  • Ray, S. S., & Ali, N. (2016). Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation. Brazilian Archives of Biology and Technology, 59(e16160485). doi:10.1590/1678-4324-2016160485
  • Ripetti, V., Kevers, C. & Gaspar, T. (1994). Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and in ethylene production. Advances in Horticultural Science, 8(1), 29-32.
  • Revilla, M. A., Majada, J., & Rodriguez, R. (1989). Walnut (Juglans regia L.) micropropagation. Annales des sciences forestières, INRA/EDP Sciences, 46(Suppl), 149s-151s. PDF
  • Rugini, E. (1984). In Vitro Propagation of Some Olive (Olea europaea sativa L.) Cultivars with Different Root-Ability and Medium Development Using Analytical Data from Developing Shoots and Embryos. Scientia Hotriculturae, 24(2), 123-134. doi:10.1016/0304-4238(84)90143-2
  • Saadat, Y. A., & Hennerty, M. J. (2002). Factors Affecting the Shoot Multiplication of Persian Walnut (Juglans regia L.). Scientia Horticulturae, 95(3), 251-260. doi:10.1016/S0304-4238(02)00003-1
  • Scaltsoyiannes, A., Tsoulpha, P., Panetsos, K. P., & Moulalis, D. (1997). Effect of Genotype on Micropropagation of Walnut Trees (Juglans regia). Silvae Genetica, 46(6), 326-332.
  • Schader, M. (2019). The Definition of Necrosis in Plants (Accessed: 25/11/2019) URL (https://sciencing.com/the-definition-of-necrosis-in-plants-12003546.html)
  • Stevens, M. E., & Pijut, P. M. (2018). Rapid in vitro shoot multiplication of the recalcitrant species Juglans nigra L. In Vitro Cellular & Developmental Biology-Plant, 54(3), 309-317. doi:10.1007/s11627-018-9892-3
  • Şen, S. M. (2011). Ceviz Yetiştiriciliği-Besin Değeri-Folklorü. ÜÇM yayıncılık, Samsun.
  • Şirin, E. (2014). Kaman 1 ve Kaman 5 ceviz çeşitlerinin (Juglans regia L.) mikroçoğaltımı. MSc Thesis, Gaziosmanpaşa University.
  • Tarinejad, A. (2013). Effects of disinfectants and antibiotics on contamination during propagation of walnut (Juglans regia L.). Research on Crops, 14(1), 219-225.
  • Tekielska, D., Peňázová, E., Kovács, T., Křižan, B., Čechová, J., & Eichmeier, A. (2019). Bacterial contamination of plant in vitro cultures in commercial production detected by high-throughput amplicon sequencing. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(4), 1005-1014. doi:10.11118/actaun201967041005
  • Tetsumura, T., Tsukuda, K., & Kawase, K. (2002). Micropropagation of Shinano Walnut (Juglans regia L.). Journal of the Japanese Society for Horticultural Science, 71(5), 661-663. doi:10.2503/jjshs.71.661
  • Tuan, P. N., Meier-Dinkel, A., Höltken, A. M., Wenzlitschke, I. & Winkelmann, T. (2017). Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro. In: M. Beruto, & E. A. Ozudogru (Eds.), Proceedings of the VI International Symposium on Production and Establishment of Micropropagated Plants, ISHS Acta Horticulturae 1155, (pp. 525-530). doi:10.17660/ActaHortic.2017.1155.77
  • Vahdati, K., Ashrafi, E. N., Ebrahimzadeh, H., & Mirmasoumi, M. (2009). Improved micropropagation of walnut (Juglans regia L.) on media optimized for growth based upon mineral content of walnut seed. In: M.-V. Hanke, F. Dunemann, & H. Flachowsky (Eds.), Proceedings of the I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008, ISHS Acta Horticulturae 839, (pp. 117-124). doi:10.17660/ActaHortic.2009.839.13
  • Varghese, N., & Joy, P. P. (2016). Plant tissue culture contaminants identification and its response to fumigation. Technical Report, 1-10.
  • Yiğit, D., Yiğit, N., Aktaş, E., & Özgen, U. (2009). Ceviz (Juglans regia L.)’in antimikrobiyal aktivitesi. Türk Mikrobiyoloji Cemiyeti Dergisi, 39(1-2), 7-11.
There are 40 citations in total.

Details

Primary Language English
Journal Section Agricultural Engineering
Authors

Cem Dirlik 0000-0002-5491-7119

Hacer Kandemir 0000-0001-9220-1317

Nurberat Çetin 0000-0003-0887-7758

Senem Şen 0000-0003-4678-6152

Begüm Güler 0000-0002-9970-2111

Aynur Gürel 0000-0002-7002-9752

Publication Date December 31, 2022
Submission Date October 26, 2022
Published in Issue Year 2022 Volume: 9 Issue: 4

Cite

APA Dirlik, C., Kandemir, H., Çetin, N., Şen, S., et al. (2022). Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes. Gazi University Journal of Science Part A: Engineering and Innovation, 9(4), 500-515. https://doi.org/10.54287/gujsa.1194822
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